Abstract:
:Reactive oxygen species (ROS) play an important role in cell death induced by many different stimuli. Direct exposure of human hepatoma cell line SMMC-7221 to hydrogen peroxide (H2O2) can induce apoptosis characterized by morphological evidence and fragmentation of DNA assayed by terminal deoxynucleotidyl transferase assay (TUNEL assay). Analysis of flow cytometry indicated that H2O2 can decrease the level of CD95(APO-1/Fas), and it is confirmed that H2O2 can also activate the differential expression of some specific gene such as p53 by means of RT-PCR technique. The results indicated that CD95 signal transduction system may be involved in the H2O2-induced apoptosis, and can regulate some specific genes associated with apoptosis in transcription and translation levels such as p53.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Huang C,Li J,Zheng R,Cui Kdoi
10.1023/a:1007003229171keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
1-11issue
1eissn
0301-4851issn
1573-4978journal_volume
27pub_type
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