Abstract:
:Anti-EGFR monoclonal antibodies (anti-EGFR MoAbs) in metastatic colorectal cancer (mCRC) treatment are still not effective in all patients. This study aimed to evaluate the relationship between BRAF V600E mutation and the tumor response of anti-EGFR MoAbs for first-line treatment in mCRC patients. We searched the MEDLINE and EMBASE databases, using the key words that included colorectal cancer, cetuximab, panitumumab, and BRAF mutation and retrieved 445 articles. Among them four were included in the systematic review. Relative risks (RRs) with 95% confidence intervals (CI) for response rate were calculated. BRAF mutation carriers had worse ORR than non-carriers in mCRC patients with KRAS wild-type in first-line treatment whether adding anti-EGFR MoAb to chemotherapy or not (RR = 0.43, [95% CI 0.16-0.75]; RR = 0.38, [95% CI 0.20-0.73]). But in the unselected patients whose KRAS mutation were unknown, BRAF mutation carriers had similar ORR whether adding cetuximab to chemotherapy or not (RR = 0.45, [95% CI 0.18-1.09]; RR = 0.57, [95% CI 0.15-2.23]). In BRAF mutation carriers adding anti-EGFR MoAb to chemotherapy was similar to chemotherapy alone whether in patients with wild-type KRAS or unselected patients (RR = 1.61, [95% CI 0.57-4.47]; RR = 0.71, [95% CI 0.18-2.77]). But in the BRAF mutation non-carriers, adding anti-EGFR MoAb produced a clear benefit in response rate than chemotherapy alone and this advantage was restricted to KRAS wild-type patients (RR = 1.48, [95% CI 1.28-1.71]). BRAF mutation decreases tumor response in first-line treatment whether cetuximab was given or not in patients with KRAS wild-type, and anti-EGFR MoAb produces a clear benefit in response rate in patients with BRAF and KRAS wild-type.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Cui D,Cao D,Yang Y,Qiu M,Huang Y,Yi Cdoi
10.1007/s11033-013-2974-8subject
Has Abstractpub_date
2014-03-01 00:00:00pages
1291-8issue
3eissn
0301-4851issn
1573-4978journal_volume
41pub_type
杂志文章,meta分析abstract::Rhodoleia Champion ex Hooker is one of the most primitive relict genera of Hamamelidaceae, a key family exploited to understand the origin and early evolution of flowering plants. Genomic simple sequence repeats (SSRs) were developed for R. championii to perform genetic diversity, phylogeographical structure or even s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05106-w
更新日期:2019-12-01 00:00:00
abstract::In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis-inducing protein, as a GST-fusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death. The HAP protein inhibited the bacterial growth within 30 min after inducting HAP expression. The transmission e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/b:mole.0000043551.84883.c8
更新日期:2004-09-01 00:00:00
abstract::Hepatitis B virus X protein (HBx) is closely involved in the development of hepatocellular carcinoma (HCC). Tumor suppressor p53 was reported to induce HBx degradation and repress its oncogenic function recently, but the molecular mechanism is unknown. In this study, we attempted to identify the underlying mechanism. ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9855-1
更新日期:2010-07-01 00:00:00
abstract::The cardiomyopathy associated 5 (CMYA5) gene was also called TRIM76, which was belonged to the tripartite motif super family of proteins (TRIM). It was a direct transcriptional target for MEF2A and it played an important role in myofibrillogenesis. In the present study, a 12056 bp cDNA sequence of the porcine CMYA5 ge...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0334-5
更新日期:2011-03-01 00:00:00
abstract::The new transcriptomes provided comprehensive sequence profiling data of transcriptomic variation during vernalization in Lily Asiatic Hybrids 'Tiny ghost'. A number of 52,277,184 sequencing raw reads totaling 5.11 Gbp of the chilling treatment (4 °C) sample and 39,466,176 sequencing raw reads totaling 3.85 Gbp of roo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3250-2
更新日期:2014-06-01 00:00:00
abstract::High Mobility Group Box 1 (HMGB1) is a nuclear protein participating in chromatin architecture and transcriptional regulation. Recently, there is increasing evidence that HMGB1 contributes to the pathogenesis of chronic inflammatory and autoimmune diseases due to its pro-inflammatory and immunostimulatory properties. ...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-009-9485-7
更新日期:2010-03-01 00:00:00
abstract::Epidemiological studies found inconsistent results on the association of two variants on TGFBR1 (TGFBR1*6A and Int7G24A) with colorectal cancer (CRC) risk. The present study was aimed to evaluate the association of these two variants with CRC susceptibility via the meta-analysis methods. For variant TGFBR1*6A, nine re...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-1009-6
更新日期:2012-03-01 00:00:00
abstract::In the post-genomic era, providing a detailed description of protein functions poses a formidable challenge. To gain functional insights, we have to construct many kinds of expression vectors. DNA recombination based on polymerase chain reaction (PCR) and digestion followed by ligation is the preferred method for vect...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9527-1
更新日期:2010-03-01 00:00:00
abstract::Plumbagin (PLB) is a phytochemical being used for centuries in traditional medicines. Recently, its capacity to inhibit the development of human tumors has been observed, through the induction of apoptosis, cell cycle arrest, and inhibition of angiogenesis and metastasis. Here we evaluated the mechanism of action of P...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05042-9
更新日期:2019-12-01 00:00:00
abstract::The aim of this study was to determine whether tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) promoter polymorphisms confer susceptibility to periodontitis in ethnically different populations. A literature search was performed using PubMed and Embase and a meta-analysis of the identified studies was conducte...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-013-2621-4
更新日期:2013-08-01 00:00:00
abstract::Of the only known two Lagoon populations of Irrawaddy dolphins (Orcaella) in the world, one is residing in the Chilika Lagoon in Orissa state, India. In addition to accidental deaths in gill net fishery and mechanized boat operations, there has been exploitation of the species for their oil. Extreme patchy distributio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0277-x
更新日期:2011-03-01 00:00:00
abstract::The glutathione S-transferase (GST) family of enzymes is known to play a pivotal role in phase II of biotransformation of xenobiotics, environmental carcinogens and pharmacological drugs. The objective of the present study was to investigate the role of GSTM1 and GSTT1 null genotypes as risk factors for chronic obstru...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0287-8
更新日期:2011-03-01 00:00:00
abstract::The interaction between ovalbumin (OVA) and three purine alkaloids (caffeine, theophylline and diprophylline) was investigated by the aid of intrinsic and synchronous fluorescence, ultraviolet-vis absorbance, resonance light-scattering spectra and three-dimensional fluorescence spectra techniques. Results showed that ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2418-x
更新日期:2013-04-01 00:00:00
abstract::20S proteasomes are large (700 kDa) proteinase complexes which form the central catalytic core of larger complexes (26S proteasomes or PA28-20S complexes) formed by association with regulatory particles. These larger complexes are involved in diverse regulatory processes in the cell including cyclin breakdown, proteol...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1006969517958
更新日期:1999-04-01 00:00:00
abstract::According to the stem cell theory for cancer, hepatocellular carcinomas are sustained by a group of cancer stem cells (CSCs) which are responsible for resistance to chemotherapy. In the present study we aimed to examine lipid metabolism in cancer stem cells induced by long-term treatment with sorafenib and its relatio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05352-3
更新日期:2020-04-01 00:00:00
abstract::Spermiogenesis is a developmental process undergoing continuous differentiation to drive a diploid spermatogonium towards a haploid sperm cell. This striking transformation from spermatogonium to spermatozoa is made possible by the stage-specific adaption of cytoskeleton and associated molecular motor proteins. KIFC1 ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1593-0
更新日期:2012-07-01 00:00:00
abstract::The aim of this study was to characterise the effects of ischemic preconditioning (IP) on heart function parameters (ΔST and ΔT), activities of serum creatine kinase (CK), lactate dehydrogenase (LDH), and levels of serum nitric oxide (NO), malondialdehyde (MDA), and myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor nec...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2677-1
更新日期:2013-10-01 00:00:00
abstract::We report here cloning and characterization of a novel human gene, termed VMP, which is a vesicular membrane protein. RT-PCR analysis shows that VMP is expressed exclusively in brain of the 16 tissues examined, suggesting that it is a neuron-specific membrane protein. The cDNA encodes 195 amino acid with a putative mo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1020402410522
更新日期:2002-09-01 00:00:00
abstract::Micro-RNAs (miRNAs) play key roles in regulating genes of the immune system. The aim of this study was to examine the expression of miR-1, 21 and 125a in the immune cells taken from the peripheral blood of patients suffering from chronic HBV infection (CHB). This cross-sectional study was performed on 60 CHB patients ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3355-7
更新日期:2014-07-01 00:00:00
abstract::Based on the internal transcribed spacer and inter-simple sequence repeats (ISSR), the phylogenetic relationship and genetic diversity of Metarhizium spp., pathogens found in Chinese burrower bugs, Schiodtella formosana, were analyzed. The results showed that the causative agents of the epizootic green muscardine dise...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2088-8
更新日期:2013-01-01 00:00:00
abstract::Chloroplast microsatellites for two Korean endemic species, Eranthis byunsanensis and E. pungdoensis (Ranunculaceae), were isolated to address the questions of their distributional patterns and evolutionary relationships, using next-generation sequencing. Twenty-four polymorphic chloroplast microsatellite markers for ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-017-4117-0
更新日期:2017-08-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is a world-wide health problem and its incidence accounts for 1.9-3.5% of all malignant tumors. Transforming growth factor beta/Smads (TGF-beta/Smads) signaling pathway plays an important role in oncogenesis, but its function and molecular mechanisms in OSCC remain unclear. Expressi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9256-x
更新日期:2009-05-01 00:00:00
abstract::A cDNA encoding an O-methyltransferase (namely FGCOMT1) was identified from the medicinal plant Trigonella foenum-graecum L. The FGCOMT1 enzyme is a functional caffeic acid O-methyltransferase (COMT) and is localized in the cytosol. Kinetic analysis indicated that FGCOMT1 protein exhibited the highest catalyzing effic...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0899-7
更新日期:2012-02-01 00:00:00
abstract::A chromosomal fragment containing purA, the nearest marker from the replication origin of the Bacillus subtilis chromosome, was highly purified as a complex containing at least proteins and being solubilized easily during cell lysis. The complex had a markedly higher sedimentation rate (70-120S) than the bulk of the s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00356932
更新日期:1976-07-01 00:00:00
abstract::Heterogeneous nuclear ribonucleoprotein particles (HnRNP) were separated in metrizamide density gradients, into two fractions migrating to 1.31 g ml-1 and 1.18 g ml-1, respectively. Proteins associated with each of these fractions were analysed by SDS-acrylamide gel electrophoresis. It is shown that the whole proteins...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00643481
更新日期:1977-03-01 00:00:00
abstract::Wnts are secreted lipid-modified signaling proteins. Activation of Wnt signalling in many tissues has also been associated with cancer. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this st...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9055-9
更新日期:2008-06-01 00:00:00
abstract::We investigated phenotypic variations for pod shattering, pod length and number of seeds per pod in large germplasm collections of Brassica juncea (2n = 36; AABB) and its progenitor species, B. rapa (2n = 20; AA) and B. nigra (2n = 16; BB). Pod shatter resistance was measured as energy required for rupturing a mature ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05384-9
更新日期:2020-04-01 00:00:00
abstract::The cyclin-dependent kinases (CDKs) are a family of serine/threonine kinases, playing an essential role in regulating cell-cycle progression. In our present work, human CDK5 and a novel CDK5 splicing variant, named as CDK5-SV, were cloned from the cDNA library of human testis. CDK5-SV lacking the exon 7 of CDK5 encode...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9752-7
更新日期:2010-06-01 00:00:00
abstract::Inflammatory cytokine, adipokine and adhesion molecules are known to play a key role in pathogenesis of diabetic kidney disease (DKD). In this study, our aim was to investigate the role of fetuin-A in relation with pro-inflammatory cytokines (IL-6, IL-18), adipokines (adiponectin, leptin), chemokine (MCP-1), and adhes...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04592-2
更新日期:2019-02-01 00:00:00
abstract::PNAS-4 is a novel pro-apoptotic protein activated during the early response to DNA damage; however, the molecular mechanisms and pathways regulating PNAS-4 expression in tumors are not well understood. We hypothesized that PNAS-4 is a p53 down-stream target gene and designed this study. We searched online for putative...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0732-3
更新日期:2012-01-01 00:00:00