Abstract:
:Ribosomes were isolated from baby hamster kidney fibroblasts, either 20 min or 2 days after labelling with radioactive amino acids, and their proteins subjected to two-dimensional polyacrylamide gel electrophoresis. No significant differences were observed between the amounts of radioactivity associated with the position of the phosphorylated derivatives of protein S6. This suggests that the phosphorylation is unlikely to be important in ribosomal biogenesis or extranuclear transport.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Kennedy IM,Leader DPdoi
10.1007/BF00777524subject
Has Abstractpub_date
1980-12-31 00:00:00pages
199-202issue
4eissn
0301-4851issn
1573-4978journal_volume
6pub_type
杂志文章abstract::Consistent with its precloning characterization from the cellulolytic Bacillus sp., beta-1,4-endoglucanase purified from the recombinant E. coli exhibited maximum activity at 60 degrees C and pH 7.0. It was highly specific for CMC hydrolysis, with stability up to 70 degrees C and over a pH range of 6.0-8.0. The K(m) a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9592-5
更新日期:2010-04-01 00:00:00
abstract::Several mechanisms are involved in the loss of cellular integrity and tissue destructions in various brain regions during ischemic insult. The affected brain employs various self-repair mechanisms during the poststroke recovery. Therefore, the current study involves time course changes in different brain regions follo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05060-7
更新日期:2019-12-01 00:00:00
abstract::The antibacterial and anticancerous properties of EMTAHDCA have already been reported in our previous study. However, mode of action of EMTAHDCA is still elusive. The present study was aimed to investigate the molecular targets in Escherichia coli and spleen of lymphoma-bearing mice in response to cyanocompound 9-ethy...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04659-0
更新日期:2019-06-01 00:00:00
abstract::Interferon gamma (IFN-γ) is an important immunoregulatory cytokine that has a central role against viral and bacterial infections. In this study, the cDNA encoding 141 amino acids of mature IFN-γ from mice splenocytes was cloned in a prokaryotic expression vector pQE 30. Optimization of expression conditions resulted ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3537-3
更新日期:2014-10-01 00:00:00
abstract::The programmed cell death (PCD) is important in maintaining the cell number homoeostasis of tissues and organs in metazoan. This process is regulated by the inhibitors of apoptosis proteins (IAPs). The function and mechanism of IAPs have been well studied in animal embryonic development and human cancers. However, the...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0909-9
更新日期:2012-02-01 00:00:00
abstract::Transition from laying to incubation behavior in chicken is an interesting topic in reproductive biology. The decline of incubation behavior in chicken population has led to considerable phenotypic differences in reproductive traits between breeds. However, the exact genetic mechanism of the reproductive phase transit...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-016-4033-8
更新日期:2016-09-01 00:00:00
abstract::Breast cancer is one of the most frequently diagnosed cancers and the leading cause of cancer deaths among females across the world, accounting for 23 % (1.38 million) of total new cancer cases and 14 % (0.45 million) of the total cancer deaths in 2008. c-kit is expressed in mast cell growth factor, cellular migration...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1786-6
更新日期:2012-09-01 00:00:00
abstract::Current study was conducted to investigation of effect of magnetic field on cell dedifferentiation and follow it callus induction derived from mature embryo culture in bread wheat genotypes. For this purpose, a factorial experiment based on completely randomized design was carried out with two wheat genotypes and thre...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2214-7
更新日期:2013-02-01 00:00:00
abstract::The aim of this study was to determine whether tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) promoter polymorphisms confer susceptibility to periodontitis in ethnically different populations. A literature search was performed using PubMed and Embase and a meta-analysis of the identified studies was conducte...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-013-2621-4
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abstract::The cell divisions cycle 42 (Cdc42) gene has been characterized in the fungi, such as Candida albicans, Penicillium marneffei, and Wangiella (Exophiala) dermatitidis, which plays important roles during growth and development. The partial cDNA sequence of Cdc42 of Fonsecaea monophora (F. monophora), designated FmCdc42,...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0806-2
更新日期:2012-02-01 00:00:00
abstract::Finger millet (Eleusine coracana L.) is an important crop used for food, forage, and industrial products. Three DNA marker techniques, random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and cytochrome P(450) gene based markers were used for the detection of genetic polymorphism in 83 accessions of f...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0067-5
更新日期:2010-12-01 00:00:00
abstract::Circadian rhythms are endogenous and self-sustained oscillations of multiple biological processes with approximately 24-h rhythmicity. Circadian genes and their protein products constitute the molecular components of the circadian oscillator that form positive/negative feedback loops and generate circadian rhythms. Th...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2841-7
更新日期:2014-01-01 00:00:00
abstract::Knowledge in the area of genetic diversity could aid in providing useful information in the selection of material for breeding such as hybridization programs and quantitative trait loci mapping. To this end, 50 Nicotiana tabacum genotypes were genotyped with 21 primer combination of amplified fragment length polymorph...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3194-6
更新日期:2014-05-01 00:00:00
abstract::This study is to explore the relationship between vascular endothelial growth factor (VEGF) and pathological changes in cryptorchidism by using murine model of intraperitoneal cryptorchidism with surgical operation. To investigate the relationship between the changes of VEGF expression and heat stress inducing germ ce...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05454-y
更新日期:2020-05-01 00:00:00
abstract::It is well known that transcriptionally inactive rRNA genes are correlated with DNA hyper-methylation and histone hypo-methylation and there is clear evidence in humans that DNA and histone modification which alter chromatin structure are related to chromosome fragility. Very little is known about the biological cause...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2112-z
更新日期:2013-01-01 00:00:00
abstract::Combined treatment with niacin and chromium has been found to have a protective effect against oxidative damage to different tissues of hyperlipidemic rats. But its effects on vascular endothelial dysfunction are less clear. This study was performed to investigate the effect of combined treatment with niacin and chrom...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9309-1
更新日期:2009-07-01 00:00:00
abstract::Rice bran oil is good quality edible oil, rich in antioxidants and comprised typically of oleic-linoleic type fatty acids. However, presence of a highly lipolytic enzyme Phospholipase D alpha1 (OsPLDα1) increases free fatty acid content in the oil which further leads to stale flavor and rancidity of the oil, making it...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05144-4
更新日期:2020-01-01 00:00:00
abstract::Metallothioneins are rather ubiquitous metal-binding proteins induced by stressing or physiological stimuli. Two major metallothionein isoforms have been identified in mussel: MT10 and MT20. Nevertheless the high sequence homology, the two isoforms exhibit different expression and inducibility in vivo. We cloned and p...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-006-9009-7
更新日期:2006-12-01 00:00:00
abstract::The quantitative changes of double-stranded RNA components of nuclear ribonucleo-protein particles containing pre-mRNA was investigated in the course of incubation of particles at 37 degrees C. The incubation of purified nuclear particles revealed the fragmentation of long double-stranded RNA sequences into shorter st...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00423235
更新日期:1976-11-01 00:00:00
abstract::Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0736-z
更新日期:2012-01-01 00:00:00
abstract::1-aminocyclopropane-1-carboxylic acid oxidase (ACO) enzyme is a member of the Fe II-dependent family of oxidases/oxygenases which require Fe(2+) as a cofactor, ascorbate as a cosubstrate and CO(2) as an activator. This enzyme catalyses the terminal step in the plant signaling of ethylene biosynthetic pathway. A 948 bp...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2178-7
更新日期:2013-02-01 00:00:00
abstract::The stem cells in freshwater flatworms (planarian) are called neoblasts. Neoblasts are capable of proliferation and differentiation into every cell type, including the gametes. For the investigation of the mechanisms of stem cells proliferation and differentiation the proper evaluation of changes in the cell cycle of ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1070-1
更新日期:2012-03-01 00:00:00
abstract::A cDNA encoding an O-methyltransferase (namely FGCOMT1) was identified from the medicinal plant Trigonella foenum-graecum L. The FGCOMT1 enzyme is a functional caffeic acid O-methyltransferase (COMT) and is localized in the cytosol. Kinetic analysis indicated that FGCOMT1 protein exhibited the highest catalyzing effic...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0899-7
更新日期:2012-02-01 00:00:00
abstract::Fusarium head blight (FHB), predominantly caused by Fusarium graminearum, is a destructive disease that poses a serious threat to wheat (Triticum aestivum L.) production around the world. A suppression subtractive hybridization (SSH) cDNA library was constructed from F. graminearum infected spikes of a resistant Belgi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1823-5
更新日期:2012-10-01 00:00:00
abstract::The binding characteristics of the interaction between 3-(2-cyanoethyl) cytosine (CECT) and human serum albumin (HSA) were investigated using fluorescence, UV absorption spectroscopic and molecular modeling techniques under simulative physiological conditions. The intrinsic fluorescence intensity of HSA was decreased ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1855-x
更新日期:2012-11-01 00:00:00
abstract::Two calcium carbonate crystal polymorphs, aragonite and calcite, are the main inorganic components of mollusk shells. Some fossil evidences suggest that aragonite shell is more ancient than calcite shell for the Bivalvia. But, the molecular biology evidence for the above deduction is absent. In this study, we searched...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3620-9
更新日期:2014-11-01 00:00:00
abstract::In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis-inducing protein, as a GST-fusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death. The HAP protein inhibited the bacterial growth within 30 min after inducting HAP expression. The transmission e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/b:mole.0000043551.84883.c8
更新日期:2004-09-01 00:00:00
abstract::The influence of peptidyl-tRNA on the dissociation of yeast 80 S ribosomes into subunits was studied. For this purpose temperature-sensitive (ts) suppressor strain of yeast Saccharomyces cervisiae carrying a defect in peptide chain termination was used. It was found that peptidyl-tRNA did not influence the dissociatio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775964
更新日期:1978-06-16 00:00:00
abstract::An ORF having a potential to code for a polypeptide of 79 amino acids has been identified within 993 nt sequence of 2 kb EcoRI-W fragment of Spodoptera litura nucleopolyhedrovirus (SpltNPV-I). Nucleotide and deduced amino acid sequence analyses showed its identity with the ubiquitin homologue of eukaryotes (79-80%), M...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-005-0887-x
更新日期:2004-12-01 00:00:00
abstract::Various efforts made to stop the deadly epidemic of HIV since its discovery in 1983 remain unsuccessful and this virus still continues to claim the lives of millions of individuals every year. The viral effect in the cell is complicated and the overall disease outcome is the result of interaction between a few viral p...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9698-9
更新日期:2010-01-01 00:00:00