Abstract：
:Regulation of the sumoylation system at the level of gene expression has not yet been explored. To begin to define transcriptional regulatory features, the promoter region for the SUMO1 gene was cloned from human genomic DNA and characterized. Initially, a 532 base pair fragment upstream of and including the predicted SUMO1 transcription start site (TSS) was cloned and shown to possess promoter activity. Subsequent deletion analysis showed that a smaller fragment containing 158 bp upstream of the TSS region exhibited basal promoter activity in both human and rodent cell lines. Within this basal promoter fragment, there were predicted binding sites for numerous transcription factors, including the nude mouse gene product, Whn (FoxN1). Electrophoretic mobility shift assays showed that Whn could bind to an ACGC motif adjacent to the TSR, and in transfection studies Whn stimulated a 3-fold increase in transcription from this cloned promoter in keratinocytes (HaCaT cells). Mutation of the ACGC motif abrogated both Whn binding and transcriptional activation, indicating that the Whn effect is likely due to direct interaction with this promoter element. Consistent with these observations on the cloned promoter region, Whn also modestly stimulated transcription from the endogenous, genomic SUMO1 promoter in HaCaT cells, consistent with Whn potentially playing a regulatory role for SUMO1 transcription in keratinocytes.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Nanos-Webb A,Deyrieux A,Bian XL,Rosas-Acosta G,Wilson VGdoi
10.1007/s11033-009-9476-8subject
Has Abstractpub_date
2010-03-01 00:00:00pages
1155-63issue
3eissn
0301-4851issn
1573-4978journal_volume
37pub_type
杂志文章abstract：:Wnts are secreted lipid-modified signaling proteins. Activation of Wnt signalling in many tissues has also been associated with cancer. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this st...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-007-9055-9
更新日期：2008-06-01 00:00:00
abstract：:Hypertension is a risk factor for the cardiovascular diseases. Although, several drugs are used to treat hypertension, the success of the antihypertensive therapy is limited. Resveratrol decreases blood pressure in animal models of hypertension. This study researched the mechanisms behind the effects of resveratrol on...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3737-x
更新日期：2015-01-01 00:00:00
abstract：:The majority of the genomic DNA sequence in mammalian and other higher organisms can be transcribed into abundant functional RNA transcripts, especially regulatory non-coding RNAs (ncRNAs) that are expressed in a developmentally and species-specific regulated manner. Here, we review various regulatory non-coding RNAs,...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-014-3259-6
更新日期：2014-06-01 00:00:00
abstract：:The extremely low-frequency magnetic fields (ELF-EMF) are generated by electrical devices and power systems (1 to 300 Hz). In recent decades, exposure to ELF-EMF has emerged potential concerns on public health. Here, we discuss recent progress in the understanding of ELF-EMF biology with a focus on mechanisms of ELF-E...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-020-05563-8
更新日期：2020-07-01 00:00:00
abstract：:A meta-analysis of randomized trials was performed to compare the efficacy of toremifene (TOR) with tamoxifen (TAM) in patients with breast cancer. A total of 4,768 intention-to-treat patients from nine randomized trials were identified, with 2,587 patients in TOR group and 2,181 patients in TAM group. The primary out...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-013-2914-7
更新日期：2014-02-01 00:00:00
abstract：:The aim of this study was to determine whether tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) promoter polymorphisms confer susceptibility to periodontitis in ethnically different populations. A literature search was performed using PubMed and Embase and a meta-analysis of the identified studies was conducte...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-013-2621-4
更新日期：2013-08-01 00:00:00
abstract：:The complete mitochondrial genome 15,650 bp in size of the Deracantha onos has been determined. The gene content, base composition and codon usage of D. onos are coincident to typical hexapods mitochondrial genomes. Genes arrangement of D. onos is identical to Gryllotalpa orientalis, Ruspolia dubia and Anabrus simplex...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-007-9145-8
更新日期：2009-01-01 00:00:00
abstract：:Deprivation of cytokines induces cell cycle arrest and apoptosis in cytokine-dependent hematopoietic progenitors. Previous studies have indicated that in Baf-3, interleukin (IL)-3-dependent cells, apoptosis is caused predominantly by Bim, a BH3-only cell death activator that belongs to the Bcl-2 superfamily. Because B...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-004-7656-0
更新日期：2005-06-01 00:00:00
abstract：:Obesity is for many scholars the most important starting status that gives rise to Metabolic Syndrome (MS) and Type 2 Diabetes (T2D). In the present paper, a genetically homogeneous Amerindian population, as defined by HLA genes, has been genotyped for one of the MS and T2D predisposing genes: PPAR-γ Ala12 and Pro 12 ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2230-7
更新日期：2013-02-01 00:00:00
abstract：:Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induc...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0736-z
更新日期：2012-01-01 00:00:00
abstract：:Several lines of evidence suggest that the ubiquitin-proteasome pathway is involved in sepsis-induced muscle catabolism. The gene expression of ubiquitin and several of the proteasome subunits was increased in muscle from both septic rats and patients. In other studies, the activity of isolated 20S proteasomes was sti...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1023/a:1006916206260
更新日期：1999-04-01 00:00:00
abstract：:This study was designed to examine the interactions of ergosterol with bovine serum albumin (BSA) and human serum albumin (HSA) under physiological conditions with the drug concentrations in the range of 2.99-105.88 μM and the concentration of proteins was fixed at 5.0 μM. The analysis of emission spectra quenching at...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1814-6
更新日期：2012-10-01 00:00:00
abstract：:Recently, differentiated somatic cells had been reprogrammed to pluripotential state in vitro, and various tissue cells had been elicited from those cells. Epigenetic modifications allow differentiated cells to perpetuate the molecular memory needed for the cells to retain their identity. DNA methylation and histone d...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9489-3
更新日期：2010-03-01 00:00:00
abstract：:To investigate the influence of NF-κB antisense oligonucleotide on transdifferentiation of fibroblast in the pathological process of bleomycin-induced pulmonary fibrosis in mice. 6 h before molding of C57BL/6 model of pulmonary fibrosis in mice, NF-κB antisense oligonucleotide was injected from caudal vein. Then the l...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3273-8
更新日期：2014-06-01 00:00:00
abstract：:Mouse DNA and chromatin were melted on hydroxyapatite and the denaturation profiles of ribosomal and satellite DNAs were followed by hybridization with their complementary RNAs. Neither ribosomal nor bulk DNA had significantly different melting profiles in chromatin as compared to DNA. However, most of satellite DNA e...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00368300
更新日期：1977-06-01 00:00:00
abstract：:Groups of CBA mice were administered [35S] methionine (1 mCi/mouse). Non-histone proteins, H1 and H1(0) histones and nucleosomal core histones were isolated from different issues by selective extractions. The measurements of radioactivity of individual bands and autoradiography of dry gels were used to identify methio...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00444310
更新日期：1988-01-01 00:00:00
abstract：:Growth hormone-releasing hormone receptor (GHRHR) has important functions in the regulation of the growth hormone axis and the development and proliferation of pituitary somatotropes. Moreover, some mutations in mouse GHRHR can induce the dwarfism. The objective of this paper is to reveal the association of GHRHR with...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1927-y
更新日期：2012-12-01 00:00:00
abstract：:Oxidative stress plays a major role in myocardial injury. Morin, a bioflavonoid has known to possess various biological activities in previous studies. Hence, this study evaluated the cardioprotective mechanism(s) of Morin against isoproterenol induced myocardial necrosis in rats. Male albino Wistar rats were divided ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-04575-9
更新日期：2019-02-01 00:00:00
abstract：:Human type II inosine monophosphate dehydrogenase (IMPDH2) is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. Several Single Nucleotide Polymorphisms (SNP) affecting the IMPDH2 gene sequence have been reported with potent...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0668-z
更新日期：2011-11-01 00:00:00
abstract：AIM:To study the function of the prodomain of ADAM17 (TACE) and to develop an approach for interfering with inflammation processes. METHOD:The expression plasmids of the TACE ectodomain (T1300), prodomain (T591), signal peptide and prodomain (T648), full length (T2472), and the turncated TACE without prodomain (T57-T1...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9224-5
更新日期：2009-04-01 00:00:00
abstract：:The full-length MECPS cDNA sequence (designated as Chmecps, GenBank Accession No.: DQ415658) was isolated by rapid amplification of cDNA ends (RACE) for the first time from Cephalotaxus harringtonia. The full-length cDNA of Chmecps was 1,146 bp containing a 753 bp open reading frame (ORF) encoding a polypeptide of 250...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9377-2
更新日期：2009-09-01 00:00:00
abstract：:Laparoscopic surgery techniques have been increasingly preferred to classic laparotomy by surgeons since 1987. However, this method may have some side effects on different intraabdominal organs including kidneys. The aim of this study is to evaluate the effects of different pressures of CO2 on p53 related apoptosis in...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3519-5
更新日期：2014-10-01 00:00:00
abstract：:The influence of peptidyl-tRNA on the dissociation of yeast 80 S ribosomes into subunits was studied. For this purpose temperature-sensitive (ts) suppressor strain of yeast Saccharomyces cervisiae carrying a defect in peptide chain termination was used. It was found that peptidyl-tRNA did not influence the dissociatio...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00775964
更新日期：1978-06-16 00:00:00
abstract：:Spermiogenesis is a developmental process undergoing continuous differentiation to drive a diploid spermatogonium towards a haploid sperm cell. This striking transformation from spermatogonium to spermatozoa is made possible by the stage-specific adaption of cytoskeleton and associated molecular motor proteins. KIFC1 ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1593-0
更新日期：2012-07-01 00:00:00
abstract：:Uterine leiomyomas (UL) are prevalent benign tumors, especially among women of African ancestry. The disease also has genetic liability and is influenced by risk factors such as hormones and obesity. This study investigates the haplotypes of the Cytochrome P450 1B1 gene (CYP1B1) related to hormones and coiled-coil dom...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04790-y
更新日期：2019-06-01 00:00:00
abstract：:Fluorescent quantum dots (QDs) have been widely applied in biological and biomedical areas, but relatively little is known about the interaction of QDs with some natural enzymes. Herein, the interactions between 3-mercaptopropionic acid-capped CdTe QDs (MPA-QDs) and papain were systematically investigated by UV-Vis ab...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2681-5
更新日期：2013-10-01 00:00:00
abstract：:In the published original version, the complete list of authors was omitted. The complete list of contributing authors is updated with this Correction. ...
journal_title：Molecular biology reports
pub_type： 已发布勘误
doi：10.1007/s11033-018-4454-7
更新日期：2018-12-01 00:00:00
abstract：:Severe malaria (SM) caused by Plasmodium falciparum (Pf) infection has been associated with life-threatening anemia, metabolic acidosis, cerebral malaria and multiorgan dysfunction. It may lead to death if not treated promptly. RNASE 3 has been linked to Pf growth inhibition and its polymorphisms found associated with...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05934-1
更新日期：2020-11-01 00:00:00
abstract：:Cell free protein synthesis has become a powerful method for the high-throughput production of proteins that are difficult to express in living cells. The protein SAP2 of Fasciola hepatica (FhSAP2), which has demonstrated to be both, an excellent vaccine candidate against experimental fascioliasis and a good antigen f...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4251-3
更新日期：2018-10-01 00:00:00
abstract：:Although it is known that the hybrid male mouse is sterile just like any other animal's heterogametic sex, the reason why only the male germ cells are impaired has yet to be discovered. TdT-mediated dUTP nick end labeling assay using a confocal fluorescence microscope and DNA fragmentation assay of hybrid testis indic...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9321-5
更新日期：2009-07-01 00:00:00
