PDIA3 mRNA expression and IL-2, IL-4, IL-6, and CRP levels of acute kidney allograft rejection in rat.

abstract：:A method is described which demonstrates the posibility of visualizing protein-complexed DNA by using pronase for both deproteinization and spreading of DNA for electron microscopy. The results show that proteolytic digestion is complete even under conditions of short formaldehyde fixation and pronase is an excellent ...

journal_title：Molecular biology reports

authors： Tsaneva I,Tsanev R

更新日期：1979-08-31 00:00:00

abstract：:Cell free protein synthesis has become a powerful method for the high-throughput production of proteins that are difficult to express in living cells. The protein SAP2 of Fasciola hepatica (FhSAP2), which has demonstrated to be both, an excellent vaccine candidate against experimental fascioliasis and a good antigen f...

journal_title：Molecular biology reports

authors： Ramos-Benítez MJ,Lopez-Cruz LM,Aguayo V,Ruiz-Jimenez C,Espino AM

更新日期：2018-10-01 00:00:00

abstract：:CD40 signaling plays a critical role in the survival rate of gastric cancer patients. Tumour samples were collected from 73 patients with who were diagnosed as gastric cancer in general surgery department in the 1st affiliated hospital of Suzhou University between September 2002 and July 2003. All patients had not rec...

journal_title：Molecular biology reports

authors： Li R,Chen WC,Pang XQ,Tian WY,Wang WP,Zhang XG

更新日期：2012-09-01 00:00:00

abstract：:Male Breast Cancer (MBC) is a rare and aggressive disease that is associated with genetic factors. Mutations in BRCA1 and BRCA2 account for 10% of all MBC cases suggesting that other genetic factors are involved. The aim of the present study is to screen whole BRCA1 and BRCA2 exons using the Ampliseq BRCA panel in Tun...

journal_title：Molecular biology reports

authors： Ben Kridis-Rejeb W,Ben Ayed-Guerfali D,Ammous-Boukhris N,Ayadi W,Kifagi C,Charfi S,Saguem I,Sellami-Boudawara T,Daoud J,Khanfir A,Mokdad-Gargouri R

更新日期：2020-09-01 00:00:00

abstract：:Rice production in recent years is highly affected by rapidly increasing temperatures in the tropical and sub-tropical countries, which threatens the sustainable production in near future. Hence, understanding the heat tolerance mechanism and evolving tolerant varieties is an immense need in the staple crop rice. An e...

journal_title：Molecular biology reports

authors： Wahab MMS,Akkareddy S,Shanthi P,Latha P

更新日期：2020-03-01 00:00:00

abstract：:Potato (Solanum tubersosum L.) is relatively vulnerable to abiotic stress conditions such as drought, but the tolerance mechanisms to such stress in potato are largely unknown. To gain insight into the transcriptome dynamics that are associated with drought stress, genome-wide gene expression profile was conducted by ...

journal_title：Molecular biology reports

authors： Zhang N,Liu B,Ma C,Zhang G,Chang J,Si H,Wang D

更新日期：2014-01-01 00:00:00

abstract：:Correct classification of genomic variants causing potentially aberrant splicing is of utmost importance for patient management, especially in clinically actionable genes such as BRCA1/2. In this article, we report molecular evaluation of the BRCA1 c.439T>C (rs794727800, p.Leu147=) variant based on RNA of a patient su...

journal_title：Molecular biology reports

authors： Minucci A,Mazzuccato G,D'Indinosante M,Di Nardo L,Concolino P,De Bonis M,Urbani A,Scambia G,Fagotti A,Capoluongo E

更新日期：2020-02-01 00:00:00

abstract：:Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats. We have mo...

journal_title：Molecular biology reports

authors： Pathak RU,Kanungo MS

更新日期：2007-03-01 00:00:00

abstract：:In jasmonate biosynthetic pathway, allene oxide synthase (AOS, EC 4.2.1.92), which is a cytochrome P450 (CYP74A), catalyzes the first committed step. We herein cloned a novel cDNA from Lonicera japonica Thunb., named LjAOS (GenBank accession: DQ303120), which was homologous to other AOSs. Southern blot analysis reveal...

journal_title：Molecular biology reports

authors： Jiang K,Pi Y,Hou R,Zeng H,Huang Z,Zhang Z,Sun X,Tang K

更新日期：2009-03-01 00:00:00

abstract：:Membrane-bound Δ9 desaturase perform oxygenated desaturation reactions to insert the first double bonds within fatty acyl chains between C9 and C10 positions of most saturated substrates. Arthrospira platensis, a blue green microalga, is an important source of polyunsaturated fatty acids (PUFA) such as oleic, linoleic...

journal_title：Molecular biology reports

authors： Ben Amor F,Ben Hlima H,Abdelkafi S,Fendri I

更新日期：2018-12-01 00:00:00

abstract：:The present study examined partial cytochrome b gene sequence of mitochondrial DNA for polymorphism and its suitability to determine the genetic differentiation in wild Labeo rohita. The 146 samples of L. rohita were collected from nine distant rivers; Satluj, Brahmaputra, Son, Chambal Mahanadi, Rapti, Chauka, Bhagira...

journal_title：Molecular biology reports

authors： Luhariya RK,Lal KK,Singh RK,Mohindra V,Punia P,Chauhan UK,Gupta A,Lakra WS

更新日期：2012-04-01 00:00:00

abstract：:The purpose of this research is to identify the probable mitochondrial factor associated with cytoplasmic male sterility (cms) by comparative analysis of cms and its isogenic maintainer lines in stem mustards. Dramatic variations in the morphology of floral organs were observed in cms stem mustard. Mitochondrial atpA ...

journal_title：Molecular biology reports

authors： Yang JH,Huai Y,Zhang MF

更新日期：2009-02-01 00:00:00

abstract：:70S RNA has been prepared from mouse mammary tumor virus (MMTV) produced by the GR tumor cell line. After denaturation for 3 min at 60 degrees C the RNA was applied to a sucrose gradient and molecules sedimenting between 10-16S were selected and passed over an oligo-dT cellulose column. The poly A containing RNA fract...

journal_title：Molecular biology reports

authors： Klemenz R,Reinhardt M,Diggelmann H

更新日期：1981-05-22 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy is a useful biophysical technique to study the ligand-protein interaction. In this report, we have used bacterially produced ERβ and its domains for studying the functional analysis of ligand-protein interaction. Briefly, ERβ and its transactivation domain (TAD) and ligand...

journal_title：Molecular biology reports

authors： Paramanik V,Thakur MK

更新日期：2011-10-01 00:00:00

abstract：:Molecular detection of Giardia duodenalis by polymerase chain reaction (PCR) is difficult in faecal samples due to inhibitors that contaminate DNA preparations, or due to low cyst concentrations. In order to eliminate inhibitors, improve cyst recovery and molecular detection of G. duodenalis, different types of water,...

journal_title：Molecular biology reports

authors： Bezagio RC,Colli CM,Romera LIL,de Almeida CR,Ferreira ÉC,Mattia S,Gomes ML

更新日期：2020-02-01 00:00:00

abstract：:To gain genetic insights into the protein-rich microalga, the transcriptome of Arthrospira platensis was sequenced using Illumina technology and de novo assembly was carried out. A total of 6023 transcripts were present in the transcriptome among which 4616 transcripts were annotated with specific functions. Gene onto...

journal_title：Molecular biology reports

authors： Kumaresan V,Sannasimuthu A,Arasu MV,Al-Dhabi NA,Arockiaraj J

更新日期：2018-10-01 00:00:00

abstract：:Latexin, a carboxypeptidase A inhibitor, is expressed in a cell type-specific manner in both central and peripheral nervous systems in the rat. It is used as a molecular marker for the regional specification of the neocortex. In this study, a cDNA was isolated from a human fetal brain cDNA library. The cDNA (LXN) cont...

journal_title：Molecular biology reports

authors： Liu Q,Yu L,Gao J,Fu Q,Zhang J,Zhang P,Chen J,Zhao S

更新日期：2000-01-01 00:00:00

abstract：:Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem cell (USSC) seems to be a suitable candidate. Recent studies have shown the involvement of microRNAs in se...

journal_title：Molecular biology reports

authors： Bakhshandeh B,Soleimani M,Hafizi M,Paylakhi SH,Ghaemi N

更新日期：2012-07-01 00:00:00

abstract：:Honey bee is important economic insect that not only pollinates fruits and crops but also provides products with various physiological activities. Bee venom is a functional agent that is widely applied in clinical treatment and pharmacy. Secapin is one of these agents that have a significant role in therapy. The funct...

journal_title：Molecular biology reports

authors： Hou C,Guo L,Lin J,You L,Wu W

更新日期：2014-12-01 00:00:00

abstract：:Cold stress adversely affects the growth and development of seedling of spring soybean. Revealing responses in seedling to cold stress at proteomic level will help us to breed cold-tolerant spring soybean cultivars. In this study, to understand the responses, a proteomic analysis on the leaves of seedlings of one cold...

journal_title：Molecular biology reports

authors： Tian X,Liu Y,Huang Z,Duan H,Tong J,He X,Gu W,Ma H,Xiao L

更新日期：2015-03-01 00:00:00

abstract：:Safe harbor loci allow predicable integration of a transgene into the genome without perturbing endogenous gene activity and for decades have been exploited in the mouse to investigate gene function, generate humanised models and create tissue specific reporter and Cre recombinase expressing lines. Herein, we show tha...

journal_title：Molecular biology reports

authors： Browning J,Rooney M,Hams E,Takahashi S,Mizuno S,Sugiyama F,Fallon PG,Kelly VP

更新日期：2020-02-01 00:00:00

abstract：:Chrysanthemum indicum L. is a traditional oriental medicinal herb prepared as a tea from flowers that have been used in China and South Korea since ancient times. It has a long history in the treatment of hypertension, inflammation, and respiratory diseases. Among Chrysanthemum species, C. indicum has more active chem...

journal_title：Molecular biology reports

authors： Abid S,Kaliraj L,Arif MH,Hurh J,Ahn JC,Yang DC,Jung SK

更新日期：2020-10-01 00:00:00

abstract：:Molecular DNA markers are used for detection of polymorphisms in individuals. As they are independent of developmental stage of the plant and environmental influences, they can be useful tools in taxonomy. The alleles of simple sequence repeat (SSR) markers (or microsatellites) are traditionally used to identify taxon...

journal_title：Molecular biology reports

authors： Tuler AC,Carrijo TT,Nóia LR,Ferreira A,Peixoto AL,da Silva Ferreira MF

更新日期：2015-11-01 00:00:00

abstract：:Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with othe...

journal_title：Molecular biology reports

authors： Amesz H,Haubrich T,Voorma HO

更新日期：1979-05-31 00:00:00

abstract：:Overexpression of domains of a human protein using recombinant DNA technology has been challenging because individual domains intend to accumulate as non-soluble aggregate when expressed separately. Studies on identifying right sequences for a domain to be able to fold independently may help understand the folding pat...

journal_title：Molecular biology reports

authors： Tiwary M,Agarwal N,Dinda A,Yadav SC

更新日期：2016-05-01 00:00:00

abstract：:The Giant Panda is an endangered and valuable gene pool in genetic, its important functional gene POLR2H encodes an essential shared peptide H of RNA polymerases. The genomic DNA and cDNA sequences were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) adopting touchdown-PCR and reve...

journal_title：Molecular biology reports

authors： Du YJ,Hou YL,Hou WR

更新日期：2013-02-01 00:00:00

abstract：:Covalent modification/demodification cycles are common in metabolism. When the modification and demodification steps are carried out by two independent enzymes, the degree of modification can be ultrasensitive to the total concentration of either catalyst. We recently showed that the degree of modification of a target...

journal_title：Molecular biology reports

authors： Ortega F,Ehrenberg M,Acerenza L,Westerhoff HV,Mas F,Cascante M

更新日期：2002-01-01 00:00:00

abstract：:The complete coding sequences of three porcine genes-SDHB, SNRPA and CRYBB1 were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the mouse or other mammals and highly homologous pig ESTs. These three genes were then deposited into GenBa...

journal_title：Molecular biology reports

authors： An QC,Liu GY

更新日期：2009-04-01 00:00:00

abstract：:Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induc...

journal_title：Molecular biology reports

authors： Wang WJ,Meng ZL,Mo YC,Liu JW,Sun CC,Hu SS,Zhang H

更新日期：2012-01-01 00:00:00

abstract：:Natural killer cell (NK) is known as a major immune system in body through mediating cell death via several possible pathways, and one of three subpopulations of lymphocytes functioning as scavenger of tumor, virus infected cells etc. Our present results found that the SOD-contained silkworm larvae powder caused an en...

journal_title：Molecular biology reports

authors： Yue WF,Yao ML,Liu JM,Li GL,Li XH,Wu XF,Deng W,Sun HX,Zhou JY,Zhang CX,Miao YG

更新日期：2009-01-01 00:00:00