Spontaneous uptake of exogenous DNA by goat spermatozoa and selection of donor bucks for sperm-mediated gene transfer.

Abstract:

:Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper alternative to more commonly used methods of producing transgenic animals. In this study, the capra semen ejaculates were pooled together and then incubated in vitro with DIG-labeled DNA. The binding and internalizing rates were observed by the in situ hybridization methods. We also compared the standard sperm parameters and the efficiencies of interaction with exogenous DNA of 60 individuals to select donor bucks for SMGT. It was showed that labeled exogenous DNA was detected in different localizations in spermatozoa but genuine DNA uptake, in contrast to mere binding, seems to be limited to the postacrosomal region. The removal of seminal plasma increased significantly (P < 0.01) the capability in picking up exogenous DNA. Use of frozen-thawed semen (without cryoprotectant agents) and Triton X-100 treatment also increased significantly (P < 0.01) the DNA-binding capacity, but reduced the sperm viability. The binding rates (the proportion of labeled-DNA positive spermatozoa to all the spermatozoa counted) of 60 buck individuals were in the range of 3.08-73.39%, and the internalizing rates (the proportion of DNaseI-treated labeled-DNA positive spermatozoa to all the spermatozoa counted) were 4.83-70.00%. About 8.34% (5/60) bucks showed high binding, but low internalizing ability. Chi-square test showed that there was significant difference among the breeds (x(2) = 26.515, P < 0.01). Eight individual bucks that demonstrated high DNA uptake were selected for SMGT. It was demonstrated that the goat spermatozoa was capable of spontaneous uptake of exogenous DNA. Seminal fluid inhibits DNA uptake and that membrane disruption increases DNA binding but greatly diminishes uptake.

journal_name

Mol Biol Rep

authors

Zhao Y,Yu M,Wang L,Li Y,Fan J,Yang Q,Jin Y

doi

10.1007/s11033-011-1019-4

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

2659-64

issue

3

eissn

0301-4851

issn

1573-4978

journal_volume

39

pub_type

杂志文章
  • Methyl jasmonate a stress phytohormone attenuates LPS induced in vivo and in vitro arthritis.

    abstract::The objective of present study was to screen the effect of methyl jasmonate (MJ) in lipopolysaccharide (LPS) induced in vivo and in vitro arthritis. Arthritis was induced in wistar rats by intraplantar administration of LPS (1 mg/Kg) and effect of MJ was screened in two doses (20, 40 mg/Kg, IP), indomethacin (30 mg/Kg...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4520-1

    authors: Gunjegaonkar SM,Shanmugarajan TS

    更新日期:2019-02-01 00:00:00

  • Potential protein partners for the N-terminal domain of human topoisomerase I revealed by phage display.

    abstract::Phage display procedure was applied to the N-terminal domain of human topoisomerase I. The consensus sequence identified for clones binding to the N-terminal domain was found in 35 human proteins that are either permanently or temporarily located in the nucleus. They are in majority involved in the DNA repair, transcr...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/a:1021237021338

    authors: Trzcińska AM,Girstun A,Piekiełko A,Kowalska-Loth B,Staroń K

    更新日期:2002-12-01 00:00:00

  • Hemp (Marijuana) reverted Copper-induced toxic effects on the essential fatty acid profile of Labeo rohita and Cirrhinus mrigala.

    abstract::Heavy metals pollution affects the nutritive value of fish. This study examined if the inclusion of dietary hempseed (HS) and hempseed oil (HO) in the diet of the fish could revert the copper-induced toxic effects on muscle fatty acid profile of rohu (Labeo rohita) and mrigal (Cirrhinus mrigala). Fingerlings of both s...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4483-2

    authors: Afridi AJ,Zuberi A,Yousafzai AM,Maria,Kamran M,Ullah S

    更新日期:2019-02-01 00:00:00

  • A folate receptor alpha double-mutated haplotype 1816delC-1841A is distributed throughout Eurasia and associated with lower erythrocyte folate levels.

    abstract::Folate is crucial for various cellular functions. Several transport mechanisms allow folate to enter the intracellular compartment with folate receptor-α being the major high-affinity receptor. Rare genetic variations in exons of the FR-α gene, FOLR1, were recently shown to cause severe folate deficiency accompanied b...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1236-x

    authors: Nilsson TK,Laanpere M,Altmäe S,Serra-Majem L,Salumets A

    更新日期:2012-04-01 00:00:00

  • PMS1 from Arabidopsis thaliana: optimization of protein overexpression in Escherichia coli.

    abstract::One of the major limitations when attempting to obtain detailed biochemical, biophysical and immunological characterization of plant DNA mismatch repair proteins is their extremely low abundance in vivo under normal growth conditions. An initial analysis of PMS1 transcript level in various Arabidopsis thaliana tissues...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0203-2

    authors: Galles C,Gomez RL,Spampinato CP

    更新日期:2011-02-01 00:00:00

  • Selection of reference genes for normalization of qRT-PCR analysis of differentially expressed genes in soybean exposed to cadmium.

    abstract::Accurate normalization of gene expression with qRT-PCR depends on the use of appropriate reference genes (RGs) for the species under a given set of experimental conditions. Multiple RGs for gene expression analysis of soybean exposed to heavy metal stress treatment have not been reported in the literature. In this stu...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-0897-9

    authors: Wang Y,Yu K,Poysa V,Shi C,Zhou Y

    更新日期:2012-02-01 00:00:00

  • Coenzyme Q10 in association with metabolism-related AMPK/PFKFB3 and angiogenic VEGF/VEGFR2 genes in breast cancer patients.

    abstract::Low levels of coenzyme Q10 (CoQ10) have been reported in the circulation of patients with breast cancer, particularly in metastatic features. Our objective was to study the correlation between plasma levels of CoQ10 and the tumoral expression levels of AMPK, PFKFB3, VEGF, and VEGFR2. This study was a part of consecuti...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05310-z

    authors: Abdi S,Montazeri V,Garjani A,Shayanfar A,Pirouzpanah S

    更新日期:2020-04-01 00:00:00

  • oxLDL inhibits differentiation of mesenchymal stem cells into osteoblasts via the CD36 mediated suppression of Wnt signaling pathway.

    abstract::Bone abnormalities as a consequence of osteoblast deregulation are associated with several diseases such as diabetes and chronic kidney disease. Important role for oxidized low density lipoproteins (oxLDL) in the pathophysiology of bone disorders has been reported. However, little is known about the effects and mechan...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04735-5

    authors: Dawodu D,Patecki M,Dumler I,Haller H,Kiyan Y

    更新日期:2019-06-01 00:00:00

  • Overexpressed RACK1 is positively correlated with malignant degree of human colorectal carcinoma.

    abstract::RACK1 is a crucial scaffold and anchoring protein, which plays a vital role in multiple signaling pathways of tumorigenesis. The aim of the present study was to identify the correlation between expressions of RACK1 and malignant degrees in colorectal carcinoma (CRC) patients. All together 157 CRC patients were enrolle...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3201-y

    authors: Jin S,Mu Y,Wang X,Liu Z,Wan L,Xiong Y,Zhang Y,Zhou L,Li L

    更新日期:2014-05-01 00:00:00

  • GSTP1 Ile105Val polymorphism is associated with lung cancer risk among Asian population and smokers: an updated meta-analysis.

    abstract::Many studies have examined the association between the GSTP1 Ile105Val (rs 1695) gene polymorphism and lung cancer risk in various populations, but their results have been inconsistent. To assess this relationship more precisely, a meta-analysis was performed. The PubMed and CNKI database was searched for case-control...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-014-3290-7

    authors: Xu CH,Wang Q,Zhan P,Qian Q,Yu LK

    更新日期:2014-07-01 00:00:00

  • DNA barcoding to characterize biodiversity of freshwater fishes of Egypt.

    abstract::The current study represents the first molecular characterization of freshwater fish species in Egypt from two major fish resources; the River Nile and Lake Nasser. A total of 160 DNA barcodes using a 655-bp-long fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene were generated from 37 species belon...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05657-3

    authors: Ali FS,Ismail M,Aly W

    更新日期:2020-08-01 00:00:00

  • Development of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts.

    abstract::Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4329-y

    authors: Ascari IJ,Martins SC,Camargo LSA,Mendez-Otero R,Jasmin

    更新日期:2018-12-01 00:00:00

  • Interleukin-8 and -10 gene polymorphisms in irritable bowel syndrome.

    abstract::Irritable bowel syndrome (IBS) is one of the most common gastrointestinal diagnoses seen by primary care providers and gastroenterologists. Proinflammatory cytokines interleukin (IL)-6 and IL-8 have been found increased in IBS patients. Cytokine gene single nucleotide polymorphisms (SNPs) of IL-8 and IL-10 have not be...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-1745-2

    authors: Romero-Valdovinos M,Gudiño-Ramírez A,Reyes-Gordillo J,Martínez-Flores WA,Ramírez-Miranda ME,Maravilla P,Olivo-Díaz A

    更新日期:2012-09-01 00:00:00

  • Highly efficient CRISPR-targeting of the murine Hipp11 intergenic region supports inducible human transgene expression.

    abstract::Safe harbor loci allow predicable integration of a transgene into the genome without perturbing endogenous gene activity and for decades have been exploited in the mouse to investigate gene function, generate humanised models and create tissue specific reporter and Cre recombinase expressing lines. Herein, we show tha...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05204-9

    authors: Browning J,Rooney M,Hams E,Takahashi S,Mizuno S,Sugiyama F,Fallon PG,Kelly VP

    更新日期:2020-02-01 00:00:00

  • Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line.

    abstract::The aim of the present study was construction of mammary gland specific expression vector for high level of human insulin (hINS) expression in transgenic buffalo for therapeutic use. We have constructed mammary gland specific vector containing human insulin gene and there expression efficiency was checked into in vitr...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3464-3

    authors: Kaushik R,Singh KP,Kumari A,Rameshbabu K,Singh MK,Manik RS,Palta P,Singla SK,Chauhan MS

    更新日期:2014-09-01 00:00:00

  • Cloning and expression of MyoG gene from Hu sheep and identification of its myogenic specificity.

    abstract::This experiment was conducted to explore the biological functions of myogenin (MyoG) gene. MyoG gene was cloned from genome of Hu sheep by overlap extension PCR. Then, pEGFP-C1-MyoG and pcDNA3.0-MyoG fusion expression vectors was constructed and pEGFP-C1-MyoG vector had been transfected into NIH-3T3 cells by liposomes...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2945-0

    authors: Zhang Z,Xu F,Zhang Y,Li W,Yin Y,Zhu C,Du L,Elsayed AK,Li B

    更新日期:2014-02-01 00:00:00

  • A comparative study on the two classes of heterogeneous nuclear ribonucleoprotein particles separated in metrizamide density gradient, by electrophoresis of proteins and chase experiments. Evidence for two distinct subfractions of HnRNP in mammalian nucle

    abstract::Heterogeneous nuclear ribonucleoprotein particles (HnRNP) were separated in metrizamide density gradients, into two fractions migrating to 1.31 g ml-1 and 1.18 g ml-1, respectively. Proteins associated with each of these fractions were analysed by SDS-acrylamide gel electrophoresis. It is shown that the whole proteins...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00643481

    authors: Houssais JF

    更新日期:1977-03-01 00:00:00

  • Gallic and vanillic acid suppress inflammation and promote myelination in an in vitro mouse model of neurodegeneration.

    abstract::Neuroinflammation affects millions of people around the world as a result of injury or stress. Neuroinflammation represents almost all types of neurological diseases such as multiple sclerosis and Alzheimer's disease. Neurodegenerative diseases comprise demyelination and synaptic loss. The inflammatory response is fur...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4557-1

    authors: Siddiqui S,Kamal A,Khan F,Jamali KS,Saify ZS

    更新日期:2019-02-01 00:00:00

  • Identification of differentially expressed genes under heat stress conditions in rice (Oryza sativa L.).

    abstract::Rice production in recent years is highly affected by rapidly increasing temperatures in the tropical and sub-tropical countries, which threatens the sustainable production in near future. Hence, understanding the heat tolerance mechanism and evolving tolerant varieties is an immense need in the staple crop rice. An e...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05291-z

    authors: Wahab MMS,Akkareddy S,Shanthi P,Latha P

    更新日期:2020-03-01 00:00:00

  • Characterization of HbEREBP1, a wound-responsive transcription factor gene in laticifers of Hevea brasiliensis Muell. Arg.

    abstract::AP2/ERF transcription factors play an important role in regulation of the cross-talk between ethylene and jasmonate signaling pathways mediating defense responses of plants to biotic and abiotic stresses. In this study, an AP2/ERF transcription factor gene was isolated and characterized from laticifers of rubber tree ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1146-y

    authors: Chen YY,Wang LF,Dai LJ,Yang SG,Tian WM

    更新日期:2012-04-01 00:00:00

  • Molecular cloning and functional expression analysis of a new gene encoding geranylgeranyl diphosphate synthase from hazel (Corylus avellana L. Gasaway).

    abstract::Geranylgeranyl diphosphate synthase (GGPPS) [EC 2.5.1.29] catalyzes the biosynthesis of geranylgeranyl diphosphate (GGPP), which is a key precursor for diterpenes such as taxol. Herein, a full-length cDNA encoding GGPPS (designated as CgGGPPS) was cloned and characterized from hazel (Corylus avellana L. Gasaway), a ta...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-009-9935-2

    authors: Wang Y,Miao Z,Tang K

    更新日期:2010-10-01 00:00:00

  • Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor.

    abstract::Latexin, a carboxypeptidase A inhibitor, is expressed in a cell type-specific manner in both central and peripheral nervous systems in the rat. It is used as a molecular marker for the regional specification of the neocortex. In this study, a cDNA was isolated from a human fetal brain cDNA library. The cDNA (LXN) cont...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/a:1010971219806

    authors: Liu Q,Yu L,Gao J,Fu Q,Zhang J,Zhang P,Chen J,Zhao S

    更新日期:2000-01-01 00:00:00

  • The apoptosis inhibitor survivin prevents insect midgut from cell death during postembryonic development.

    abstract::The programmed cell death (PCD) is important in maintaining the cell number homoeostasis of tissues and organs in metazoan. This process is regulated by the inhibitors of apoptosis proteins (IAPs). The function and mechanism of IAPs have been well studied in animal embryonic development and human cancers. However, the...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-0909-9

    authors: He HJ,Hou L,Wang JX,Zhao XF

    更新日期:2012-02-01 00:00:00

  • Protein biosynthesis in isolated human scalp hair follicles.

    abstract::The present study demonstrates that protein biosynthesis can be studied in single isolated human scalp hair follicles. The matrix and the sheath are the main regions where amino acids are built in. Incorporation is linear for at least five hours. The newly synthesized proteins can be separated into a water-soluble, a ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00777556

    authors: Vermorken AJ,Weterings PJ,Bloemendal H

    更新日期:1979-02-15 00:00:00

  • Analysis of predicted CD8+ T cell epitopes from proteins encoded by the specific RD regions of Mycobacterium tuberculosis for vaccine development and specific diagnosis.

    abstract::A number of regions designated as RD1-RD16 (region of difference) and encompassing 129 open reading frames have been identified between Mycobacterium tuberculosis and Mycobacterium bovis on the one hand and Bacillus Calmette-Guérin on the other. Identification of T cell epitopes from this set of proteins may serve to ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-009-9613-4

    authors: Wang J,Zhang H,Wang H

    更新日期:2010-04-01 00:00:00

  • Validation of a one-step PCR assay for the molecular identification of Echinococcus granulosus sensu stricto G1-G3 genotype.

    abstract::The Italian National Reference Center for Echinococcosis (CeNRE, Sassari, Italy) set up a diagnostic protocol of "one-step-PCR" useful for the detection of E. granulosus sensu stricto (E.g.s.s.) and the identification of its genotype (G1-G3). The purpose of this work was to perform the validation of the "PCR E.g.s.s."...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04625-w

    authors: Santucciu C,Masu G,Mura A,Peruzzu A,Piseddu T,Bonelli P,Masala G

    更新日期:2019-04-01 00:00:00

  • Efficiency of RAPD, SSR and cytochrome P450 gene based markers in accessing genetic variability amongst finger millet (Eleusine coracana) accessions.

    abstract::Finger millet (Eleusine coracana L.) is an important crop used for food, forage, and industrial products. Three DNA marker techniques, random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and cytochrome P(450) gene based markers were used for the detection of genetic polymorphism in 83 accessions of f...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0067-5

    authors: Panwar P,Saini RK,Sharma N,Yadav D,Kumar A

    更新日期:2010-12-01 00:00:00

  • Genetic divergence in wild population of Labeo rohita (Hamilton, 1822) from nine Indian rivers, analyzed through MtDNA cytochrome b region.

    abstract::The present study examined partial cytochrome b gene sequence of mitochondrial DNA for polymorphism and its suitability to determine the genetic differentiation in wild Labeo rohita. The 146 samples of L. rohita were collected from nine distant rivers; Satluj, Brahmaputra, Son, Chambal Mahanadi, Rapti, Chauka, Bhagira...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1140-4

    authors: Luhariya RK,Lal KK,Singh RK,Mohindra V,Punia P,Chauhan UK,Gupta A,Lakra WS

    更新日期:2012-04-01 00:00:00

  • Lithium facilitates removal of misfolded proteins and attenuated faulty interaction between mutant SOD1 and p-CREB (Ser133) through enhanced autophagy in mutant hSOD1G93A transfected neuronal cell lines.

    abstract::Abnormally protein aggregation and deposition are key pathological features of ALS, which may related with dysfunctional cellular autophagy. In the current study, we found that, compared with wtSOD1 cells, serum starvation treatment resulted in significant higher percentage of apoptosis in mutSOD1 cells; Lithium treat...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05071-4

    authors: Yin X,Wang S,Wang X,Yang Y,Jiang H,Wang T,Wang Y,Zhang C,Feng H

    更新日期:2019-12-01 00:00:00

  • Single nucleotide polymorphism in the STAT5b gene is associated with body weight and reproductive traits of the Jinghai Yellow chicken.

    abstract::In our research, signal transducer and activator of transcription 5b (STAT5b) gene was studied as candidate gene associated with body weight and reproductive traits of Jinghai Yellow chicken. Single nucleotide polymorphisms (SNPs) of STAT5b gene were examined in both Jinghai Yellow chicken and three reference chicken ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1202-7

    authors: Zhao XH,Wang JY,Zhang GX,Wei Y,Gu YP,Yu YB

    更新日期:2012-04-01 00:00:00