Abstract：
:In plants, pathogenesis-related 1 protein (PR1) is considered as important defense protein. The production and accumulation of PR proteins in plants are one of the important responses to several biotic and abiotic stresses. In this regard, PR1 gene was isolated from Triticum turgidum ssp durum and was named as TdPR1.2. The amino acid sequence of TdPR1.2 protein showed 100%, 97.13%, and 44.41% with known PR1 proteins isolated from Triticum aestivum TdPR1-18, PRB1.2 of Aegilops tauschii subsp. tauschii and Arabidopsis thaliana respectively. qRT-PCR showed that TdPR1.2 was induced specially in leaves of durum wheat treated with Salicylic acid for 48 h. Besides, bioinformatic analysis showed that the durum wheat TdPR1.2 harbors a calmodulin binding domain located in it's C-terminal part and that this domain is conserved among different PR1 proteins isolated so far. However, no information is available about the regulation of PR genes by calmodulin and Ca2+ complex (CaM/Ca2+). Here, we showed that TdPR1.2 gene exhibits an antibacterial effect as revealed by the in vitro tests against 8 different bacteria and against the fungi Septoria tritici. In addition, we demonstrate for the first time that PR1 proteins are able to bind to CaM in a Ca2+-dependent manner via a GST-Pull down assay. Finally, in presence of Mn2+ cations, CaM/Ca2+ complex stimulated the antimicrobial effect of TdPR1.2. Such effects were not reported so far, and raise a possible role for CaM/Ca2+ complex in the regulation of plant PRs during cellular response to external signals.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Ghorbel M,Zribi I,Missaoui K,Drira-Fakhfekh M,Azzouzi B,Brini Fdoi
10.1007/s11033-020-06053-7subject
Has Abstractpub_date
2020-12-13 00:00:00eissn
0301-4851issn
1573-4978pii
10.1007/s11033-020-06053-7pub_type
杂志文章abstract：:Human type II inosine monophosphate dehydrogenase (IMPDH2) is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. Several Single Nucleotide Polymorphisms (SNP) affecting the IMPDH2 gene sequence have been reported with potent...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0668-z
更新日期：2011-11-01 00:00:00
abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) could infect porcine alveolar macrophages (PAM), and the CD169 and CD163 are identified as critical receptors on the surface of PAM, but whether the single nucleotide polymorphisms (SNPs) of these genes could influence the infection is remain unclear. In this...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1857-8
更新日期：2012-11-01 00:00:00
abstract：:To explore the relationship of Notch1 mutation in T-ALL with the survival rate of T-ALL patients. The PubMed database, the Cochrane Library, conference proceedings, EMBASE databases, and references of published trials and review articles were searched. Two reviewers independently assessed the quality of the trials and...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s11033-011-1424-8
更新日期：2012-05-01 00:00:00
abstract：:A chromosomal fragment containing purA, the nearest marker from the replication origin of the Bacillus subtilis chromosome, was highly purified as a complex containing at least proteins and being solubilized easily during cell lysis. The complex had a markedly higher sedimentation rate (70-120S) than the bulk of the s...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00356932
更新日期：1976-07-01 00:00:00
abstract：:MicroRNAs (miRNAs) may play important roles in SLE, but genetic polymorphisms of miRNAs and their relationships with various autoantibodies present in SLE patients remain unclear. Here, we report that 213 SLE patients and 209 healthy individuals of Chinese had been taken into this case-control studies, which had been ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0252-6
更新日期：2011-03-01 00:00:00
abstract：:Myocilin (MYOC) gene is expressed in many ocular tissues, including the trabecular meshwork, a specialized eye tissue essential in regulating intraocular pressure. Many mutations in MYOC have been detected in primary open-angle glaucoma (POAG). We investigated whether MYOC mutations contributed to the susceptibility t...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9667-3
更新日期：2010-01-01 00:00:00
abstract：:Circulating long noncoding RNAs as biomarkers of diseases have attracted increasing attention recently. However, circulating lncRNAs in hypertension is still unexplored niche. The levels of lncRNAs GAS5, NR024118, MRAK134679, AX765700 and MRNR026574 were measured in the serum and myocardium of hypertensive rats and no...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05086-x
更新日期：2019-12-01 00:00:00
abstract：:The aim of this study was to characterise the effects of ischemic preconditioning (IP) on heart function parameters (ΔST and ΔT), activities of serum creatine kinase (CK), lactate dehydrogenase (LDH), and levels of serum nitric oxide (NO), malondialdehyde (MDA), and myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor nec...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2677-1
更新日期：2013-10-01 00:00:00
abstract：:High Mobility Group Box 1 (HMGB1) is a nuclear protein participating in chromatin architecture and transcriptional regulation. Recently, there is increasing evidence that HMGB1 contributes to the pathogenesis of chronic inflammatory and autoimmune diseases due to its pro-inflammatory and immunostimulatory properties. ...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-009-9485-7
更新日期：2010-03-01 00:00:00
abstract：:It is necessary that genetic markers or biomarkers can be used to predict resistance towards a wide range of infectious diseases. In the present study, we estimated the potential markers and measured their relationship with heritabilities of a wide range of immune traits. Polymorphisms in exon 13 of Mx1, intron 25 of ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0992-y
更新日期：2012-03-01 00:00:00
abstract：:Start codon targeted (SCoT) polymorphic markers were used to assess genetic relationships among 64 grape varieties. Seventeen informative primers were selected from 36 SCoT primers based on their ability to produce clear and repeatable polymorphic and unambiguous bands among the varieties. A total of 131 bands were pr...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1329-6
更新日期：2012-05-01 00:00:00
abstract：:The CRISPR/Cas9 system now allows for unprecedented possibilities of genome editing. However, there are some limitations, including achieving efficient one-step multiple genome targeting to save costs, time, and ensure high quality. In the present study, we investigated the efficiency of one-step multiple gene modific...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05576-3
更新日期：2020-07-01 00:00:00
abstract：:Changes in host immunity and parasite resistance to drugs are among the factors that contribute to decreased efficacy of antiparasitic drugs such as the antimonial compounds pentamidine, amphotericin (AMP B) and miltefosine. Bioactive natural products could be alternatives for the development of new drugs to treat neg...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05887-5
更新日期：2020-11-01 00:00:00
abstract：:Recently genetics and epigenetics alterations have been found to be characteristic of malignancy and hence can be used as targets for detection of neoplasia. RAS association domain family protein 1A (RASSF1A) gene hypermethylation has been a subject of interest in recent researches on cancer breast patients. The aim o...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2837-3
更新日期：2014-01-01 00:00:00
abstract：:Sheep are valuable resources for the wool industry. Wool growth of Aohan fine wool sheep has cycled during different seasons in 1 year. Therefore, identifying genes that control wool growth cycling might lead to ways for improving the quality and yield of fine wool. In this study, we employed Agilent sheep gene expres...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3405-1
更新日期：2014-08-01 00:00:00
abstract：:Long-term ketamine abuse is known to affect the lower urinary tract and produce symptoms of cystitis. However, the pathophysiology and causative mechanism of the changes in bladder function remain unclear. The present study aimed to investigate the existence of ketamine-induced cystitis in a rat model and characterize...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3616-5
更新日期：2014-11-01 00:00:00
abstract：:Synthetic hexaploid (SH) wheat derived from crossing tetraploid durum wheat and diploid Aegilops tauschii provide germplasm for wheat improvement as the conventional wheat varieties possess very low genetic diversity. This study aims to identify diverse SH lines which can be used in breeding programs for transferring ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1943-y
更新日期：2013-01-01 00:00:00
abstract：:Studies of the refractive properties of Gramicidin A in absolute ethanol and in ethanol water mixtures showed that this peptide in solution undergoes a conformational transition resulting in species with different refractivity. Accordingly, the folded form of this peptide shows a lower specific refractive index increm...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00357540
更新日期：1975-07-01 00:00:00
abstract：:The binding of a cell nucleus stain, hematoxylin (HTL), to bovine serum albumin (BSA) was studied by spectroscopy including fluorescence spectra, UV-Visible absorption, circular dichroism (CD) spectra, synchronous and three-dimensional fluorescence spectra. The results indicated that the binding had led to static fluo...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9448-z
更新日期：2009-11-01 00:00:00
abstract：:JAK/STAT signaling pathway is involved in many diseases, including autoimmune diseases, which are characterized by a close interconnection between immune and bone system. JAK/STAT pathway is involved in bone homeostasis and plays an important role in proliferation and differentiation of some cell types, including oste...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-020-05910-9
更新日期：2020-11-01 00:00:00
abstract：:We utilized Percoll density gradient centrifugation to isolate and fractionate chloroplasts of Korean winter wheat cultivar cv. Kumgang (Triticum aestivum L.). The resulting protein fractions were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE) coupled with LTQ-FTICR mass spectrometry. This e...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1302-4
更新日期：2012-05-01 00:00:00
abstract：:LNX (Ligand of Numb-protein X) was originally isolated as a binding partner to the cell-fate Determinant Numb during development, and then identified to act as a RING finger-type E3 ubiquitin ligase for the ubiquitylation and degradation of Numb. LNX contains 4 PDZ domains which are proved to play a central role in or...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9754-5
更新日期：2010-06-01 00:00:00
abstract：:Breast cancer is one of the most frequently diagnosed cancers and the leading cause of cancer deaths among females across the world, accounting for 23 % (1.38 million) of total new cancer cases and 14 % (0.45 million) of the total cancer deaths in 2008. c-kit is expressed in mast cell growth factor, cellular migration...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1786-6
更新日期：2012-09-01 00:00:00
abstract：:To investigate mechanisms of rat glutathione S-transferase P1 gene (rGSTP1) expression regulation during chemical carcinogenesis. we studied enhancer elements located in the region between -2.5 kb to -2.2 kb. The region was upstream from the start site of transcription and was divided into two major fragments, GPEI an...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1011993604051
更新日期：2001-03-01 00:00:00
abstract：:Mucor circinelloides is a dimorphic fungal model for studying several biological processes including cell differentiation (yeast-mold transitions) as well as biodiesel and carotene production. The recent release of the first draft sequence of the M. circinelloides genome, combined with the availability of analytical m...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3818-x
更新日期：2015-03-01 00:00:00
abstract：:The aim of this study was to determine whether the Fcγ receptors (FCGRs) polymorphisms confer susceptibility to periodontitis in ethnically different populations. We did a literature search using PubMed and Embase, and conducted a meta-analysis on the associations between the FCGR2A H131R (rs1801274), FCGR3A F158V (rs...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-013-2599-y
更新日期：2013-08-01 00:00:00
abstract：:This meta-analysis was performed to assess the relationships between the PON1 Q192R (rs662 T>C) polymorphism and the clinical outcome of antiplatelet treatment after percutaneous coronary intervention (PCI). A range of electronic databases were searched: Web of Science (1945-2013), the Cochrane Library Database (Issue...
journal_title：Molecular biology reports
pub_type： 撤回出版物,收录出版
doi：10.1007/s11033-014-3509-7
更新日期：2014-09-01 00:00:00
abstract：:The aim of this study was to identify molecular markers to be applied to marker-assisted selection. Three SNPs of the FASN gene were studied. PCR-RFLP was used for genotyping. The SNPs g.17924A>G, g.17860C>T and g.15603A>G all in the FASN gene were genotyped using the enzymes MscI, DdeI and Hae III, respectively. The ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1883-6
更新日期：2012-12-01 00:00:00
abstract：:DNA repair capacity (DRC) can be altered based on sequence variations in DNA repair genes, which may result in cancer susceptibility. The current study was to evaluate the association between genetic polymorphisms, including associated haplotypes of xeroderma pigmentosum complementary group D (XPD), and individual sus...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0258-0
更新日期：2011-03-01 00:00:00
abstract：:Many case-control studies have investigated the role of TGF-β1 gene +869C/T promoter polymorphism in autoimmune diseases, but the results are inconsistent. To clarify this point, we performed a meta-analysis based on all available studies in Pubmed, Elsevier Science Direct, Google Searching, Chinese Biomedical Literat...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-013-2577-4
更新日期：2013-08-01 00:00:00
