Abstract:
:Accurate normalization of gene expression with qRT-PCR depends on the use of appropriate reference genes (RGs) for the species under a given set of experimental conditions. Multiple RGs for gene expression analysis of soybean exposed to heavy metal stress treatment have not been reported in the literature. In this study, we evaluated the expression stability of ten candidate RGs in leaves, roots and stems of two soybean cultivars exposed to cadmium (Cd). Based on the geNorm and NormFinder analysis, ACT3, PP2A, ELF1B and F-box were the most stable RGs in these gene expression studies. In contrast, G6PD, UBC2, TUB, and ELF1A were the most variable ones and should not be used as RGs in these experimental conditions.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Wang Y,Yu K,Poysa V,Shi C,Zhou Ydoi
10.1007/s11033-011-0897-9subject
Has Abstractpub_date
2012-02-01 00:00:00pages
1585-94issue
2eissn
0301-4851issn
1573-4978journal_volume
39pub_type
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