当前位置： SCI文献检索 > MOLECULAR BIOLOGY REPORTS期刊下所有文献 > Improvement in cyst recovery and molecular detection of Giardia duodenalis from stool samples.

Improvement in cyst recovery and molecular detection of Giardia duodenalis from stool samples.

Abstract：

:Molecular detection of Giardia duodenalis by polymerase chain reaction (PCR) is difficult in faecal samples due to inhibitors that contaminate DNA preparations, or due to low cyst concentrations. In order to eliminate inhibitors, improve cyst recovery and molecular detection of G. duodenalis, different types of water, distillates (MDs), deionized (MDz), injection (MI) or Milli-Q® (MM) were used instead of formaldehyde (F) in the laboratory routine method (Ritchie). Cysts were isolated from faecal samples with low cyst concentrations (< 1 cyst/field), medium (1-2 cysts/field) or high (> 2 cysts/field). Cyst recovery was improved using all water types (MDs, MDz, MI, MM) compared to formaldehyde. At all cyst concentrations, the use of MM consistently showed the greatest recovery of G. duodenalis cysts . DNA samples from recovered cysts were tested for the glutamate dehydrogenase (GDH) and β-giardin (βg) genes. The use of Milli-Q® water allowed to detect both genes in all cyst concentrations, including low. The method processed with the other types of water amplified these genes at high and medium cyst concentrations. GDH and βg genes were not detected when the sample was processed with formaldehyde. These experimental results were confirmed in clinical samples. The results suggest that Milli-Q® water provides the highest cyst recovery from stool samples and, correspondingly, the highest sensitivity for detecting G. duodenalis by microscopy or PCR for GDH and βg genes, even at low concentration of cysts.

journal_name

Mol Biol Rep

journal_title

Molecular biology reports

authors

Bezagio RC,Colli CM,Romera LIL,de Almeida CR,Ferreira ÉC,Mattia S,Gomes ML

doi

10.1007/s11033-019-05224-5

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

1233-1239

issue

2

eissn

0301-4851

issn

1573-4978

pii

10.1007/s11033-019-05224-5

journal_volume

47

pub_type

杂志文章

相关文献

MOLECULAR BIOLOGY REPORTS文献大全
  • Candidate gene studies of diabetic retinopathy in human.

    abstract：:Diabetic retinopathy (DR) is a multifactorial disease with complex pathophysiology. It is the main cause of blindness among the people in productive age. The purpose of this literature review is to highlight recent achievements in the genetics of diabetic retinopathy with particular focus on candidate gene studies. We...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,评审

    doi：10.1007/s11033-016-4075-y

    authors： Priščáková P,Minárik G,Repiská V

    更新日期：2016-12-01 00:00:00

  • Effects of ischemic preconditioning on myocardium Caspase-3, SOCS-1, SOCS-3, TNF-α and IL-6 mRNA expression levels in myocardium IR rats.

    abstract：:The aim of this study was to characterise the effects of ischemic preconditioning (IP) on heart function parameters (ΔST and ΔT), activities of serum creatine kinase (CK), lactate dehydrogenase (LDH), and levels of serum nitric oxide (NO), malondialdehyde (MDA), and myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor nec...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-013-2677-1

    authors： Ma J,Qiao Z,Xu B

    更新日期：2013-10-01 00:00:00

  • Modeling response of glycolysis in S. cerevisiae cells harvested at diauxic shift.

    abstract：:The response of glycolysis to exposure of glucose in non-growing S. cerevisiae cells from diauxic shift was monitored. The result was compared to a kinetic model of glycolysis with branches to glycogen, trehalose, glycerol, and succinate. Experimental data at steady-state concentrations of metabolites in the upper par...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1023/a:1020349023158

    authors： Albers E,Bakker BM,Gustafsson L

    更新日期：2002-01-01 00:00:00

  • Expansion during PCR of short single-stranded DNA fragments carrying nonselfcomplementary dinucleotide or trinucleotide repeats.

    abstract：:We performed PCR of many DNA fragments of 6-32 nucleotides in length. Some of the fragments expanded into kilobase lengths even in the absence of the complementary strand. The dramatic expansion was observed for (CA)8, (TG)8, (CA)4, (CA)6, (CA)12, (TG)4, (TG)6, (TG)12, (TC)10, (GA)10 and other single strands. Similar ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1023/a:1024916602218

    authors： Reichová N,Kypr J

    更新日期：2003-09-01 00:00:00

  • Molecular and biochemical analysis of the beta class carbonic anhydrases in Caenorhabditis elegans.

    abstract：:The beta class of the carbonic anhydrase (CA) enzyme family has been found in plants, yeast, bacteria and algae, but not in animals. Also, little is known concerning the CAs of C. elegans. Genes possibly encoding beta-CAs were revealed by in silico analysis of the C. elegans genome. Amino acid sequence and 3D structur...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-009-9857-z

    authors： Fasseas MK,Tsikou D,Flemetakis E,Katinakis P

    更新日期：2010-07-01 00:00:00

  • Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina.

    abstract：:In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via t...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-0840-0

    authors： Meng Q,Xiao Z,Yuan H,Xue F,Zhu Y,Zhou X,Yang B,Sun J,Meng B,Sun X,Cheng F

    更新日期：2012-02-01 00:00:00

  • Sequence determination of the 3' end of mouse mammary tumor virus RNA.

    abstract：:70S RNA has been prepared from mouse mammary tumor virus (MMTV) produced by the GR tumor cell line. After denaturation for 3 min at 60 degrees C the RNA was applied to a sucrose gradient and molecules sedimenting between 10-16S were selected and passed over an oligo-dT cellulose column. The poly A containing RNA fract...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00778742

    authors： Klemenz R,Reinhardt M,Diggelmann H

    更新日期：1981-05-22 00:00:00

  • Molecular cloning of two molluscan caspases and gene functional analysis during Crassostrea angulata (Fujian oyster) larval metamorphosis.

    abstract：:Caspases have been demonstrated to possess important functions in apoptosis and immune system in vertebrate. But there is less information reported on the oyster larval development. In the present work, two full-length molluscan caspase genes, named Cacaspase-2 and Cacaspase-3, were characterized for the first time fr...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3833-y

    authors： Yang B,Li L,Pu F,You W,Huang H,Ke C

    更新日期：2015-05-01 00:00:00

  • Affinity purification of histidine-tagged proteins.

    abstract：:Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low ex...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF01674434

    authors： Schmitt J,Hess H,Stunnenberg HG

    更新日期：1993-10-01 00:00:00

  • Gain of function mutation in tobacco MADS box promoter switch on the expression of flowering class B genes converting sepals to petals.

    abstract：:One mutant transgenic line displaying homeotic conversion of sepals to petals with other phenotypic aberrations was selected and characterized at molecular level. The increased transcript level of gene encoding anthocyanidin synthase and petal specific class B genes, GLOBOSA and DEFECIENS in sepals of mutant line may ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-013-2909-4

    authors： Mahajan M,Yadav SK

    更新日期：2014-02-01 00:00:00

  • CTLA-4 exon 1 +49 polymorphism alone and in a haplotype with -318 promoter polymorphism may confer susceptibility to chronic HBV infection in Chinese Han patients.

    abstract：:Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) plays a pivotal role in regulating T cell activation, which is believably critical for the outcome of hepatitis B virus (HBV) infection. The expression and function of CTLA-4 may be affected by gene polymorphisms. This study investigated the influence of CTLA-4 poly...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0660-7

    authors： Duan S,Zhang G,Han Q,Li Z,Liu Z,Chen J,Lv Y,Li N,Wang Y,Li M,Lou S,Yang M,Zhu Q,Xing F

    更新日期：2011-11-01 00:00:00

  • Production of eicosapentaenoic acid (EPA, 20:5n-3) in transgenic peanut (Arachis hypogaea L.) through the alternative Δ8-desaturase pathway.

    abstract：:An important alternative source of fish oil is its production by plants through metabolic engineering. To produce eicosapentaenoic acid (EPA, 20:5n-3) in peanut through the alternative Δ8-pathway, a plant expression vector containing five heterologous genes driven by the constitutive 35S promoter respectively, namely,...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-018-4476-1

    authors： Wang C,Qing X,Yu M,Sun Q,Liu F,Qi B,Li X

    更新日期：2019-02-01 00:00:00

  • Study of the differential transcription in liver of growth hormone receptor (GHR), insulin-like growth factors (IGF1, IGF2) and insulin-like growth factor receptor (IGF1R) genes at different postnatal developmental ages in pig breeds.

    abstract：:The objective of this study was to determine hepatic expression levels of GHR, IGF1R, IGF1 and IGF2 genes in young growing gilts at different developmental ages (60-210 days) in five pig breeds: Polish Large White (PLW), Polish Landrace (PL), Pulawska (Pul), Duroc (Dur) and Pietrain (Pie). We studied the differences a...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-1068-8

    authors： Pierzchała M,Pareek CS,Urbański P,Goluch D,Kamyczek M,Różycki M,Smoczynski R,Horbańczuk JO,Kurył J

    更新日期：2012-03-01 00:00:00

  • Anomalous refractivity changes of gramicidin A in ethanol solutions.

    abstract：:Studies of the refractive properties of Gramicidin A in absolute ethanol and in ethanol water mixtures showed that this peptide in solution undergoes a conformational transition resulting in species with different refractivity. Accordingly, the folded form of this peptide shows a lower specific refractive index increm...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00357540

    authors： Derechin M,Jordan BE

    更新日期：1975-07-01 00:00:00

  • Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis.

    abstract：:This study was designed to examine the interactions of ergosterol with bovine serum albumin (BSA) and human serum albumin (HSA) under physiological conditions with the drug concentrations in the range of 2.99-105.88 μM and the concentration of proteins was fixed at 5.0 μM. The analysis of emission spectra quenching at...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1814-6

    authors： Cheng Z

    更新日期：2012-10-01 00:00:00

  • In-vitro and in-silico investigation of protective mechanisms of crocin against E46K α-synuclein amyloid formation.

    abstract：:α-Synuclein is a presynaptic neuronal protein that is abundant in the human brain and is linked genetically and neuropathologically to Parkinson's disease (PD). The E46K mutation of the α-synuclein gene has been linked to autosomal dominant early-onset of PD. Crocin is a carotenoid chemical compound of saffron that ha...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04882-9

    authors： Ghasemi Tigan M,Ghahghaei A,Lagzian M

    更新日期：2019-08-01 00:00:00

  • UV crosslinking of RNA to nylon membrane enhances hybridization signals.

    abstract：:An improvement in the detection by nucleic acid hybridization of size-fractionated RNA immobilized to nylon-based membranes is described. Electrophoretic transfer of RNA to nylon membranes permits a quantitative determination of different RNA transcripts on the same membrane after sequential hybridization using differ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00364822

    authors： Khandjian EW

    更新日期：1986-01-01 00:00:00

  • miR-143 expression profiles in urinary bladder cancer: correlation with clinical and epidemiological parameters.

    abstract：:Hsa-mir-143 and hsa-let-7c have been reported to be deregulated in multiple neoplasms. The main purpose of this study was to investigate the expression of these miRNAs in bladder cancer (BCa) and to analyze the association between their expression profiles and clinical and epidemiological parameters. Ninety BCa specim...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-05228-1

    authors： Boubaker NS,Spagnuolo M,Trabelsi N,Said R,Gurtner A,Regazzo G,Ayed H,Blel A,Karray O,Saadi A,Rammeh S,Chebil M,Rizzo MG,Piaggio G,Ouerhani S

    更新日期：2020-02-01 00:00:00

  • Dysregulated lipid metabolism in hepatocellular carcinoma cancer stem cells.

    abstract：:According to the stem cell theory for cancer, hepatocellular carcinomas are sustained by a group of cancer stem cells (CSCs) which are responsible for resistance to chemotherapy. In the present study we aimed to examine lipid metabolism in cancer stem cells induced by long-term treatment with sorafenib and its relatio...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-020-05352-3

    authors： Bort A,Sánchez BG,de Miguel I,Mateos-Gómez PA,Diaz-Laviada I

    更新日期：2020-04-01 00:00:00

  • De novo assembly of genome and development of polymorphic microsatellite loci in the blue swimming crab (Portunus pelagicus) using RAD approach.

    abstract：:The blue swimming crab (Portunus pelagicus) is a valuable marine fishery resource in Indo-West Pacific Ocean. So far, rare genetic resource of this species is available. In this report, the restriction-site associated DNA (RAD) approach was employed to mine the genomic information and identify molecular markers in P. ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-018-4339-9

    authors： Wu Q,Miao G,Li X,Liu W,Ikhwanuddin M,Ma H

    更新日期：2018-12-01 00:00:00

  • Molecular cloning and characterization of crustin from mud crab Scylla paramamosain.

    abstract：:Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In the present study, we report the identification and characterization of a crustin (CrusSp) from the hemocyte of mud crab, Scylla paramamosain using an expressed sequence tag (EST) and rapid amplific...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-008-9253-0

    authors： Imjongjirak C,Amparyup P,Tassanakajon A,Sittipraneed S

    更新日期：2009-05-01 00:00:00

  • Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line.

    abstract：:The aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hINS) expression in transgenic buffalo for therapeutic use. We have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitr...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3464-3

    authors： Kaushik R,Singh KP,Kumari A,Rameshbabu K,Singh MK,Manik RS,Palta P,Singla SK,Chauhan MS

    更新日期：2014-09-01 00:00:00

  • Identification of the tree shrew ATP-binding cassette transporter A1 (ABCA1) and its expression in tissues : cDNA sequence and expression of tree shrew ABCA1.

    abstract：:ATP-binding cassette transporter A1 (ABCA1) modulates plasma levels of high density lipoprotein (HDL), a cardiovascular protecting factor. Tree shrew was considered to be an animal protected from atherosclerosis characterized by high proportion of HDL in plasma. The cDNA clones and expression of tree shrew ABCA1 was i...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-007-9199-7

    authors： Zeng WW,Zhou B,Liu HR,Sun GT,Chen BS

    更新日期：2009-03-01 00:00:00

  • 3-Deoxyglucosone interferes with insulin signaling and attenuates insulin action on glucose-induced GLP-1 secretion in the enteroendocrine L cell line STC-1.

    abstract：:Maintenance of glucose homeostasis is reciprocally regulated by insulin and glucagon-like peptide-1 (GLP-1). We previously reported that GLP-1 secretion in response to an oral glucose load was impaired following an administration of 3-deoxyglucosone (3DG), an independent factor associated with the development of pre-d...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04926-0

    authors： Song X,Zhou L,Xu H,Wang F,Liang G,Zhang L,Huang F,Jiang G

    更新日期：2019-10-01 00:00:00

  • Aragonite shells are more ancient than calcite ones in bivalves: new evidence based on omics.

    abstract：:Two calcium carbonate crystal polymorphs, aragonite and calcite, are the main inorganic components of mollusk shells. Some fossil evidences suggest that aragonite shell is more ancient than calcite shell for the Bivalvia. But, the molecular biology evidence for the above deduction is absent. In this study, we searched...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3620-9

    authors： Wang X,Li L,Zhu Y,Song X,Fang X,Huang R,Que H,Zhang G

    更新日期：2014-11-01 00:00:00

  • Toll-like receptors 4 induces expression of matrix metalloproteinase-9 in human aortic smooth muscle cells.

    abstract：:Recent evidence supports a role of Toll-like receptor (TLR) signaling in the development of atherosclerotic lesions. It was confirmed that the presence of functional TLR4 promotes a proinflammatory phenotype and proliferation of vascular smooth muscle cells (VSMCs). Here we tested whether designed TLR4 small interferi...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0246-4

    authors： Li H,Xu H,Liu S

    更新日期：2011-02-01 00:00:00

  • 1-Deoxynojirimycin modulates glucose homeostasis by regulating the combination of IR-GlUT4 and ADIPO-GLUT4 pathways in 3T3-L1 adipocytes.

    abstract：:1-Deoxynojirimycin (DNJ), the main alkaloid in mulberry leaves, was recognized to treat patients with type 2 diabetes mellitus (T2DM). However, the regulatory mechanism of DNJ on glucose homeostasis was still unclear. In the present study, a safe concentration of 0.1-10 μmol/L for DNJ was incubated with mature 3T3-L1 ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-05069-y

    authors： Li Q,Wang Y,Dai Y,Shen W,Liao S,Zou Y

    更新日期：2019-12-01 00:00:00

  • Mitochondrial dynamic changes in health and genetic diseases.

    abstract：:Mitochondria are highly specialized in function, but mitochondrial and, therefore, cellular integrity is maintained through their dynamic nature. Through the frequent processes of fusion and fission, mitochondria continuously change in shape and adjust function to meet cellular requirements. Abnormalities in fusion/fi...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,评审

    doi：10.1007/s11033-014-3663-y

    authors： Chen L,Winger AJ,Knowlton AA

    更新日期：2014-11-01 00:00:00

  • Role of Spata34 in cell proliferation and its expression pattern in postnatal development of rat testis.

    abstract：:Spata34 is a testis-specific-expressed gene which exerts diverse functions in testis development. This study intends to examine the expression profiles of Spata34 in postnatal rat testis, and explore its potential roles in cell proliferation in vitro. We found that the mRNA and protein expression levels of Spata34 wer...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-018-4439-6

    authors： Nie D,Li H,Liu Y,Liao Z

    更新日期：2018-12-01 00:00:00

  • rs2651899 variant is associated with risk for migraine without aura from North Indian population.

    abstract：:Recently a GWAS study had identified 38 genomic variants commonly found in humans that influence migraine risk. For further replicate these findings, we selected two SNPs; rs2651899 on chromosome 1p36.32 in PRDM16 gene and rs10166942 on chromosome 2q37.1 close to TRPM8 gene for their associations with migraine in the ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04593-1

    authors： Kaur S,Ali A,Ahmad U,Pandey AK,Singh B

    更新日期：2019-02-01 00:00:00