Abstract:
:The tertiary structure of nucleic acids results from an equilibrium between electrostatic interactions of phosphates, stacking interactions of bases, hydrogen bonds between polar atoms and water molecules. Water interactions with ribonucleic acid play a key role in its structure formation, stabilization and dynamics. We used high hydrostatic pressure and osmotic pressure to analyze changes in RNA hydration. We analyzed the lead catalyzed hydrolysis of tRNAPhe from S. cerevisiae as well as hydrolytic activity of leadzyme. Pb(II) induced hydrolysis of the single phosphodiester bond in tRNAPhe is accompanied by release of 98 water molecules, while other molecule, leadzyme releases 86.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Giel-Pietraszuk M,Barciszewski Jdoi
10.1007/s11033-012-1452-zsubject
Has Abstractpub_date
2012-05-01 00:00:00pages
6309-18issue
5eissn
0301-4851issn
1573-4978journal_volume
39pub_type
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