Improved protocols for BAC insert DNA isolation, BAC end sequencing and FISH for construction of BAC based physical map of genes on the chromosomes.

abstract：:Master differentiation transcription factors (MDFs) play decisive roles in cell lineage commitment and cellular functions. Forkhead box P3 (Foxp3) is the MDF essential for the lineage commitment and the suppression function of regulatory T cells (Tregs), which play critical roles in suppression of autoimmunity. Here, ...

journal_title：Molecular biology reports

authors： Fujita T,Fujii H

更新日期：2012-10-01 00:00:00

abstract：:Lung cancer is the most common cause of cancer death worldwide. Smoking is known as the strongest single factor in the development of lung cancer. However, there are inherited genetic factors that cause different responses to cigarette smoking exposure among individuals. We tried to identify these differences in heavy...

journal_title：Molecular biology reports

authors： Lee M,Lee Y,Cho HJ,Hong J,Kwon SJ,Park CG,Lee HY,Son JW,Kang J

更新日期：2011-11-01 00:00:00

abstract：:In spite of much awareness, diabetes mellitus continues to remain one of major reasons for mortality and morbidity rate all over the globe. Free radicals cause oxidative stress which is responsible for causing diabetes. The recent advancements in elucidation of ARE/keap1/Nrf2 pathway can help in better understanding o...

journal_title：Molecular biology reports

authors： Behl T,Kaur I,Sehgal A,Sharma E,Kumar A,Grover M,Bungau S

更新日期：2021-01-03 00:00:00

abstract：:Pathologic complete response after neoadjuvant systemic treatment appears to be a valid surrogate for better overall survival in breast cancer patients. Currently, together with standard clinicopathologic assessment, novel molecular biomarkers are being exhaustively tested in order to look into the heterogeneity of br...

journal_title：Molecular biology reports

authors： Kolacinska A,Fendler W,Szemraj J,Szymanska B,Borowska-Garganisz E,Nowik M,Chalubinska J,Kubiak R,Pawlowska Z,Blasinska-Morawiec M,Potemski P,Jeziorski A,Morawiec Z

更新日期：2012-07-01 00:00:00

abstract：:Growth hormone-releasing hormone receptor (GHRHR) has important functions in the regulation of the growth hormone axis and the development and proliferation of pituitary somatotropes. Moreover, some mutations in mouse GHRHR can induce the dwarfism. The objective of this paper is to reveal the association of GHRHR with...

journal_title：Molecular biology reports

authors： Zhang CF,Chen H,Zhang ZY,Zhang LZ,Yang DY,Qu YJ,Hua LS,Zhang B,Hu SR

更新日期：2012-12-01 00:00:00

abstract：:'Molecular sandwiches' composed of two aromatic amino acids separated by a hydrophilic one were found on eleven subspecies of human interferon alpha, on murine interferon alpha 2, and human interferon beta 1. In addition, another type of the sandwiches was found on several species of interferon. This confirms and exte...

journal_title：Molecular biology reports

authors： Inglot AD,Popik W,Piasecki E,Czyrski J

更新日期：1986-01-01 00:00:00

abstract：:A member of satellite repetitive DNA was isolated and sequenced from a saltwater fish Sillago japonica (Percoidei). This sequence consists of several oligo-dA/dT tracts and two inverted repeats which resemble each other. Dot blot hybridization analysis using a satellite DNA clone pSJ2 among the species in the suborder...

journal_title：Molecular biology reports

authors： Kato M,Yoshida M

更新日期：1995-01-01 00:00:00

abstract：:Phage display procedure was applied to the N-terminal domain of human topoisomerase I. The consensus sequence identified for clones binding to the N-terminal domain was found in 35 human proteins that are either permanently or temporarily located in the nucleus. They are in majority involved in the DNA repair, transcr...

journal_title：Molecular biology reports

authors： Trzcińska AM,Girstun A,Piekiełko A,Kowalska-Loth B,Staroń K

更新日期：2002-12-01 00:00:00

abstract：:Small ubiquitin-like modifier (SUMO) participates in post-translational modification of various target proteins. SUMOylation is an important molecular regulatory mechanism for plants to respond to abiotic stress. In the present study, GmSUMO2 gene was isolated from soybean seedlings for further study because of the hi...

journal_title：Molecular biology reports

authors： Guo J,Wang S,Wang G,Lu R,Wang Y,Guo Y,Ji W

更新日期：2020-05-01 00:00:00

abstract：:Membrane-bound ribosomes were isolated from a post-mitochondrial supernatant fraction of mouse liver homogenate by sedimentation in a sucrose density gradient, Loose ribosomes were released from the membrane fragments with 0.5 M KCl, while tight bound ribosomes were not released. After purification of the loose and ti...

journal_title：Molecular biology reports

authors： Hoffman WL,Dowben RM

更新日期：1980-07-31 00:00:00

abstract：:Hepatocellular carcinoma (HCC) incidence is fast-growing especially in countries highly prevalent with viral hepatitis. Its poor prognosis has driven the research toward the discovery of sensitive markers for early detection. We investigated the usefulness of serum Transforming growth factor-beta1 (TGF-β1), Glypican-3...

journal_title：Molecular biology reports

authors： Ibrahim GH,Mahmoud MA,Aly NM

更新日期：2013-12-01 00:00:00

abstract：:Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper alternative to more commonly used methods of producing transgenic animals. In this study, the capra semen ejaculates were pooled together and then incubated in vitro with DIG-labeled DNA. The binding and internalizing rates were observe...

journal_title：Molecular biology reports

authors： Zhao Y,Yu M,Wang L,Li Y,Fan J,Yang Q,Jin Y

更新日期：2012-03-01 00:00:00

abstract：:Current study was conducted to investigation of effect of magnetic field on cell dedifferentiation and follow it callus induction derived from mature embryo culture in bread wheat genotypes. For this purpose, a factorial experiment based on completely randomized design was carried out with two wheat genotypes and thre...

journal_title：Molecular biology reports

authors： Kahrizi D,Cheghamirza K,Akbari L,Rostami-Ahmadvandi H

更新日期：2013-02-01 00:00:00

abstract：:The tertiary structure of nucleic acids results from an equilibrium between electrostatic interactions of phosphates, stacking interactions of bases, hydrogen bonds between polar atoms and water molecules. Water interactions with ribonucleic acid play a key role in its structure formation, stabilization and dynamics. ...

journal_title：Molecular biology reports

authors： Giel-Pietraszuk M,Barciszewski J

更新日期：2012-05-01 00:00:00

abstract：:The purpose of this study was to assess the value of cytokeratin 19 (CK19) and matrix metalloproteinase 2 (MMP-2) in predicting lymph node metastasis (LNM) and survival after curative resection in hepatocellular carcinoma (HCC) patients. Expression of CK19 and MMP-2 in tumor tissue was assessed through immunohistochem...

journal_title：Molecular biology reports

authors： Xiang ZL,Zeng ZC,Tang ZY,Fan J,Sun HC,Tan YS

更新日期：2011-06-01 00:00:00

abstract：:The multidrug and toxic compound extrusion (MATE) protein family is a newly discovered family of secondary transporters that extrude metabolic waste and a variety of antibiotics out of the cell using an electrochemical gradient of H+ or Na+ across the membrane. The main function of MATE gene family is to participate i...

journal_title：Molecular biology reports

authors： Li Y,He H,He LF

更新日期：2019-02-01 00:00:00

abstract：:Human type II inosine monophosphate dehydrogenase (IMPDH2) is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. Several Single Nucleotide Polymorphisms (SNP) affecting the IMPDH2 gene sequence have been reported with potent...

journal_title：Molecular biology reports

authors： Garat A,Cardenas CL,Lionet A,Devos A,Glowacki F,Kenani A,Migot-Nabias F,Allorge D,Lo-Guidice JM,Broly F,Cauffiez C

更新日期：2011-11-01 00:00:00

abstract：:Evidence indicates that both S-adenosylmethionine (SAMe) metabolism and intramuscular fat are associated with insulin resistance and type II diabetes. However, it is still unknown whether SAMe have effects on intramuscular adipogenesis. The present study investigated the roles of SAMe in the adipogenic differentiation...

journal_title：Molecular biology reports

authors： Yue T,Fang Q,Yin J,Li D,Li W

更新日期：2010-10-01 00:00:00

abstract：:A full-length cDNA encoding wheat peroxisomal ascorbate peroxidase (pAPX) was cloned by Suppression Subtractive Hybridization (SSH) and in silico approach. The cDNA was 1027 bp in length and contained a complete ORF of 876 bp, which encodes a protein of 292 amino acid residues. Its deduced amino acids sequence had 84%...

journal_title：Molecular biology reports

authors： Chen Y,Wang H,Wang X,Cao A,Chen P

更新日期：2006-09-01 00:00:00

abstract：:Wild Solanum species are the important resources for potato improvement. With the availability of potato genome and sequencing progress, knowledge about genomic resources is essential for novel genes discovery. Hence, the aim of this study was to decipher draft genome sequences of unique potato genotypes i.e. somatic ...

journal_title：Molecular biology reports

authors： Tiwari JK,Rawat S,Luthra SK,Zinta R,Sahu S,Varshney S,Kumar V,Dalamu D,Mandadi N,Kumar M,Chakrabarti SK,Rao AR,Rai A

更新日期：2021-01-13 00:00:00

abstract：:The Great-billed Seed-finch, Sporophila maximiliani, is a threatened neotropical bird that has declined mainly due to illegal trapping, with very few records in the wild in the last two decades. Despite the existence of a considerable captive population that could be used for reintroductions into the wild, many indivi...

journal_title：Molecular biology reports

authors： Medolago CAB,Costa MC,Ubaid FK,Glenn TC,Silveira LF,Francisco MR

更新日期：2018-12-01 00:00:00

abstract：:In our research, signal transducer and activator of transcription 5b (STAT5b) gene was studied as candidate gene associated with body weight and reproductive traits of Jinghai Yellow chicken. Single nucleotide polymorphisms (SNPs) of STAT5b gene were examined in both Jinghai Yellow chicken and three reference chicken ...

journal_title：Molecular biology reports

authors： Zhao XH,Wang JY,Zhang GX,Wei Y,Gu YP,Yu YB

更新日期：2012-04-01 00:00:00

abstract：:Generation of induced pluripotent stem cells from endangered cattle breeds makes a significant contribution to the establishment of embryonic stem cell line, conservation and utilization of genetic resources in endangered cattle. In our study, we are trying to construct recombinant proteins of transcription factors Oc...

journal_title：Molecular biology reports

authors： Hu PF,Guan WJ,Li XC,Ma YH

更新日期：2012-06-01 00:00:00

abstract：:We studied the distribution of the D/D, I/D, and I/I genotypes of the angiotensin-converting enzyme (ACE) in a sample of healthy Lebanese individuals to assess their prevalence and compare them with other populations. ACE genotypes were determined using the Cardiovascular Disease (CVD) StripAssay, which is based on a ...

journal_title：Molecular biology reports

authors： Sabbagh AS,Otrock ZK,Mahfoud ZR,Zaatari GS,Mahfouz RA

更新日期：2007-03-01 00:00:00

abstract：:The Cdx-2 polymorphism in VDR gene has been extensively investigated for association with cancer risk, however, results of different studies have been inconsistent. The objective of this study is to assess the relationship of the Cdx-2 polymorphism in VDR and cancer risk by meta-analysis. All eligible case-control stu...

journal_title：Molecular biology reports

authors： Huang J,Huang J,Ma Y,Wang H,Yang J,Xiong T,Du L

更新日期：2013-07-01 00:00:00

abstract：:The interaction between benzophenone (BP) and bovine serum albumin (BSA) was investigated by the methods of fluorescence spectroscopy combined with UV-Vis absorption and circular dichroism (CD) measurements under simulative physiological conditions. The experiment results showed that the fluorescence quenching of BSA ...

journal_title：Molecular biology reports

authors： Zhang YZ,Zhang J,Li FF,Xiang X,Ren AQ,Liu Y

更新日期：2011-04-01 00:00:00

abstract：:The objective of this study was to determine hepatic expression levels of GHR, IGF1R, IGF1 and IGF2 genes in young growing gilts at different developmental ages (60-210 days) in five pig breeds: Polish Large White (PLW), Polish Landrace (PL), Pulawska (Pul), Duroc (Dur) and Pietrain (Pie). We studied the differences a...

journal_title：Molecular biology reports

authors： Pierzchała M,Pareek CS,Urbański P,Goluch D,Kamyczek M,Różycki M,Smoczynski R,Horbańczuk JO,Kurył J

更新日期：2012-03-01 00:00:00

abstract：:Probiotic bacteria must overcome the toxicity of bile salts secreted in the gut and adhere to the epithelial cells to enable their better colonization with extended transit time. Expression of bile salt hydrolase and other proteins on the surface of probiotic bacteria can help in better survivability and optimal funct...

journal_title：Molecular biology reports

authors： Duary RK,Batish VK,Grover S

更新日期：2012-03-01 00:00:00

abstract：:The association between vascular endothelial growth factor (VEGF) gene polymorphisms and risk of head and neck cancer (HNC) were investigated in many published studies; however, the currently available results are inconclusive. Therefore, we conducted this meta-analysis for deriving a more precise estimation of associ...

journal_title：Molecular biology reports

authors： Leng WD,He MN,Chen QL,Gong H,Zhang L,Zeng XT

更新日期：2013-10-01 00:00:00

abstract：:As a kind of binding protein, the type 1 Na(+)/H(+) exchanger (NHE1) is a receptor for the highly pathogenic Avian leukosis viruses-J subgroup (ALV-J) in chicken. In order to investigate the potential effect of chicken NHE1 gene on leukosis, we compared its expression between ALV-J-affected and -unaffected chicken, sc...

journal_title：Molecular biology reports

authors： Chen B,Pan W,Zhang L,Liu J,Ouyang H,Nie Q,Zhang X

更新日期：2014-10-01 00:00:00