Expression, purification and characterisation of a novel mutant of the human protein kinase CK2.

abstract：:Abnormally protein aggregation and deposition are key pathological features of ALS, which may related with dysfunctional cellular autophagy. In the current study, we found that, compared with wtSOD1 cells, serum starvation treatment resulted in significant higher percentage of apoptosis in mutSOD1 cells; Lithium treat...

journal_title：Molecular biology reports

authors： Yin X,Wang S,Wang X,Yang Y,Jiang H,Wang T,Wang Y,Zhang C,Feng H

更新日期：2019-12-01 00:00:00

abstract：:Biochemical networks comprise many diverse components and interactions between them. It has intracellular signaling, metabolic and gene regulatory pathways which are highly integrated and whose responses are elicited by extracellular actions. Previous modeling techniques mostly consider each pathway independently with...

journal_title：Molecular biology reports

authors： Tomar N,Choudhury O,Chakrabarty A,De RK

更新日期：2013-02-01 00:00:00

abstract：:Previous report showed that Estrogen related receptor α (ERRα) knockout mice had a significant reduction in adipose tissue deposition. Although it was reported that ERRα could promote adipogenesis in several immortalized preadipocytes cell lines, the mechanism behind which is still unclear to date. Besides, the expres...

journal_title：Molecular biology reports

authors： Ju D,He J,Zhao L,Zheng X,Yang G

更新日期：2012-03-01 00:00:00

abstract：:One of the most crucial steps for preventing viral pandemics is the early detection of the causative virus on site. Various molecular and immunological approaches have been developed for virus detection. In this study, we investigated the utility of the recently introduced convection polymerase chain reaction (cPCR) p...

journal_title：Molecular biology reports

authors： Lee MH,Song KY,Hwang HJ,Kim JH,Hwang I

更新日期：2019-10-01 00:00:00

abstract：:Adipose differentiation-related protein gene (ADP) plays an important role in controlling lipid accumulation in mammals. It may also affect lipid deposition in birds. However, the molecular mechanism of its actions in birds remains unknown. In the present study, the coding sequence of ADP cDNA for Chinese native breed...

journal_title：Molecular biology reports

authors： Zhao X,Zhu Q,Wang Y,Yang Z,Liu Y

更新日期：2010-07-01 00:00:00

abstract：:p53 helps in maintaining genomic stability by undergoing cellular arrest, DNA repair or cellular apoptosis during DNA damage. So, as to find the association of p53Arg 72 Pro towards lung carcinogenesis and overall survival of North Indian lung cancer patients, single nucleotide polymorphic variant (rs1042522) was anal...

journal_title：Molecular biology reports

authors： Kumari A,Bahl C,Singh N,Behera D,Sharma S

更新日期：2016-12-01 00:00:00

abstract：:Based on the internal transcribed spacer and inter-simple sequence repeats (ISSR), the phylogenetic relationship and genetic diversity of Metarhizium spp., pathogens found in Chinese burrower bugs, Schiodtella formosana, were analyzed. The results showed that the causative agents of the epizootic green muscardine dise...

journal_title：Molecular biology reports

authors： Luan F,Zhang S,Wang B,Huang B,Li Z

更新日期：2013-01-01 00:00:00

abstract：:Cotton fiber cells elongate without dividing to form economically valuable spinnable fiber. Reports of the ploidy level of fiber cells are variable. Early reports indicated an increase in nuclear DNA content in young fibers; however, subsequent reports failed to observe such a significant increase in ploidy level. Eva...

journal_title：Molecular biology reports

authors： Taliercio E,Ray J,Scheffler J

更新日期：2010-02-01 00:00:00

abstract：:The mechanism by which external Bax releases cytochrome c is still controversial and may also depend on the type of mitochondria and the actual localisation of cytochrome c. Outer membrane porin acquires high binding affinity for hexokinase by interacting with the adenine nucleotide translocator (ANT) in the contact s...

journal_title：Molecular biology reports

authors： Vyssokikh MY,Zorova L,Zorov D,Heimlich G,Jürgensmeier JJ,Brdiczka D

更新日期：2002-01-01 00:00:00

abstract：:As a kind of binding protein, the type 1 Na(+)/H(+) exchanger (NHE1) is a receptor for the highly pathogenic Avian leukosis viruses-J subgroup (ALV-J) in chicken. In order to investigate the potential effect of chicken NHE1 gene on leukosis, we compared its expression between ALV-J-affected and -unaffected chicken, sc...

journal_title：Molecular biology reports

authors： Chen B,Pan W,Zhang L,Liu J,Ouyang H,Nie Q,Zhang X

更新日期：2014-10-01 00:00:00

abstract：:Overexpression of differentiated embryo chondrocyte 1 (DEC1) has been reported to contribute to the cellular differentiation, proliferation, and apoptosis of various cancers. Our previous studies have shown that DEC1 was highly expressed in gastric cancer (GCa) tissues. However, there is no report about the expression...

journal_title：Molecular biology reports

authors： Zheng Y,Shi X,Wang M,Jia Y,Li B,Zhang Y,Liu Q,Wang Y

更新日期：2012-04-01 00:00:00

abstract：:Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studi...

journal_title：Molecular biology reports

authors： Rodríguez-Hoces de la Guardia A,Ugalde MB,Lobos-Diaz V,Romero-Romero JL,Meyer-Regueiro C,Inostroza-Blancheteau C,Reyes-Diaz M,Aquea F,Arce-Johnson P

更新日期：2021-01-27 00:00:00

abstract：:High glycemic index diet can induce multiple diseases. Many research indicated that oxidative stress played important role in many pathological conditions. However, the impact of gene expression and dietary habit on oxidation process are still less clear. We used high-glucose diet to feed C57BL/6J mice for 4 weeks, me...

journal_title：Molecular biology reports

authors： Du D,Shi YH,Le GW

更新日期：2010-12-01 00:00:00

abstract：:In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via t...

journal_title：Molecular biology reports

authors： Meng Q,Xiao Z,Yuan H,Xue F,Zhu Y,Zhou X,Yang B,Sun J,Meng B,Sun X,Cheng F

更新日期：2012-02-01 00:00:00

abstract：:We report complete mitochondrial genome of Northern Indian red muntjac, Muntiacus vaginalis, and its phylogenetic inferences. Mitogenome composition was 16,352 bp in length and its overall base composition in the circular genome was A = 33.2%, T = 29.0%, C = 24.50% and G = 13.30%. It exhibited a typical mitogenome str...

journal_title：Molecular biology reports

authors： Singh B,Kumar A,Uniyal VP,Gupta SK

更新日期：2019-02-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease, with multiple genetic and environmental factors involving in its etiology. Angiotensin converting enzyme (ACE) gene was reported to have important roles in the development and progression of SLE. In this study, a case-control study was carried out to investi...

journal_title：Molecular biology reports

authors： Gong AM,Li XY,Wang YQ,Yan HX,Xu ZX,Feng Z,Xie YQ,Yin DH,Yang SZ

更新日期：2012-10-01 00:00:00

abstract：:Recently genetics and epigenetics alterations have been found to be characteristic of malignancy and hence can be used as targets for detection of neoplasia. RAS association domain family protein 1A (RASSF1A) gene hypermethylation has been a subject of interest in recent researches on cancer breast patients. The aim o...

journal_title：Molecular biology reports

authors： Hagrass HA,Pasha HF,Shaheen MA,Abdel Bary EH,Kassem R

更新日期：2014-01-01 00:00:00

abstract：:Interleukin 15 (IL-15) is a Th1-related cytokine that triggers inflammatory cell recruitment with implications for pathogenesis in ulcerative colitis. The IL-15 gene is located within a 35 kb region of the q28-31 locus of chromosome 4. In the present work, the role of IL-15 gene polymorphisms as susceptibility markers...

journal_title：Molecular biology reports

authors： Yamamoto-Furusho JK,De-León-Rendón JL,Alvarez-León E,Valdivia-Eziquio M,Obil-Chavarría C,Vargas-Alarcón G

更新日期：2014-01-01 00:00:00

abstract：:Glutamate represents the main excitatory neurotransmitter in the mammalian brain; however, its excessive elevation in the extracellular space is cytotoxic and can result in neuronal death. The ischemia initiated brain damage reflects changes in glutamate concentration in peripheral blood. This paper investigated the r...

journal_title：Molecular biology reports

authors： Jachova J,Gottlieb M,Nemethova M,Bona M,Bonova P

更新日期：2020-07-01 00:00:00

abstract：:1-aminocyclopropane-1-carboxylic acid oxidase (ACO) enzyme is a member of the Fe II-dependent family of oxidases/oxygenases which require Fe(2+) as a cofactor, ascorbate as a cosubstrate and CO(2) as an activator. This enzyme catalyses the terminal step in the plant signaling of ethylene biosynthetic pathway. A 948 bp...

journal_title：Molecular biology reports

authors： Jafari Z,Haddad R,Hosseini R,Garoosi G

更新日期：2013-02-01 00:00:00

abstract：:Two genes HPC/ELAC-2 and AAT were studied in north Indian population. HPC/ELAC-2 was studied in prostate cancer cases and AAT was studied in COPD patients. HPC/ELAC-2 is considered as an important cancer-susceptibility gene in prostate cancer. There are two common polymorphisms of this gene, i.e., Ser217Leu and Ala541...

journal_title：Molecular biology reports

authors： Sobti RC,Thakur H,Gupta L,Janmeja AK,Seth A,Singh SK

更新日期：2011-06-01 00:00:00

abstract：:By measuring the permeability across the lipid bilayer in the presence and absence of membrane-bound protein or glycoprotein it should be possible to get an impression concerning their ability to penetrate into the membrane bilayer. Proteins such as phospholipase A2 and acetylcholinesterase markedly increase the perme...

journal_title：Molecular biology reports

authors： Neitchev VZ,Bideaud FA

更新日期：1982-04-16 00:00:00

abstract：:The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) currently is one of the economically most destructive pest species of stone and pome fruits worldwide. Here we sequenced the complete mitochondrial genome of this pest. This genome is 15,776 bp long, with an A + T content of 81.24%, contain...

journal_title：Molecular biology reports

authors： Gong YJ,Shi BC,Kang ZJ,Zhang F,Wei SJ

更新日期：2012-03-01 00:00:00

abstract：:This study is aimed to identify polymorphic microsatellite markers and establish their potential for population genetics studies in three carp (family cyprinidae; subfamily cyprininae) species, Labeo rohita, Catla catla and Cirrhinus mrigala through use of cyprinid primers. These species have high commercial value and...

journal_title：Molecular biology reports

authors： Masih P,Luhariya RK,Das R,Gupta A,Mohindra V,Singh RK,Srivastava R,Chauhan UK,Jena JK,Lal KK

更新日期：2014-08-01 00:00:00

abstract：:This novel orange fluorescent protein (OFP) emits brilliant orange fluorescent light. OFP has high fluorescence quantum yield, fast maturation rate, and stability, which imply this protein should be the most favorable biotechnological tools used to investigate the function of target gene by visualizing, monitoring, an...

journal_title：Molecular biology reports

authors： Liu JM,David WC,Ip DT,Li XH,Li GL,Wu XF,Yue WF,Zhang CX,Miao YG

更新日期：2009-02-01 00:00:00

abstract：:Latexin, a carboxypeptidase A inhibitor, is expressed in a cell type-specific manner in both central and peripheral nervous systems in the rat. It is used as a molecular marker for the regional specification of the neocortex. In this study, a cDNA was isolated from a human fetal brain cDNA library. The cDNA (LXN) cont...

journal_title：Molecular biology reports

authors： Liu Q,Yu L,Gao J,Fu Q,Zhang J,Zhang P,Chen J,Zhao S

更新日期：2000-01-01 00:00:00

abstract：:Maintenance of glucose homeostasis is reciprocally regulated by insulin and glucagon-like peptide-1 (GLP-1). We previously reported that GLP-1 secretion in response to an oral glucose load was impaired following an administration of 3-deoxyglucosone (3DG), an independent factor associated with the development of pre-d...

journal_title：Molecular biology reports

authors： Song X,Zhou L,Xu H,Wang F,Liang G,Zhang L,Huang F,Jiang G

更新日期：2019-10-01 00:00:00

abstract：:Tall fescue is a perennial cool-season grass with economic importance especially in temperate regions of the northern hemisphere. This study was done to assess the genetic diversity and population structure of 90 tall fescue populations and cultivars using ISSR and EST-SSR markers in order to categorize valuable popul...

journal_title：Molecular biology reports

authors： Shahabzadeh Z,Mohammadi R,Darvishzadeh R,Jaffari M

更新日期：2020-01-01 00:00:00

abstract：:Free cytoplasmic 40S mRNP particles from rat liver were treated with EDTA and separated into two populations of RNP particles with sedimentation maxima of 20S and 35S, respectively. A characteristic set of distinct scRNAs is found for 20S and 35S RNP particles. The sequences of two of the most abundant scRNAs from 20S...

journal_title：Molecular biology reports

authors： Villringer A,Kühn B,Schneider K,Andus T,Michels K,Northemann W,Krupp G,Walckhof B,Gross HJ,Klump H,Kroll A,Branlant C,Heinrich PC

更新日期：1983-05-01 00:00:00

abstract：:Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been ...

journal_title：Molecular biology reports

authors： Ascari IJ,Martins SC,Camargo LSA,Mendez-Otero R,Jasmin

更新日期：2018-12-01 00:00:00