Abstract:
:We investigated phenotypic variations for pod shattering, pod length and number of seeds per pod in large germplasm collections of Brassica juncea (2n = 36; AABB) and its progenitor species, B. rapa (2n = 20; AA) and B. nigra (2n = 16; BB). Pod shatter resistance was measured as energy required for rupturing a mature dry pod, with a specially fabricated pendulum machine. Rupture energy (RE) ranged from 3.3 to 11.0 mJ in B. juncea. MCP 633, NR 3350 and Albeli required maximum energy to shatter a pod. It ranged from 2.5 to 7.8 mJ for B. rapa with an average of 5.5 mJ. B. nigra possessed easy to rupture pods. Correlation analysis showed strong associations among these traits in B. juncea and B. rapa. Genome wide association studies were conducted with select sets of B. juncea and B. rapa germplasm lines. Significant and annotated associations predict the role of FRUITFULL, MANNASE7, and NAC secondary wall thickening promoting factor (NST2) in the genetic regulation of shatter resistance in B. juncea. NST2 and SHP1 appeared important for pod length and seeds per pod in B. rapa. Candidate gene based association mapping also confirmed the role of SHP1 and NST2 in regulating pod shattering and related pod traits in B. rapa and B. juncea. Footprints of selection were detected in SHP1, SHP2 (B. rapa, B. nigra and B. juncea), RPL (B. rapa) and NAC (B. juncea). Our results provide insights into the genetic architecture of three pod traits. The identified genes are relevant to improving and securing crop productivity of mustard crop.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Kaur J,Akhatar J,Goyal A,Kaur N,Kaur S,Mittal M,Kumar N,Sharma H,Banga S,Banga SSdoi
10.1007/s11033-020-05384-9subject
Has Abstractpub_date
2020-04-01 00:00:00pages
2963-2974issue
4eissn
0301-4851issn
1573-4978pii
10.1007/s11033-020-05384-9journal_volume
47pub_type
杂志文章abstract::β-Catenin plays a crucial role in embryonic development and responds to the activation of several signal transduction pathways. In this paper, in order to understand the functions of β-catenin gene in early embryonic development of Artemia sinica, the complete cDNA sequence was cloned for the first time using RACE tec...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0788-0
更新日期:2012-01-01 00:00:00
abstract::Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In the present study, we report the identification and characterization of a crustin (CrusSp) from the hemocyte of mud crab, Scylla paramamosain using an expressed sequence tag (EST) and rapid amplific...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9253-0
更新日期:2009-05-01 00:00:00
abstract::Previous data on association between CYP2E1 Rsa I/Pst I polymorphism and oral cancer risk were controversial. To investigate the association between CYP2E1 Rsa I/Pst I polymorphism and oral cancer risk. We performed a meta-analysis to assess the relationship between oral cancer and genotype with English language until...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-0777-3
更新日期:2012-01-01 00:00:00
abstract::Increasing numbers of reports show that imprinted genes play a crucial role in fetal development, and uniparental embryos, which possess two paternally or two maternally derived pronuclei, are excellent tools for investigating the biological significance of imprinted genes. In the present study, to examine the in vitr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3774-5
更新日期:2015-02-01 00:00:00
abstract::A full-length cDNA encoding wheat peroxisomal ascorbate peroxidase (pAPX) was cloned by Suppression Subtractive Hybridization (SSH) and in silico approach. The cDNA was 1027 bp in length and contained a complete ORF of 876 bp, which encodes a protein of 292 amino acid residues. Its deduced amino acids sequence had 84%...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-005-4536-1
更新日期:2006-09-01 00:00:00
abstract::Amerindians immigrated to Madrid (Spain) region are about 10% of the present day population. Amerindians are believed to be the first American inhabitants, before Na-Dene speakers, Aleuts and Eskimo. They may initially have arrived to America from Siberia and also from other parts of Pacific Sea (South Asia, Polynesia...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0357-y
更新日期:2011-04-01 00:00:00
abstract::The cyclin-dependent kinases (CDKs) are a family of serine/threonine kinases, playing an essential role in regulating cell-cycle progression. In our present work, human CDK5 and a novel CDK5 splicing variant, named as CDK5-SV, were cloned from the cDNA library of human testis. CDK5-SV lacking the exon 7 of CDK5 encode...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9752-7
更新日期:2010-06-01 00:00:00
abstract::Studies of the refractive properties of Gramicidin A in absolute ethanol and in ethanol water mixtures showed that this peptide in solution undergoes a conformational transition resulting in species with different refractivity. Accordingly, the folded form of this peptide shows a lower specific refractive index increm...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00357540
更新日期:1975-07-01 00:00:00
abstract::Developing gene transfer technologies enables the genetic manipulation of the living organisms more efficiently. The methods used for gene transfer fall into two main categories; natural and artificial transformation. The natural methods include the conjugation, transposition, bacterial transformation as well as phage...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-020-05343-4
更新日期:2020-04-01 00:00:00
abstract::RNA polymerase III in an extract of HeLa cells transcribes RNA 5S in size from genomic bovine DNA template. This RNA represents a major fraction of the RNA synthesized. 5S RNA is not transcribed from bovine chromatin at equivalent concentrations and RNA the size of tRNA or tRNA precursor is not detected. At low UTP co...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775362
更新日期:1983-08-01 00:00:00
abstract::Endometriosis is a polygenic and multifactorial gynecology situation which might be associated with angiogenesis. In the current study we assess the role of vascular endothelial growth factor (VEGF) - 2578 A/C, and + 936 C/T polymorphisms in susceptibility to endometriosis and checking the expression of VEGF mRNA in e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04807-6
更新日期:2019-06-01 00:00:00
abstract::This experiment was conducted to explore the biological functions of myogenin (MyoG) gene. MyoG gene was cloned from genome of Hu sheep by overlap extension PCR. Then, pEGFP-C1-MyoG and pcDNA3.0-MyoG fusion expression vectors was constructed and pEGFP-C1-MyoG vector had been transfected into NIH-3T3 cells by liposomes...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2945-0
更新日期:2014-02-01 00:00:00
abstract::The quantitative changes of double-stranded RNA components of nuclear ribonucleo-protein particles containing pre-mRNA was investigated in the course of incubation of particles at 37 degrees C. The incubation of purified nuclear particles revealed the fragmentation of long double-stranded RNA sequences into shorter st...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00423235
更新日期:1976-11-01 00:00:00
abstract::The potential benefiting effects of probiotic Dahi on age-inflicted accumulation of oxidation products, antioxidant enzymes and expression of biomarkers of ageing were evaluated in mice. Probiotic Dahi were prepared by co-culturing in buffalo milk (3% fat) Dahi bacteria (Lactococcus lactis ssp. cremoris NCDC-86 and La...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0920-1
更新日期:2012-02-01 00:00:00
abstract::We have cloned two full-length cDNAs from two ferritin genes (Aifer1 and Aifer2) of the bay scallop, Argopecten irradians (Lamarck 1819). The cDNAs are 1,019 and 827 bp in length and encode proteins of 171 and 173 amino acids, respectively. The 5' UTR of each contains a conserved iron response element (IRE) motif. Seq...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0339-0
更新日期:2011-03-01 00:00:00
abstract::This study was designed to identify the differential expression of the canonical transient receptor potential (TRPC) channels in the left ventricle of spontaneously hypertensive rats (SHR). Echocardiography studies were performed to compare the left ventricular function in SHR vs. Wistar-Kyoto rats (WKY), and the mRNA...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9792-z
更新日期:2010-07-01 00:00:00
abstract::MADS-box genes encode important transcription factors in plants that are involved in many processes during plant growth and development. An investigation of the soybean genome revealed 106 putative MADS-box genes. These genes were classified into two classes, type I and type II, based on phylogenetic analysis. The soy...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2438-6
更新日期:2013-06-01 00:00:00
abstract::Kawasaki disease (KD) is a multi-systemic vasculitis which preferentially affects infants and children. A single nucleotide polymorphism (rs28493229) in the inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) was identified to be associated with the increased risk of KD; however, in more recent studies associations have b...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-2022-0
更新日期:2012-12-01 00:00:00
abstract::In a meritorious effort H. de Rothschild compiled in 1899 all publications on mammary gland development and milk--a grand total of 8375 [1]. In the preface to this publication Duclaux states: 'Such a discrepancy between the tremendous efforts and the paltriness of the results--hundreds of scientists and thousands of r...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1006851531360
更新日期:1997-08-01 00:00:00
abstract::Purified cucumber mosaic virus (CMV) RNA was reacted with NaIO4 and then [3H]KBH4 to label ends containing exposed 2',3'-hydroxyls. The four major RNA species were then fractionated by polyacrylamide gel electrophoresis. Analysis of digests of these RNAs by ribonucleases T1 plus T2, alkaline phosphatease, snake venom ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00357014
更新日期:1975-12-01 00:00:00
abstract::With an aim at detecting the ultra-low concentration of avian influenza virus (AIV), a highly sensitive hybrid assay based on immunology and polymerase chain reaction was developed. The TopYield microtiter plates were coated with ten-fold serial dilutions of H5N1 subtype AIV ranging from 10 EID(50 )ml(-1)~10(-4) EID(5...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0315-8
更新日期:2011-03-01 00:00:00
abstract::Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional requirements during periods of reproductive activity. Therefore, a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0666-1
更新日期:2011-11-01 00:00:00
abstract::It is well-established that an impaired adipose tissue function and morphology caused by a dysregulated gene expression contribute substantially to obesity. Nowadays, animal model studies and in vitro surveys provide evidence for possible roles of HDACs as emerging epigenetic players in the pathogenesis of obesity. Ho...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05431-5
更新日期:2020-05-01 00:00:00
abstract::Epidemiological studies found inconsistent results on the association of two variants on TGFBR1 (TGFBR1*6A and Int7G24A) with colorectal cancer (CRC) risk. The present study was aimed to evaluate the association of these two variants with CRC susceptibility via the meta-analysis methods. For variant TGFBR1*6A, nine re...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-1009-6
更新日期:2012-03-01 00:00:00
abstract::The Great-billed Seed-finch, Sporophila maximiliani, is a threatened neotropical bird that has declined mainly due to illegal trapping, with very few records in the wild in the last two decades. Despite the existence of a considerable captive population that could be used for reintroductions into the wild, many indivi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4377-3
更新日期:2018-12-01 00:00:00
abstract::The purpose of our research is to elucidate whether oxLDL activates P2X7R in cultured human podocytes and if the activation of P2X7R leads to podocyte apoptosis. Additionally, we explore the underlying mechanism involved in podocyte apoptosis. Immortalized human podocytes were incubated with oxLDL (80 µg/ml), P2X7R an...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04823-6
更新日期:2019-08-01 00:00:00
abstract::This study investigated the role of the mevalonate pyrophosphate decarboxylase gene in the triterpene biosynthetic pathway of Ganoderma lucidum. The mevalonate pyrophosphate decarboxylase gene (mvd) was isolated using a degenerate primer-PCR technique. An analysis of the Gl-mvd transcription profile revealed a positiv...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1431-9
更新日期:2012-05-01 00:00:00
abstract::Published data regarding the association between the apurinic/apyrimidinic endonuclease 1 (APE1) Asp148Glu polymorphism and bladder cancer risk showed inconclusive results. This meta-analysis of literatures was performed to draw a more precise estimation of the relationship. We systematically searched PubMed, Embase, ...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-2046-5
更新日期:2013-01-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is an autoimmune disease, with multiple genetic and environmental factors involving in its etiology. Angiotensin converting enzyme (ACE) gene was reported to have important roles in the development and progression of SLE. In this study, a case-control study was carried out to investi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1813-7
更新日期:2012-10-01 00:00:00
abstract::The interleukin (IL)-1β-511 C/T polymorphism has been shown to be functional and to contribute to the risk of gastric cancer. However, the relationship between the IL-1β-511 C/T polymorphism and gastric carcinogenesis remains inconclusive. A systematical electronic search was conducted of the MEDLINE, EMBASE, and CENT...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-014-3748-7
更新日期:2015-01-01 00:00:00