Abstract:
:Experiments with 5'-azacytidine and hematopoietic growth factor approved for the transformation of human mesenchymal cells into hematopoietic cells have demonstrated that cell fate can be dramatically altered by changing the epigenetic state of cells. Here, we demonstrate that umbilical cord-derived human mesenchymal stem cells (uMSC) are easily accessible and could be induced into cells with hematopoietic function. Furthermore, we focused on the crucial miRNAs and relative transcription factors (TFs) in our study. We show that combined Aza/GF incubation can increase expression of miR-218, miR-150, and miR-451. Accordingly, miR-218 overexpression achieved an increase in expression of CD34 (3-13%), CD45 (50-65%), CD133 and c-Kit in uMSCs that cultured with Aza/GF. The expression of the relevant transcriptional factors, such as HoxB4 and NF-Ya, was higher than in the negative control group or miR-218 inhibitor transfected group, and microphthalmia-associated transcription factor (MITF) is regarded to be a direct target of miR-218, as demonstrated by luciferase assays. Overexpression of miR-218 might, in conjunction with the MITF, upregulate the expression of NF-Ya and HoxB4, which induce a hematopoietic state. We concluded that miR-218 might have a role in the transformation of hematopoietic cells through the MITF pathway.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Hu K,Xu C,Ni H,Xu Z,Wang Y,Xu S,Ji K,Xiong J,Liu Hdoi
10.1007/s11033-014-3351-ysubject
Has Abstractpub_date
2014-07-01 00:00:00pages
4803-16issue
7eissn
0301-4851issn
1573-4978journal_volume
41pub_type
杂志文章abstract::Ebselen, an organoselenium compound, mimics glutathione peroxidase activity. It is a multifunctional compound, which catalyzes several essential reactions for the protection of cellular components from oxidative and free radical damage. Based on a number of in vitro and in vivo studies, various mechanisms are proposed...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-014-3417-x
更新日期:2014-08-01 00:00:00
abstract::Local domestic chicken populations are of paramount importance as a source of protein in developing countries. Bangladesh possesses a large number of native chicken populations which display a broad range of phenotypes well adapted to the extreme wet and hot environments of this region. This and the fact that wild jun...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2522-6
更新日期:2013-06-01 00:00:00
abstract::Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that plays an important role in breast cancer pathophysiology and risk definition, since it regulates the bioavailable fraction of circulating estradiol. Epidemiological studies have evaluated the association between SHBG Asp327Asn polymorphism and breast ca...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-1680-2
更新日期:2012-08-01 00:00:00
abstract::Experimental evidence highlights the importance of dietetic factors on breast cancer. In this work we aimed to analyze the effects two oils, corn oil (rich in n-6 polyunsaturated fatty acids -PUFA-) and extra virgin olive oil (EVOO), on oxidative stress in an animal model of breast carcinogenesis. Female rats were fed...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05492-6
更新日期:2020-07-01 00:00:00
abstract::This study aims to investigate the apoptotic and anti-angiogenic effect of Zoledronic acid on hormone-refractory prostate cancer cell lines. XTT cell proliferation assay used to assess cytotoxicity. Caspase 3/7 activity and DNA fragmentation were measured to verify apoptosis. Angiogenic cytokine profiles investigated ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05840-6
更新日期:2020-10-01 00:00:00
abstract::ATP-binding cassette transporter A1 (ABCA1) modulates plasma levels of high density lipoprotein (HDL), a cardiovascular protecting factor. Tree shrew was considered to be an animal protected from atherosclerosis characterized by high proportion of HDL in plasma. The cDNA clones and expression of tree shrew ABCA1 was i...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9199-7
更新日期:2009-03-01 00:00:00
abstract::Yangyin Tongnao granules (YYTNG) have been extensively applied in the treatment of brain injury, mainly due to its antioxidant effects, inhibition of apoptosis, and enhancement of blood circulation. To analyze the effect of YYTNG on the recovery of neurological function and neurogenesis in the peri-infarct area after ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04824-5
更新日期:2019-08-01 00:00:00
abstract::STAT4 is a transcription factor that has been implicated in the pathogenesis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Recently, several reports has documented that a STAT4 haplotype is associated with RA, SLE and Sjogren's syndrome. To summarize and review these findings, we conducted a met...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-009-9553-z
更新日期:2010-01-01 00:00:00
abstract::Complexity is one of the hallmarks that applies to C2H2 type zinc finger proteins (ZFPs). Structurally distinct clusters of zinc finger modules define an extremely large superfamily of nucleic acid binding proteins with several hundred, perhaps thousands of different members in vertebrates. Recent discoveries have pro...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/BF00999848
更新日期:1994-07-01 00:00:00
abstract::Alterations in expression of the DFF40 gene have been reported in some cancers. This study is an in vitro study of the therapeutic effects of gene transfer that lead to elevation in DFF40 expression within T-47D cells in the presence of sulfonamide drugs. In this study, we have constructed a eukaryotic expression vect...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3626-3
更新日期:2014-11-01 00:00:00
abstract::Membrane-bound ribosomes were isolated from a post-mitochondrial supernatant fraction of mouse liver homogenate by sedimentation in a sucrose density gradient, Loose ribosomes were released from the membrane fragments with 0.5 M KCl, while tight bound ribosomes were not released. After purification of the loose and ti...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00778433
更新日期:1980-07-31 00:00:00
abstract::Studies investigating the association between glutathione S-transferase M1 (GSTM1) polymorphism and bladder cancer risk report conflicting results. The objective of this study was to quantitatively summarize the evidence for such a relationship. We performed a systematic search of the National Library of Medline and E...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-010-0386-6
更新日期:2011-04-01 00:00:00
abstract::Natural killer cell (NK) is known as a major immune system in body through mediating cell death via several possible pathways, and one of three subpopulations of lymphocytes functioning as scavenger of tumor, virus infected cells etc. Our present results found that the SOD-contained silkworm larvae powder caused an en...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9165-4
更新日期:2009-01-01 00:00:00
abstract::HSG1 (Heat shock-induced gene 1) is one of the high-temperature-induced proteins in grapevine. Sequence analysis has demonstrated that HSG1 is a grape Bcl-2-associated athanogene (BAG) that contains IQ and BAG domains in its sequence. HSG1 expression was not detected in leaves without heat treatment, while tendrils, s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1224-1
更新日期:2012-04-01 00:00:00
abstract::Hepatitis B virus (HBV) as the main prevalent infectious agent, play important roles in inducing severe liver diseases. Previous studies demonstrated that during prolonged forms of hepatitis B infection including chronic, asymptomatic and occult forms, patients are unable to eradicate HBV from hepatocytes completely. ...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-014-3190-x
更新日期:2014-05-01 00:00:00
abstract::Long non-coding RNAs (LncRNAs) are non-coding RNAs. The potential roles of lncRNAs in type 2 diabetes mellitus (T2DM) are not well-known. In this study, we aim to assess the expression levels of LY86-AS1 and HCG27_201 in T2DM patients and a healthy control group. We obtained whole blood and serum samples from 100 T2DM...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4429-8
更新日期:2018-12-01 00:00:00
abstract::Imprinted genes play important roles in mammalian growth, development and behavior. In this study, we obtained 1568 bp mRNA sequence of porcine DIO3 (deiodinase, iodothyronine, type III), and also identified its imprinting status during porcine fetal development. The complete open reading frame (ORF) encoding 278 amin...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0983-z
更新日期:2012-03-01 00:00:00
abstract::Synthetic hexaploid (SH) wheat derived from crossing tetraploid durum wheat and diploid Aegilops tauschii provide germplasm for wheat improvement as the conventional wheat varieties possess very low genetic diversity. This study aims to identify diverse SH lines which can be used in breeding programs for transferring ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1943-y
更新日期:2013-01-01 00:00:00
abstract::Tumor angiogenesis, a major requirement for tumor growth and metastasis, is regulated by pro- and anti-angiogenic factors. The aim of this study was to quantify the expression of angiogenic (VEGF, HIF-1α, Angiopiotein-2) and anti-angiogenic (endostatin, angiostatin and Thrombospondin-1) factors and to discern their cl...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2690-4
更新日期:2013-10-01 00:00:00
abstract::The purpose of this study was to identify genomic regions, quantitative trait loci (QTL), affecting carcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, two white and wild strains of Japanese quail (16 birds) were crossed reciprocally and F1 generation (34 birds) was created. The F2 ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3316-1
更新日期:2014-07-01 00:00:00
abstract::Seed weight and plant height are important agronomic traits and contribute to seed yield. The objective of this study was to identify QTLs underlying these traits using an intra-specific mapping population of chickpea. A F11 population of 177 recombinant inbred lines derived from a cross between SBD377 (100-seed weigh...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-015-3925-3
更新日期:2015-11-01 00:00:00
abstract::We have cloned two full-length cDNAs from two ferritin genes (Aifer1 and Aifer2) of the bay scallop, Argopecten irradians (Lamarck 1819). The cDNAs are 1,019 and 827 bp in length and encode proteins of 171 and 173 amino acids, respectively. The 5' UTR of each contains a conserved iron response element (IRE) motif. Seq...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0339-0
更新日期:2011-03-01 00:00:00
abstract::Nerve growth factor (NGF) is required for the differentiation and maintenance of sympathetic and sensory neurons. In the present study, the recombinant expression of human nerve growth factor beta (hNGF-β) gene in rabbit bone marrow mesenchymal stem cells (rMSCs) was undertaken. Recombinant vector containing hNGF-β wa...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0068-4
更新日期:2010-12-01 00:00:00
abstract::Substantial evidence has demonstrated that platelet-derived growth factor-D (PDGF-D) is tightly associated with the development and progression of tumors. However, its biological functions in esophageal squamous cell carcinoma (ESCC) remain to be delineated. In this study, we found that expressions of PDGF-D mRNA and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2328-y
更新日期:2013-03-01 00:00:00
abstract::Mitochondrial dysfunction plays a crucial role in the central pathogenesis of insulin resistance and type 2 diabetes mellitus. Macrophages play important roles in the pathogenesis of insulin resistance. Lauric acid is a 12-carbon medium chain fatty acid (MCFA) found abundantly in coconut oil or palm kernel oil and it ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-06019-9
更新日期:2020-12-01 00:00:00
abstract::Berberine (BBR), an effective compound of Chinese traditional herbal medicine, has preventive effects on diabetes and its complications. In this study, we investigated the therapeutic effects and underlying molecular mechanisms of BBR in rats with high-fat diet and streptozotocin (STZ)-induced diabetic nephropathy mod...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2321-5
更新日期:2013-03-01 00:00:00
abstract::Lac insects are commercial scale insects with high economic value. The combined molecular phylogeny of 20 lac insect populations was generated using elongation factor 1 alpha, mitochondrial cytochrome c oxidase subunit I and the small subunit ribosomal RNA gene loci. The 20 populations of lac insects clustered into fo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2701-5
更新日期:2013-10-01 00:00:00
abstract::The Pro12Ala and C161T polymorphisms in peroxisome proliferator-activated receptor γ (PPARγ) have been shown to be associated with carotid artery atherosclerosis. It remains unclear whether these two polymorphisms are associated with risk factors for cardiovascular disease (CVD) in hemodialysis (HD) patients. Therefor...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3645-0
更新日期:2014-11-01 00:00:00
abstract::The Italian National Reference Center for Echinococcosis (CeNRE, Sassari, Italy) set up a diagnostic protocol of "one-step-PCR" useful for the detection of E. granulosus sensu stricto (E.g.s.s.) and the identification of its genotype (G1-G3). The purpose of this work was to perform the validation of the "PCR E.g.s.s."...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04625-w
更新日期:2019-04-01 00:00:00
abstract::Dipeptidyl peptidase III (DPP III) is an emerging biomarker of human cancers. Expression, specificity, and function of human DPP III (hDPP III) mRNA variant I (V-I), II (V-II), and III (V-III) are poorly understood. Here, we investigated expression of these variants in multiple human tumor derived cell lines. DNA sequ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-016-3996-9
更新日期:2016-06-01 00:00:00