Abstract：
:Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni(2+)-NTA chromatography.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Schmitt J,Hess H,Stunnenberg HGdoi
10.1007/BF01674434subject
Has Abstractpub_date
1993-10-01 00:00:00pages
223-30issue
3eissn
0301-4851issn
1573-4978journal_volume
18pub_type
杂志文章abstract：:Glutamate represents the main excitatory neurotransmitter in the mammalian brain; however, its excessive elevation in the extracellular space is cytotoxic and can result in neuronal death. The ischemia initiated brain damage reflects changes in glutamate concentration in peripheral blood. This paper investigated the r...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05626-w
更新日期：2020-07-01 00:00:00
abstract：:The novel dipstick DNA extraction method was tested for its reliability and usability for biological barcoding in comparison to a commercial kit and to a simplified isopropanol precipitation method using crayfish gill tissue. Following DNA extraction, the mitochondrial COI-gene was amplified in a PCR-reaction using a ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05083-0
更新日期：2019-12-01 00:00:00
abstract：:We investigated phenotypic variations for pod shattering, pod length and number of seeds per pod in large germplasm collections of Brassica juncea (2n = 36; AABB) and its progenitor species, B. rapa (2n = 20; AA) and B. nigra (2n = 16; BB). Pod shatter resistance was measured as energy required for rupturing a mature ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05384-9
更新日期：2020-04-01 00:00:00
abstract：:C2H2 proteins belong to a group of transcription factors (TFs) existing as a superfamily that plays important roles in defense responses and various other physiological processes in plants. The present study aimed to screen for and identify C2H2 proteins associated with defense responses to abiotic and biotic stresses...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1542-y
更新日期：2012-06-01 00:00:00
abstract：:Northeast of China is the main soybean production area, drought and low-temperature tolerance are both main factors involved in reducing soybean yield and limiting planting regions, the most effective way to solve this problem is to breed cultivars with drought and low-temperature tolerance. A set of the BC(2)F(3) lin...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1423-9
更新日期：2012-05-01 00:00:00
abstract：:The lysyl oxidase-like 2 (LOXL2) protein is a human paralogue of lysyl oxidase (LOX) that functions as an amine oxidase for formation of lysine-derived cross-links found in collagen and elastin. In addition to the C-terminal domains characteristic to the LOX family members, LOXL2 contains four scavenger receptor cyste...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0088-0
更新日期：2011-01-01 00:00:00
abstract：:Myocardial ischemia and reperfusion (MI/R) is associated with an intense inflammatory reaction, which may lead to myocyte injury. In this study, we investigated the effect of quercetin, an inhibitor of c-Jun N-terminal kinase on ischemia/reperfusion injury in isolated rat hearts. Rat models of MI/R were induced by cor...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2002-4
更新日期：2012-12-01 00:00:00
abstract：:Selenium compounds have been widely investigated as novel anticancer agents due to high efficacy and selectivity against cancer cells in recent years. This study aimed to research the potential inhibitory effects of seleno-β-lactoglobulin (Se-β-Lg) on HepG2 cells in vitro. MTT results demonstrated that the synthetized...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04953-x
更新日期：2019-10-01 00:00:00
abstract：:Abnormally protein aggregation and deposition are key pathological features of ALS, which may related with dysfunctional cellular autophagy. In the current study, we found that, compared with wtSOD1 cells, serum starvation treatment resulted in significant higher percentage of apoptosis in mutSOD1 cells; Lithium treat...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05071-4
更新日期：2019-12-01 00:00:00
abstract：:P73 is a structural and functional homologue of p53, and plays an important role in regulating cell cycle and apoptosis. A potentially functional polymorphism (designated as p73 G4C14-to-A4T14) has been identified in a region in exon 2 of the p73 gene, which may theoretically form a stem-loop structure and thereby aff...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-011-0913-0
更新日期：2012-02-01 00:00:00
abstract：:Long non-coding RNAs (LncRNAs) are non-coding RNAs. The potential roles of lncRNAs in type 2 diabetes mellitus (T2DM) are not well-known. In this study, we aim to assess the expression levels of LY86-AS1 and HCG27_201 in T2DM patients and a healthy control group. We obtained whole blood and serum samples from 100 T2DM...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4429-8
更新日期：2018-12-01 00:00:00
abstract：:The recombinant catalytic subunit of human protein kinase CK2 bas been mutagenised at the C-terminal region in an attempt to induce this tail to fold. We suppose in fact that this unstructured C-terminus just might be responsible for the high degradability of the human enzyme. On the basis of theoretical calculations ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1023934805326
更新日期：2003-06-01 00:00:00
abstract：:The melanocortin-4 receptor (MC4R) plays an important role in weight and energy homeostasis and it is associated with lower risk to develop obesity and lower body mass index. The contribution of MC4R mutation to obesity in Vojvodina (Northern Province of Serbia), known as a region with the largest number of overweight...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9500-z
更新日期：2010-01-01 00:00:00
abstract：:Complexity is one of the hallmarks that applies to C2H2 type zinc finger proteins (ZFPs). Structurally distinct clusters of zinc finger modules define an extremely large superfamily of nucleic acid binding proteins with several hundred, perhaps thousands of different members in vertebrates. Recent discoveries have pro...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/BF00999848
更新日期：1994-07-01 00:00:00
abstract：:Schizophrenia is one of the neuropathological disorders, which are associated with dopamine and its receptors. In recent years, it has been shown that mRNA of D3, D4 and D5 dopamine receptor (DRD3, DRD4, DRD5) subtypes is expressed in human peripheral blood lymphocytes (PBL). A total 55 schizophrenic patients and 51 h...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0396-4
更新日期：2011-04-01 00:00:00
abstract：:MAPK kinase 1 interacting protein 1 (MAP2K1ip1) is an important scaffold proteins of the mitogen-activated protein kinase (MAPK) pathway that form an active signaling module and enhance the specificity and spatiality of MAPK signaling. In the present study, we identified and characterized a MAP2K1ip1 cDNA from tiger s...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1391-0
更新日期：2012-05-01 00:00:00
abstract：:Prkar1b gene encodes regulatory type I, beta subunit (RIβ) of cAMP dependent protein kinase A in mouse. Among the various isoforms of regulatory and catalytic subunits that comprise mammalian PKA, RIβ subunit is considered to be one of the important subunits for neuronal functions. This is involved in multiple forms o...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1108-4
更新日期：2012-04-01 00:00:00
abstract：:We reported previously that TspGWI, a prototype enzyme of a new Thermus sp. family of restriction endonucleases-methyltransferases (REases-MTases), undergoes the novel phenomenon of sinefungin (SIN)-caused specificity transition. Here we investigated mutant TspGWI N473A, containing a single amino acid (aa) substitutio...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3085-x
更新日期：2014-01-01 00:00:00
abstract：:Cartilage has a limited self-repair capability and the repair of large cartilage defects remains a challenge in clinic. This study aimed to investigate the effect of mesenchymal stem cells (MSCs) loaded three-dimensional bilayer collagen scaffold for cartilage repair. Cross-linked three-dimensional bilayer collagen sc...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0853-8
更新日期：2012-02-01 00:00:00
abstract：:Geranylgeranyl diphosphate synthase (GGPPS) [EC 2.5.1.29] catalyzes the biosynthesis of geranylgeranyl diphosphate (GGPP), which is a key precursor for diterpenes such as taxol. Herein, a full-length cDNA encoding GGPPS (designated as CgGGPPS) was cloned and characterized from hazel (Corylus avellana L. Gasaway), a ta...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9935-2
更新日期：2010-10-01 00:00:00
abstract：:We have previously shown that angiotensin-converting enzyme (ACE) gene D allele is an independent risk factor for early onset coronary artery disease (CAD). Little is known about the concomitant presence of the ACE gene D allele and paraoxonase (PON1) codon 192 arginine (Arg) on the severity of CAD. Regarding the high...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1027-4
更新日期：2012-03-01 00:00:00
abstract：:Methicillin resistant Staphylococcus aureus (MRSA) is one of the challenging bacterial pathogen due to its acquired resistance to the β lactam antibiotics. The Sortase A is an enzyme of Gram-positive bacteria including S. aureus to anchor surface proteins to the cell wall. Sortase A is well studied enzyme and consider...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3384-2
更新日期：2014-08-01 00:00:00
abstract：:Previous studies from our group have brought forward the concept of angiogenic regeneration during radiotherapy (RT), as a major cause of RT failure. This process was examined herein in rectal cancer patients undergoing preoperative chemo-radiotherapy. Out of 25 patients with stage II/III rectal adenocarcinoma, 15 had...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04668-z
更新日期：2019-04-01 00:00:00
abstract：:According to the stem cell theory for cancer, hepatocellular carcinomas are sustained by a group of cancer stem cells (CSCs) which are responsible for resistance to chemotherapy. In the present study we aimed to examine lipid metabolism in cancer stem cells induced by long-term treatment with sorafenib and its relatio...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05352-3
更新日期：2020-04-01 00:00:00
abstract：:Chrysanthemum indicum L. is a traditional oriental medicinal herb prepared as a tea from flowers that have been used in China and South Korea since ancient times. It has a long history in the treatment of hypertension, inflammation, and respiratory diseases. Among Chrysanthemum species, C. indicum has more active chem...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05844-2
更新日期：2020-10-01 00:00:00
abstract：:The goal of this study was to determine whether ultrasound (US) exposure combined with microbubble destruction could be used to enhance non-viral gene delivery in rat C6 glioma cells. Microbubbles were prepared and gently mixed with plasmid DNA. The mixture of the DNA and microbubbles was administered to cultured C6 c...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9307-3
更新日期：2009-07-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) incidence is fast-growing especially in countries highly prevalent with viral hepatitis. Its poor prognosis has driven the research toward the discovery of sensitive markers for early detection. We investigated the usefulness of serum Transforming growth factor-beta1 (TGF-β1), Glypican-3...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2829-3
更新日期：2013-12-01 00:00:00
abstract：:Tumor microenvironment (TME) crucially functions in tumor initiation and progression. Stroma-tumor interactions and cellular transdifferentiation are the prerequisite for tumor formation. Transforming growth factor-β (TGF-β), a major cytokine secreted by tumor-associated fibroblasts (TAFs) and cancer cells, is a cruci...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04719-5
更新日期：2019-06-01 00:00:00
abstract：:This study is to explore the relationship between vascular endothelial growth factor (VEGF) and pathological changes in cryptorchidism by using murine model of intraperitoneal cryptorchidism with surgical operation. To investigate the relationship between the changes of VEGF expression and heat stress inducing germ ce...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05454-y
更新日期：2020-05-01 00:00:00
abstract：:The Pro12Ala and C161T polymorphisms in peroxisome proliferator-activated receptor γ (PPARγ) have been shown to be associated with carotid artery atherosclerosis. It remains unclear whether these two polymorphisms are associated with risk factors for cardiovascular disease (CVD) in hemodialysis (HD) patients. Therefor...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3645-0
更新日期：2014-11-01 00:00:00
