ASYMMETRIC LEAVES2 gene, a member of LOB/AS2 family of Arabidopsis thaliana, causes an abaxializing leaves in transgenic cockscomb.

Abstract:

:The leaf primordium derives from the peripheral zone of shoot apical meristem. During the formation of leaf primordia, they need to establish the proximodistal, mediolateral, and ab/adaxial axes. Among these axes, the ab/adaxial axis might be the most important. ASYMMETRIC LEAVES2 (AS2) gene is a member of AS2/LATERAL ORGAN BOUNDARY (LOB) family of Arabidopsis thaliana. In this work, we transformed 35S:AS2 transgene constructs to cockscomb (Celosia cristata) via Agrobacterium tumefaciens. All primary transformants subsequently obtained were placed into phenotypic categories and self-pollinated. As a whole, a total of 44 T1 35S:AS2 cockscomb plants obtained were grouped into two major categories: (I) slightly wrinkled leaves (28/44), (II) extremely curved leaves (16/44), on the basis of their leaf phenotypes. Furthermore, we characterized the anatomical features of these malformed leaves; and found the transformation of adaxial cell types into abaxial cell ones. A series of data suggest that AS2 might be involved in the determination of abaxial polarity in cockscomb plants. However, a few research teams have reported that AS2 might be involved in the determination of adaxial polarity in leaf primodia of Arabidopsis thaliana. These data above indicate that the roles of the same ab/adaxial determinant might differ between distinct species. At last, the different function of AS2 in distinct species was discussed.

journal_name

Mol Biol Rep

authors

Sun SB,Song JP,Meng LS

doi

10.1007/s11033-011-1288-y

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

4927-35

issue

4

eissn

0301-4851

issn

1573-4978

journal_volume

39

pub_type

杂志文章
  • The complete mitochondrial genome of Deracantha onos (Orthoptera: Bradyporidae).

    abstract::The complete mitochondrial genome 15,650 bp in size of the Deracantha onos has been determined. The gene content, base composition and codon usage of D. onos are coincident to typical hexapods mitochondrial genomes. Genes arrangement of D. onos is identical to Gryllotalpa orientalis, Ruspolia dubia and Anabrus simplex...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-007-9145-8

    authors: Zhou Z,Huang Y,Shi F,Ye H

    更新日期:2009-01-01 00:00:00

  • Partial molecular cloning, characterization, and analysis of the subcellular localization and expression patterns of the porcine OTUB1 gene.

    abstract::The product of the OTUB1 gene is a member of the OTU superfamily of predicted cysteine proteases and inhibits cytokine gene transcription via its interaction with a ubiquitin protease and E3 ubiquitin ligase. To further understand the functions of the porcine OTUB1 gene, the subcellular localization of porcine OTUB1 p...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-008-9353-x

    authors: Shan TL,Tang ZL,Guo DZ,Yang SL,Mu YL,Ma YH,Guan WJ,Li K

    更新日期:2009-07-01 00:00:00

  • The effect of chemical carcinogenesis on rat glutathione S-transferase P1 gene transcriptional regulation.

    abstract::To investigate mechanisms of rat glutathione S-transferase P1 gene (rGSTP1) expression regulation during chemical carcinogenesis. we studied enhancer elements located in the region between -2.5 kb to -2.2 kb. The region was upstream from the start site of transcription and was divided into two major fragments, GPEI an...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/a:1011993604051

    authors: Liu D,Liao M,Zuo J,Henner WD,Fan F

    更新日期:2001-03-01 00:00:00

  • Effects of vascular endothelial growth factor B on proliferation and migration in EA.Hy926 cells.

    abstract::Vascular endothelial growth factor B (VEGF-B) was reported to be angiogenic, and it was considered as a neuroprotective agent in mouse retinal ganglion cells following optic nerve crush. Thus, it was necessary to investigate whether VEGF-B contributes to the process of retinal and choroidal neovascularization. We aime...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2917-4

    authors: Zhang GH,Qin R,Zhang SH,Zhu H

    更新日期:2014-02-01 00:00:00

  • Growth inhibition induced by transforming growth factor-beta1 in human oral squamous cell carcinoma.

    abstract::Oral squamous cell carcinoma (OSCC) is a world-wide health problem and its incidence accounts for 1.9-3.5% of all malignant tumors. Transforming growth factor beta/Smads (TGF-beta/Smads) signaling pathway plays an important role in oncogenesis, but its function and molecular mechanisms in OSCC remain unclear. Expressi...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-008-9256-x

    authors: Wang X,Sun W,Bai J,Ma L,Yu Y,Geng J,Qi J,Shi Z,Fu S

    更新日期:2009-05-01 00:00:00

  • Postribosomal complexes containing eukaryotic initiation factor eIF-2.

    abstract::Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with othe...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00777498

    authors: Amesz H,Haubrich T,Voorma HO

    更新日期:1979-05-31 00:00:00

  • Role of Spata34 in cell proliferation and its expression pattern in postnatal development of rat testis.

    abstract::Spata34 is a testis-specific-expressed gene which exerts diverse functions in testis development. This study intends to examine the expression profiles of Spata34 in postnatal rat testis, and explore its potential roles in cell proliferation in vitro. We found that the mRNA and protein expression levels of Spata34 wer...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4439-6

    authors: Nie D,Li H,Liu Y,Liao Z

    更新日期:2018-12-01 00:00:00

  • Proto-oncogene Zbtb7a represses miR-125a-5p transcription in hepatocellular carcinoma cells.

    abstract::Zbtb7a is a transcription factor whose dysfunction is correlated to the development of several types of cancer, including hepatocellular carcinoma (HCC). It generally acts as a repressor of transcription downregulating the expression of several target genes including oncosuppressors ARF and Rb. In this study, Zbtb7a w...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05512-5

    authors: Di Palo A,Siniscalchi C,Mosca N,Russo A,Potenza N

    更新日期:2020-06-01 00:00:00

  • The phosphorylation of protein S6 in the newly-synthesized cytoplasmic ribosomes of hamster fibroblasts.

    abstract::Ribosomes were isolated from baby hamster kidney fibroblasts, either 20 min or 2 days after labelling with radioactive amino acids, and their proteins subjected to two-dimensional polyacrylamide gel electrophoresis. No significant differences were observed between the amounts of radioactivity associated with the posit...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00777524

    authors: Kennedy IM,Leader DP

    更新日期:1980-12-31 00:00:00

  • Current insight into the roles of microRNA in vitiligo.

    abstract::Vitiligo is a common chronic depigmented skin disease characterized by melanocyte loss or dysfunction in the lesion. The pathogenesis of vitiligo has not been fully clarified. Most studies have suggested that the occurrence and progression of vitiligo are due to multiple factors and gene interactions in which noncodin...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1007/s11033-020-05336-3

    authors: Yan S,Shi J,Sun D,Lyu L

    更新日期:2020-04-01 00:00:00

  • Unfolding Nrf2 in diabetes mellitus.

    abstract::In spite of much awareness, diabetes mellitus continues to remain one of major reasons for mortality and morbidity rate all over the globe. Free radicals cause oxidative stress which is responsible for causing diabetes. The recent advancements in elucidation of ARE/keap1/Nrf2 pathway can help in better understanding o...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1007/s11033-020-06081-3

    authors: Behl T,Kaur I,Sehgal A,Sharma E,Kumar A,Grover M,Bungau S

    更新日期:2021-01-03 00:00:00

  • Rapid development of 56 novel microsatellite markers for the benthic freshwater bug Aphelocheirus aestivalis using Illumina paired-end sequencing data and M13-tailed primers.

    abstract::The freshwater true bug Aphelocheirus aestivalis (Aphelocheiridae) is widely distributed in Europe but occurs rather locally and often in isolated populations. Moreover, it is threatened with extinction in parts of its range. Unfortunately, little is known about the genetic diversity and population structure due to th...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05974-7

    authors: Kaczmarczyk-Ziemba A

    更新日期:2020-12-01 00:00:00

  • Identification of the tree shrew ATP-binding cassette transporter A1 (ABCA1) and its expression in tissues : cDNA sequence and expression of tree shrew ABCA1.

    abstract::ATP-binding cassette transporter A1 (ABCA1) modulates plasma levels of high density lipoprotein (HDL), a cardiovascular protecting factor. Tree shrew was considered to be an animal protected from atherosclerosis characterized by high proportion of HDL in plasma. The cDNA clones and expression of tree shrew ABCA1 was i...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-007-9199-7

    authors: Zeng WW,Zhou B,Liu HR,Sun GT,Chen BS

    更新日期:2009-03-01 00:00:00

  • Transcriptome profiling and molecular marker discovery in red pepper, Capsicum annuum L. TF68.

    abstract::Transcriptome from high throughput sequencing-by-synthesis is a good resource of molecular markers. In this study, we present utility of massively parallel sequencing by synthesis for profiling the transcriptome of red pepper (Capsicum annuum L. TF68) using 454 GS-FLX pyrosequencing. Through the generation of approxim...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1102-x

    authors: Lu FH,Cho MC,Park YJ

    更新日期:2012-03-01 00:00:00

  • Phosphorylation of proteasomes in mammalian cells.

    abstract::20S proteasomes are large (700 kDa) proteinase complexes which form the central catalytic core of larger complexes (26S proteasomes or PA28-20S complexes) formed by association with regulatory particles. These larger complexes are involved in diverse regulatory processes in the cell including cyclin breakdown, proteol...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006969517958

    authors: Bose S,Mason GG,Rivett AJ

    更新日期:1999-04-01 00:00:00

  • The expression of analgesic-antitumor peptide (AGAP) from Chinese Buthus martensii Karsch in transgenic tobacco and tomato.

    abstract::The present study aimed to obtain analgesic-antitumor peptide (AGAP) gene expression in plants. The analgesic-antitumor peptide (AGAP) gene was from the venom of Buthus martensii Karsch. Previous studies showed that AGAP has both analgesic and antitumor activities, suggesting that AGAP would be useful in clinical situ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-008-9277-5

    authors: Lai L,Huang T,Wang Y,Liu Y,Zhang J,Song Y

    更新日期:2009-05-01 00:00:00

  • Affinity purification of histidine-tagged proteins.

    abstract::Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low ex...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF01674434

    authors: Schmitt J,Hess H,Stunnenberg HG

    更新日期:1993-10-01 00:00:00

  • Structure and functional analysis of the 26S proteasome subunits from plants.

    abstract::As initial steps to define how the 26S proteasome degrades ubiquitinated proteins in plants, we have characterized many of the subunits that comprise the proteolytic complex from Arabidopsis thaliana. A set of 23 Arabidopsis genes encoding the full complement of core particle (CP) subunits and a collection encoding 12...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006926322501

    authors: Fu H,Girod PA,Doelling JH,van Nocker S,Hochstrasser M,Finley D,Vierstra RD

    更新日期:1999-04-01 00:00:00

  • Lipoprotein-associated phospholipase A2 gene V279F polymorphisms and coronary heart disease: a meta-analysis.

    abstract::Lipoprotein-associated phospholipase A2 (LP-PLA2) may play an important role in the pathophysiology of coronary heart disease (CHD). The polymorphism of LP-PLA2 gene caused LP-PLA2 enzyme activity depressing or lost. But there is not a definite conclusion for the association of between the LP-PLA2 gene polymorphism an...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-010-0529-9

    authors: Zheng GH,Chen HY,Xiong SQ,Chu JF

    更新日期:2011-08-01 00:00:00

  • Developmental and photosynthetic regulation of δ-endotoxin reveals that engineered sugarcane conferring resistance to 'dead heart' contains no toxins in cane juice.

    abstract::The phosphoenolpyruvate (PEP) carboxylase is regulated at the levels of transcription and post-translation in C4 plants in light and abundantly accumulates in leaf mesophyll cells. We report here developmental and photosynthetic regulation of stably accumulated Bacillus thuringiensis δ-endotoxin under the control of P...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0369-7

    authors: Khan MS,Ali S,Iqbal J

    更新日期:2011-04-01 00:00:00

  • Green cocoons in silkworm Bombyx mori resulting from the quercetin 5-O-glucosyltransferase of UGT86, is an evolved response to dietary toxins.

    abstract::The glycosylation of UDP-glucosyltransferases (UGTs) is of great importance in the control and elimination of both endogenous and exogenous toxins. Bm-UGT10286 (UGT86) is the sole provider of UGT activity against the 5-O position of quercetin and directly influences the formation of green pigment in the Bombyx cocoon....

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2437-7

    authors: Xu X,Wang M,Wang Y,Sima Y,Zhang D,Li J,Yin W,Xu S

    更新日期:2013-05-01 00:00:00

  • Association between the CTLA-4 +49 A/G polymorphism and susceptibility to rheumatoid arthritis: a meta-analysis.

    abstract::The aim of this study was to explore whether the cytotoxic T lymphocyte antigen-4 (CTLA-4) +49 A/G polymorphism confers susceptibility to rheumatoid arthritis (RA). A meta-analysis was conducted on the associations between CTLA-4 +49 A/G polymorphism and RA using; 1) allele contrast, 2) the recessive model, 3) the dom...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-011-1364-3

    authors: Lee YH,Bae SC,Choi SJ,Ji JD,Song GG

    更新日期:2012-05-01 00:00:00

  • Carnosic acid prevented olanzapine-induced metabolic disorders through AMPK activation.

    abstract::Olanzapine, an atypical antipsychotic medication, has been associated with weight gain and metabolic toxicity, especially in long term usage. Carnosic acid (CA), a major constituent of rosemary extract, has been shown to improve metabolic abnormalities. In this experiment, the effect of CA on olanzapine-induced obesit...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05825-5

    authors: Razavi BM,Abazari AR,Rameshrad M,Hosseinzadeh H

    更新日期:2020-10-01 00:00:00

  • Alterations of cell cycle genes in cancer: unmasking the role of cancer stem cells.

    abstract::The cell cycle is a complex and strictly controlled process, consisting of different phases. Cell cycle regulation depends on phase-specific transcriptions of cell cycle genes. The alterations of cell cycle genes can predispose normal cells to have a cancerous phenotype. Indeed, several mechanisms underlying the dereg...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1007/s11033-020-05341-6

    authors: Caglar HO,Biray Avci C

    更新日期:2020-04-01 00:00:00

  • Macromolecular synthesis in mycobacteriophage I3 infected cells.

    abstract::DNA-, RNA- and protein synthesis have been studied in Mycobacterium smegmatis cells infected with phage I3. The macromolecular synthesis continued until the end of latent period. Early RNA and protein synthesis were necessary prior to the commencement of DNA replication. The infecting phage DNA sedimented as larger th...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00798378

    authors: Nagaraja V,Gopinathan KP

    更新日期:1981-11-30 00:00:00

  • Overexpressed RACK1 is positively correlated with malignant degree of human colorectal carcinoma.

    abstract::RACK1 is a crucial scaffold and anchoring protein, which plays a vital role in multiple signaling pathways of tumorigenesis. The aim of the present study was to identify the correlation between expressions of RACK1 and malignant degrees in colorectal carcinoma (CRC) patients. All together 157 CRC patients were enrolle...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3201-y

    authors: Jin S,Mu Y,Wang X,Liu Z,Wan L,Xiong Y,Zhang Y,Zhou L,Li L

    更新日期:2014-05-01 00:00:00

  • Novel single nucleotide polymorphisms of bovine SREBP1 gene is association with fatty acid composition and marbling score in commercial Korean cattle (Hanwoo).

    abstract::The sterol regulatory element binding factor 1 (SREBP1) gene plays an important role in the biosynthesis of fatty acids. The aim of this study was to investigate the association between SNPs within SREBP1 gene and fatty acid composition of muscle fat and marbling score in a commercial population of Korean cattle. Amon...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2055-4

    authors: Lee Y,Oh D,Lee J,La B,Yeo J

    更新日期:2013-01-01 00:00:00

  • Gene expression and pathologic response to neoadjuvant chemotherapy in breast cancer.

    abstract::Pathologic complete response after neoadjuvant systemic treatment appears to be a valid surrogate for better overall survival in breast cancer patients. Currently, together with standard clinicopathologic assessment, novel molecular biomarkers are being exhaustively tested in order to look into the heterogeneity of br...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-1576-1

    authors: Kolacinska A,Fendler W,Szemraj J,Szymanska B,Borowska-Garganisz E,Nowik M,Chalubinska J,Kubiak R,Pawlowska Z,Blasinska-Morawiec M,Potemski P,Jeziorski A,Morawiec Z

    更新日期:2012-07-01 00:00:00

  • Selection of reference genes for quantitative real time RT-PCR during dimorphism in the zygomycete Mucor circinelloides.

    abstract::Mucor circinelloides is a dimorphic fungal model for studying several biological processes including cell differentiation (yeast-mold transitions) as well as biodiesel and carotene production. The recent release of the first draft sequence of the M. circinelloides genome, combined with the availability of analytical m...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3818-x

    authors: Valle-Maldonado MI,Jácome-Galarza IE,Gutiérrez-Corona F,Ramírez-Díaz MI,Campos-García J,Meza-Carmen V

    更新日期:2015-03-01 00:00:00

  • Methylation status and protein expression of RASSF1A in breast cancer patients.

    abstract::Recently genetics and epigenetics alterations have been found to be characteristic of malignancy and hence can be used as targets for detection of neoplasia. RAS association domain family protein 1A (RASSF1A) gene hypermethylation has been a subject of interest in recent researches on cancer breast patients. The aim o...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2837-3

    authors: Hagrass HA,Pasha HF,Shaheen MA,Abdel Bary EH,Kassem R

    更新日期:2014-01-01 00:00:00