Quantitative expression of microRNAs in Brassica oleracea infected with Xanthomonas campestris pv. campestris.

Abstract:

:Brassica oleracea var. capitata (cabbage) is an economically important crop affected by black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). MicroRNAs (miRNAs) play an important role in plant defense modulation and therefore the analysis of these molecules can help better understand plant-pathogen interactions. In this study, we report the differential expression of four miRNAs that seem to participate in the plant response to Xcc infection. Northern Blot and RT-qPCR techniques were used to measure miRNA expression in resistant (União) and susceptible (Kenzan) cultivars. From 6 miRNAs analyzed, 4 were detected and differentially expressed, showing a down- and upregulated expression profile in susceptible and resistant cultivars, respectively. These results suggest that miR156, miR167, miR169, and miR390 could play a role in B. oleracea resistance enhancement against Xcc and could be explored as potential resistance markers in B. oleracea-Xcc interaction.

journal_name

Mol Biol Rep

authors

Santos LS,Maximiano MR,Megias E,Pappas M,Ribeiro SG,Mehta A

doi

10.1007/s11033-019-04779-7

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

3523-3529

issue

3

eissn

0301-4851

issn

1573-4978

pii

10.1007/s11033-019-04779-7

journal_volume

46

pub_type

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