Refined definition of the 56K and other autoantigens in the 50-60 kDa region.

Abstract:

:Alteration of the acrylamide:bisacrylamide ratio in the SDS-polyacrylamide gel used for Western blotting strongly improved the unambiguous detection of antibodies against 50-60 kDa autoantigens present in autoimmune patient sera. The relative migration of Ro52, the 56K autoantigen and calreticulin increased with reduced acrylamide:bisacrylamide ratios in contrast to that of Ro60, La and Jo-1. These analyses indicated that these six autoantigens correspond to six distinct polypeptides. Further analyses using recombinant calreticulin showed that (i) the 56K autoantigen is neither identical nor related to calreticulin and (ii) calreticulin is not a Ro autoantigen. A series of experiments designed to better characterize the 56K autoantigen showed that (i) the antigen is not detectable in fixed cells, presumably due to masking of the epitopes; (ii) about equal amounts of the antigen were recovered in nuclear and cytoplasmic cell fractions after enucleation of the cells; (iii) the 56K autoantigen is not stably associated with either RNA or other proteins.

journal_name

Mol Biol Rep

authors

Pruijn GJ,Bozic B,Schoute F,Rokeach LA,van Venrooij WJ

doi

10.1007/BF00419667

keywords:

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

267-76

issue

4

eissn

0301-4851

issn

1573-4978

journal_volume

16

pub_type

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