Abstract：
:ERBB2/HER2/NEU, a member of the epidermal growth factor receptor family, is overexpressed in more than 25 % of non-small cell lung cancer and is considered to be a significant and independent prognostic factor in lung cancer. Here we generated a lung specific HER2 overexpressing transgenic mouse model. In this model, HER2 was driven by the human surfactant protein-C promoter to investigate the role of the HER2 oncogene in pulmonary carcinogenesis and progression. Notably, significant pathological changes, including lymphocyte infiltration and mesenchymal cells hyperplasia, were found in the lung tissue of transgenic mice aged from 4 to 12 months. The occurrence and severity of those lesions increased as the mice aged. Some inflammatory factors, such as tumor necrosis factor, interleukin 1 and interleukin 6, were upregulated in lung tissue of transgenic mice compared with that of wild-type mice, implying that long-term HER2 overexpression could induce serious lung inflammation and some precancerous lesions. This model would be useful for studying the mechanism of HER2 involvement in lung carcinogenesis and for understanding the relationship between carcinogenesis and inflammation.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Zeng S,Yang Y,Tan Y,Lu C,Pan Y,Chen L,Lu Gdoi
10.1007/s11033-012-1635-7subject
Has Abstractpub_date
2012-08-01 00:00:00pages
7911-7issue
8eissn
0301-4851issn
1573-4978journal_volume
39pub_type
杂志文章abstract：:Heparan sulfate proteoglycans (HSPGs) located at the cell surface and in the extracellular matrix of most animal tissues are proteoglycan coreceptors that carry heparan sulfate chains, and play a vital role in infections of many diseases. HSPGs are classified as glypican, syndecan, perlecan and agrin according to diff...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2340-2
更新日期：2013-03-01 00:00:00
abstract：:The influence of peptidyl-tRNA on the dissociation of yeast 80 S ribosomes into subunits was studied. For this purpose temperature-sensitive (ts) suppressor strain of yeast Saccharomyces cervisiae carrying a defect in peptide chain termination was used. It was found that peptidyl-tRNA did not influence the dissociatio...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00775964
更新日期：1978-06-16 00:00:00
abstract：:The transforming growth factor-β (TGF-β) signaling pathway is conserved across animals, and knowledge of its roles during the molt cycle in crustaceans is presently very limited. This study investigates the roles of the TGF-β receptor in molting-related muscle growth in Eriocheir sinensis. Using the RT-PCR and RACE te...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05108-8
更新日期：2020-01-01 00:00:00
abstract：:p53 helps in maintaining genomic stability by undergoing cellular arrest, DNA repair or cellular apoptosis during DNA damage. So, as to find the association of p53Arg 72 Pro towards lung carcinogenesis and overall survival of North Indian lung cancer patients, single nucleotide polymorphic variant (rs1042522) was anal...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-016-4072-1
更新日期：2016-12-01 00:00:00
abstract：:The aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hINS) expression in transgenic buffalo for therapeutic use. We have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitr...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3464-3
更新日期：2014-09-01 00:00:00
abstract：:Reactive oxygen species (ROS) play an important role in cell death induced by many different stimuli. Direct exposure of human hepatoma cell line SMMC-7221 to hydrogen peroxide (H2O2) can induce apoptosis characterized by morphological evidence and fragmentation of DNA assayed by terminal deoxynucleotidyl transferase ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1007003229171
更新日期：2000-03-01 00:00:00
abstract：:To investigate whether Annexin 1 can protect a retinal ganglion cells line (RGC-5) from apoptosis as induced by serum deprivation. Annexin 1 location in RGC-5 cells was determined using an indirect immunofluorescent assay. Expression of Annexin 1 in RGC-5 cultures deprived of serum for 0, 2 days was semi-quantified by...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1358-1
更新日期：2012-05-01 00:00:00
abstract：:Antimicrobial resistance is increasing around the world and the search for effective treatment options, such as new antibiotics and combination therapy is urgently needed. The present study evaluates oregano essential oil (OEO) antibacterial activities against reference and multidrug-resistant clinical isolates of Aci...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05989-0
更新日期：2020-12-01 00:00:00
abstract：:Combined treatment with niacin and chromium has been found to have a protective effect against oxidative damage to different tissues of hyperlipidemic rats. But its effects on vascular endothelial dysfunction are less clear. This study was performed to investigate the effect of combined treatment with niacin and chrom...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9309-1
更新日期：2009-07-01 00:00:00
abstract：:F-box proteins are key components of the ubiquitin (Ub)/26S proteasome pathway that mediates selective degradation of regulatory proteins involved in a wide variety of cellular processes affecting eukaryotic cells. In plants, F-box genes form one of the largest multigene superfamilies and control many important biolog...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0438-y
更新日期：2011-06-01 00:00:00
abstract：:Published data regarding the association between the apurinic/apyrimidinic endonuclease 1 (APE1) Asp148Glu polymorphism and bladder cancer risk showed inconclusive results. This meta-analysis of literatures was performed to draw a more precise estimation of the relationship. We systematically searched PubMed, Embase, ...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-012-2046-5
更新日期：2013-01-01 00:00:00
abstract：:Mollusks lack an adaptive immune system and rely solely on the innate immune response. The nuclear factor-kappa B (NF-κB) signaling pathway is one of the most important components of the innate immune system and its activity is regulated by physical interaction with the inhibitor of NF-κB (IκB) protein. The manila cla...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2196-5
更新日期：2013-02-01 00:00:00
abstract：:Development of a rapid and accurate quantification method for the detection of microRNAs (miRNAs) has been desired, in particular, when they are differently expressed in normal and pathological conditions. However, various methods for the quantification of small non-coding RNAs as well as miRNAs have been described. T...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2442-x
更新日期：2013-05-01 00:00:00
abstract：:MicroRNAs (miRNAs) are a newly identified class of non-coding small RNAs of about 21-24 nucleotides. They play important roles in multiple biological processes by degrading targeted mRNAs or repressing mRNA translation. To date, a total of 2,043 plant miRNAs are present in the miRNA Registry database (miRBase Release ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0260-6
更新日期：2011-03-01 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is a world-wide health problem and its incidence accounts for 1.9-3.5% of all malignant tumors. Transforming growth factor beta/Smads (TGF-beta/Smads) signaling pathway plays an important role in oncogenesis, but its function and molecular mechanisms in OSCC remain unclear. Expressi...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9256-x
更新日期：2009-05-01 00:00:00
abstract：:In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis-inducing protein, as a GST-fusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death. The HAP protein inhibited the bacterial growth within 30 min after inducting HAP expression. The transmission e...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/b:mole.0000043551.84883.c8
更新日期：2004-09-01 00:00:00
abstract：:The complete coding sequences of three porcine genes-SDHB, SNRPA and CRYBB1 were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the mouse or other mammals and highly homologous pig ESTs. These three genes were then deposited into GenBa...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9229-0
更新日期：2009-04-01 00:00:00
abstract：:BAP31 is a ubiquitously expressed integral membrane protein of the endoplasmic reticulum. BAP31 is involved in various biological and molecular processes, including protein transport, viral processing, apoptosis signaling, MHC 1 antigen processing and presentation, mitochondria and ER calcium regulation, and proteasom...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-020-06123-w
更新日期：2021-01-13 00:00:00
abstract：:Vitiligo is an acquired pigmentary disorder characterized by loss of epidermal melanocytes. A strong association at a single nucleotide polymorphism (SNP) rs11966200 within MHC region had been identified in a recent genome-wide association study of generalized vitiligo in Chinese Han population. This study aims to inv...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2491-9
更新日期：2013-06-01 00:00:00
abstract：:The novel dipstick DNA extraction method was tested for its reliability and usability for biological barcoding in comparison to a commercial kit and to a simplified isopropanol precipitation method using crayfish gill tissue. Following DNA extraction, the mitochondrial COI-gene was amplified in a PCR-reaction using a ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05083-0
更新日期：2019-12-01 00:00:00
abstract：:The multidrug and toxic compound extrusion (MATE) protein family is a newly discovered family of secondary transporters that extrude metabolic waste and a variety of antibiotics out of the cell using an electrochemical gradient of H+ or Na+ across the membrane. The main function of MATE gene family is to participate i...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4487-y
更新日期：2019-02-01 00:00:00
abstract：:The recombinant catalytic subunit of human protein kinase CK2 bas been mutagenised at the C-terminal region in an attempt to induce this tail to fold. We suppose in fact that this unstructured C-terminus just might be responsible for the high degradability of the human enzyme. On the basis of theoretical calculations ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1023934805326
更新日期：2003-06-01 00:00:00
abstract：:Cell free protein synthesis has become a powerful method for the high-throughput production of proteins that are difficult to express in living cells. The protein SAP2 of Fasciola hepatica (FhSAP2), which has demonstrated to be both, an excellent vaccine candidate against experimental fascioliasis and a good antigen f...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4251-3
更新日期：2018-10-01 00:00:00
abstract：:The interferon-inducible human MxA protein plays an important role in innate defense against an array of viruses. One might expect allelic diversity at the MxA locus to influence the timing and magnitude of its expression or even the range of viruses whose biological cycle is inhibited by the encoded product. Here we ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1708-7
更新日期：2012-08-01 00:00:00
abstract：:The 1,044 bp endo-1,4-β-xylanase gene of a hyperthermophilic Eubacterium, "Thermotoga petrophila RKU 1" (T. petrophila) was amplified, from the genomic DNA of donor bacterium, cloned and expressed in mesophilic host E. coli strain BL21 Codon plus. The extracellular target protein was purified by heat treatment followe...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1555-6
更新日期：2012-07-01 00:00:00
abstract：:Stable inheritance and expression of transgene are important parameters for successful use of a transgenic crop. We previously transformed a Bt cry1Ba3 gene into cabbage inbred line CA21-3. To evaluate the stability of our Bt cabbage lineages, transgene inheritance and expression were examined in four successive gener...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05344-3
更新日期：2020-04-01 00:00:00
abstract：:The lipoprotein lipase (LPL) gene can be considered a functional candidate gene that regulates fatty acid composition. In this study, genetic associations between fatty acid composition and exonic single nucleotide polymorphisms (SNPs) in the LPL gene were examined using 612 Korean cattle. We investigated the relation...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2389-y
更新日期：2013-04-01 00:00:00
abstract：:Current surgical reconstruction for soft tissue replacement involves lipotransfer to restore soft tissue replacements but is limited by survival and longevity of the fat tissue. Alternative approaches to overcome these limitations include using biodegradable scaffolds with stem cells with growth factors to generate so...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05297-7
更新日期：2020-03-01 00:00:00
abstract：:It has been demonstrated that ACSL3 and ACSL5 play important roles in fat metabolism. To investigate the primary functions of ACSL3 and ACSL5 and to evaluate their expression levels during goose fatty liver development, we cloned the ACSL3 and ACSL5 coding domain sequences (CDSs) of geese using RT-PCR and analyzed the...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3053-5
更新日期：2014-01-01 00:00:00
abstract：:Low levels of coenzyme Q10 (CoQ10) have been reported in the circulation of patients with breast cancer, particularly in metastatic features. Our objective was to study the correlation between plasma levels of CoQ10 and the tumoral expression levels of AMPK, PFKFB3, VEGF, and VEGFR2. This study was a part of consecuti...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05310-z
更新日期：2020-04-01 00:00:00
