Abstract：
:Evidence indicates that both S-adenosylmethionine (SAMe) metabolism and intramuscular fat are associated with insulin resistance and type II diabetes. However, it is still unknown whether SAMe have effects on intramuscular adipogenesis. The present study investigated the roles of SAMe in the adipogenic differentiation of porcine muscle satellite cells. Cells isolated from neonatal pig muscle were treated with different concentrations of SAMe (0, 0.5 and 1.0 mM) for 24 h, induced for a 9-day adipogenic differentiation and were finally stained by oil red O staining. The adipocyte determination and differentiation factor-1 (ADD1) and peroxisome proliferator-activated receptor gamma (PPARγ) mRNA and protein were stimulated by SAMe treatment in a dose-dependent manner. Lipoprotein lipase (LPL) mRNA and protein were enhanced in 1.0 mM treatment group, compared with the control. No significant difference was observed in the intracellular lipid content among treatments. These results provide evidence that SAMe may be associated with intramuscular adipogenesis and indicate a novel action of SAMe in fat metabolism.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Yue T,Fang Q,Yin J,Li D,Li Wdoi
10.1007/s11033-009-9893-8subject
Has Abstractpub_date
2010-10-01 00:00:00pages
3143-9issue
7eissn
0301-4851issn
1573-4978journal_volume
37pub_type
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