Soluble expression and purification of Brucella cell surface protein (BCSP31) of Brucella melitensis and preparation of anti-BCSP31 monoclonal antibodies.

abstract：:A cDNA encoding an O-methyltransferase (namely FGCOMT1) was identified from the medicinal plant Trigonella foenum-graecum L. The FGCOMT1 enzyme is a functional caffeic acid O-methyltransferase (COMT) and is localized in the cytosol. Kinetic analysis indicated that FGCOMT1 protein exhibited the highest catalyzing effic...

journal_title：Molecular biology reports

authors： Qin JC,Zhang YM,Lang CY,Yao YH,Pan HY,Li X

更新日期：2012-02-01 00:00:00

abstract：:The phosphoenolpyruvate (PEP) carboxylase is regulated at the levels of transcription and post-translation in C4 plants in light and abundantly accumulates in leaf mesophyll cells. We report here developmental and photosynthetic regulation of stably accumulated Bacillus thuringiensis δ-endotoxin under the control of P...

journal_title：Molecular biology reports

authors： Khan MS,Ali S,Iqbal J

更新日期：2011-04-01 00:00:00

abstract：:CENP-A locates at nucleosome as histone H3-like proteins, and is phosphorylated during mitosis. We investigated the dynamic distribution of p-CENP-A to explore the details of its function. We found that p-CENP-A was phosphorylated at late prophase, and the signal of p-CENP-A arranged at equatorial plate along with nuc...

journal_title：Molecular biology reports

authors： Li D,Liu R,Song L,Zhou H,Chen J,Huang X

更新日期：2008-12-01 00:00:00

abstract：:De novo assemblies of transcriptome sequence of F(1) interspecies hybrids between Mandarin fish Siniperca chuatsi (Basilewsky) (♀) and Golden mandarin fish Siniperca scherzeri Steindachner (♂) were generated using Illumina sequencing. In the present study, 64 microsatellite primer pairs were designed on basis of the t...

journal_title：Molecular biology reports

authors： Huang W,Liang X,Qu C,Cao J,Zhao C,Cao L

更新日期：2013-02-01 00:00:00

abstract：:The widely studied candidate genes of the renin-angiotensin-aldosterone system, angiotensinogen (AGT), and angiotensin II receptor type 1 (AGTR1), are implicated in the development of diabetic nephropathy (DN). A number of studies have evaluated the association between the functional polymorphisms, AGT M235T and AGTR1...

journal_title：Molecular biology reports

authors： Ding W,Wang F,Fang Q,Zhang M,Chen J,Gu Y

更新日期：2012-02-01 00:00:00

abstract：:Vitiligo is an acquired pigmentary disorder characterized by loss of epidermal melanocytes. A strong association at a single nucleotide polymorphism (SNP) rs11966200 within MHC region had been identified in a recent genome-wide association study of generalized vitiligo in Chinese Han population. This study aims to inv...

journal_title：Molecular biology reports

authors： Tang J,Liu JL,Zhang C,Hu DY,He SM,Zuo XB,Wang PG,Sun LD,Zhang XJ,Yang S

更新日期：2013-06-01 00:00:00

abstract：:Alterations in expression of the DFF40 gene have been reported in some cancers. This study is an in vitro study of the therapeutic effects of gene transfer that lead to elevation in DFF40 expression within T-47D cells in the presence of sulfonamide drugs. In this study, we have constructed a eukaryotic expression vect...

journal_title：Molecular biology reports

authors： Bagheri F,Safarian S,Eslaminejad MB,Sheibani N

更新日期：2014-11-01 00:00:00

abstract：:The water-soluble group B3 vitamin nicotinamide (NAM) is involved in a wide range of physical processes through biosynthetically converted to nicotinamide adenine dinucleotide (NAD(+)). In addition to its pivotal role in energy metabolism, NAD(+) is also the indispensable substrate of poly (ADP-ribose) polymerase-1 (P...

journal_title：Molecular biology reports

authors： Zhang XM,Jing YP,Jia MY,Zhang L

更新日期：2012-12-01 00:00:00

abstract：:RNA-protein interaction in the 30S subunits of rat liver hnRNP has been studied by crosslinking of informofer proteins to hnRNA induced by UV irradiation. Irradiation of 30S particles with 254 nm UV light in doses of 1 1 x 10(5) erg/mm2 leads to the extensive crosslinking hnRNA to informofer proteins. The crosslinked ...

journal_title：Molecular biology reports

authors： Marczinovits I,Molnár J

更新日期：1982-03-31 00:00:00

abstract：:Growth-regulating factor (GRF) is an important protein in GA-mediated response, with key roles in plant growth and development. However, it is not known whether or how the GRF proteins in citrus to regulate organ size. In this study, nine citrus GRF genes (CsGRF1-9) were validated from the 'Anliu' sweet orange (AL, Ci...

journal_title：Molecular biology reports

authors： Liu X,Guo LX,Jin LF,Liu YZ,Liu T,Fan YH,Peng SA

更新日期：2016-10-01 00:00:00

abstract：:Lung cancer is the most common cause of cancer death worldwide. Smoking is known as the strongest single factor in the development of lung cancer. However, there are inherited genetic factors that cause different responses to cigarette smoking exposure among individuals. We tried to identify these differences in heavy...

journal_title：Molecular biology reports

authors： Lee M,Lee Y,Cho HJ,Hong J,Kwon SJ,Park CG,Lee HY,Son JW,Kang J

更新日期：2011-11-01 00:00:00

abstract：:ERBB2/HER2/NEU, a member of the epidermal growth factor receptor family, is overexpressed in more than 25 % of non-small cell lung cancer and is considered to be a significant and independent prognostic factor in lung cancer. Here we generated a lung specific HER2 overexpressing transgenic mouse model. In this model, ...

journal_title：Molecular biology reports

authors： Zeng S,Yang Y,Tan Y,Lu C,Pan Y,Chen L,Lu G

更新日期：2012-08-01 00:00:00

abstract：:The disturbed immune homeostasis is involved in the pathogenesis of an array of autoimmune diseases like rheumatoid arthritis (RA). The adenosine monophosphate-activated protein kinase (AMPK) with a pivotal role in immunometabolism process, also plays a regulatory function in the immune system. This study aims to eval...

journal_title：Molecular biology reports

authors： Samimi Z,Kardideh B,Zafari P,Bahrehmand F,Roghani SA,Taghadosi M

更新日期：2019-12-01 00:00:00

abstract：:Recently, differentiated somatic cells had been reprogrammed to pluripotential state in vitro, and various tissue cells had been elicited from those cells. Epigenetic modifications allow differentiated cells to perpetuate the molecular memory needed for the cells to retain their identity. DNA methylation and histone d...

journal_title：Molecular biology reports

authors： Zhang XM,Li QM,Su DJ,Wang N,Shan ZY,Jin LH,Lei L

更新日期：2010-03-01 00:00:00

abstract：:The present meta-analysis of relevant case-control studies was conducted to investigate the possible relationships between genetic variations in the killer cell immunoglobulin-like receptor (KIR) gene clusters of the human KIR gene family and susceptibility to ankylosing spondylitis (AS). The following electronic data...

journal_title：Molecular biology reports

authors： Zuo HN,Wang ZL,Cui DR,Xin DJ

更新日期：2014-08-01 00:00:00

abstract：:Chronic obstructive pulmonary disease (COPD) is a complex human disease influenced by multiple genes and environmental factors. The SERPINE2 gene has recently been demonstrated to be associated with COPD onset in a non-East Asian population. In this study, we genotyped 20 single nucleotide polymorphisms (SNPs) in SERP...

journal_title：Molecular biology reports

authors： An L,Yang T,Zhang Y,Lin Y,Zhang H,Jiao X,Hua L,Dai H,Wang C

更新日期：2012-02-01 00:00:00

abstract：:The genes of E. coli are located on a circular chromosome of 4.6 million basepairs. This 1.6 mm long molecule is compressed into a nucleoid to fit inside the 1-2 microm cell in a functional format. To examine the role of DNA supercoiling as nucleoid compaction force we modulated the activity of DNA gyrase by electroni...

journal_title：Molecular biology reports

authors： Stuger R,Woldringh CL,van der Weijden CC,Vischer NO,Bakker BM,van Spanning RJ,Snoep JL,Westerhoff HV

更新日期：2002-01-01 00:00:00

abstract：:The interleukin (IL)-1β-511 C/T polymorphism has been shown to be functional and to contribute to the risk of gastric cancer. However, the relationship between the IL-1β-511 C/T polymorphism and gastric carcinogenesis remains inconclusive. A systematical electronic search was conducted of the MEDLINE, EMBASE, and CENT...

journal_title：Molecular biology reports

authors： Park MJ,Hyun MH,Yang JP,Yoon JM,Park S

更新日期：2015-01-01 00:00:00

abstract：:Current study was conducted to investigation of effect of magnetic field on cell dedifferentiation and follow it callus induction derived from mature embryo culture in bread wheat genotypes. For this purpose, a factorial experiment based on completely randomized design was carried out with two wheat genotypes and thre...

journal_title：Molecular biology reports

authors： Kahrizi D,Cheghamirza K,Akbari L,Rostami-Ahmadvandi H

更新日期：2013-02-01 00:00:00

abstract：:Human Wharton's jelly mesenchymal stem cells (hWJSCs) are multipotent stem cells that could be aggregated into 3D spherules. ITGA4 and ITGA5 genes encode α4 and α5 subunits of integrins, respectively. In this study, we analyzed expression levels of ITGA4 and ITGA5 gene mRNAs in undifferentiated and 3D spherules formin...

journal_title：Molecular biology reports

authors： Mostafavi-Pour Z,Ashrafi MR,Talaei-Khozani T

更新日期：2018-06-01 00:00:00

abstract：:We utilized Percoll density gradient centrifugation to isolate and fractionate chloroplasts of Korean winter wheat cultivar cv. Kumgang (Triticum aestivum L.). The resulting protein fractions were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE) coupled with LTQ-FTICR mass spectrometry. This e...

journal_title：Molecular biology reports

authors： Kamal AH,Cho K,Komatsu S,Uozumi N,Choi JS,Woo SH

更新日期：2012-05-01 00:00:00

abstract：:Micro-RNAs (miRNAs) play key roles in regulating genes of the immune system. The aim of this study was to examine the expression of miR-1, 21 and 125a in the immune cells taken from the peripheral blood of patients suffering from chronic HBV infection (CHB). This cross-sectional study was performed on 60 CHB patients ...

journal_title：Molecular biology reports

authors： Momeni M,Hassanshahi G,Arababadi MK,Kennedy D

更新日期：2014-07-01 00:00:00

abstract：:A number of regions designated as RD1-RD16 (region of difference) and encompassing 129 open reading frames have been identified between Mycobacterium tuberculosis and Mycobacterium bovis on the one hand and Bacillus Calmette-Guérin on the other. Identification of T cell epitopes from this set of proteins may serve to ...

journal_title：Molecular biology reports

authors： Wang J,Zhang H,Wang H

更新日期：2010-04-01 00:00:00

abstract：:Adequate metabolic adaptation of key tissues playing an essential role for bioenergetic homeostasis and lactogenesis is critical in cows to adapt to changes in energy requirements and physiological processes during the lactation period. Mitochondria are recognized as central to meet energy needs and maintaining of met...

journal_title：Molecular biology reports

authors： Weikard R,Kuehn C

更新日期：2018-10-01 00:00:00

abstract：:Silky Fowl is a natural mutant with hyperpigmentation of various internal tissues. Although the mechanism of hyperpigmentation remains unclear, recent studies have shown that the abnormal migration of melanoblast and the absence of environmental barrier molecules are responsible for the hyperpigmentation in Silky Fowl...

journal_title：Molecular biology reports

authors： Li Y,Zhu X,Yang L,Li J,Lian Z,Li N,Deng X

更新日期：2011-02-01 00:00:00

abstract：:Methicillin resistant Staphylococcus aureus (MRSA) is one of the challenging bacterial pathogen due to its acquired resistance to the β lactam antibiotics. The Sortase A is an enzyme of Gram-positive bacteria including S. aureus to anchor surface proteins to the cell wall. Sortase A is well studied enzyme and consider...

journal_title：Molecular biology reports

authors： Uddin R,Saeed K

更新日期：2014-08-01 00:00:00

abstract：:Yellow lupin seeds (Lupinus luteus) cytoplasmic tRNAGlu was isolated and the primary structure was determined to be: (sequence in text) AGU CCCGGCGACGGAACCAOH. It is 76 nucleotides long and contains 8 modified nucleosides: 2 residues of pseudouridine, ribothymidine, 3 dihydrouridines, 5-methylcytosine and 1-methyladen...

journal_title：Molecular biology reports

authors： Barciszewska M,Barciszewski J

更新日期：1988-01-01 00:00:00

abstract：:Pathologic complete response after neoadjuvant systemic treatment appears to be a valid surrogate for better overall survival in breast cancer patients. Currently, together with standard clinicopathologic assessment, novel molecular biomarkers are being exhaustively tested in order to look into the heterogeneity of br...

journal_title：Molecular biology reports

authors： Kolacinska A,Fendler W,Szemraj J,Szymanska B,Borowska-Garganisz E,Nowik M,Chalubinska J,Kubiak R,Pawlowska Z,Blasinska-Morawiec M,Potemski P,Jeziorski A,Morawiec Z

更新日期：2012-07-01 00:00:00

abstract：:A pattern recognition protein (PRP), lipopolysaccharide and beta-1,3-glucan binding protein (LGBP) cDNA was cloned from the haemocyte of Chinese shrimp Fenneropenaeus chinensis by the techniques of homology cloning and RACE. Analysis of nucleotide sequence revealed that the full-length cDNA of 1,275 bp has an open rea...

journal_title：Molecular biology reports

authors： Liu F,Li F,Dong B,Wang X,Xiang J

更新日期：2009-03-01 00:00:00

abstract：:70S RNA has been prepared from mouse mammary tumor virus (MMTV) produced by the GR tumor cell line. After denaturation for 3 min at 60 degrees C the RNA was applied to a sucrose gradient and molecules sedimenting between 10-16S were selected and passed over an oligo-dT cellulose column. The poly A containing RNA fract...

journal_title：Molecular biology reports

authors： Klemenz R,Reinhardt M,Diggelmann H

更新日期：1981-05-22 00:00:00