Abstract:
:Prkar1b gene encodes regulatory type I, beta subunit (RIβ) of cAMP dependent protein kinase A in mouse. Among the various isoforms of regulatory and catalytic subunits that comprise mammalian PKA, RIβ subunit is considered to be one of the important subunits for neuronal functions. This is involved in multiple forms of synaptic plasticity, and influences memory and learning by maintaining hippocampal long-term potentiation (LTP). Deficient expression of this gene has been implicated in autoimmune disease systemic lupus erythematosus (SLE). We have identified two novel non-coding exons of the Prkar1b gene (designated as exon 1A and exon 1B), which are spliced to the canonical exon 2 and constitute the 5' untranslated region giving rise to three alternative transcript isoforms. We have also confirmed the expression of the previously known first exon (designated as exon 1C) with known transcript published earlier. The transcripts containing exons 1A, 1B and 1C are differentially regulated during the development and tissue types. In silico study of more than 20 kb nucleotide sequence upstream of known translational initiation codon revealed three distinct promoter regions named as PA, PB, and PC upstream of the exon 1A, exon 1B and exon 1C respectively. PB is non-CpG related promoter but PA and PC are CpG related promoters, however all three promoters are TATA less. Further analysis showed that these promoters possess potential signature sequences for common as well as different transcription factors suggesting complex regulation of Prkar1b gene.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Banday AR,Azim S,Tabish Mdoi
10.1007/s11033-011-1108-4subject
Has Abstractpub_date
2012-04-01 00:00:00pages
3375-83issue
4eissn
0301-4851issn
1573-4978journal_volume
39pub_type
杂志文章abstract::HIV/AIDS remains to be one of the killing diseases of mankind. Host genetic response is one of the factor which determine susceptibility to HIV and disease progression to AIDS. The aim of the present study was to evaluate the impact of ERCC2 Lyc ( 751 ) Gln (excision repair cross complementing rodent repair deficiency...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-9958-8
更新日期:2011-06-01 00:00:00
abstract::DNA-, RNA- and protein synthesis have been studied in Mycobacterium smegmatis cells infected with phage I3. The macromolecular synthesis continued until the end of latent period. Early RNA and protein synthesis were necessary prior to the commencement of DNA replication. The infecting phage DNA sedimented as larger th...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00798378
更新日期:1981-11-30 00:00:00
abstract::A cell-free system derived from Dictyostelium discoideum has been used to study the kinetics of inhibition of RNase P by puromycin, amicetin and blasticidin S. Detailed kinetic analysis showed that the type of inhibition of RNase P activity by puromycin is simple competitive, whereas the type of inhibition by amicetin...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1022290110116
更新日期:2003-03-01 00:00:00
abstract::Epidemiological studies found inconsistent results on the association of two variants on TGFBR1 (TGFBR1*6A and Int7G24A) with colorectal cancer (CRC) risk. The present study was aimed to evaluate the association of these two variants with CRC susceptibility via the meta-analysis methods. For variant TGFBR1*6A, nine re...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-1009-6
更新日期:2012-03-01 00:00:00
abstract::In this study, we cloned and sequenced genomic sequences from a Fenneropenaeus chinensis transcriptional repressor gene, FcTR. The FcTR gene is 2,671 bp in length and has four exons and three introns. The 873 bp promoter contains several transcription factor binding sites, including a TATA box and a cyclic AMP-respons...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3780-7
更新日期:2015-02-01 00:00:00
abstract::A method is described which demonstrates the posibility of visualizing protein-complexed DNA by using pronase for both deproteinization and spreading of DNA for electron microscopy. The results show that proteolytic digestion is complete even under conditions of short formaldehyde fixation and pronase is an excellent ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00778415
更新日期:1979-08-31 00:00:00
abstract::Bud dormancy in perennial plants adapts to environmental and seasonal changes. Bud dormancy is of ecological interest because it affects forest population growth characteristics and is of economical interest because it impacts wood production levels. To understand Pinus sylvestris L. var. mongolica litv. bud-dormancy ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0159-2
更新日期:2011-02-01 00:00:00
abstract::Molecular abnormalities in the 11p15.5 imprinted gene cluster lead to two different growth diseases: Beckwith-Wiedemann syndrome (BWS) and Silver-Russell syndrome (SRS). They are mainly caused by epigenetic alterations in one of the two imprinting 11p15 control regions (ICR1 and ICR2). These CpG-rich regions are diffe...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2057-2
更新日期:2013-01-01 00:00:00
abstract::The interaction between benzophenone (BP) and bovine serum albumin (BSA) was investigated by the methods of fluorescence spectroscopy combined with UV-Vis absorption and circular dichroism (CD) measurements under simulative physiological conditions. The experiment results showed that the fluorescence quenching of BSA ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0380-z
更新日期:2011-04-01 00:00:00
abstract::The 1,044 bp endo-1,4-β-xylanase gene of a hyperthermophilic Eubacterium, "Thermotoga petrophila RKU 1" (T. petrophila) was amplified, from the genomic DNA of donor bacterium, cloned and expressed in mesophilic host E. coli strain BL21 Codon plus. The extracellular target protein was purified by heat treatment followe...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1555-6
更新日期:2012-07-01 00:00:00
abstract::The oriental melon (Cucumis melo var. makuwa), called 'chamoe' in Korean, is a popular fruit crop cultivated mainly in Asia and a high-market value crop in Korea. To provide molecular breeding resources for chamoe, we developed and characterized genomic SSR markers from the preliminary Illumina read assemblies of Gotg...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2803-0
更新日期:2013-12-01 00:00:00
abstract::Microsatellites, or simple sequence repeats (SSRs), are very useful molecular markers for a number of plant species. We used a new publicly available module (TROLL) to extract microsatellites from the public database of soybean expressed sequence tag (EST) sequences. A total of 12,833 sequences containing di- to penta...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1776-8
更新日期:2012-09-01 00:00:00
abstract::Published data on the association of vascular endothelial growth factor (VEGF) -1154G>A polymorphism with cancer risk is inconclusive. To derive a more precise estimation of association between VEGF -1154G>A polymorphism and the risk of cancer, we performed a meta-analysis of 7,071 cancer cases and 7,693 controls from...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-1326-9
更新日期:2012-05-01 00:00:00
abstract::Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper alternative to more commonly used methods of producing transgenic animals. In this study, the capra semen ejaculates were pooled together and then incubated in vitro with DIG-labeled DNA. The binding and internalizing rates were observe...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1019-4
更新日期:2012-03-01 00:00:00
abstract::The two predominant forms of arginase, cytosolic Arginase-I and mitochondrial Arginase-II, catalyze hydrolysis of arginine into ornithine and urea. Based on presence of arginase activity in extracts using potassium chloride (KCl), mitochondrial membrane-bound arginase has also been suggested. However, the activity of ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-016-3965-3
更新日期:2016-05-01 00:00:00
abstract::The complete mitochondrial genome of Zhikong scallop Chlamys farreri is 21,695 bp in length and contains 12 protein-coding genes (the atp8 gene is absent, as in most bivalves), 2 ribosomal RNA genes, and 22 transfer RNA genes. The heavy strand has an overall A+T content of 58.7%. GC and AT skews for the mt genome of C...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-9974-8
更新日期:2011-06-01 00:00:00
abstract::Cardiomyocytes possess the ability to respond to mechanical stimuli by reprogramming their gene expression. This study investigated the effects of different loading protocols on signaling and expression responses of myogenic, anabolic, inflammatory, atrophy and pro-apoptotic genes in cardiomyocyte-like H9C2 cells. Dif...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-06112-z
更新日期:2021-01-04 00:00:00
abstract::Studies investigating the association between glutathione S-transferase M1 (GSTM1) polymorphism and bladder cancer risk report conflicting results. The objective of this study was to quantitatively summarize the evidence for such a relationship. We performed a systematic search of the National Library of Medline and E...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-010-0386-6
更新日期:2011-04-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) has been investigated extensively at the molecular level. Nevertheless, genome wide study on the temporal and spatial dynamics of the virus is non-existed. To explore this topic, we determined complete coding genome sequences for four PRRSV isolates and analy...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2802-1
更新日期:2013-10-12 00:00:00
abstract::Bacterial artificial chromosome (BAC) library is an important genomic resource useful in targeted marker development, positional cloning, physical mapping and a substrate for genome sequencing for better understanding the genome organization of a species. The present manuscript elucidates the improvement in protocols ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05283-z
更新日期:2020-03-01 00:00:00
abstract::Familial Mediterranean fever is an autosomal recessive disorder characterized by recurrent attacks of abdominal pain, synovitis and pleuritis. MEFV gene mutations are responsible for the disease. The objective of this study was to identify the frequency and distribution of 12 MEFV mutations in 153 Syrian patients and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9475-9
更新日期:2010-01-01 00:00:00
abstract::Patients with β-thalassemia suffer from a lack or absence of the beta-globin chain of normal hemoglobin (Hb). Therefore, an increase in fetal Hb (HbF) levels could improve the clinical status of these patients. Downregulation of BCL11A, a key regulatory transcription factor, could ameliorate the clinical status of tha...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05483-7
更新日期:2020-05-01 00:00:00
abstract::Methyltransferases play essential roles in modulating important cellular and metabolic processes. A mouse putative N6-DNA methyltransferase gene (GenBank No AY456393) is a novel gene named mN6amt1(mN6A1). To investigate its function in cell fate and protein translation, RNA interference (RNAi)-mediated knock-down meth...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9243-2
更新日期:2009-04-01 00:00:00
abstract::Membrane-bound Δ9 desaturase perform oxygenated desaturation reactions to insert the first double bonds within fatty acyl chains between C9 and C10 positions of most saturated substrates. Arthrospira platensis, a blue green microalga, is an important source of polyunsaturated fatty acids (PUFA) such as oleic, linoleic...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4333-2
更新日期:2018-12-01 00:00:00
abstract::Human Papillomavirus infection is considered as the main etiological factor of cervical cancer (ICC), although, the role of host genetic factors in ICC susceptibility has been increasing. Immunological response is crucial for the prevention of viral associated diseases. Interleukin 1 receptor antagonist (IL-1RN) is co...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1979-z
更新日期:2012-12-01 00:00:00
abstract::Deprivation of cytokines induces cell cycle arrest and apoptosis in cytokine-dependent hematopoietic progenitors. Previous studies have indicated that in Baf-3, interleukin (IL)-3-dependent cells, apoptosis is caused predominantly by Bim, a BH3-only cell death activator that belongs to the Bcl-2 superfamily. Because B...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-004-7656-0
更新日期:2005-06-01 00:00:00
abstract::Salvia lachnostachys is an herbaceous plant with anti-inflammatory, analgesic and cytotoxic properties. This study investigated the antitumor effect of an ethanolic extract of Salvia lachnostachys leaves (EES) in a solid Ehrlich carcinoma model. Ehrlich cells were inoculated subcutaneously in the right pelvic member (...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04931-3
更新日期:2019-10-01 00:00:00
abstract::Apolipoprotein AV (apoAV) modulates plasma triglyceride levels, which is an independent risk factor for cardiovascular disease. ApoAV is also involved in atherosclerosis lesion formation. In order to systematically evaluate the apolipoprotein-related gene profile in tree shrew, a model for its insusceptibility to athe...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2641-0
更新日期:2013-09-01 00:00:00
abstract::Heparan sulfate proteoglycans (HSPGs) located at the cell surface and in the extracellular matrix of most animal tissues are proteoglycan coreceptors that carry heparan sulfate chains, and play a vital role in infections of many diseases. HSPGs are classified as glypican, syndecan, perlecan and agrin according to diff...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2340-2
更新日期:2013-03-01 00:00:00
abstract::Recent evidence supports a role of Toll-like receptor (TLR) signaling in the development of atherosclerotic lesions. It was confirmed that the presence of functional TLR4 promotes a proinflammatory phenotype and proliferation of vascular smooth muscle cells (VSMCs). Here we tested whether designed TLR4 small interferi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0246-4
更新日期:2011-02-01 00:00:00