Abstract:
:Rat liver chromatin activated by partial hepatectomy is more susceptible to the action of DNAse I than control chromatin isolated from intact liver. The study on the transfer of chromatin material to the acid-soluble fraction reveals a higher rate of activated chromatin degradation. Activated chromatin shows also an increased capacity for ethidium bromide (EB) binding as estimated from the isotherms of adsorption. The difference in EB binding between activated and control chromatin is abolished after DNAse I treatment. Conditions of mild digestion with DNAse I have been found under which the number of binding sites for EB per nucleotide decreases to almost the same level in activated and non-activated chromatin. The results suggest a preferential degradation of those DNA sequences in activated chromatin that are responsible for the increase in the ligand binding.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Batova IN,Fedoseeva GE,Zelenin AVdoi
10.1007/BF00782891subject
Has Abstractpub_date
1979-12-31 00:00:00pages
215-9issue
4eissn
0301-4851issn
1573-4978journal_volume
5pub_type
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