Abstract:
:Rice production in recent years is highly affected by rapidly increasing temperatures in the tropical and sub-tropical countries, which threatens the sustainable production in near future. Hence, understanding the heat tolerance mechanism and evolving tolerant varieties is an immense need in the staple crop rice. An experiment has been conducted to identify differentially expressed genes in rice under heat stress conditions by employing a diverse set of 32 rice genotypes that includes reported heat tolerant genotypes Nagina 22 (N22) and Dular. Screening of the genotypes at field conditions during Summer-2018 for reproductive stage heat tolerance (wherein the mean minimum (29.8 °C) and maximum (38.4 °C) temperatures surpassed optimum temperatures (25 °C night/30 °C day) required for rice flowering and grain filling stages) and lab conditions employing thermal induction response (TIR) technique to know the genotype's acquired thermal tolerance revealed that the genotype FR13A (indica landrace) showed highest overall performance for multitude of traits viz., 95.29% of spikelet fertility (SF-%) at field level and 100% seedling survival percentage (SSP) at sub-lethal temperatures under laboratory conditions. The relative performance (under TIR) across all the three traits viz., relative shoot length (RSL) (4.91), relative root length (RRL) (equal to the control) and relative seedling dry weight (RSDW) (6.94) over control is high when compared to the other genotypes under study. However, the highly susceptible genotype PUSA1121 performed with 43.59 of SF%, 73.33% SSP, - 43.59 of RSL, - 36.02 of RRL over control. Hence, these contrasting genotypes were used for molecular analysis for identification of differentially expressed genes by employing 29 heat related gene specific primers. Five genes viz., OsGSK1, TT1, HSP70-OsEnS-45, OsHSP74.8 and OsHSP70 have shown differential expression between the two genotypes. Hence, the genotype FR13A, an 'indica' genotype, can be utilized in heat tolerance breeding programmes as donor parent in addition to the reported 'aus' genotypes, N22 and Dular. To our knowledge this is the first indica genotype identified for heat tolerance. The HSP70s, TT1 and OsGSK1 that proved with differential expression might be used for identification of gene specific InDels and thereby to develop functional markers that help in the marker assisted introgression breeding to develop heat tolerant varieties that can sustain production under dramatically changing climatic conditions.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Wahab MMS,Akkareddy S,Shanthi P,Latha Pdoi
10.1007/s11033-020-05291-zsubject
Has Abstractpub_date
2020-03-01 00:00:00pages
1935-1948issue
3eissn
0301-4851issn
1573-4978pii
10.1007/s11033-020-05291-zjournal_volume
47pub_type
杂志文章abstract::Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem cell (USSC) seems to be a suitable candidate. Recent studies have shown the involvement of microRNAs in se...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1591-2
更新日期:2012-07-01 00:00:00
abstract::Regulation of the sumoylation system at the level of gene expression has not yet been explored. To begin to define transcriptional regulatory features, the promoter region for the SUMO1 gene was cloned from human genomic DNA and characterized. Initially, a 532 base pair fragment upstream of and including the predicted...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9476-8
更新日期:2010-03-01 00:00:00
abstract::We report the characterization of VvIAA9, an auxin/indole-3-acetic acid (Aux/IAA) protein, in grapevine (Vitis vinifera L.). VvIAA9 was expressed abundantly in leaves and berries. VvIAA9 transcription was drastically upregulated from anthesis to onset of ripening (termed véraison), in which berry diameter rapidly incr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1625-9
更新日期:2012-07-01 00:00:00
abstract::Abnormally protein aggregation and deposition are key pathological features of ALS, which may related with dysfunctional cellular autophagy. In the current study, we found that, compared with wtSOD1 cells, serum starvation treatment resulted in significant higher percentage of apoptosis in mutSOD1 cells; Lithium treat...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05071-4
更新日期:2019-12-01 00:00:00
abstract::The Follicle stimulating hormone beta polypeptide (FSHβ) gene has been investigated as a candidate gene for litter size because of its role in maturation of small and medium follicles into ovulating large follicles. Genes coding follicle stimulating hormone have been found to have significant effects on litter size in...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3789-y
更新日期:2015-02-01 00:00:00
abstract::Vascular endothelial growth factor B (VEGF-B) was reported to be angiogenic, and it was considered as a neuroprotective agent in mouse retinal ganglion cells following optic nerve crush. Thus, it was necessary to investigate whether VEGF-B contributes to the process of retinal and choroidal neovascularization. We aime...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2917-4
更新日期:2014-02-01 00:00:00
abstract::Circular RNA (circRNA), a novel type of non-coding RNA that consists of a circular loop, has been demonstrated to act as a "sponge" for microRNAs (miRNAs). However, the role of circRNAs in keloid remains unknown. In this study, we investigated circRNA expression profiles in keloid to identify potential diagnostic and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05120-y
更新日期:2020-01-01 00:00:00
abstract::Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper alternative to more commonly used methods of producing transgenic animals. In this study, the capra semen ejaculates were pooled together and then incubated in vitro with DIG-labeled DNA. The binding and internalizing rates were observe...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1019-4
更新日期:2012-03-01 00:00:00
abstract::Several lines of evidence suggest that the ubiquitin-proteasome pathway is involved in sepsis-induced muscle catabolism. The gene expression of ubiquitin and several of the proteasome subunits was increased in muscle from both septic rats and patients. In other studies, the activity of isolated 20S proteasomes was sti...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1006916206260
更新日期:1999-04-01 00:00:00
abstract::CyclinD1 over-expression is the key pathogenetic event underlying airway smooth muscle (ASM) proliferation. Human antigen R (HuR) is a ubiquitously expressed RNA-binding protein, and is known to regulate the expression of multiple cell cycle regulators. The aim of the study is to investigate whether HuR might also be ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1392-z
更新日期:2012-05-01 00:00:00
abstract::Quinone oxidoreductases (NAD(P)H): quinone oxidoreductase 1 (NQO1) and NRH: quinone oxidoreductase 2 (NQO2) are an antioxidant enzyme, important in the detoxification of environmental carcinogens. Methylene-tetra-hydrofolate reductase (MTHFR), plays a role in folate metabolism and may have oncogenic role through disru...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2023-z
更新日期:2012-12-01 00:00:00
abstract::Proton beam therapy is widely used for treating brain tumor. Despite the efficacy of treatment, the use of this therapy has met some limitations associated with possible damage to normal brain tissues located beyond the tumor site. In this context, the exploration of the harmful effects of protons on the normal brain ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05491-7
更新日期:2020-06-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are implicated in atherosclerosis evolution into a coronary artery disease (CAD). They could be used as biomarkers for a predictive approach when they are studied simultaneously. We aim in our study to demonstrate prospectively in patients with history of CAD that MMPs level is linked ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04914-4
更新日期:2019-10-01 00:00:00
abstract::Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05992-5
更新日期:2020-12-01 00:00:00
abstract::In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via t...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0840-0
更新日期:2012-02-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) incidence is fast-growing especially in countries highly prevalent with viral hepatitis. Its poor prognosis has driven the research toward the discovery of sensitive markers for early detection. We investigated the usefulness of serum Transforming growth factor-beta1 (TGF-β1), Glypican-3...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2829-3
更新日期:2013-12-01 00:00:00
abstract::Hypertension is a risk factor for the cardiovascular diseases. Although, several drugs are used to treat hypertension, the success of the antihypertensive therapy is limited. Resveratrol decreases blood pressure in animal models of hypertension. This study researched the mechanisms behind the effects of resveratrol on...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3737-x
更新日期:2015-01-01 00:00:00
abstract::The disturbed immune homeostasis is involved in the pathogenesis of an array of autoimmune diseases like rheumatoid arthritis (RA). The adenosine monophosphate-activated protein kinase (AMPK) with a pivotal role in immunometabolism process, also plays a regulatory function in the immune system. This study aims to eval...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05078-x
更新日期:2019-12-01 00:00:00
abstract:AIM:To study the function of the prodomain of ADAM17 (TACE) and to develop an approach for interfering with inflammation processes. METHOD:The expression plasmids of the TACE ectodomain (T1300), prodomain (T591), signal peptide and prodomain (T648), full length (T2472), and the turncated TACE without prodomain (T57-T1...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9224-5
更新日期:2009-04-01 00:00:00
abstract::The purpose of our research is to elucidate whether oxLDL activates P2X7R in cultured human podocytes and if the activation of P2X7R leads to podocyte apoptosis. Additionally, we explore the underlying mechanism involved in podocyte apoptosis. Immortalized human podocytes were incubated with oxLDL (80 µg/ml), P2X7R an...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04823-6
更新日期:2019-08-01 00:00:00
abstract::Heparan sulfate proteoglycans (HSPGs) located at the cell surface and in the extracellular matrix of most animal tissues are proteoglycan coreceptors that carry heparan sulfate chains, and play a vital role in infections of many diseases. HSPGs are classified as glypican, syndecan, perlecan and agrin according to diff...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2340-2
更新日期:2013-03-01 00:00:00
abstract::Experiments with 5'-azacytidine and hematopoietic growth factor approved for the transformation of human mesenchymal cells into hematopoietic cells have demonstrated that cell fate can be dramatically altered by changing the epigenetic state of cells. Here, we demonstrate that umbilical cord-derived human mesenchymal ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3351-y
更新日期:2014-07-01 00:00:00
abstract::Using kinetics of approach to steady state labeling, we have found that liver poly(A)+ mRNAs fall into three populations, differing in stability and probably in processing, as reflected in their dissimilar delays in reaching polyribosomes and turnover times. There are mRNA-1 (delay 10 min, half-life 1 hr); mRNA-2(dela...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00357013
更新日期:1975-12-01 00:00:00
abstract::The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and coding regions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Compar...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0431-5
更新日期:2011-04-01 00:00:00
abstract::In vivo and in vitro expressed N-terminal sequence of EWS (EAD) and hsRPB7 (subunit of human RNA polymerase II) were probed for protein-protein interactions using pull-down assays. In result, it was found that the proteins 57Z (residues 1-57 of EAD) and hsRPB7 interact in vitro forming a stable complex. The direct int...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9308-2
更新日期:2009-07-01 00:00:00
abstract::One of the major limitations when attempting to obtain detailed biochemical, biophysical and immunological characterization of plant DNA mismatch repair proteins is their extremely low abundance in vivo under normal growth conditions. An initial analysis of PMS1 transcript level in various Arabidopsis thaliana tissues...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0203-2
更新日期:2011-02-01 00:00:00
abstract::Osteoporosis is a degenerative disease of the skeletal system, and its major complication is fracture that severely influences the living quality of the middle-aged and the aged. The purpose of this study was to investigate the significance of sex hormones and some biochemical indicators related to bone metabolism in ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0187-y
更新日期:2011-02-01 00:00:00
abstract::The treatment of anaplastic astrocytoma (AA) is controversial. New chemotherapeutic approaches are needed for AA treatment. Temozolomide (TMZ) is one of the chemotherapeutic drugs for the treatment of AA. The cytotoxic effects of TMZ can be removed by the MGMT (O(6)-methylguanine-DNA methyltransferase) enzyme. Then, c...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2908-5
更新日期:2014-02-01 00:00:00
abstract::The purpose of this study is to investigate the effect of H2O2 on the aging of melanogenesis in human melanocytes. The staining of SA-β-galactosidase, an aging marker, was remarkably increased in the cells aged with H2O2 at 62.5 µM or more compared with young cells. The intracellular H2O2 level and melanin synthesis w...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04708-8
更新日期:2019-04-01 00:00:00
abstract::Silky Fowl is a natural mutant with hyperpigmentation of various internal tissues. Although the mechanism of hyperpigmentation remains unclear, recent studies have shown that the abnormal migration of melanoblast and the absence of environmental barrier molecules are responsible for the hyperpigmentation in Silky Fowl...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0248-2
更新日期:2011-02-01 00:00:00