Abstract:
:Probiotic bacteria must overcome the toxicity of bile salts secreted in the gut and adhere to the epithelial cells to enable their better colonization with extended transit time. Expression of bile salt hydrolase and other proteins on the surface of probiotic bacteria can help in better survivability and optimal functionality in the gut. Two putative Lactobacillus plantarum isolates i.e., Lp9 and Lp91 along with standard strain CSCC5276 were used. A battery of six housekeeping genes viz. gapB, dnaG, gyrA, ldhD, rpoD and 16S rRNA were evaluated by using geNorm 3.4 excel based application for normalizing the expression of bile salt hydrolase (bsh), mucus-binding protein (mub), mucus adhesion promoting protein (mapA), and elongation factor thermo unstable (EF-Tu) in Lp9 and Lp91. The maximal level of relative bsh gene expression was recorded in Lp91 with 2.89 ± 0.14, 4.57 ± 0.37 and 6.38 ± 0.19 fold increase at 2% bile salt concentration after 1, 2 and 3 h, respectively. Similarly, mub and mapA genes were maximally expressed in Lp9 at the level of 20.07 ± 1.28 and 30.92 ± 1.51 fold, when MRS was supplemented with 0.05% mucin and 1% each of bile and pancreatin (pH 6.5). However, in case of EF-Tu, the maximal expression of 42.84 ± 5.64 fold was recorded in Lp91 in the presence of mucin alone (0.05%). Hence, the expression of bsh, mub, mapA and EF-Tu could be considered as prospective biomarkers for screening of novel probiotic lactobacillus strains for optimal functionality in the gut.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Duary RK,Batish VK,Grover Sdoi
10.1007/s11033-011-1006-9subject
Has Abstractpub_date
2012-03-01 00:00:00pages
2541-52issue
3eissn
0301-4851issn
1573-4978journal_volume
39pub_type
杂志文章abstract::The aim of this study was to identify molecular markers to be applied to marker-assisted selection. Three SNPs of the FASN gene were studied. PCR-RFLP was used for genotyping. The SNPs g.17924A>G, g.17860C>T and g.15603A>G all in the FASN gene were genotyped using the enzymes MscI, DdeI and Hae III, respectively. The ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1883-6
更新日期:2012-12-01 00:00:00
abstract::One of the major limitations when attempting to obtain detailed biochemical, biophysical and immunological characterization of plant DNA mismatch repair proteins is their extremely low abundance in vivo under normal growth conditions. An initial analysis of PMS1 transcript level in various Arabidopsis thaliana tissues...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0203-2
更新日期:2011-02-01 00:00:00
abstract::Molecular detection of Giardia duodenalis by polymerase chain reaction (PCR) is difficult in faecal samples due to inhibitors that contaminate DNA preparations, or due to low cyst concentrations. In order to eliminate inhibitors, improve cyst recovery and molecular detection of G. duodenalis, different types of water,...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05224-5
更新日期:2020-02-01 00:00:00
abstract::It is well known that transcriptionally inactive rRNA genes are correlated with DNA hyper-methylation and histone hypo-methylation and there is clear evidence in humans that DNA and histone modification which alter chromatin structure are related to chromosome fragility. Very little is known about the biological cause...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2112-z
更新日期:2013-01-01 00:00:00
abstract::Cerebral aneurysm affects 2-5% of the population and posterior inferior cerebellar artery (PICA) aneurysms account for 1-3% of all intracranial aneurysms. Oxidative stress is known to contribute to the progression of cerebrovascular disease and it may be increased by inflammation, a key contributor to cerebral aneurys...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4374-6
更新日期:2018-12-01 00:00:00
abstract::Trichoderma asperellum, a filamentous soil fungus, is an effective biocontrol agent against many fungal plant pathogenic species. In the present study, we investigated the biological control properties of the strain T. asperellum T4. T. asperellum fermentation products significantly decreased the ability of Rhizoctoni...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0019-0
更新日期:2010-12-01 00:00:00
abstract::Iron may be implicated in the generation of oxidative stress by the catalyzing the Haber-Weiss or Fenton reaction. On the other hand, oxidative stress has been implicated in the pathogenesis of age-related macular degeneration (AMD) and heme oxygenase-1 (HO-1), encoded by the HMOX1 gene and heme oxygenase-2 (HO-2), en...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0955-3
更新日期:2012-03-01 00:00:00
abstract::Fenpropathrin (FEN) is a pyrethroid insecticide. Mammals can be exposed to these compounds with food and water as non-target organisms. Pyrethroids are classified into two types depending on chemical structure and neurotoxic effects. FEN has features of Type I and Type II pyrethroids. There is data that pyrethroids ap...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05480-w
更新日期:2020-06-01 00:00:00
abstract::The cyclin-dependent kinases (CDKs) are a family of serine/threonine kinases, playing an essential role in regulating cell-cycle progression. In our present work, human CDK5 and a novel CDK5 splicing variant, named as CDK5-SV, were cloned from the cDNA library of human testis. CDK5-SV lacking the exon 7 of CDK5 encode...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9752-7
更新日期:2010-06-01 00:00:00
abstract::The rapid progress made over the last 10 years in the identification of individual autoantigens and in the localization of the epitopes involved, has resulted in a parallel reduction in the complexity of the antigen required for the detection of autoantibodies. The ability to use synthetic peptides as antigens is a re...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/BF00464709
更新日期:1992-06-01 00:00:00
abstract::Serotonin receptor (5-HT) is a biogenic amine acting as a neurotransmitter and neuromodulator that mediates various aspects of reproduction and gametogenesis. The full-length nucleotide sequence of Haliotis discus hannai encodes a protein of 417 amino acids with a predicted molecular mass of 46.54 kDa and isoelectric ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05162-2
更新日期:2020-01-01 00:00:00
abstract::Growing data supported that epigenetic modifications, including altered DNA methylation have a potential role in the pathogenesis of autoimmune thyroid diseases (AITD). In the present study we aimed to investigate the methylation status of ICAM-1 gene promoter in patients with AITD. Forty patients with Graves' disease...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04990-6
更新日期:2019-10-01 00:00:00
abstract::Prkar1b gene encodes regulatory type I, beta subunit (RIβ) of cAMP dependent protein kinase A in mouse. Among the various isoforms of regulatory and catalytic subunits that comprise mammalian PKA, RIβ subunit is considered to be one of the important subunits for neuronal functions. This is involved in multiple forms o...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1108-4
更新日期:2012-04-01 00:00:00
abstract::Melanotransferrin (MTf), a member of the transferrin families, plays an important role in immune response. But the research about MTf in sea cucumber is limited till now. In this study, the Melanotransferrin (Aj-MTf) gene was firstly cloned and characterized from the sea cucumber Apostichoupus japonicus by reverse tra...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3243-1
更新日期:2014-06-01 00:00:00
abstract::Anti-EGFR monoclonal antibodies (anti-EGFR MoAbs) in metastatic colorectal cancer (mCRC) treatment are still not effective in all patients. This study aimed to evaluate the relationship between BRAF V600E mutation and the tumor response of anti-EGFR MoAbs for first-line treatment in mCRC patients. We searched the MEDL...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-013-2974-8
更新日期:2014-03-01 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is a common, complex disorder associated with substantial morbidity and mortality, influenced by both environmental factor and genetic factor. ADAM33 gene was found to be associated with asthma, declined lung function and COPD. The purpose of the study was to test whether S...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0637-6
更新日期:2011-11-01 00:00:00
abstract::Mollusks lack an adaptive immune system and rely solely on the innate immune response. The nuclear factor-kappa B (NF-κB) signaling pathway is one of the most important components of the innate immune system and its activity is regulated by physical interaction with the inhibitor of NF-κB (IκB) protein. The manila cla...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2196-5
更新日期:2013-02-01 00:00:00
abstract::RNA-protein interaction in the 30S subunits of rat liver hnRNP has been studied by crosslinking of informofer proteins to hnRNA induced by UV irradiation. Irradiation of 30S particles with 254 nm UV light in doses of 1 1 x 10(5) erg/mm2 leads to the extensive crosslinking hnRNA to informofer proteins. The crosslinked ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00778513
更新日期:1982-03-31 00:00:00
abstract::The cbb (3)-type oxidases are members of the heme-copper oxidase superfamily, distant by sequence comparisons, but sharing common functional characteristics. The cbb (3) oxidases are missing an active-site tyrosine residue that is absolutely conserved in all A and B-type heme-copper oxidases. This tyrosine is known to...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0437-z
更新日期:2011-06-01 00:00:00
abstract::The objective of this study was to determine hepatic expression levels of GHR, IGF1R, IGF1 and IGF2 genes in young growing gilts at different developmental ages (60-210 days) in five pig breeds: Polish Large White (PLW), Polish Landrace (PL), Pulawska (Pul), Duroc (Dur) and Pietrain (Pie). We studied the differences a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1068-8
更新日期:2012-03-01 00:00:00
abstract::We utilized Percoll density gradient centrifugation to isolate and fractionate chloroplasts of Korean winter wheat cultivar cv. Kumgang (Triticum aestivum L.). The resulting protein fractions were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE) coupled with LTQ-FTICR mass spectrometry. This e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1302-4
更新日期:2012-05-01 00:00:00
abstract::Uterine leiomyomas (UL) are prevalent benign tumors, especially among women of African ancestry. The disease also has genetic liability and is influenced by risk factors such as hormones and obesity. This study investigates the haplotypes of the Cytochrome P450 1B1 gene (CYP1B1) related to hormones and coiled-coil dom...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04790-y
更新日期:2019-06-01 00:00:00
abstract::Se is an essential trace element and is found as a selenocysteine in the active site of Se-enzymes, such as glutathione peroxidase. tRNASec is first aminoacylated with serine by Ser RS and further is converted to selenocysteyl-tRNA by selenocysteine synthase. Mammalian selenocysteine tRNA has dual identities with Ser ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1006879820805
更新日期:1998-11-01 00:00:00
abstract::Myocardial ischemia and reperfusion (MI/R) is associated with an intense inflammatory reaction, which may lead to myocyte injury. In this study, we investigated the effect of quercetin, an inhibitor of c-Jun N-terminal kinase on ischemia/reperfusion injury in isolated rat hearts. Rat models of MI/R were induced by cor...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2002-4
更新日期:2012-12-01 00:00:00
abstract::RACK1 is a crucial scaffold and anchoring protein, which plays a vital role in multiple signaling pathways of tumorigenesis. The aim of the present study was to identify the correlation between expressions of RACK1 and malignant degrees in colorectal carcinoma (CRC) patients. All together 157 CRC patients were enrolle...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3201-y
更新日期:2014-05-01 00:00:00
abstract::In the present study, Amukkara Chooram (AC) a well known herbal medicine was investigated for their antibiofilm efficacy against biofilm of Candida albicans. The biofilm inhibitory concentration of 20 µg/mL of AC showed promising effect by inhibiting the biofilm upto 60%. Morphogenic transition state of C. albicans fr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04757-z
更新日期:2019-06-01 00:00:00
abstract::Ran is a multifunctional small GTPase involved in important cellular activities like nucleocytoplasmic transport, mitotic spindle assembly, nuclear envelope formation, etc., but is also known to be differentially expressed in response to abiotic stress, particularly low temperature. We have over-expressed Lepidium lat...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3476-z
更新日期:2014-09-01 00:00:00
abstract::Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0736-z
更新日期:2012-01-01 00:00:00
abstract::Emergence of MDR 'superbugs' inflamed a severe sense of urgency amongst scientists aiming at the discovery of novel potential drug molecules. Bacteria of the genus Streptomyces are really worth investigating for their immense potential to produce natural compounds of pharmaceutical importance. In the present study, th...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05731-w
更新日期:2020-09-01 00:00:00
abstract::Purified cucumber mosaic virus (CMV) RNA was reacted with NaIO4 and then [3H]KBH4 to label ends containing exposed 2',3'-hydroxyls. The four major RNA species were then fractionated by polyacrylamide gel electrophoresis. Analysis of digests of these RNAs by ribonucleases T1 plus T2, alkaline phosphatease, snake venom ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00357014
更新日期:1975-12-01 00:00:00