Abstract:
:One of the most crucial steps for preventing viral pandemics is the early detection of the causative virus on site. Various molecular and immunological approaches have been developed for virus detection. In this study, we investigated the utility of the recently introduced convection polymerase chain reaction (cPCR) platform for the rapid and sensitive detection of various animal viruses in the field, including the foot-and-mouth disease virus (FMDV) and avian influenza viruses (AIVs). Primer sets were designed to simultaneously detect two highly conserved regions of the FMDV, including the 5' untranslated region (5'-UTR) and 3D gene, and to specifically amplify the NP and hemagglutinin (HA) genes of H5 and H9 subtypes of AIVs. The portable cPCR system was able to amplify from as low as 1 to 10 copies of viral cDNAs in the singleplex mode and 10 to 100 copies of viral cDNAs in the duplex mode within 21 min. Thus, our data suggest that the cPCR protocols developed in this study are highly sensitive and enable quick detection of animal viruses in biological samples.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Lee MH,Song KY,Hwang HJ,Kim JH,Hwang Idoi
10.1007/s11033-019-04961-xsubject
Has Abstractpub_date
2019-10-01 00:00:00pages
5073-5077issue
5eissn
0301-4851issn
1573-4978pii
10.1007/s11033-019-04961-xjournal_volume
46pub_type
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