Increased susceptibility of activated rat liver chromatin to DNAse I.

Abstract:

:Rat liver chromatin activated by partial hepatectomy is more susceptible to the action of DNAse I than control chromatin isolated from intact liver. The study on the transfer of chromatin material to the acid-soluble fraction reveals a higher rate of activated chromatin degradation. Activated chromatin shows also an increased capacity for ethidium bromide (EB) binding as estimated from the isotherms of adsorption. The difference in EB binding between activated and control chromatin is abolished after DNAse I treatment. Conditions of mild digestion with DNAse I have been found under which the number of binding sites for EB per nucleotide decreases to almost the same level in activated and non-activated chromatin. The results suggest a preferential degradation of those DNA sequences in activated chromatin that are responsible for the increase in the ligand binding.

journal_name

Mol Biol Rep

authors

Batova IN,Fedoseeva GE,Zelenin AV

doi

10.1007/BF00782891

subject

Has Abstract

pub_date

1979-12-31 00:00:00

pages

215-9

issue

4

eissn

0301-4851

issn

1573-4978

journal_volume

5

pub_type

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