Abstract：
:The Rv3203 (LipV) of Mycobacterium tuberculosis (Mtb) H37Rv, is annotated as a member of Lip family based on the presence of characteristic consensus esterase motif ‘GXSXG’. In vitro culture studies of Mtb H37Ra indicated that expression of Rv3203 gene was up-regulated during acidic stress as compared to normal whereas no expression was observed under nutrient and oxidative stress conditions. Therefore, detailed characterization of Rv3203 was done by gene cloning and its further expression and purification as his-tagged protein in microbial expression system. The enzyme was purified to homogeneity by affinity chromatography. It demonstrated broad substrate specificity and preferentially hydrolyzed p-nitrophenyl myristate. The purified enzyme demonstrated an optimum activity at pH 8.0 and temperature 50 °C. The specific activity, K m and V max of enzyme was determined to be 21.29 U mg(-1) protein, 714.28 μM and 62.5 μmol ml(-1) min(-1), respectively. The pH stability assay and circular dichroism spectroscopic analysis revealed that Rv3203 protein is more stable in acidic condition. Tetrahydrolipstatin, a specific lipase inhibitor and RHC80267, a diacylglycerol lipase inhibitor abolished the activity of this enzyme. The catalytic triad residues were determined to be Ser50, Asp180 and His203 residues by site-directed mutagenesis.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Singh G,Arya S,Narang D,Jadeja D,Singh G,Gupta UD,Singh K,Kaur Jdoi
10.1007/s11033-013-2861-3subject
Has Abstractpub_date
2014-01-01 00:00:00pages
285-96issue
1eissn
0301-4851issn
1573-4978journal_volume
41pub_type
杂志文章abstract：:Overexpression of domains of a human protein using recombinant DNA technology has been challenging because individual domains intend to accumulate as non-soluble aggregate when expressed separately. Studies on identifying right sequences for a domain to be able to fold independently may help understand the folding pat...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-016-3956-4
更新日期：2016-05-01 00:00:00
abstract：:Analysis of in vivo phosphorylation of mouse liver ribosomal proteins was performed by two-dimensional polyacrylamide gel electrophoresis following 32P-injection. Our method is special and differs from other eukaryotic systems reported in that all proteins separated on the first dimension gel are completely solubilize...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00356991
更新日期：1975-10-01 00:00:00
abstract：:Oxidative stress plays a major role in myocardial injury. Morin, a bioflavonoid has known to possess various biological activities in previous studies. Hence, this study evaluated the cardioprotective mechanism(s) of Morin against isoproterenol induced myocardial necrosis in rats. Male albino Wistar rats were divided ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-04575-9
更新日期：2019-02-01 00:00:00
abstract：:Insect molting is an important developmental process of metamorphosis, which is initiated by molting hormone. The molting process includes the activation of dermal cells, epidermal cells separation, molting fluid secretion, the formation of new epidermis and old epidermis excoriation etc. Polyphenol oxidases (PPOs), d...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2428-8
更新日期：2013-05-01 00:00:00
abstract：:In view of high mortality associated with coronary artery disease (CAD), development of an early predicting tool will be beneficial in reducing the burden of the disease. The database comprising demographic, conventional, folate/xenobiotic genetic risk factors of 648 subjects (364 cases of CAD and 284 healthy controls...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4236-2
更新日期：2018-10-01 00:00:00
abstract：:Biochemical networks comprise many diverse components and interactions between them. It has intracellular signaling, metabolic and gene regulatory pathways which are highly integrated and whose responses are elicited by extracellular actions. Previous modeling techniques mostly consider each pathway independently with...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2153-3
更新日期：2013-02-01 00:00:00
abstract：:The treatment of anaplastic astrocytoma (AA) is controversial. New chemotherapeutic approaches are needed for AA treatment. Temozolomide (TMZ) is one of the chemotherapeutic drugs for the treatment of AA. The cytotoxic effects of TMZ can be removed by the MGMT (O(6)-methylguanine-DNA methyltransferase) enzyme. Then, c...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2908-5
更新日期：2014-02-01 00:00:00
abstract：:Breast cancer is one of the most frequently diagnosed cancers and the leading cause of cancer deaths among females across the world, accounting for 23 % (1.38 million) of total new cancer cases and 14 % (0.45 million) of the total cancer deaths in 2008. c-kit is expressed in mast cell growth factor, cellular migration...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1786-6
更新日期：2012-09-01 00:00:00
abstract：:Emergence of MDR 'superbugs' inflamed a severe sense of urgency amongst scientists aiming at the discovery of novel potential drug molecules. Bacteria of the genus Streptomyces are really worth investigating for their immense potential to produce natural compounds of pharmaceutical importance. In the present study, th...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05731-w
更新日期：2020-09-01 00:00:00
abstract：:Recently, circulating miRNAs have been reported as promising biomarkers for various pathologic conditions including cancer. Certain microRNAs (miRNAs) have been shown early diagnostic potential for many types of cancer. The objective of this study was to investigate the potential of certain serum/plasma miRNAs as nove...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3322-3
更新日期：2014-07-01 00:00:00
abstract：:To investigate whether Annexin 1 can protect a retinal ganglion cells line (RGC-5) from apoptosis as induced by serum deprivation. Annexin 1 location in RGC-5 cells was determined using an indirect immunofluorescent assay. Expression of Annexin 1 in RGC-5 cultures deprived of serum for 0, 2 days was semi-quantified by...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1358-1
更新日期：2012-05-01 00:00:00
abstract：:It is well-established that an impaired adipose tissue function and morphology caused by a dysregulated gene expression contribute substantially to obesity. Nowadays, animal model studies and in vitro surveys provide evidence for possible roles of HDACs as emerging epigenetic players in the pathogenesis of obesity. Ho...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05431-5
更新日期：2020-05-01 00:00:00
abstract：:Large-scale random cDNA sequencing projects have been started for several organisms and are a valuable tool for the analysis of quantitative and qualitative aspects of gene expression. However, the reliability of the obtained data is limited as most of the clones are only partially analysed on one strand. As a consequ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00424432
更新日期：1996-01-01 00:00:00
abstract：:Published data on the association of vascular endothelial growth factor (VEGF) -1154G>A polymorphism with cancer risk is inconclusive. To derive a more precise estimation of association between VEGF -1154G>A polymorphism and the risk of cancer, we performed a meta-analysis of 7,071 cancer cases and 7,693 controls from...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-011-1326-9
更新日期：2012-05-01 00:00:00
abstract：:The binding characteristics of the interaction between 3-(2-cyanoethyl) cytosine (CECT) and human serum albumin (HSA) were investigated using fluorescence, UV absorption spectroscopic and molecular modeling techniques under simulative physiological conditions. The intrinsic fluorescence intensity of HSA was decreased ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1855-x
更新日期：2012-11-01 00:00:00
abstract：:The suppressors of cytokine signaling (SOCS) proteins are originally identified as negative regulators of cytokine-activated Janus kinase/signal transducers and activators of transcription signaling pathway, but increasing evidence reveals that SOCS proteins play an important role in the development of type 2 diabetes...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-014-3079-8
更新日期：2014-01-01 00:00:00
abstract：:Small ubiquitin-like modifier (SUMO) participates in post-translational modification of various target proteins. SUMOylation is an important molecular regulatory mechanism for plants to respond to abiotic stress. In the present study, GmSUMO2 gene was isolated from soybean seedlings for further study because of the hi...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05433-3
更新日期：2020-05-01 00:00:00
abstract：:In vivo and in vitro expressed N-terminal sequence of EWS (EAD) and hsRPB7 (subunit of human RNA polymerase II) were probed for protein-protein interactions using pull-down assays. In result, it was found that the proteins 57Z (residues 1-57 of EAD) and hsRPB7 interact in vitro forming a stable complex. The direct int...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9308-2
更新日期：2009-07-01 00:00:00
abstract：:Current study was conducted to investigation of effect of magnetic field on cell dedifferentiation and follow it callus induction derived from mature embryo culture in bread wheat genotypes. For this purpose, a factorial experiment based on completely randomized design was carried out with two wheat genotypes and thre...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2214-7
更新日期：2013-02-01 00:00:00
abstract：:This experiment was conducted to explore the biological functions of myogenin (MyoG) gene. MyoG gene was cloned from genome of Hu sheep by overlap extension PCR. Then, pEGFP-C1-MyoG and pcDNA3.0-MyoG fusion expression vectors was constructed and pEGFP-C1-MyoG vector had been transfected into NIH-3T3 cells by liposomes...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2945-0
更新日期：2014-02-01 00:00:00
abstract：:Killer cell immunoglobulin like receptor genes expressed by the natural killer cells and T cells of some subclasses are one of the very diversity and complex gene families on chromosome 19q13.4 which play key developmental role in the fight against viral infections, malignantly transformed cells and so on in the first...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4563-3
更新日期：2019-02-01 00:00:00
abstract：:The swine fatty acid binding protein gene (FABP3) has been screened with 4 primer sets for 270 purebred Yorkshire pigs from National Institute of Animal Science. The selection of primers was based on the swine cDNA sequence (AJ416019), and primer sets (FABP3-2 and FABP3-3) amplified target segments in real time PCR to...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0344-3
更新日期：2011-03-01 00:00:00
abstract：:Maize is one of the world's most important crops and a model for grass genome research. Long terminal repeat (LTR) retrotransposons comprise most of the maize genome; their ability to produce new copies makes them efficient high-throughput genetic markers. Inter-retrotransposon-amplified polymorphisms (IRAPs) were use...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05246-4
更新日期：2020-03-01 00:00:00
abstract：:Identifying patients at high risk of metastasis is a major challenge in lung adenocarcinoma (ADC) therapy, therefore discovery of noninvasive biomarkers and therapeutic targets is urgent. We found significant differences between the secretomes of differentially expressed proteins in lung ADC cell lines, clinical tissu...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3641-4
更新日期：2014-11-01 00:00:00
abstract：:The Italian National Reference Center for Echinococcosis (CeNRE, Sassari, Italy) set up a diagnostic protocol of "one-step-PCR" useful for the detection of E. granulosus sensu stricto (E.g.s.s.) and the identification of its genotype (G1-G3). The purpose of this work was to perform the validation of the "PCR E.g.s.s."...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04625-w
更新日期：2019-04-01 00:00:00
abstract：:Low levels of coenzyme Q10 (CoQ10) have been reported in the circulation of patients with breast cancer, particularly in metastatic features. Our objective was to study the correlation between plasma levels of CoQ10 and the tumoral expression levels of AMPK, PFKFB3, VEGF, and VEGFR2. This study was a part of consecuti...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05310-z
更新日期：2020-04-01 00:00:00
abstract：:BAP31 is a ubiquitously expressed integral membrane protein of the endoplasmic reticulum. BAP31 is involved in various biological and molecular processes, including protein transport, viral processing, apoptosis signaling, MHC 1 antigen processing and presentation, mitochondria and ER calcium regulation, and proteasom...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-020-06123-w
更新日期：2021-01-13 00:00:00
abstract：:This study was designed to identify the differential expression of the canonical transient receptor potential (TRPC) channels in the left ventricle of spontaneously hypertensive rats (SHR). Echocardiography studies were performed to compare the left ventricular function in SHR vs. Wistar-Kyoto rats (WKY), and the mRNA...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9792-z
更新日期：2010-07-01 00:00:00
abstract：:Current surgical reconstruction for soft tissue replacement involves lipotransfer to restore soft tissue replacements but is limited by survival and longevity of the fat tissue. Alternative approaches to overcome these limitations include using biodegradable scaffolds with stem cells with growth factors to generate so...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05297-7
更新日期：2020-03-01 00:00:00
abstract：:Drought is one of the major abiotic stresses that affect productivity in soybean (Glycine max L.) Several genes induced by drought stress include functional genes and regulatory transcription factors. The Arabidopsis thaliana DREB1D transcription factor driven by the constitutive and ABA-inducible promoters was introd...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3695-3
更新日期：2014-12-01 00:00:00
