Abstract：
:Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats. We have modified the technique to enhance reproducibility and reduce false positives and redundancy. It is fast and does not require any expensive or uncommon reagent. We choose to call it as subtractive differential display as it is a differential display performed over subtracted mRNA population. We have used this protocol successfully to clone a number of age-related differentially expressed sequences from rat brain that need to be sequenced to establish the gene identity.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Pathak RU,Kanungo MSdoi
10.1007/s11033-006-9010-1subject
Has Abstractpub_date
2007-03-01 00:00:00pages
41-6issue
1eissn
0301-4851issn
1573-4978journal_volume
34pub_type
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