Localization of small RNAs hybridizable to a B2 clone in the nuclear fraction of mouse cell lines.

abstract：:We have cloned two full-length cDNAs from two ferritin genes (Aifer1 and Aifer2) of the bay scallop, Argopecten irradians (Lamarck 1819). The cDNAs are 1,019 and 827 bp in length and encode proteins of 171 and 173 amino acids, respectively. The 5' UTR of each contains a conserved iron response element (IRE) motif. Seq...

journal_title：Molecular biology reports

authors： He X,Zhang Y,Wu X,Xiao S,Yu Z

更新日期：2011-03-01 00:00:00

abstract：:Complementary DNA to mouse globin mRNA was prepared using viral reverse transcriptase. Molecular hybridisation of this cDNA to Friend virus RNA demonstrates the absence of globin gene sequences. ...

journal_title：Molecular biology reports

authors： Pragnell IB,Gilmour RS

更新日期：1973-12-01 00:00:00

abstract：:Spata34 is a testis-specific-expressed gene which exerts diverse functions in testis development. This study intends to examine the expression profiles of Spata34 in postnatal rat testis, and explore its potential roles in cell proliferation in vitro. We found that the mRNA and protein expression levels of Spata34 wer...

journal_title：Molecular biology reports

authors： Nie D,Li H,Liu Y,Liao Z

更新日期：2018-12-01 00:00:00

abstract：:Overexpression of domains of a human protein using recombinant DNA technology has been challenging because individual domains intend to accumulate as non-soluble aggregate when expressed separately. Studies on identifying right sequences for a domain to be able to fold independently may help understand the folding pat...

journal_title：Molecular biology reports

authors： Tiwary M,Agarwal N,Dinda A,Yadav SC

更新日期：2016-05-01 00:00:00

abstract：:Brassica oleracea var. capitata (cabbage) is an economically important crop affected by black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). MicroRNAs (miRNAs) play an important role in plant defense modulation and therefore the analysis of these molecules can help better understand plant-pathogen ...

journal_title：Molecular biology reports

authors： Santos LS,Maximiano MR,Megias E,Pappas M,Ribeiro SG,Mehta A

更新日期：2019-06-01 00:00:00

abstract：:CD40 signaling plays a critical role in the survival rate of gastric cancer patients. Tumour samples were collected from 73 patients with who were diagnosed as gastric cancer in general surgery department in the 1st affiliated hospital of Suzhou University between September 2002 and July 2003. All patients had not rec...

journal_title：Molecular biology reports

authors： Li R,Chen WC,Pang XQ,Tian WY,Wang WP,Zhang XG

更新日期：2012-09-01 00:00:00

abstract：:The immunity and health status of ornamental fish is an important aspect, as they are kept in a confined environment and various stressful conditions which lead to depletion of overall colourful appearance and mortality. The carotenoids can act as immunity boosters in captive aquarium system and may be supplemented in...

journal_title：Molecular biology reports

authors： Tripathy PS,Khatei A,Parhi J,Choudhury TG,Sahoo L,Mandal SC

更新日期：2019-12-01 00:00:00

abstract：:The cell divisions cycle 42 (Cdc42) gene has been characterized in the fungi, such as Candida albicans, Penicillium marneffei, and Wangiella (Exophiala) dermatitidis, which plays important roles during growth and development. The partial cDNA sequence of Cdc42 of Fonsecaea monophora (F. monophora), designated FmCdc42,...

journal_title：Molecular biology reports

authors： Xie Z,Feng P,Zhang J,Li X,Sun J,Lu C,Huang H,Xi L

更新日期：2012-02-01 00:00:00

abstract：:Imprinted genes play important roles in mammalian growth, development and behavior. In this study, we obtained 1568 bp mRNA sequence of porcine DIO3 (deiodinase, iodothyronine, type III), and also identified its imprinting status during porcine fetal development. The complete open reading frame (ORF) encoding 278 amin...

journal_title：Molecular biology reports

authors： Qiao M,Wu HY,Guo L,Mei SQ,Zhang PP,Li FE,Zheng R,Deng CY

更新日期：2012-03-01 00:00:00

abstract：:Tumor angiogenesis, a major requirement for tumor growth and metastasis, is regulated by pro- and anti-angiogenic factors. The aim of this study was to quantify the expression of angiogenic (VEGF, HIF-1α, Angiopiotein-2) and anti-angiogenic (endostatin, angiostatin and Thrombospondin-1) factors and to discern their cl...

journal_title：Molecular biology reports

authors： Sharma BK,Srinivasan R,Kapil S,Singla B,Chawla YK,Chakraborti A,Saini N,Duseja A,Das A,Kalra N,Dhiman RK

更新日期：2013-10-01 00:00:00

abstract：:MADS-box genes encode important transcription factors in plants that are involved in many processes during plant growth and development. An investigation of the soybean genome revealed 106 putative MADS-box genes. These genes were classified into two classes, type I and type II, based on phylogenetic analysis. The soy...

journal_title：Molecular biology reports

authors： Shu Y,Yu D,Wang D,Guo D,Guo C

更新日期：2013-06-01 00:00:00

abstract：:The programmed cell death (PCD) is important in maintaining the cell number homoeostasis of tissues and organs in metazoan. This process is regulated by the inhibitors of apoptosis proteins (IAPs). The function and mechanism of IAPs have been well studied in animal embryonic development and human cancers. However, the...

journal_title：Molecular biology reports

authors： He HJ,Hou L,Wang JX,Zhao XF

更新日期：2012-02-01 00:00:00

abstract：:Beta(2)-microglobulin (beta(2)m), a protein necessary for proper folding, peptide binding, and surface display of class I antigens plays an important role in immune response. The full-length cDNA containing beta(2)m was cloned from the spleen cDNA library of large yellow croaker Pseudosciaena crocea (Pscr-beta ( 2 ) m...

journal_title：Molecular biology reports

authors： Yu S,Chen X,Ao J

更新日期：2009-09-01 00:00:00

abstract：:A nucleic acid sequence MC, encoding Momordica Chanrantia anti-hyperglycaemic peptide MC6 (accession: AAX06814) synthesized according to Escherichia coli preferred codons, was cloned and expressed in E. coli. Recombinant protein pQE8-MC (about 3.5 kDa) was purified and analyzed by 20% SDS-PAGE and western blot. It rev...

journal_title：Molecular biology reports

authors： Liu SX,Fu ZP,Mu RM,Hu ZB,Wang FJ,Wang XR

更新日期：2010-04-01 00:00:00

abstract：:Sheep are valuable resources for the wool industry. Wool growth of Aohan fine wool sheep has cycled during different seasons in 1 year. Therefore, identifying genes that control wool growth cycling might lead to ways for improving the quality and yield of fine wool. In this study, we employed Agilent sheep gene expres...

journal_title：Molecular biology reports

authors： Liu N,Li H,Liu K,Yu J,Cheng M,De W,Liu J,Shi S,He Y,Zhao J

更新日期：2014-08-01 00:00:00

abstract：:Transgenic doubled haploid rapeseed (Brassica napus L. cvs. Global and PF(704)) plants were obtained from microspore-derived embryo (MDE) hypocotyls using the microprojectile bombardment. The binary vector pCAMBIA3301 containing the gus and bar genes under control of CaMV 35S promoter was used for bombardment experime...

journal_title：Molecular biology reports

authors： Abdollahi MR,Moieni A,Mousavi A,Salmanian AH

更新日期：2011-02-01 00:00:00

abstract：:The spleen plays a crucial role in innate and adaptive immunity in bony fish. Consequently, this organ is well suited to assess the immune competence of an organism and hence provides useful information for comparison and classification of production traits of food fish, such as robustness and susceptibility. To gain ...

journal_title：Molecular biology reports

authors： Köbis JM,Rebl A,Kühn C,Goldammer T

更新日期：2013-02-01 00:00:00

abstract：:Various investigations have been so far performed for extraction of genomic DNA from plant tissues, in which the extracted intact DNA can be exploited for a diverse range of biological studies. Extraction of high quality DNA from leathery plant tissues (e.g., coniferous organs) appears to be a critical stage. Moreover...

journal_title：Molecular biology reports

authors： Barzegari A,Vahed SZ,Atashpaz S,Khani S,Omidi Y

更新日期：2010-02-01 00:00:00

abstract：:In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via t...

journal_title：Molecular biology reports

authors： Meng Q,Xiao Z,Yuan H,Xue F,Zhu Y,Zhou X,Yang B,Sun J,Meng B,Sun X,Cheng F

更新日期：2012-02-01 00:00:00

abstract：:Protein phosphatases type 2C (PP2Cs) from group A, which includes the ABI1/HAB1 and PP2CA branches, are key negative regulators of ABA signaling. HAI-1 gene had been shown to affect both seed and vegetative responses to ABA, which is one of PP2Cs clade A in Arabidopsis thaliana. Transgenic plants containing pHAI-1::GU...

journal_title：Molecular biology reports

authors： Zhang J,Li X,He Z,Zhao X,Wang Q,Zhou B,Yu D,Huang X,Tang D,Guo X,Liu X

更新日期：2013-03-01 00:00:00

abstract：:Disorders in the proliferation and apoptosis of thyrocytes may induce goitre, adenoma and carcinoma in the thyroid. The Wnt/beta-catenin pathway has been demonstrated to be involved in the regulation of cell proliferation, differentiation and apoptosis in various cell lines. The regulatory mechanism on the proliferati...

journal_title：Molecular biology reports

authors： Chen G,Jiang Q,You Z,Yao J,Mou L,Lin X,Shen X,You T,Lin Q,Wen J,Lin L

更新日期：2010-07-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a newly identified class of non-coding small RNAs of about 21-24 nucleotides. They play important roles in multiple biological processes by degrading targeted mRNAs or repressing mRNA translation. To date, a total of 2,043 plant miRNAs are present in the miRNA Registry database (miRBase Release ...

journal_title：Molecular biology reports

authors： Lang Q,Jin C,Lai L,Feng J,Chen S,Chen J

更新日期：2011-03-01 00:00:00

abstract：:Mitochondria are highly specialized in function, but mitochondrial and, therefore, cellular integrity is maintained through their dynamic nature. Through the frequent processes of fusion and fission, mitochondria continuously change in shape and adjust function to meet cellular requirements. Abnormalities in fusion/fi...

journal_title：Molecular biology reports

authors： Chen L,Winger AJ,Knowlton AA

更新日期：2014-11-01 00:00:00

abstract：:Haemoglobin meseenger RNA was isolated from spleen erythroïd cells of anaemic rabbits. The mRNA fraction looks quite homogeneous when analyzed by sucrose gradient centrifugation; it sediments in the 9S region. When added to an ascites cell free system, spleen 9S RNA stimulates the incorporation of radioactive leucine ...

journal_title：Molecular biology reports

authors： Nokin P,Gautier E

更新日期：1973-06-01 00:00:00

abstract：:Testis development is a highly regulated sequence of developmental process that spans from the establishment of germ cell lineage during embryonic development to the periodic wave of spermatogenesis in adulthood. The normal development of testes and the fertility of male animals require specific cell types to respond ...

journal_title：Molecular biology reports

authors： Fan YS,Hu YJ,Yang WX

更新日期：2012-04-01 00:00:00

abstract：:Evidence indicates that both S-adenosylmethionine (SAMe) metabolism and intramuscular fat are associated with insulin resistance and type II diabetes. However, it is still unknown whether SAMe have effects on intramuscular adipogenesis. The present study investigated the roles of SAMe in the adipogenic differentiation...

journal_title：Molecular biology reports

authors： Yue T,Fang Q,Yin J,Li D,Li W

更新日期：2010-10-01 00:00:00

abstract：:The relationship between genetic polymorphisms of glutathione S-transferase (GST) and the development of glioma has been investigated in several epidemiologic studies. However these studies report inconsistent results. In order to quantitatively summarise the evidence for such a relationship, a meta-analysis is conduc...

journal_title：Molecular biology reports

authors： Fan Z,Wu Y,Shen J,Zhan R

更新日期：2013-02-01 00:00:00

abstract：:Autoantibodies to SS-A/Ro are among the most common found in sera of patients with systemic rheumatic diseases. These autoimmune diseases can affect various organ systems of the body and are variable in their manifestations and presentation. One of the autoimmune targets is the 60 kDa SS-A/Ro protein known to be assoc...

journal_title：Molecular biology reports

authors： Wang D,Buyon JP,Chan EK

更新日期：1996-01-01 00:00:00

abstract：:microRNAs (miRNAs) are a new class of small non-coding RNAs that function as tumor suppressors or oncogenes. Single nucleotide polymorphisms (SNPs) in miRNA may contribute to cancer development. We hypothesized that genetic variations of the miRNA could be associated with the risk of hepatitis B virus (HBV)-related he...

journal_title：Molecular biology reports

authors： Xiang Y,Fan S,Cao J,Huang S,Zhang LP

更新日期：2012-06-01 00:00:00

abstract：:The association between vascular endothelial growth factor (VEGF) gene polymorphisms and risk of head and neck cancer (HNC) were investigated in many published studies; however, the currently available results are inconclusive. Therefore, we conducted this meta-analysis for deriving a more precise estimation of associ...

journal_title：Molecular biology reports

authors： Leng WD,He MN,Chen QL,Gong H,Zhang L,Zeng XT

更新日期：2013-10-01 00:00:00