Abstract:
:With an aim at detecting the ultra-low concentration of avian influenza virus (AIV), a highly sensitive hybrid assay based on immunology and polymerase chain reaction was developed. The TopYield microtiter plates were coated with ten-fold serial dilutions of H5N1 subtype AIV ranging from 10 EID(50 )ml(-1)~10(-4) EID(50) ml(-1),which was recognized by mouse anti-AIV H5 monoclonal antibody (MAb) that was directly linked with reporter DNA using a heterobifunctional cross-linker. After extensive washing, the reporter DNA including a BamH I-restriction site was released by a specific enzymatic restriction, then transferred to PCR tubes, amplified, and used as the signal for detection of AIV. Under the optimized condition, MAb-based immuno-PCR (IPCR) method could measure 100 µl of AIV H5N1 with 10(-4 )EID(50) ml(-1).To evaluate the sensitivity of IPCR, the same concentration and volume of AIV H5N1 were detected by conventional RT-PCR and sandwich ELISA. The results showed that IPCR had an approximately 1,000-fold improvement over the conventional ELISA, and a 100-fold enhancement compared with RT-PCR in detection sensitivity. To further evaluate the specificity of IPCR for AIV H5 subtype, the tracheal swab specimens, taken from chickens which were infected with H9N2, and the allantoic fluid from eggs inoculated by AIV H3N2, H7N1, H9N2, were detected by IPCR. To mimic clinical samples, pharyngeal-tracheal swab specimens were collected from healthy chickens and spiked with H5N1, H5N2, H5N3 for analysis by immuno-PCR. The results demonstrated that IPCR was a highly sensitive and specific assay for AIV H5, and could be applied to clinical detection for low amount of AIV H5 subtype. This MAb-based immuno-PCR method provided a platform capable of mass screening of clinical samples for AIV H5 subtype and could serve as a model for other immuno-PCR assays.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Deng MJ,Xiao XZ,Zhang YM,Wu XH,Zhu LH,Xin XQ,Wu DLdoi
10.1007/s11033-010-0315-8subject
Has Abstractpub_date
2011-03-01 00:00:00pages
1941-8issue
3eissn
0301-4851issn
1573-4978journal_volume
38pub_type
杂志文章abstract::Common bean is gaining acceptance as one of the most valuable major food consumed worldwide owing to innumerable nutritional and therapeutic benefits. Comparatively less productivity in underdeveloped countries encouraged us to proceed for QTL mining of yield traits in common bean. Heretofore, multiple yield associate...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05735-6
更新日期:2020-09-01 00:00:00
abstract::Substantial epidemiological studies have shown an association of obesity with the common gynecological malignancy, endometrial cancer. The relevant interactions and contribution of estradiol and the adipose cytokine, leptin, in endometrial lesions are not completely understood. Suitable animal models to understand the...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05116-8
更新日期:2020-01-01 00:00:00
abstract::Macrophage activation is modulated by both environmental cues and endogenous programs. In the present study, we investigated the role of a PAQR family protein, monocyte to macrophage differentiation-associated (MMD), in macrophage activation and unveiled its underlying molecular mechanism. Our results showed that whil...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1370-5
更新日期:2012-05-01 00:00:00
abstract::In this study, we cloned and sequenced genomic sequences from a Fenneropenaeus chinensis transcriptional repressor gene, FcTR. The FcTR gene is 2,671 bp in length and has four exons and three introns. The 873 bp promoter contains several transcription factor binding sites, including a TATA box and a cyclic AMP-respons...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3780-7
更新日期:2015-02-01 00:00:00
abstract::Phage display procedure was applied to the N-terminal domain of human topoisomerase I. The consensus sequence identified for clones binding to the N-terminal domain was found in 35 human proteins that are either permanently or temporarily located in the nucleus. They are in majority involved in the DNA repair, transcr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1021237021338
更新日期:2002-12-01 00:00:00
abstract::Induction and break of bud dormancy are important features for perennial plants surviving extreme seasonal variations in climate. However, the molecular mechanism of the dormancy regulation, still remain poorly understood. To better understand the molecular basis of poplar bud dormancy, we used a label-free quantitati...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2548-9
更新日期:2013-07-01 00:00:00
abstract::Overexpression of ATP-binding cassette (ABC) transporters causing multidrug resistance (MDR) in cancer cells is one of the major obstacles in cancer chemotherapy. The 5-FU resistant subclone (HL-60/5FU) of the human HL-60 promyelocytic leukemia cell line was selected by the conventional method of continuous exposure o...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05017-w
更新日期:2019-12-01 00:00:00
abstract::Intramembrane proteases control many important processes in a wide variety of organisms through regulated intramembrane proteolysis (RIP). However, very few intramembrane proteases have been characterized in plants. Intriguingly, EGY2 in Arabidopsis belongs to the Site-2 protease (S2P) family that performs RIP. It con...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0962-4
更新日期:2012-03-01 00:00:00
abstract::Random amplified polymorphic DNA (RAPD) and microsatellite markers were applied to evaluate the genetic variation in endemic and endangered yellow catfish, Horabagrus brachysoma sampled from three geographic locations of Western Ghat, South India river systems. In RAPD, of 32 10-mer RAPD primers screened initially, 10...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9381-6
更新日期:2009-09-01 00:00:00
abstract::Most living organisms exhibit circadian rhythms in physiology and behavior. These oscillations are generated by an endogenous circadian clock and control many biological processes. Ceramide has attracted attention as a signal mediator in diverse cell processes including cell death and differentiation. The relationship...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1207-2
更新日期:2012-04-01 00:00:00
abstract::C2H2 proteins belong to a group of transcription factors (TFs) existing as a superfamily that plays important roles in defense responses and various other physiological processes in plants. The present study aimed to screen for and identify C2H2 proteins associated with defense responses to abiotic and biotic stresses...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1542-y
更新日期:2012-06-01 00:00:00
abstract::The blue swimming crab (Portunus pelagicus) is a valuable marine fishery resource in Indo-West Pacific Ocean. So far, rare genetic resource of this species is available. In this report, the restriction-site associated DNA (RAD) approach was employed to mine the genomic information and identify molecular markers in P. ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4339-9
更新日期:2018-12-01 00:00:00
abstract::Maize is one of the world's most important crops and a model for grass genome research. Long terminal repeat (LTR) retrotransposons comprise most of the maize genome; their ability to produce new copies makes them efficient high-throughput genetic markers. Inter-retrotransposon-amplified polymorphisms (IRAPs) were use...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05246-4
更新日期:2020-03-01 00:00:00
abstract::The aim of this study was to explore whether the cytotoxic T lymphocyte antigen-4 (CTLA-4) +49 A/G polymorphism confers susceptibility to rheumatoid arthritis (RA). A meta-analysis was conducted on the associations between CTLA-4 +49 A/G polymorphism and RA using; 1) allele contrast, 2) the recessive model, 3) the dom...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-1364-3
更新日期:2012-05-01 00:00:00
abstract::WRKY transcription factor proteins play significant roles in plant stress responses. Here, we report the cloning and characterization of a novel WRKY gene, MusaWRKY71 isolated from an edible banana cultivar Musa spp. Karibale Monthan (ABB group). MusaWRKY71, initially identified using in silico approaches from an abio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0521-4
更新日期:2011-08-01 00:00:00
abstract::Relying on the regulation of transcription factors, plants resist to various abiotic and biotic stresses. NAC (NAM, ATAF1/2, CUC2) are one of the largest families of plant-specific transcription factors and known to play important roles in plant development and response to environmental stresses. A new NAC gene was cl...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2760-7
更新日期:2013-11-01 00:00:00
abstract::We reported earlier about the detection of a chromosomally located arsenic operon (arsRBC) in a gram-negative bacterium Pseudomonas fluorescens strain MSP3, which showed resistance to elevated levels of sodium arsenate and sodium arsenite. The genes for arsenic resistance were cloned into the HindIII site of pBluescri...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1017950207981
更新日期:2001-01-01 00:00:00
abstract::Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional requirements during periods of reproductive activity. Therefore, a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0666-1
更新日期:2011-11-01 00:00:00
abstract::DNA-, RNA- and protein synthesis have been studied in Mycobacterium smegmatis cells infected with phage I3. The macromolecular synthesis continued until the end of latent period. Early RNA and protein synthesis were necessary prior to the commencement of DNA replication. The infecting phage DNA sedimented as larger th...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00798378
更新日期:1981-11-30 00:00:00
abstract::Matrix metalloproteinases (MMPs) are implicated in atherosclerosis evolution into a coronary artery disease (CAD). They could be used as biomarkers for a predictive approach when they are studied simultaneously. We aim in our study to demonstrate prospectively in patients with history of CAD that MMPs level is linked ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04914-4
更新日期:2019-10-01 00:00:00
abstract::Combined treatment with niacin and chromium has been found to have a protective effect against oxidative damage to different tissues of hyperlipidemic rats. But its effects on vascular endothelial dysfunction are less clear. This study was performed to investigate the effect of combined treatment with niacin and chrom...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9309-1
更新日期:2009-07-01 00:00:00
abstract::The present study demonstrates that protein biosynthesis can be studied in single isolated human scalp hair follicles. The matrix and the sheath are the main regions where amino acids are built in. Incorporation is linear for at least five hours. The newly synthesized proteins can be separated into a water-soluble, a ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00777556
更新日期:1979-02-15 00:00:00
abstract::Interleukin-8 (IL8) polymorphisms have been implicated in several cancers, but their roles in the pathogenesis of hepatocellular carcinoma (HCC) are largely unknown. The present study was designed to explore the association between IL8 polymorphism and the risk of HCC in a Chinese population. Four single nucleotide po...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2993-5
更新日期:2014-03-01 00:00:00
abstract::Cell free protein synthesis has become a powerful method for the high-throughput production of proteins that are difficult to express in living cells. The protein SAP2 of Fasciola hepatica (FhSAP2), which has demonstrated to be both, an excellent vaccine candidate against experimental fascioliasis and a good antigen f...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4251-3
更新日期:2018-10-01 00:00:00
abstract::Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were ob...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-016-4077-9
更新日期:2016-12-01 00:00:00
abstract::Rheumatic heart disease (RHD) or acute rheumatic fever (ARF) develops as a consequence of an exaggerated immune response to Group A beta haemolytic streptococci causing pharyngitis. The molecular mimicry appears between human cardiac myosin and M protein of group A streptococcal membranes. The polymorphism of the prot...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0692-7
更新日期:2011-11-01 00:00:00
abstract::The aim of this work was to sequence the promoter region of β-lactoglobulin (BLG) gene in four sheep breeds, in order to identify polymorphisms, infer and analyze haplotypes, and phylogenetic relationship among the Valle del Belice breed and the other three breeds considered as ancestors. Sequencing analysis and align...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0794-2
更新日期:2012-01-01 00:00:00
abstract::Emergence of MDR 'superbugs' inflamed a severe sense of urgency amongst scientists aiming at the discovery of novel potential drug molecules. Bacteria of the genus Streptomyces are really worth investigating for their immense potential to produce natural compounds of pharmaceutical importance. In the present study, th...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05731-w
更新日期:2020-09-01 00:00:00
abstract::Brassica oleracea var. capitata (cabbage) is an economically important crop affected by black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). MicroRNAs (miRNAs) play an important role in plant defense modulation and therefore the analysis of these molecules can help better understand plant-pathogen ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04779-7
更新日期:2019-06-01 00:00:00
abstract::The Rv3203 (LipV) of Mycobacterium tuberculosis (Mtb) H37Rv, is annotated as a member of Lip family based on the presence of characteristic consensus esterase motif ‘GXSXG’. In vitro culture studies of Mtb H37Ra indicated that expression of Rv3203 gene was up-regulated during acidic stress as compared to normal wherea...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2861-3
更新日期:2014-01-01 00:00:00