Abstract:
:Analysis of in vivo phosphorylation of mouse liver ribosomal proteins was performed by two-dimensional polyacrylamide gel electrophoresis following 32P-injection. Our method is special and differs from other eukaryotic systems reported in that all proteins separated on the first dimension gel are completely solubilized, moving quantitatively to the second dimension gel. Only ribosomes from polysomes were used, ensuring analysis of ribosomes actively engaged in protein synthesis. We resolved sixty-five distinct proteins from ribosomes from membrane bound or free polysomes. In both cases radioautography revealed similar labeled patterns with one highly phosphorylated ribosomal protein and five marginally labeled spots.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Hoffman WL,Ilan Jdoi
10.1007/BF00356991keywords:
subject
Has Abstractpub_date
1975-10-01 00:00:00pages
219-24issue
3eissn
0301-4851issn
1573-4978journal_volume
2pub_type
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pub_type: 杂志文章
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