Abstract:
:The binding of a cell nucleus stain, hematoxylin (HTL), to bovine serum albumin (BSA) was studied by spectroscopy including fluorescence spectra, UV-Visible absorption, circular dichroism (CD) spectra, synchronous and three-dimensional fluorescence spectra. The results indicated that the binding had led to static fluorescence quenching, with non-radiation energy transfer happening within single molecule. The observed binding constant was calculated to be 10(5.588) l mol(-1) at 311 K and one binding site had formed. The thermodynamic parameters of the interaction complied with DeltaG (theta) < 0, DeltaH (theta) < 0, DeltaS (theta) < 0 and the results indicate that hydrogen bonds played major role in the reaction. The distance r between donor (BSA) and acceptor (HTL) was obtained according to the Förster theory of non-radiation energy transfer. The structural change of BSA molecules with addition of HTL was analyzed and the optimized geometry of HTL-BSA was investigated by fluorescence probe method.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Zhang HX,Gao S,Xiong ZY,Liu SPdoi
10.1007/s11033-009-9448-zsubject
Has Abstractpub_date
2009-11-01 00:00:00pages
2299-306issue
8eissn
0301-4851issn
1573-4978journal_volume
36pub_type
杂志文章abstract::Identifying patients at high risk of metastasis is a major challenge in lung adenocarcinoma (ADC) therapy, therefore discovery of noninvasive biomarkers and therapeutic targets is urgent. We found significant differences between the secretomes of differentially expressed proteins in lung ADC cell lines, clinical tissu...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3641-4
更新日期:2014-11-01 00:00:00
abstract::Haemoglobin meseenger RNA was isolated from spleen erythroïd cells of anaemic rabbits. The mRNA fraction looks quite homogeneous when analyzed by sucrose gradient centrifugation; it sediments in the 9S region. When added to an ascites cell free system, spleen 9S RNA stimulates the incorporation of radioactive leucine ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00357405
更新日期:1973-06-01 00:00:00
abstract::Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05992-5
更新日期:2020-12-01 00:00:00
abstract::It has been suggested that the estrogen receptor alpha (ERα) and vitamin D receptor (VDR) genes as possibly implicated in reduced bone mineral density (BMD) in osteoporosis. The present study investigated the relation of ERα PvuII/XbaI polymorphisms and VDR FokI/TaqI polymorphisms with BMD in Turkish postmenopausal wo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1496-0
更新日期:2012-06-01 00:00:00
abstract::Insect molting is an important developmental process of metamorphosis, which is initiated by molting hormone. The molting process includes the activation of dermal cells, epidermal cells separation, molting fluid secretion, the formation of new epidermis and old epidermis excoriation etc. Polyphenol oxidases (PPOs), d...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2428-8
更新日期:2013-05-01 00:00:00
abstract::In the present study, Amukkara Chooram (AC) a well known herbal medicine was investigated for their antibiofilm efficacy against biofilm of Candida albicans. The biofilm inhibitory concentration of 20 µg/mL of AC showed promising effect by inhibiting the biofilm upto 60%. Morphogenic transition state of C. albicans fr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04757-z
更新日期:2019-06-01 00:00:00
abstract::To investigate whether Annexin 1 can protect a retinal ganglion cells line (RGC-5) from apoptosis as induced by serum deprivation. Annexin 1 location in RGC-5 cells was determined using an indirect immunofluorescent assay. Expression of Annexin 1 in RGC-5 cultures deprived of serum for 0, 2 days was semi-quantified by...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1358-1
更新日期:2012-05-01 00:00:00
abstract::The complete sequence of mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis) is determined by using PCR amplification, clone and primer-walking sequencing methods. The genome is 16836 bp in size, containing 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mtDNA genome of Siamensis Crocodile...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9062-x
更新日期:2008-06-01 00:00:00
abstract::Interleukin-13 (IL-13) is a TH2 cytokine with direct and indirect immunoregulatory functions on cancer cells. The cytokine has been reported to have some polymorphic variations at the gene level associated with some immune related diseases including asthma and allergy. In the present study, association of three IL13 g...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9400-7
更新日期:2009-09-01 00:00:00
abstract::In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via t...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0840-0
更新日期:2012-02-01 00:00:00
abstract::Familial Mediterranean fever is an autosomal recessive disorder characterized by recurrent attacks of abdominal pain, synovitis and pleuritis. MEFV gene mutations are responsible for the disease. The objective of this study was to identify the frequency and distribution of 12 MEFV mutations in 153 Syrian patients and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9475-9
更新日期:2010-01-01 00:00:00
abstract::C2H2 proteins belong to a group of transcription factors (TFs) existing as a superfamily that plays important roles in defense responses and various other physiological processes in plants. The present study aimed to screen for and identify C2H2 proteins associated with defense responses to abiotic and biotic stresses...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1542-y
更新日期:2012-06-01 00:00:00
abstract::BURP domain-containing genes comprise a large plant-specific family, yet the functions are very poorly understood, especially in maize (Zea mays) and sorghum (Sorghum vulgare). In this study, 26 BURP family genes in maize (ZmBURP01-15) and sorghum (SbBURP01-11) were identified including the gene structure, phylogeneti...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0587-z
更新日期:2011-10-01 00:00:00
abstract::In view of high mortality associated with coronary artery disease (CAD), development of an early predicting tool will be beneficial in reducing the burden of the disease. The database comprising demographic, conventional, folate/xenobiotic genetic risk factors of 648 subjects (364 cases of CAD and 284 healthy controls...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4236-2
更新日期:2018-10-01 00:00:00
abstract::Intrinsic bent DNA sites were identified in the 4289 bp segment encompassing the replication zone which directs DNA amplification and transcription of the C3-22 gene of Rhynchosciara americana. Restriction fragments showed reduced electrophoretic mobility in polyacrylamide gels. The 2D modeling of the 3D DNA path and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-006-0009-4
更新日期:2006-03-01 00:00:00
abstract::The Rv3203 (LipV) of Mycobacterium tuberculosis (Mtb) H37Rv, is annotated as a member of Lip family based on the presence of characteristic consensus esterase motif ‘GXSXG’. In vitro culture studies of Mtb H37Ra indicated that expression of Rv3203 gene was up-regulated during acidic stress as compared to normal wherea...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2861-3
更新日期:2014-01-01 00:00:00
abstract::Several lines of evidence suggest that the ubiquitin-proteasome pathway is involved in sepsis-induced muscle catabolism. The gene expression of ubiquitin and several of the proteasome subunits was increased in muscle from both septic rats and patients. In other studies, the activity of isolated 20S proteasomes was sti...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1006916206260
更新日期:1999-04-01 00:00:00
abstract::Altered expression and methylation pattern of tumor suppressor and DNA repair genes, in particular involved in mismatch repair (MMR) pathway, frequently occur in primary colorectal (CRC) tumors. However, little is known about (epi)genetic changes of these genes in precancerous and early stages of CRC. The aim of this ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2699-8
更新日期:2013-10-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) incidence is fast-growing especially in countries highly prevalent with viral hepatitis. Its poor prognosis has driven the research toward the discovery of sensitive markers for early detection. We investigated the usefulness of serum Transforming growth factor-beta1 (TGF-β1), Glypican-3...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2829-3
更新日期:2013-12-01 00:00:00
abstract::The Italian National Reference Center for Echinococcosis (CeNRE, Sassari, Italy) set up a diagnostic protocol of "one-step-PCR" useful for the detection of E. granulosus sensu stricto (E.g.s.s.) and the identification of its genotype (G1-G3). The purpose of this work was to perform the validation of the "PCR E.g.s.s."...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04625-w
更新日期:2019-04-01 00:00:00
abstract::Human Papillomavirus infection is considered as the main etiological factor of cervical cancer (ICC), although, the role of host genetic factors in ICC susceptibility has been increasing. Immunological response is crucial for the prevention of viral associated diseases. Interleukin 1 receptor antagonist (IL-1RN) is co...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1979-z
更新日期:2012-12-01 00:00:00
abstract::Knowledge in the area of genetic diversity could aid in providing useful information in the selection of material for breeding such as hybridization programs and quantitative trait loci mapping. To this end, 50 Nicotiana tabacum genotypes were genotyped with 21 primer combination of amplified fragment length polymorph...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3194-6
更新日期:2014-05-01 00:00:00
abstract::Among the bacteria groups, most of them are known to be beneficial to human being whereas only a minority is being recognized as harmful. The pathogenicity of bacteria is due, in part, to their rapid adaptation in the presence of selective pressures exerted by the human host. In addition, through their genomes, bacter...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-014-3367-3
更新日期:2014-08-01 00:00:00
abstract::Random amplified polymorphic DNA (RAPD) and microsatellite markers were applied to evaluate the genetic variation in endemic and endangered yellow catfish, Horabagrus brachysoma sampled from three geographic locations of Western Ghat, South India river systems. In RAPD, of 32 10-mer RAPD primers screened initially, 10...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9381-6
更新日期:2009-09-01 00:00:00
abstract::This experiment was conducted to explore the biological functions of myogenin (MyoG) gene. MyoG gene was cloned from genome of Hu sheep by overlap extension PCR. Then, pEGFP-C1-MyoG and pcDNA3.0-MyoG fusion expression vectors was constructed and pEGFP-C1-MyoG vector had been transfected into NIH-3T3 cells by liposomes...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2945-0
更新日期:2014-02-01 00:00:00
abstract::The difference in sensitivity of polyphenylalanine synthesis in extracts from Artemia salina and rabbit reticulocytes to inhibition by the Phytolacca americana protein (PAP) has been found to be linked to the source of the supernatant enzyme fraction and not the ribosomes. In the presence of reticulocyte supernatant e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00366257
更新日期:1976-04-01 00:00:00
abstract::Common bacterial blight (CBB), incited by Xanthomonas axonopodis pv. phaseoli (Xap), is a serious seed-borne disease of common bean (Phaseolus vulgaris L.) in both temperate and tropical production zones. The line HR45 is highly resistant to Xap infection on leaves and pods in both field and greenhouse. To understand ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0079-1
更新日期:2011-01-01 00:00:00
abstract::Scytovirin is a lectin isolated from the cyanobacterium Scytonema varium that has shown activity against HIV, SARS coronavirus and Zaire Ebola virus. Its 95 amino acids are divided into two structural domains (SD), the first spanning amino acids 1-48 (SD1) and the second 49-95 (SD2). Interestingly, the domains are nea...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-017-4116-1
更新日期:2017-08-01 00:00:00
abstract::Quinone oxidoreductases (NAD(P)H): quinone oxidoreductase 1 (NQO1) and NRH: quinone oxidoreductase 2 (NQO2) are an antioxidant enzyme, important in the detoxification of environmental carcinogens. Methylene-tetra-hydrofolate reductase (MTHFR), plays a role in folate metabolism and may have oncogenic role through disru...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2023-z
更新日期:2012-12-01 00:00:00
abstract::Acute lymphoblastic leukemia (ALL) is the most common pediatric malignancy. Genetic polymorphisms in the 3'UTR region of the CXCL12 (rs1801157) and TP53 codon 72 (rs1042522) genes may contribute to susceptibility to childhood ALL because they affect some important processes, such as metastasis regulation and tumor sup...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2551-1
更新日期:2013-07-01 00:00:00