Abstract:
:Interferon gamma (IFN-γ) is an important immunoregulatory cytokine that has a central role against viral and bacterial infections. In this study, the cDNA encoding 141 amino acids of mature IFN-γ from mice splenocytes was cloned in a prokaryotic expression vector pQE 30. Optimization of expression conditions resulted in high IFN-γ protein. Western blot showed that recombinant IFN-γ was specifically recognized by its counterpart anti-mouse IFN-γ antibodies. In vitro dose-dependent studies, with A549 and HeLa cell lines, showed that cloned IFN-γ was safe and had no effect on cell proliferation. The protein prediction and analysis using SOPMA program, revealed that IFN-γ had 80 α-helices, 8 β-turns jointed by 9 extended strands and 44 random coils. A total of four major clusters were observed with murine IFN-γ sharing 39 % homology with human IFN-γ. Pair-wise alignment studies with human revealed 26 % identity and 43.3 % similarity. The recovery of bioactive proteins from inclusion bodies (IBs) is a complex process and various protocols have been developed. We report here a simple, robust and inexpensive purification approach for obtaining recombinant IFN-γ protein expressed as IBs in E.coli.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Kumar M,Singh M,Singh SBdoi
10.1007/s11033-014-3537-3subject
Has Abstractpub_date
2014-10-01 00:00:00pages
6537-43issue
10eissn
0301-4851issn
1573-4978journal_volume
41pub_type
杂志文章abstract::Published data on the association of vascular endothelial growth factor (VEGF) -1154G>A polymorphism with cancer risk is inconclusive. To derive a more precise estimation of association between VEGF -1154G>A polymorphism and the risk of cancer, we performed a meta-analysis of 7,071 cancer cases and 7,693 controls from...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-1326-9
更新日期:2012-05-01 00:00:00
abstract::Accurate normalization of gene expression with qRT-PCR depends on the use of appropriate reference genes (RGs) for the species under a given set of experimental conditions. Multiple RGs for gene expression analysis of soybean exposed to heavy metal stress treatment have not been reported in the literature. In this stu...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0897-9
更新日期:2012-02-01 00:00:00
abstract::Two new thermophilic type II restriction endonucleases, which we designated as Bsu2413I and Bfi2411I, have been isolated from gram-positive thermophilic bacteria Bacillus subtilis strain 2413 and Bacillus firmus strain 2411 respectively and partially purified. The restriction endonucleases were extracted from cell ext...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/b:mole.0000031503.19851.3b
更新日期:2004-06-01 00:00:00
abstract::Body measurement and meat quality traits which play important roles in the assessment of productivity and economy in cattle were influenced by genes and environmental factors. Latest studies showed that LYR motif containing 1 (LYRM1) may be involved in influencing fatness deposition in animals. The objective of this s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2195-6
更新日期:2013-02-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is an autoimmune disease, with multiple genetic and environmental factors involving in its etiology. Angiotensin converting enzyme (ACE) gene was reported to have important roles in the development and progression of SLE. In this study, a case-control study was carried out to investi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1813-7
更新日期:2012-10-01 00:00:00
abstract::Probiotic bacteria must overcome the toxicity of bile salts secreted in the gut and adhere to the epithelial cells to enable their better colonization with extended transit time. Expression of bile salt hydrolase and other proteins on the surface of probiotic bacteria can help in better survivability and optimal funct...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1006-9
更新日期:2012-03-01 00:00:00
abstract::This meta-analysis was performed to assess the relationships between the PON1 Q192R (rs662 T>C) polymorphism and the clinical outcome of antiplatelet treatment after percutaneous coronary intervention (PCI). A range of electronic databases were searched: Web of Science (1945-2013), the Cochrane Library Database (Issue...
journal_title:Molecular biology reports
pub_type: 撤回出版物,收录出版
doi:10.1007/s11033-014-3509-7
更新日期:2014-09-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is a complex human disease influenced by multiple genes and environmental factors. The SERPINE2 gene has recently been demonstrated to be associated with COPD onset in a non-East Asian population. In this study, we genotyped 20 single nucleotide polymorphisms (SNPs) in SERP...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0877-0
更新日期:2012-02-01 00:00:00
abstract::Recent studies suggest that the common variant in the GSTM1 and GSTT1 genes modifies the risk of coronary artery disease (CAD), however, it is unclear whether the risk of CAD modulated by variants in the GSTM1 and GSTT1 genes was associated with alterations of indices of oxidative stress and inflammation. Our study is...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9877-8
更新日期:2010-01-01 00:00:00
abstract::Endometriosis is a polygenic and multifactorial gynecology situation which might be associated with angiogenesis. In the current study we assess the role of vascular endothelial growth factor (VEGF) - 2578 A/C, and + 936 C/T polymorphisms in susceptibility to endometriosis and checking the expression of VEGF mRNA in e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04807-6
更新日期:2019-06-01 00:00:00
abstract::Membrane-bound ribosomes were isolated from a post-mitochondrial supernatant fraction of mouse liver homogenate by sedimentation in a sucrose density gradient, Loose ribosomes were released from the membrane fragments with 0.5 M KCl, while tight bound ribosomes were not released. After purification of the loose and ti...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00778433
更新日期:1980-07-31 00:00:00
abstract::Lung cancer including non-small cell lung cancer (NSCLC) and small cell lung cancer is one of the most aggressive tumors with high incidence and low survival rate. The typical NSCLC patients account for 80-85 % of the total lung cancer patients. To systemically explore the molecular mechanisms of NSCLC, we performed a...
journal_title:Molecular biology reports
pub_type: 杂志文章,收录出版
doi:10.1007/s11033-014-3705-5
更新日期:2014-12-01 00:00:00
abstract::Dyggve melchior clausen syndrome (DMC, MIM 223800) is a very rare autosomal recessive form of skeletal dysplasia associated with various degrees of mental retardation. It is characterized by a progressive spondyloepimetaphyseal dysplasia (SEMD) with disproportionate short stature, generalized platyspondyly and lacy il...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05774-z
更新日期:2020-09-01 00:00:00
abstract::Dihydroflavonol 4-reductase (DFR), which catalyzes the reduction of dihydroflavonols to leucoanthocyanins, is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids of importance in plant development and human nutrition. This study isolated a full length cDNA encoding DFR, designat...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1061-2
更新日期:2012-03-01 00:00:00
abstract::The lily-of-the-valley Convallaria (Asparagaceae) consists of three herbaceous perennial species. The plants are commonly found in northern hemisphere, and are best-known for their ornamental and pharmaceutical value. In order to assess the genetic structure, diversity and demographic history of Convallaria species, 1...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05376-9
更新日期:2020-04-01 00:00:00
abstract::Growing data supported that epigenetic modifications, including altered DNA methylation have a potential role in the pathogenesis of autoimmune thyroid diseases (AITD). In the present study we aimed to investigate the methylation status of ICAM-1 gene promoter in patients with AITD. Forty patients with Graves' disease...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04990-6
更新日期:2019-10-01 00:00:00
abstract::Telomeres are protective cap on the ends of DNA of non-coding tandem repeats of TTAGGG. Human telomerase reverse transcriptase (hTERT) is a catalytic subunit of telomerase that maintains the structure of telomeres. Type 2 diabetes (T2D) affects multi-organ and telomere length by altering telomerase activity. We aimed ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-06045-7
更新日期:2021-01-03 00:00:00
abstract::CENP-A locates at nucleosome as histone H3-like proteins, and is phosphorylated during mitosis. We investigated the dynamic distribution of p-CENP-A to explore the details of its function. We found that p-CENP-A was phosphorylated at late prophase, and the signal of p-CENP-A arranged at equatorial plate along with nuc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9136-9
更新日期:2008-12-01 00:00:00
abstract::The plasticity of the central nervous system helps form the basis for the neurobiology of learning and memory. Long-term potentiation (LTP) is the main form of synaptic plasticity, reflecting the activity level of the synaptic information storage process, and provides a good model to study the underlying mechanisms of...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-010-0128-9
更新日期:2011-01-01 00:00:00
abstract::F-box proteins are key components of the ubiquitin (Ub)/26S proteasome pathway that mediates selective degradation of regulatory proteins involved in a wide variety of cellular processes affecting eukaryotic cells. In plants, F-box genes form one of the largest multigene superfamilies and control many important biolog...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0438-y
更新日期:2011-06-01 00:00:00
abstract::Stats are latent transcription factors involved in normal cellular signaling in response to cytokine or growth factor stimulation. Constitutive activation of Stats (primarily Stat3 and Stat5) has been implicated in growth dysregulation and oncogenesis. Furthermore, increased activation of Stats has been observed in se...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/b:mole.0000013503.16301.82
更新日期:2004-03-01 00:00:00
abstract::FK-506 binding proteins (FKBPs) belong to the peptidyl-prolyl cis/trans isomerase superfamily (PPIases, EC: 5.2.1.8) which catalyzes the interconversion of peptidyl-prolyl bonds while they can also act on polypeptides, as folding helper enzymes. Here, we biochemically characterize two recombinant FKBPs, AvfkbA1 and Av...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1869-4
更新日期:2012-12-01 00:00:00
abstract::Narrow-leafed lupin (Lupinus angustifolius L.) is used as grain legumes, fodder for livestock and green manure in the world and has a great potential to be developed as a new crop in China. In this study, we assessed the genetic diversity among a set of 109 newly introduced accessions of narrow-leafed lupin using 76 g...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05596-z
更新日期:2020-07-01 00:00:00
abstract::45S ribosomal precursor RNA and large heterogeneous RNA molecules (greater than 45S) extracted from human leukemic cells were incubated in vitro with purified RNase III, which specifically attacks double-helical RNA regions. About 50% of the ribosomal precursor was cleaved into two major fragments sedimenting at 28S a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00808381
更新日期:1977-12-01 00:00:00
abstract::CYP1B1 encodes an estrogen enzyme that oxidizes 17β-estradiol to 4-hydroxyestradiol. The evidence demonstrates there may be a relationship between CYP1B1 and thyroid function. To date, no study has evaluated if genetic polymorphisms that regulate concentrations of serum FT3 and FT4 contribute to Polycyctic Ovary Syndr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2406-1
更新日期:2013-04-01 00:00:00
abstract::Complexity is one of the hallmarks that applies to C2H2 type zinc finger proteins (ZFPs). Structurally distinct clusters of zinc finger modules define an extremely large superfamily of nucleic acid binding proteins with several hundred, perhaps thousands of different members in vertebrates. Recent discoveries have pro...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/BF00999848
更新日期:1994-07-01 00:00:00
abstract::Serotonin receptor (5-HT) is a biogenic amine acting as a neurotransmitter and neuromodulator that mediates various aspects of reproduction and gametogenesis. The full-length nucleotide sequence of Haliotis discus hannai encodes a protein of 417 amino acids with a predicted molecular mass of 46.54 kDa and isoelectric ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05162-2
更新日期:2020-01-01 00:00:00
abstract::Fluorescent quantum dots (QDs) have been widely applied in biological and biomedical areas, but relatively little is known about the interaction of QDs with some natural enzymes. Herein, the interactions between 3-mercaptopropionic acid-capped CdTe QDs (MPA-QDs) and papain were systematically investigated by UV-Vis ab...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2681-5
更新日期:2013-10-01 00:00:00
abstract::Plumbagin (PLB) is a phytochemical being used for centuries in traditional medicines. Recently, its capacity to inhibit the development of human tumors has been observed, through the induction of apoptosis, cell cycle arrest, and inhibition of angiogenesis and metastasis. Here we evaluated the mechanism of action of P...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05042-9
更新日期:2019-12-01 00:00:00
abstract::Staudtia kamerunensis (Myristicaceae) or 'Niové' is an evergreen tree widespread in Central African moist forests. The bark and seeds are used in traditional medicine, yet the tree is mainly harvested for its high quality, multi-purpose timber. To facilitate sustainable harvesting and conservation of the species, we a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4239-z
更新日期:2018-10-01 00:00:00