Ribosome-membrane interactions: characterization of ribosomal proteins from loose and tight bound ribosomes.

abstract：:Experiments with 5'-azacytidine and hematopoietic growth factor approved for the transformation of human mesenchymal cells into hematopoietic cells have demonstrated that cell fate can be dramatically altered by changing the epigenetic state of cells. Here, we demonstrate that umbilical cord-derived human mesenchymal ...

journal_title：Molecular biology reports

authors： Hu K,Xu C,Ni H,Xu Z,Wang Y,Xu S,Ji K,Xiong J,Liu H

更新日期：2014-07-01 00:00:00

abstract：:Several lines of evidence indicate that inflammatory processes play pivotal role in the development of intracranial aneurysm (IA). Recently, polymorphisms in the interleukin-12 (IL-12) gene were shown to be associated with immune-mediated inflammatory disease. The aim of this study was to investigate the interactions ...

journal_title：Molecular biology reports

authors： Li LJ,Pan XM,Sima X,Li ZH,Zhang LS,Sun H,Zhu Y,Liang WB,Gao LB,Zhang L

更新日期：2012-12-01 00:00:00

abstract：:Chronic obstructive pulmonary disease (COPD) is a common, complex disorder associated with substantial morbidity and mortality, influenced by both environmental factor and genetic factor. ADAM33 gene was found to be associated with asthma, declined lung function and COPD. The purpose of the study was to test whether S...

journal_title：Molecular biology reports

authors： Xiao J,Han J,Wang X,Hua D,Su D,Bao Y,Lv F

更新日期：2011-11-01 00:00:00

abstract：:Two GATC specific methylases together with Sau3AI isoschizomeric restriction endonuclease were partially characterized in Mitsuokella multiacida 46/5. This is the first report on the presence of solitary Dam methyltransferase alongside GATC specific restriction-modification system resulting in the unusual two-fold met...

journal_title：Molecular biology reports

authors： Piknova M,Filova M,Javorsky P,Pristas P

更新日期：2005-12-01 00:00:00

abstract：:A full length spermidine synthase (PgSPD) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The cDNA was 1,188 nucleotides long and had an open reading frame of 1,002 bp with a deduced amino acid sequence of 333 residues. The calculated molecular mass of the matured protein is approximate...

journal_title：Molecular biology reports

authors： Parvin S,Kim YJ,Pulla RK,Sathiyamoorthy S,Miah MG,Kim YJ,Wasnik NG,Yang DC

更新日期：2010-02-01 00:00:00

abstract：:Studies investigating the association between glutathione S-transferase M1 (GSTM1) polymorphism and bladder cancer risk report conflicting results. The objective of this study was to quantitatively summarize the evidence for such a relationship. We performed a systematic search of the National Library of Medline and E...

journal_title：Molecular biology reports

authors： Zhang R,Xu G,Chen W,Zhang W

更新日期：2011-04-01 00:00:00

abstract：:Two DREB-like genes, designated as DmDREBa and DmDREBb, were isolated from chrysanthemum by RACE approach. The DmDREBa and DmDREBb genes encoded proteins of 191 and 185 amino acid residues with the predicted molecular mass of 21.66 and 20.99 kDa, respectively. Both DmDREBs proteins comprised a typical EREBP/AP2 domain...

journal_title：Molecular biology reports

authors： Yang Y,Wu J,Zhu K,Liu L,Chen F,Yu D

更新日期：2009-01-01 00:00:00

abstract：:Ribonuclease P (RNase P) is a key enzyme involved in tRNA biosynthesis. It catalyses the endonucleolytic cleavage of nearly all tRNA precursors to produce 5'-end matured tRNA. RNase P activity has been found in all organisms examined, from bacteria to mammals. Eubacterial RNase RNA is the only known RNA enzyme which f...

journal_title：Molecular biology reports

authors： Drainas D

更新日期：1995-01-01 00:00:00

abstract：:To determine whether methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms are associated with pre-eclampsia susceptibility. Literature searches of the Pubmed, Embase, BIOSIS Previews and Web of Science were conducted to identify all eligible articles up to January 18th, 2013. The pooled odds rati...

journal_title：Molecular biology reports

authors： Li X,Luo YL,Zhang QH,Mao C,Wang XW,Liu S,Chen Q

更新日期：2014-08-01 00:00:00

abstract：:Three members of Brassica napus TRANSPARENT TESTA 2 (BnTT2) gene family encoding potential R2R3-MYB regulatory proteins of proanthocyanidin biosynthesis were isolated. BnTT2-1, BnTT2-2, and BnTT2-3 are 1102 bp with two introns, and have a 938-bp full-length cDNA with a 260 amino acid open reading frame. They share 98....

journal_title：Molecular biology reports

authors： Wei YL,Li JN,Lu J,Tang ZL,Pu DC,Chai YR

更新日期：2007-06-01 00:00:00

abstract：:TopBP1 protein displays structural as well as functional similarities to BRCA1 and is involved in DNA replication, DNA damage checkpoint response and transcriptional regulation. Aberrant expression of TopBP1 may lead to genomic instability and can have pathological consequences. In this study we aimed to investigate e...

journal_title：Molecular biology reports

authors： Forma E,Krzeslak A,Bernaciak M,Romanowicz-Makowska H,Brys M

更新日期：2012-07-01 00:00:00

abstract：:Long non-coding RNAs (LncRNAs) are non-coding RNAs. The potential roles of lncRNAs in type 2 diabetes mellitus (T2DM) are not well-known. In this study, we aim to assess the expression levels of LY86-AS1 and HCG27_201 in T2DM patients and a healthy control group. We obtained whole blood and serum samples from 100 T2DM...

journal_title：Molecular biology reports

authors： Saeidi L,Ghaedi H,Sadatamini M,Vahabpour R,Rahimipour A,Shanaki M,Mansoori Z,Kazerouni F

更新日期：2018-12-01 00:00:00

abstract：:The RNA-binding protein Arabidopsis thaliana glycine-rich RNA-binding protein 7 (AtGRP7) regulates the steady-state abundance of numerous target transcripts in A. thaliana. Here we show that the GA1 and GA2 transcripts encoding the first enzymes of the gibberellin biosynthetic pathway are expressed at reduced levels i...

journal_title：Molecular biology reports

authors： Löhr B,Streitner C,Steffen A,Lange T,Staiger D

更新日期：2014-01-01 00:00:00

abstract：:The present study demonstrates that protein biosynthesis can be studied in single isolated human scalp hair follicles. The matrix and the sheath are the main regions where amino acids are built in. Incorporation is linear for at least five hours. The newly synthesized proteins can be separated into a water-soluble, a ...

journal_title：Molecular biology reports

authors： Vermorken AJ,Weterings PJ,Bloemendal H

更新日期：1979-02-15 00:00:00

abstract：:Regulation of the sumoylation system at the level of gene expression has not yet been explored. To begin to define transcriptional regulatory features, the promoter region for the SUMO1 gene was cloned from human genomic DNA and characterized. Initially, a 532 base pair fragment upstream of and including the predicted...

journal_title：Molecular biology reports

authors： Nanos-Webb A,Deyrieux A,Bian XL,Rosas-Acosta G,Wilson VG

更新日期：2010-03-01 00:00:00

abstract：:Transcriptome from high throughput sequencing-by-synthesis is a good resource of molecular markers. In this study, we present utility of massively parallel sequencing by synthesis for profiling the transcriptome of red pepper (Capsicum annuum L. TF68) using 454 GS-FLX pyrosequencing. Through the generation of approxim...

journal_title：Molecular biology reports

authors： Lu FH,Cho MC,Park YJ

更新日期：2012-03-01 00:00:00

abstract：:Published data on the association of vascular endothelial growth factor (VEGF) -1154G>A polymorphism with cancer risk is inconclusive. To derive a more precise estimation of association between VEGF -1154G>A polymorphism and the risk of cancer, we performed a meta-analysis of 7,071 cancer cases and 7,693 controls from...

journal_title：Molecular biology reports

authors： Hong TT,Zhang RX,Wu XH,Hua D

更新日期：2012-05-01 00:00:00

abstract：:Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low ex...

journal_title：Molecular biology reports

authors： Schmitt J,Hess H,Stunnenberg HG

更新日期：1993-10-01 00:00:00

abstract：:Ebselen, an organoselenium compound, mimics glutathione peroxidase activity. It is a multifunctional compound, which catalyzes several essential reactions for the protection of cellular components from oxidative and free radical damage. Based on a number of in vitro and in vivo studies, various mechanisms are proposed...

journal_title：Molecular biology reports

authors： Azad GK,Tomar RS

更新日期：2014-08-01 00:00:00

abstract：:We utilized Percoll density gradient centrifugation to isolate and fractionate chloroplasts of Korean winter wheat cultivar cv. Kumgang (Triticum aestivum L.). The resulting protein fractions were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE) coupled with LTQ-FTICR mass spectrometry. This e...

journal_title：Molecular biology reports

authors： Kamal AH,Cho K,Komatsu S,Uozumi N,Choi JS,Woo SH

更新日期：2012-05-01 00:00:00

abstract：:The present study was designed to investigate the protective effect of ferulic acid (FA) against isoproterenol (ISO)-induced cardiac toxicity in rats. Isoproterenol challenged in a dose of 85 mg/kg body weight (b.w.) subcutaneously for two consecutive days in the experimental group resulted in acute cardiac toxicity a...

journal_title：Molecular biology reports

authors： Jain PG,Mahajan UB,Shinde SD,Surana SJ

更新日期：2018-10-01 00:00:00

abstract：:The water-soluble group B3 vitamin nicotinamide (NAM) is involved in a wide range of physical processes through biosynthetically converted to nicotinamide adenine dinucleotide (NAD(+)). In addition to its pivotal role in energy metabolism, NAD(+) is also the indispensable substrate of poly (ADP-ribose) polymerase-1 (P...

journal_title：Molecular biology reports

authors： Zhang XM,Jing YP,Jia MY,Zhang L

更新日期：2012-12-01 00:00:00

abstract：:The product of the OTUB1 gene is a member of the OTU superfamily of predicted cysteine proteases and inhibits cytokine gene transcription via its interaction with a ubiquitin protease and E3 ubiquitin ligase. To further understand the functions of the porcine OTUB1 gene, the subcellular localization of porcine OTUB1 p...

journal_title：Molecular biology reports

authors： Shan TL,Tang ZL,Guo DZ,Yang SL,Mu YL,Ma YH,Guan WJ,Li K

更新日期：2009-07-01 00:00:00

abstract：:Isolated, deproteinized nucleic acids from calf thymus and rat liver nuclei were fractionated on Bio-Gel A-5m into DNA and RNA fractions. Extraction of deproteinized DNA and nuclear RNA with 80% ethanol at pH 9.5 yielded low molecular weight peptides (deprimerones) capable of inhibiting DNA transcription in a cell-fre...

journal_title：Molecular biology reports

authors： Hillar M,Stolzmann Z,Wagle J,Cicconi F,Marmocchi G,Wyborny LE

更新日期：1982-04-16 00:00:00

abstract：:A copper(II) complex containing aspartame (APM) as ligand, Cu(APM)2Cl2·2H2O, was synthesized and characterized. In vitro binding interaction of this complex with human serum albumin (HSA) was studied at physiological pH. Binding studies of this complex with HSA are useful for understanding the Cu(APM)2Cl2·2H2O-HSA int...

journal_title：Molecular biology reports

authors： Shahabadi N,Khodaei MM,Kashanian S,Kheirdoosh F,Filli SM

更新日期：2014-05-01 00:00:00

abstract：:In the post-genomic era, providing a detailed description of protein functions poses a formidable challenge. To gain functional insights, we have to construct many kinds of expression vectors. DNA recombination based on polymerase chain reaction (PCR) and digestion followed by ligation is the preferred method for vect...

journal_title：Molecular biology reports

authors： Pan JS,Zhang ZP,Cai JY,Dong J,Ren JL,Wang XZ

更新日期：2010-03-01 00:00:00

abstract：:Haemoglobin meseenger RNA was isolated from spleen erythroïd cells of anaemic rabbits. The mRNA fraction looks quite homogeneous when analyzed by sucrose gradient centrifugation; it sediments in the 9S region. When added to an ascites cell free system, spleen 9S RNA stimulates the incorporation of radioactive leucine ...

journal_title：Molecular biology reports

authors： Nokin P,Gautier E

更新日期：1973-06-01 00:00:00

abstract：:Many case-control studies have investigated the role of TGF-β1 gene +869C/T promoter polymorphism in autoimmune diseases, but the results are inconsistent. To clarify this point, we performed a meta-analysis based on all available studies in Pubmed, Elsevier Science Direct, Google Searching, Chinese Biomedical Literat...

journal_title：Molecular biology reports

authors： Zhang L,Yan JW,Wang YX,Wan YN,Li JP,Liu P,Xu B,Wang BX,Peng WJ,Pan FM,Wang J

更新日期：2013-08-01 00:00:00

abstract：:In the present study, we characterized an evolutionarily conserved non-transmembrane ATP-binding cassette protein: hABCF3. Subcellular immunofluorescence staining demonstrated that hABCF3 localizes preferentially in cytoplasm, unlike its paralog protein hABCF1, which localizes in both cytoplasm and nucleus. Quantitati...

journal_title：Molecular biology reports

authors： Zhou J,Lin Y,Shi H,Huo K,Li Y

更新日期：2013-10-01 00:00:00

abstract：:It is necessary that genetic markers or biomarkers can be used to predict resistance towards a wide range of infectious diseases. In the present study, we estimated the potential markers and measured their relationship with heritabilities of a wide range of immune traits. Polymorphisms in exon 13 of Mx1, intron 25 of ...

journal_title：Molecular biology reports

authors： Wang SJ,Liu WJ,Sargent CA,Zhao SH,Liu HB,Liu XD,Wang C,Hua GH,Yang LG,Affara NA,Zhang SJ

更新日期：2012-03-01 00:00:00