Abstract:
:The human dopamine transporter gene (hDAT) encodes the dopamine transporter in dopamine (DA) neurons to regulate DA transmission. hDAT expression varies significantly from neuron to neuron, and from individual to individual so that dysregulation of hDAT is related to many neuropsychiatric disorders. It is critical to identify hDAT-specific cis-acting elements that regulate the hDAT expression. Previous studies showed that hDAT Intron 1 displayed inhibitory activity for reporter gene expression. Here we report that the hDAT Intron 1 contains a 121-bp fragment that down-regulated both SV40 and hDAT promoter activities by 80% in vitro. Subfragments of 121-bp still down-regulated the SV40 promoter but not the hDAT promoter, as supported by nuclear protein-binding activities. Collectively, 121-bp is a silencer in vitro that might coordinate with transcriptional activities both inside and outside 121-bp in regulation of hDAT.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Zhao Y,Zhou Y,Xiong N,Lin Zdoi
10.1007/s11033-011-1339-4subject
Has Abstractpub_date
2012-05-01 00:00:00pages
5393-9issue
5eissn
0301-4851issn
1573-4978journal_volume
39pub_type
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