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Identification of minor tightly bound H1 histone subfractions which fail to cleave their initiator methionine.

Abstract：

:Groups of CBA mice were administered [35S] methionine (1 mCi/mouse). Non-histone proteins, H1 and H1(0) histones and nucleosomal core histones were isolated from different issues by selective extractions. The measurements of radioactivity of individual bands and autoradiography of dry gels were used to identify methionine-containing and methionine-free histone variants. H1A and H1B histone variants extracted with 5% perchloric acid were methionine-free. However, minor sub-fractions of these histones which are more tightly bound to DNA (and which can be extracted only with 0.25 N HCl) contained [35S] methionine and did show a higher specific activity than methionine-containing nucleosomal hitones. Cyanogen Bromide reaction which destroys non-histone proteins and methionine-containing nucleosomal histones removes radioactivity but does not alter the position of methionine-containing H1 minor bands. This indicates that the radioactive methionine occupies only the N-terminus of the H1 molecules. It is suggested that this methionine is an uncleaved initiator methionine. The presence of these methionine-containing minor H1 subfractions varies in different tissues.

journal_name

Mol Biol Rep

journal_title

Molecular biology reports

authors

Medvedev ZA,Medvedeva MN

doi

10.1007/BF00444310

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

145-9

issue

3

eissn

0301-4851

issn

1573-4978

journal_volume

13

pub_type

杂志文章

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