Abstract:
:The quantitative changes of double-stranded RNA components of nuclear ribonucleo-protein particles containing pre-mRNA was investigated in the course of incubation of particles at 37 degrees C. The incubation of purified nuclear particles revealed the fragmentation of long double-stranded RNA sequences into shorter stretches. The presence of nuclear sap in the incubation mixture resulted in degradation of the double-stranded RNAs into acid soluble products. Autodegradation and/or ribonuclease treatment of nuclear RNP particles is accompanied by quantitative changes in the minor protein constituents of informofer.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Molnar J,Samarina OPdoi
10.1007/BF00423235keywords:
subject
Has Abstractpub_date
1976-11-01 00:00:00pages
195-202issue
2eissn
0301-4851issn
1573-4978journal_volume
3pub_type
杂志文章abstract::Substantial epidemiological studies have shown an association of obesity with the common gynecological malignancy, endometrial cancer. The relevant interactions and contribution of estradiol and the adipose cytokine, leptin, in endometrial lesions are not completely understood. Suitable animal models to understand the...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05116-8
更新日期:2020-01-01 00:00:00
abstract::Ribosomes were isolated from baby hamster kidney fibroblasts, either 20 min or 2 days after labelling with radioactive amino acids, and their proteins subjected to two-dimensional polyacrylamide gel electrophoresis. No significant differences were observed between the amounts of radioactivity associated with the posit...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00777524
更新日期:1980-12-31 00:00:00
abstract::β-Catenin plays a crucial role in embryonic development and responds to the activation of several signal transduction pathways. In this paper, in order to understand the functions of β-catenin gene in early embryonic development of Artemia sinica, the complete cDNA sequence was cloned for the first time using RACE tec...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0788-0
更新日期:2012-01-01 00:00:00
abstract::Interleukin 15 (IL-15) is a Th1-related cytokine that triggers inflammatory cell recruitment with implications for pathogenesis in ulcerative colitis. The IL-15 gene is located within a 35 kb region of the q28-31 locus of chromosome 4. In the present work, the role of IL-15 gene polymorphisms as susceptibility markers...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3067-z
更新日期:2014-01-01 00:00:00
abstract::The present study aimed to obtain analgesic-antitumor peptide (AGAP) gene expression in plants. The analgesic-antitumor peptide (AGAP) gene was from the venom of Buthus martensii Karsch. Previous studies showed that AGAP has both analgesic and antitumor activities, suggesting that AGAP would be useful in clinical situ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9277-5
更新日期:2009-05-01 00:00:00
abstract::AP2/ERF transcription factors play an important role in regulation of the cross-talk between ethylene and jasmonate signaling pathways mediating defense responses of plants to biotic and abiotic stresses. In this study, an AP2/ERF transcription factor gene was isolated and characterized from laticifers of rubber tree ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1146-y
更新日期:2012-04-01 00:00:00
abstract::One of the major limitations when attempting to obtain detailed biochemical, biophysical and immunological characterization of plant DNA mismatch repair proteins is their extremely low abundance in vivo under normal growth conditions. An initial analysis of PMS1 transcript level in various Arabidopsis thaliana tissues...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0203-2
更新日期:2011-02-01 00:00:00
abstract::Whole-exome sequencing (WES) is an ideal method for the diagnosis of autosomal recessive diseases. The aim of this study was to evaluate the diagnostic power of WES in patients with autosomal recessive inheritance and to determine the relationship between genotype and phenotype. Retrospective screenings of 24 patients...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4470-7
更新日期:2019-02-01 00:00:00
abstract::Type 2 diabetes is one of the most prevalent diseases, which increases resistance to insulin in target tissues. The measurement of miRNAs quantity is a molecular approach for diagnosis of diabetes. miRNAs are small non-coding RNA strings of 21-23 long nucleotides that act as inhibitors in proteins translation. Several...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04959-5
更新日期:2019-10-01 00:00:00
abstract::The cyclin-dependent kinases (CDKs) are a family of serine/threonine kinases, playing an essential role in regulating cell-cycle progression. In our present work, human CDK5 and a novel CDK5 splicing variant, named as CDK5-SV, were cloned from the cDNA library of human testis. CDK5-SV lacking the exon 7 of CDK5 encode...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9752-7
更新日期:2010-06-01 00:00:00
abstract::Dyggve melchior clausen syndrome (DMC, MIM 223800) is a very rare autosomal recessive form of skeletal dysplasia associated with various degrees of mental retardation. It is characterized by a progressive spondyloepimetaphyseal dysplasia (SEMD) with disproportionate short stature, generalized platyspondyly and lacy il...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05774-z
更新日期:2020-09-01 00:00:00
abstract::Body measurement and meat quality traits which play important roles in the assessment of productivity and economy in cattle were influenced by genes and environmental factors. Latest studies showed that LYR motif containing 1 (LYRM1) may be involved in influencing fatness deposition in animals. The objective of this s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2195-6
更新日期:2013-02-01 00:00:00
abstract::Growth hormone-releasing hormone receptor (GHRHR) has important functions in the regulation of the growth hormone axis and the development and proliferation of pituitary somatotropes. Moreover, some mutations in mouse GHRHR can induce the dwarfism. The objective of this paper is to reveal the association of GHRHR with...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1927-y
更新日期:2012-12-01 00:00:00
abstract::A number of regions designated as RD1-RD16 (region of difference) and encompassing 129 open reading frames have been identified between Mycobacterium tuberculosis and Mycobacterium bovis on the one hand and Bacillus Calmette-Guérin on the other. Identification of T cell epitopes from this set of proteins may serve to ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9613-4
更新日期:2010-04-01 00:00:00
abstract::Rat liver chromatin activated by partial hepatectomy is more susceptible to the action of DNAse I than control chromatin isolated from intact liver. The study on the transfer of chromatin material to the acid-soluble fraction reveals a higher rate of activated chromatin degradation. Activated chromatin shows also an i...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00782891
更新日期:1979-12-31 00:00:00
abstract::A method is described which demonstrates the posibility of visualizing protein-complexed DNA by using pronase for both deproteinization and spreading of DNA for electron microscopy. The results show that proteolytic digestion is complete even under conditions of short formaldehyde fixation and pronase is an excellent ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00778415
更新日期:1979-08-31 00:00:00
abstract::Allelic variation of BAT-25 (a 25-repeat quasimonomorphic poly T) and BAT-26 (a 26-repeat quasimonomorphic polyA) loci as two mononucleotide microsatellite markers, were analyzed with high-performance liquid chromatography (HPLC) compared with Real-Time PCR using hybridization probes. BAT-26 and BAT-25 markers were us...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3138-1
更新日期:2014-05-01 00:00:00
abstract::Uterine leiomyomas (UL) are prevalent benign tumors, especially among women of African ancestry. The disease also has genetic liability and is influenced by risk factors such as hormones and obesity. This study investigates the haplotypes of the Cytochrome P450 1B1 gene (CYP1B1) related to hormones and coiled-coil dom...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04790-y
更新日期:2019-06-01 00:00:00
abstract::Alteration of the acrylamide:bisacrylamide ratio in the SDS-polyacrylamide gel used for Western blotting strongly improved the unambiguous detection of antibodies against 50-60 kDa autoantigens present in autoimmune patient sera. The relative migration of Ro52, the 56K autoantigen and calreticulin increased with reduc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00419667
更新日期:1992-09-01 00:00:00
abstract::Intrinsic bent DNA sites were identified in the 4289 bp segment encompassing the replication zone which directs DNA amplification and transcription of the C3-22 gene of Rhynchosciara americana. Restriction fragments showed reduced electrophoretic mobility in polyacrylamide gels. The 2D modeling of the 3D DNA path and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-006-0009-4
更新日期:2006-03-01 00:00:00
abstract::Hepatitis B virus X protein (HBx) is closely involved in the development of hepatocellular carcinoma (HCC). Tumor suppressor p53 was reported to induce HBx degradation and repress its oncogenic function recently, but the molecular mechanism is unknown. In this study, we attempted to identify the underlying mechanism. ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9855-1
更新日期:2010-07-01 00:00:00
abstract::Accurate normalization of gene expression with qRT-PCR depends on the use of appropriate reference genes (RGs) for the species under a given set of experimental conditions. Multiple RGs for gene expression analysis of soybean exposed to heavy metal stress treatment have not been reported in the literature. In this stu...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0897-9
更新日期:2012-02-01 00:00:00
abstract::The difference in sensitivity of polyphenylalanine synthesis in extracts from Artemia salina and rabbit reticulocytes to inhibition by the Phytolacca americana protein (PAP) has been found to be linked to the source of the supernatant enzyme fraction and not the ribosomes. In the presence of reticulocyte supernatant e...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00366257
更新日期:1976-04-01 00:00:00
abstract::Recently a GWAS study had identified 38 genomic variants commonly found in humans that influence migraine risk. For further replicate these findings, we selected two SNPs; rs2651899 on chromosome 1p36.32 in PRDM16 gene and rs10166942 on chromosome 2q37.1 close to TRPM8 gene for their associations with migraine in the ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04593-1
更新日期:2019-02-01 00:00:00
abstract::The blue swimming crab (Portunus pelagicus) is a valuable marine fishery resource in Indo-West Pacific Ocean. So far, rare genetic resource of this species is available. In this report, the restriction-site associated DNA (RAD) approach was employed to mine the genomic information and identify molecular markers in P. ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4339-9
更新日期:2018-12-01 00:00:00
abstract::Methyltransferases play essential roles in modulating important cellular and metabolic processes. A mouse putative N6-DNA methyltransferase gene (GenBank No AY456393) is a novel gene named mN6amt1(mN6A1). To investigate its function in cell fate and protein translation, RNA interference (RNAi)-mediated knock-down meth...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9243-2
更新日期:2009-04-01 00:00:00
abstract::Osteoporosis is a common health problem in Mexico, so it is essential to investigate the status of different gene polymorphisms that could serve as genetic susceptibility markers in the Mexican population. Genes with a role in bone metabolism are excellent candidates for association studies. In this study were determi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2357-6
更新日期:2013-03-01 00:00:00
abstract::Random amplified polymorphic DNA (RAPD) and microsatellite markers were applied to evaluate the genetic variation in endemic and endangered yellow catfish, Horabagrus brachysoma sampled from three geographic locations of Western Ghat, South India river systems. In RAPD, of 32 10-mer RAPD primers screened initially, 10...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9381-6
更新日期:2009-09-01 00:00:00
abstract::In the present study, we characterized an evolutionarily conserved non-transmembrane ATP-binding cassette protein: hABCF3. Subcellular immunofluorescence staining demonstrated that hABCF3 localizes preferentially in cytoplasm, unlike its paralog protein hABCF1, which localizes in both cytoplasm and nucleus. Quantitati...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2679-z
更新日期:2013-10-01 00:00:00
abstract::The purpose of this study was to assess the value of cytokeratin 19 (CK19) and matrix metalloproteinase 2 (MMP-2) in predicting lymph node metastasis (LNM) and survival after curative resection in hepatocellular carcinoma (HCC) patients. Expression of CK19 and MMP-2 in tumor tissue was assessed through immunohistochem...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0463-x
更新日期:2011-06-01 00:00:00