Hydrostatic and osmotic pressure study of the RNA hydration.

Abstract:

:The tertiary structure of nucleic acids results from an equilibrium between electrostatic interactions of phosphates, stacking interactions of bases, hydrogen bonds between polar atoms and water molecules. Water interactions with ribonucleic acid play a key role in its structure formation, stabilization and dynamics. We used high hydrostatic pressure and osmotic pressure to analyze changes in RNA hydration. We analyzed the lead catalyzed hydrolysis of tRNAPhe from S. cerevisiae as well as hydrolytic activity of leadzyme. Pb(II) induced hydrolysis of the single phosphodiester bond in tRNAPhe is accompanied by release of 98 water molecules, while other molecule, leadzyme releases 86.

journal_name

Mol Biol Rep

authors

Giel-Pietraszuk M,Barciszewski J

doi

10.1007/s11033-012-1452-z

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

6309-18

issue

5

eissn

0301-4851

issn

1573-4978

journal_volume

39

pub_type

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