Abstract:
:Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional requirements during periods of reproductive activity. Therefore, a cDNA encoding Eriocheir sinensis FABP (Es-FABP9) was cloned based upon EST analysis of a testis cDNA library. The full length cDNA was 898 bp and encoded a 136 aa polypeptide that was highly homologous to related genes reported in shrimp. Gene expression analysis, as determined by RT-PCR, revealed the presence of Es-FABP9 transcripts was widely distributed with high and detectable expression levels observed in intestine, ovary, testis and heart, while expression were comparable among hepatopancreas, hemolymphe, gills, muscle, stomach and brain. Real-time quantitative RT-PCR analysis revealed that Es-FABP9 expression in testis, hemolymphe, hepatopancreas and ovarian was dependent on the status of testis development. Evidence provided in the present report supports a role of Es-FABP9 in lipid transport during the period of rapid testis growth in E. sinensis, and indirectly confirms the participation of the hepatopancreas, testis, hemolymphe and ovarian in lipid nutrient absorption and utilization processes.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Li WW,Jin XK,He L,Gong YN,Jiang H,Wang Qdoi
10.1007/s11033-010-0666-1subject
Has Abstractpub_date
2011-11-01 00:00:00pages
5169-77issue
8eissn
0301-4851issn
1573-4978journal_volume
38pub_type
杂志文章abstract::Caspases have been demonstrated to possess important functions in apoptosis and immune system in vertebrate. But there is less information reported on the oyster larval development. In the present work, two full-length molluscan caspase genes, named Cacaspase-2 and Cacaspase-3, were characterized for the first time fr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3833-y
更新日期:2015-05-01 00:00:00
abstract::PNAS-4 is a novel pro-apoptotic protein activated during the early response to DNA damage; however, the molecular mechanisms and pathways regulating PNAS-4 expression in tumors are not well understood. We hypothesized that PNAS-4 is a p53 down-stream target gene and designed this study. We searched online for putative...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0732-3
更新日期:2012-01-01 00:00:00
abstract::The complete sequence of mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis) is determined by using PCR amplification, clone and primer-walking sequencing methods. The genome is 16836 bp in size, containing 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mtDNA genome of Siamensis Crocodile...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9062-x
更新日期:2008-06-01 00:00:00
abstract::Recent Genome-Wide Association Studies have identified several single nucleotide polymorphisms (SNPs) associated with breast cancer (BC) among women of Asian, European, and African-American ancestry. Nevertheless, the contribution of these variants in the South American population is unknown. Furthermore, there is lit...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3236-0
更新日期:2014-06-01 00:00:00
abstract::Common bean is gaining acceptance as one of the most valuable major food consumed worldwide owing to innumerable nutritional and therapeutic benefits. Comparatively less productivity in underdeveloped countries encouraged us to proceed for QTL mining of yield traits in common bean. Heretofore, multiple yield associate...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05735-6
更新日期:2020-09-01 00:00:00
abstract::Selenium compounds have been widely investigated as novel anticancer agents due to high efficacy and selectivity against cancer cells in recent years. This study aimed to research the potential inhibitory effects of seleno-β-lactoglobulin (Se-β-Lg) on HepG2 cells in vitro. MTT results demonstrated that the synthetized...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04953-x
更新日期:2019-10-01 00:00:00
abstract::Changes in host immunity and parasite resistance to drugs are among the factors that contribute to decreased efficacy of antiparasitic drugs such as the antimonial compounds pentamidine, amphotericin (AMP B) and miltefosine. Bioactive natural products could be alternatives for the development of new drugs to treat neg...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05887-5
更新日期:2020-11-01 00:00:00
abstract::Using a membrane filter retention technique we have studied the interaction between DNA and lysine rich histone H5 in vitro. It is found that, depending on the ionic conditions, H5 can bind DNA in a random or cooperative manner and exhibits a preference to DNA with high molecular weight and/or high A + T content, as a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775749
更新日期:1980-03-31 00:00:00
abstract::Maize (Zea mays L.) is an important model plant with an important role in agriculture and national economies all over the world. The optimum growth temperature of maize is between 25 and 28 °C. At temperatures below 12 °C, maize is vulnerable to damage by chilling stress. MYB transcription factors play important roles...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04840-5
更新日期:2019-08-01 00:00:00
abstract::Although there are many studies on the role of PI3K/AKT/mTOR pathway and autophagy genes in the mechanism of head and neck cancer formation and prognostic significance, there is no study investigating the role of the genes in paranasal sinus carcinomas. The aim of the study was to assess the role of the PI3K/AKT/mTOR ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05458-8
更新日期:2020-05-01 00:00:00
abstract::Finger millet (Eleusine coracana L.) is an important crop used for food, forage, and industrial products. Three DNA marker techniques, random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and cytochrome P(450) gene based markers were used for the detection of genetic polymorphism in 83 accessions of f...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0067-5
更新日期:2010-12-01 00:00:00
abstract::The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and coding regions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Compar...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0431-5
更新日期:2011-04-01 00:00:00
abstract::Several lines of evidence suggest that the ubiquitin-proteasome pathway is involved in sepsis-induced muscle catabolism. The gene expression of ubiquitin and several of the proteasome subunits was increased in muscle from both septic rats and patients. In other studies, the activity of isolated 20S proteasomes was sti...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1006916206260
更新日期:1999-04-01 00:00:00
abstract::MAPK cascade is an important intracellular signaling module and function as a convergent point for crosstalk during abiotic stress signaling. In this study SbMAPKK gene has been isolated from Salicornia brachiata, a highly salt tolerant plant growing in costal marshes of Gujarat, India. The SbMAPKK gene is 1,023 bp lo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9774-1
更新日期:2010-02-01 00:00:00
abstract::The purpose of this study was to analyze the temporal gene expression in salivary and lacrimal glands of Sjögren's syndrome (SS) based on time-series microarray data. We downloaded gene expression data GSE15640 and GSE48139 from gene expression omnibus and identified differentially expressed genes (DEGs) at varying ti...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3471-4
更新日期:2014-09-01 00:00:00
abstract::A full-length cDNA encoding wheat peroxisomal ascorbate peroxidase (pAPX) was cloned by Suppression Subtractive Hybridization (SSH) and in silico approach. The cDNA was 1027 bp in length and contained a complete ORF of 876 bp, which encodes a protein of 292 amino acid residues. Its deduced amino acids sequence had 84%...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-005-4536-1
更新日期:2006-09-01 00:00:00
abstract::Free cytoplasmic mRNPs were isolated from human placenta. An activity of RNase was associated with these particles but was mostly inhibited by a labile protein inhibitor. Both RNase and RNase inhibitor were extractable from mRNPs by 0.5 M KCl. The nature of the association of the RNase-RNase inhibitor complex with mRN...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775355
更新日期:1984-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is considered as a long-term autoimmune disorder. Gene polymorphism and oxidative stress might be involved in the pathogenesis of the disease. We aimed to determine the association between PON-1L55M polymorphism and its effects on inflammatory markers such as anti-cytroline circulated-peptide...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4530-z
更新日期:2019-02-01 00:00:00
abstract::Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In the present study, we report the identification and characterization of a crustin (CrusSp) from the hemocyte of mud crab, Scylla paramamosain using an expressed sequence tag (EST) and rapid amplific...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9253-0
更新日期:2009-05-01 00:00:00
abstract::Late blight caused by Phytophthora infestans is the most important disease of potato. Many efforts have been made to understand molecular mechanism of the durable resistance to address the challenge raised by rapid evolution of the pathogen. A pathogenesis related protein (PR) gene StPRp27 was previously isolated from...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0937-5
更新日期:2012-02-01 00:00:00
abstract::A culture-independent molecular phylogenetic analysis was carried out to study for the first time the diversity of bacterial ammonia monooxygenase subunit A (amoA) and nitrogenase reductase subunit H (nifH) genes from Urca inlet at Guanabara Bay in Rio de Janeiro, Brazil. Most bacterial amoA and nifH sequences exhibit...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1111-9
更新日期:2012-04-01 00:00:00
abstract::One of the major limitations when attempting to obtain detailed biochemical, biophysical and immunological characterization of plant DNA mismatch repair proteins is their extremely low abundance in vivo under normal growth conditions. An initial analysis of PMS1 transcript level in various Arabidopsis thaliana tissues...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0203-2
更新日期:2011-02-01 00:00:00
abstract::The molecular composition of macromolecular assemblies engaged in transcription and splicing influences biogenesis of mRNA transcripts. Preference for one over the other interactive protein partner within those complexes is expected to change the gene expression pattern and to affect subsequent cellular events. We rep...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3128-3
更新日期:2014-01-01 00:00:00
abstract::BACs-on-Beads (BoBs™) assay is a rapid aneuploidy test (RAT) that detects numerical chromosomal aneuploidies and multiple microdeletion/microduplication syndromes. This study was conducted to appraise the usefulness of the BoB™ assay as a complementary diagnostic tool to conventional karyotyping for the rapid detectio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05117-7
更新日期:2020-01-01 00:00:00
abstract::Kidney transplantation to treat end-stage renal disease has evolved rapidly from the first successful transplantations to the current widespread use of grafts from both cadaveric and living donors. But acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate pos...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1321-1
更新日期:2012-05-01 00:00:00
abstract::Micro-RNAs (miRNAs) play key roles in regulating genes of the immune system. The aim of this study was to examine the expression of miR-1, 21 and 125a in the immune cells taken from the peripheral blood of patients suffering from chronic HBV infection (CHB). This cross-sectional study was performed on 60 CHB patients ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3355-7
更新日期:2014-07-01 00:00:00
abstract::Inhibitor of growth protein 3 (ING3), a new member of ING family, is involved in the regulation of various processes. In this study, a full-length cDNA of ING3 (named as RpING3) was cloned from the gill of Ruditapes philippinarum by rapid amplification of cDNA ends method for the first time. The cDNA obtained was 1442...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3221-7
更新日期:2014-06-01 00:00:00
abstract::A double-stranded RNA-specific nuclease (ds RNase) has been isolated and partially purified from human placenta by DEAE-cellulose and DNA-cellulose column chromatography. Denatured DNA-cellulose retained most of the single-stranded RNA-specific nuclease (ss RNase) activity, whereas the ds RNase came out in the void vo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775365
更新日期:1983-08-01 00:00:00
abstract::Proteasomes (prosomes) are large multiprotein complexes. They are involved in protein degradation of ubiquitin-conjugated proteins and in the generation of MHC class I peptides. We gave further evidence that they interfere with in vitro protein synthesis. Proteasomes inhibit the translation of Tobacco mosaic virus RNA...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00996354
更新日期:1994-01-01 00:00:00
abstract::3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors has been shown to reduce the progression of renal disease independent of cholesterol-lowering effect, but the mechanism of potential protective effect remains unclear. Here, we investigate the effect of fluvastatin on activation of nuclear factor-κB ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1264-6
更新日期:2012-04-01 00:00:00