Development and characterization of 24 chloroplast microsatellite markers for two species of Eranthis (Ranunculaceae).

Abstract:

:Chloroplast microsatellites for two Korean endemic species, Eranthis byunsanensis and E. pungdoensis (Ranunculaceae), were isolated to address the questions of their distributional patterns and evolutionary relationships, using next-generation sequencing. Twenty-four polymorphic chloroplast microsatellite markers for these two species were developed, and then characterized in 65 individuals (55 individuals of E. byunsanensis and 10 individuals of E. pungdoensis). The number of alleles per locus ranged from 2 to 9; the average number of alleles across all the loci scored 4.792. The unbiased diversity per locus ranged from 0.089 to 0.880; the unbiased diversity averaged over all the loci was 0.646. The developed markers were successfully amplified for three congeneric species, E. stellata, E. pinnatifida, and E. longistipitata. The markers developed in this study can provide a valuable and important tool for understanding genetic variations, population structures, evolutionary histories and phylogeography of E. byunsanensis, E. pungdoensis, and related species.

journal_name

Mol Biol Rep

authors

Oh A,Oh BU

doi

10.1007/s11033-017-4117-0

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

359-363

issue

4

eissn

0301-4851

issn

1573-4978

pii

10.1007/s11033-017-4117-0

journal_volume

44

pub_type

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