Abstract:
:A reduction in the number of functional β-cells is the central pathological event in diabetes. Liver and ventral pancreas differentiates simultaneously in the same general domain of cells within embryonic endoderm. In addition, the precursor cell population being bi-potential may be targeted for either pancreas or liver development. Hepatic stem cells were redirected in vivo to functional insulin producing cells in a acetylaminofluorene-partial hepatectomy (AAF/PH) adult male rat model with/without GLP-1 treatment. In routine H&E histology and immunohistochemistry, stem cells resembled β cells in GLP-1 injected rats. Immunoblots revealed involvement of adenylate cyclase, TLR4 and PDX1 in insulin synthesis. Expression of genes (GLP-1R, MAFA, PDX1, INS1 and INS2) augmented in the GLP-1 treated regenerated liver. Results strongly indicated the key role of GLP-1 in the induction of insulin secretion in trans-determined reprogrammed cell in vivo. The present method being vector free poses no risk of vector spillover in the host and holds promise.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Sarkar S,Munshi C,Chatterjee S,Mukherjee S,Bhattacharya Sdoi
10.1007/s11033-019-04870-zsubject
Has Abstractpub_date
2019-10-01 00:00:00pages
5501-5509issue
5eissn
0301-4851issn
1573-4978pii
10.1007/s11033-019-04870-zjournal_volume
46pub_type
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