当前位置： SCI文献检索 > MOLECULAR BIOLOGY REPORTS期刊下所有文献 > Expression patterns of As-ClC gene of Artemia sinica in early development and under salinity stress.

Expression patterns of As-ClC gene of Artemia sinica in early development and under salinity stress.

Abstract：

:As-ClC (chloride channels protein from Artemia sinica), a member from the chloride channels protein family, is a α-helical membrane protein predicted to traverse the cell membrane 11 times. It is important for several physiological functions such as cell volume regulation, cell proliferation, growth and differentiation. In this paper, the complete cDNA sequence of As-CIC was cloned from A. sinica for the first time using RACE technology. The expression pattern and location of the As-CIC gene was investigated in different stages of the embryonic development by means of quantitative real-time PCR and in situ hybridization (ISH) assay. As-CLC was distributed throughout the whole body in cells of different embryonic development of A. sinica as shown by ISH. There was a low expression level of the As-ClC gene after 0 h and a higher expression level after 15 and 40 h when the embryo entered the next growth period and the environmental salinity changed. At adult stage, the As-ClC maintained a high expression level. The results of the real-time PCR assay showed an increasing trend of As-ClC transcripts with increasing salinity. The expression of As-ClC was higher in the control group (28) than in the experimental group except at a salinity of 200 PSU. It indicated that As-ClC functions as salinity-stress-related gene, probably participated in cell volume regulation and osmotic regulation during the early embryonic development of A. sinica.

journal_name

Mol Biol Rep

journal_title

Molecular biology reports

authors

Zhang Q,Hou M,Li Q,Han L,Yuan Z,Tan J,Du B,Zou X,Hou L

doi

10.1007/s11033-012-2441-y

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

3655-64

issue

5

eissn

0301-4851

issn

1573-4978

journal_volume

40

pub_type

杂志文章

相关文献

MOLECULAR BIOLOGY REPORTS文献大全
  • A highly sensitive immuno-PCR assay for detection of H5N1 avian influenza virus.

    abstract：:With an aim at detecting the ultra-low concentration of avian influenza virus (AIV), a highly sensitive hybrid assay based on immunology and polymerase chain reaction was developed. The TopYield microtiter plates were coated with ten-fold serial dilutions of H5N1 subtype AIV ranging from 10 EID(50 )ml(-1)~10(-4) EID(5...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0315-8

    authors： Deng MJ,Xiao XZ,Zhang YM,Wu XH,Zhu LH,Xin XQ,Wu DL

    更新日期：2011-03-01 00:00:00

  • Differentially expressed three non-coding alternate exons at 5' UTR of regulatory type I beta subunit gene of mouse.

    abstract：:Prkar1b gene encodes regulatory type I, beta subunit (RIβ) of cAMP dependent protein kinase A in mouse. Among the various isoforms of regulatory and catalytic subunits that comprise mammalian PKA, RIβ subunit is considered to be one of the important subunits for neuronal functions. This is involved in multiple forms o...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-1108-4

    authors： Banday AR,Azim S,Tabish M

    更新日期：2012-04-01 00:00:00

  • TGF-β superfamily: how does it regulate testis development.

    abstract：:Testis development is a highly regulated sequence of developmental process that spans from the establishment of germ cell lineage during embryonic development to the periodic wave of spermatogenesis in adulthood. The normal development of testes and the fertility of male animals require specific cell types to respond ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,评审

    doi：10.1007/s11033-011-1265-5

    authors： Fan YS,Hu YJ,Yang WX

    更新日期：2012-04-01 00:00:00

  • Cloning and characterization of a novel stress-responsive WRKY transcription factor gene (MusaWRKY71) from Musa spp. cv. Karibale Monthan (ABB group) using transformed banana cells.

    abstract：:WRKY transcription factor proteins play significant roles in plant stress responses. Here, we report the cloning and characterization of a novel WRKY gene, MusaWRKY71 isolated from an edible banana cultivar Musa spp. Karibale Monthan (ABB group). MusaWRKY71, initially identified using in silico approaches from an abio...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0521-4

    authors： Shekhawat UK,Ganapathi TR,Srinivas L

    更新日期：2011-08-01 00:00:00

  • Influence of highly curved DNA segments on in vivo topology of plasmids.

    abstract：:Recombinant plasmids carrying a highly curved DNA structure are sometimes unstable in Escherichia coli. In order to know the underlying mechanism, several plasmids carrying one or two highly bent DNA segment(s) from the human adenovirus type 2 (Ad2) enhancer and/or origin region of phage lambda replication were system...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1023/a:1007017121313

    authors： Ohyama T,Miyano M,Sakuma S

    更新日期：1999-12-01 00:00:00

  • Isolation of a DNA-protein complex containing a single DNA fragment which is at or near the replication origin of the Bacillus subtilis chromosome.

    abstract：:A chromosomal fragment containing purA, the nearest marker from the replication origin of the Bacillus subtilis chromosome, was highly purified as a complex containing at least proteins and being solubilized easily during cell lysis. The complex had a markedly higher sedimentation rate (70-120S) than the bulk of the s...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00356932

    authors： Yamaguchi K,Yoshikawa H

    更新日期：1976-07-01 00:00:00

  • High-throughput retrotransposon-based genetic diversity of maize germplasm assessment and analysis.

    abstract：:Maize is one of the world's most important crops and a model for grass genome research. Long terminal repeat (LTR) retrotransposons comprise most of the maize genome; their ability to produce new copies makes them efficient high-throughput genetic markers. Inter-retrotransposon-amplified polymorphisms (IRAPs) were use...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-020-05246-4

    authors： Ghonaim M,Kalendar R,Barakat H,Elsherif N,Ashry N,Schulman AH

    更新日期：2020-03-01 00:00:00

  • Biochemical and molecular characterization of mitochondrial membrane-bound arginase in Heteropneustes fossilis.

    abstract：:The two predominant forms of arginase, cytosolic Arginase-I and mitochondrial Arginase-II, catalyze hydrolysis of arginine into ornithine and urea. Based on presence of arginase activity in extracts using potassium chloride (KCl), mitochondrial membrane-bound arginase has also been suggested. However, the activity of ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-016-3965-3

    authors： Mishra S,Mishra R

    更新日期：2016-05-01 00:00:00

  • Complete sequence and gene organization of the mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis).

    abstract：:The complete sequence of mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis) is determined by using PCR amplification, clone and primer-walking sequencing methods. The genome is 16836 bp in size, containing 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mtDNA genome of Siamensis Crocodile...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-007-9062-x

    authors： Ji X,Wu X,Yan P,Amato G

    更新日期：2008-06-01 00:00:00

  • A 5'UTR SNP of GHRHR locus is associated with body weight and average daily gain in Chinese cattle.

    abstract：:Growth hormone-releasing hormone receptor (GHRHR) has important functions in the regulation of the growth hormone axis and the development and proliferation of pituitary somatotropes. Moreover, some mutations in mouse GHRHR can induce the dwarfism. The objective of this paper is to reveal the association of GHRHR with...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1927-y

    authors： Zhang CF,Chen H,Zhang ZY,Zhang LZ,Yang DY,Qu YJ,Hua LS,Zhang B,Hu SR

    更新日期：2012-12-01 00:00:00

  • CD40 signal expression in gastric cancer tissue and its correlation with prognosis of gastric cancer patients.

    abstract：:CD40 signaling plays a critical role in the survival rate of gastric cancer patients. Tumour samples were collected from 73 patients with who were diagnosed as gastric cancer in general surgery department in the 1st affiliated hospital of Suzhou University between September 2002 and July 2003. All patients had not rec...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1734-5

    authors： Li R,Chen WC,Pang XQ,Tian WY,Wang WP,Zhang XG

    更新日期：2012-09-01 00:00:00

  • Study of polymorphisms in the promoter region of ovine β-lactoglobulin gene and phylogenetic analysis among the Valle del Belice breed and other sheep breeds considered as ancestors.

    abstract：:The aim of this work was to sequence the promoter region of β-lactoglobulin (BLG) gene in four sheep breeds, in order to identify polymorphisms, infer and analyze haplotypes, and phylogenetic relationship among the Valle del Belice breed and the other three breeds considered as ancestors. Sequencing analysis and align...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-0794-2

    authors： Mastrangelo S,Sardina MT,Riggio V,Portolano B

    更新日期：2012-01-01 00:00:00

  • Identification, characterization and mapping of differentially expressed genes in a winter wheat cultivar (Centenaire) resistant to Fusarium graminearum infection.

    abstract：:Fusarium head blight (FHB), predominantly caused by Fusarium graminearum, is a destructive disease that poses a serious threat to wheat (Triticum aestivum L.) production around the world. A suppression subtractive hybridization (SSH) cDNA library was constructed from F. graminearum infected spikes of a resistant Belgi...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1823-5

    authors： Muhovski Y,Batoko H,Jacquemin JM

    更新日期：2012-10-01 00:00:00

  • Common genetic variation in CYP1B1 is associated with concentrations of T₄, FT₃ and FT₄ in the sera of polycystic ovary syndrome patients.

    abstract：:CYP1B1 encodes an estrogen enzyme that oxidizes 17β-estradiol to 4-hydroxyestradiol. The evidence demonstrates there may be a relationship between CYP1B1 and thyroid function. To date, no study has evaluated if genetic polymorphisms that regulate concentrations of serum FT3 and FT4 contribute to Polycyctic Ovary Syndr...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-2406-1

    authors： Zou S,Sang Q,Wang H,Feng R,Li Q,Zhao X,Xing Q,Jin L,He L,Wang L

    更新日期：2013-04-01 00:00:00

  • Protein profiling analysis of skeletal muscle of a pufferfish, Takifugu rubripes.

    abstract：:Protein profile of the skeletal muscle of Takifugu rubripes, a kind of pufferfish, was carried out with two-dimensional polyacrylamide gel electrophoresis (2-DE). Among the 112 protein spots detected in a silver-stained 2-D polyacrylamide gel, 33 were analyzed by Matrix Assisted Laser Desorption Ionisation tandem time...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-009-9684-2

    authors： Lu J,Zheng J,Liu H,Li J,Chen H,Chen K

    更新日期：2010-06-01 00:00:00

  • Molecular cloning and functional expression analysis of a new gene encoding geranylgeranyl diphosphate synthase from hazel (Corylus avellana L. Gasaway).

    abstract：:Geranylgeranyl diphosphate synthase (GGPPS) [EC 2.5.1.29] catalyzes the biosynthesis of geranylgeranyl diphosphate (GGPP), which is a key precursor for diterpenes such as taxol. Herein, a full-length cDNA encoding GGPPS (designated as CgGGPPS) was cloned and characterized from hazel (Corylus avellana L. Gasaway), a ta...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-009-9935-2

    authors： Wang Y,Miao Z,Tang K

    更新日期：2010-10-01 00:00:00

  • Identification, characterization, and expression analysis of a serotonin receptor involved in the reproductive process of the Pacific abalone, Haliotis discus hannai.

    abstract：:Serotonin receptor (5-HT) is a biogenic amine acting as a neurotransmitter and neuromodulator that mediates various aspects of reproduction and gametogenesis. The full-length nucleotide sequence of Haliotis discus hannai encodes a protein of 417 amino acids with a predicted molecular mass of 46.54 kDa and isoelectric ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-05162-2

    authors： Sharker MR,Sukhan ZP,Kim SC,Lee WK,Kho KH

    更新日期：2020-01-01 00:00:00

  • Association between SHBG Asp327Asn (rs6259) polymorphism and breast cancer risk: a meta-analysis of 10,454 cases and 13,111 controls.

    abstract：:Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that plays an important role in breast cancer pathophysiology and risk definition, since it regulates the bioavailable fraction of circulating estradiol. Epidemiological studies have evaluated the association between SHBG Asp327Asn polymorphism and breast ca...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,meta分析

    doi：10.1007/s11033-012-1680-2

    authors： Zhou JY,Shi R,Yu HL,Zheng WL,Ma WL

    更新日期：2012-08-01 00:00:00

  • Green fluorescent protein is superior to blue fluorescent protein as a quantitative reporter of promoter activity in E. coli.

    abstract：:Fluorescent proteins related to and derived from green fluorescent protein (GFP) are widely used as tools for investigating a wide range of biological processes. In particular, GFP and its relatives have been used extensively as qualitative reporters of gene expression in many different organisms, but relatively few s...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-008-9285-5

    authors： Lissemore JL,Bayes J,Calvey M,Reineke L,Colagiavanni A,Tscheiner M,Mascotti DP

    更新日期：2009-05-01 00:00:00

  • Recent research progress of microRNAs in hypertension pathogenesis, with a focus on the roles of miRNAs in pulmonary arterial hypertension.

    abstract：:Hypertension is the most widespread disease in the world affecting humans and imparts a significant cardiovascular and renal risk to patients, and extensive research over the past few decades has enhanced our understanding of the underlying pathogenesis of hypertension. A growing number of studies have shown that miRN...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,评审

    doi：10.1007/s11033-018-4335-0

    authors： Miao C,Chang J,Zhang G

    更新日期：2018-12-01 00:00:00

  • Improvement in cyst recovery and molecular detection of Giardia duodenalis from stool samples.

    abstract：:Molecular detection of Giardia duodenalis by polymerase chain reaction (PCR) is difficult in faecal samples due to inhibitors that contaminate DNA preparations, or due to low cyst concentrations. In order to eliminate inhibitors, improve cyst recovery and molecular detection of G. duodenalis, different types of water,...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-05224-5

    authors： Bezagio RC,Colli CM,Romera LIL,de Almeida CR,Ferreira ÉC,Mattia S,Gomes ML

    更新日期：2020-02-01 00:00:00

  • Kinetic and thermodynamic study of cloned thermostable endo-1,4-β-xylanase from Thermotoga petrophila in mesophilic host.

    abstract：:The 1,044 bp endo-1,4-β-xylanase gene of a hyperthermophilic Eubacterium, "Thermotoga petrophila RKU 1" (T. petrophila) was amplified, from the genomic DNA of donor bacterium, cloned and expressed in mesophilic host E. coli strain BL21 Codon plus. The extracellular target protein was purified by heat treatment followe...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1555-6

    authors： ul Haq I,Hussain Z,Khan MA,Muneer B,Afzal S,Majeed S,Akram F

    更新日期：2012-07-01 00:00:00

  • Sequence variation in promoter regions of genes for CC chemokine ligands (CCL)19 and 21 in Czech patients with myocardial infarction.

    abstract：:Recruitment of inflammatory cells to the arterial wall is an important pathogenic mechanism of atherosclerosis and coronary artery disease (CAD). Functional variability in the genes encoding for chemokines that promote infiltration of atherosclerotic plaques by macrophages and lymphocytes may therefore contribute to t...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3175-9

    authors： Stahelova A,Petrkova J,Petrek M,Mrazek F

    更新日期：2014-05-01 00:00:00

  • Cucumber mosaic virus RNA contains 7-methyl guanosine at the 5'-terminus of all four RNA species.

    abstract：:Purified cucumber mosaic virus (CMV) RNA was reacted with NaIO4 and then [3H]KBH4 to label ends containing exposed 2',3'-hydroxyls. The four major RNA species were then fractionated by polyacrylamide gel electrophoresis. Analysis of digests of these RNAs by ribonucleases T1 plus T2, alkaline phosphatease, snake venom ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00357014

    authors： Symons RH

    更新日期：1975-12-01 00:00:00

  • Mesenchymal stem cell-based therapy for autoimmune diseases: emerging roles of extracellular vesicles.

    abstract：:In autoimmune disease body's own immune system knows healthy cells as undesired and foreign cells. Over 80 types of autoimmune diseases have been recognized. Currently, at clinical practice, treatment strategies for autoimmune disorders are based on relieving symptoms and preventing difficulties. In other words, there...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,评审

    doi：10.1007/s11033-019-04588-y

    authors： Rad F,Ghorbani M,Mohammadi Roushandeh A,Habibi Roudkenar M

    更新日期：2019-02-01 00:00:00

  • Combination treatment with dendrosomal nanocurcumin and doxorubicin improves anticancer effects on breast cancer cells through modulating CXCR4/NF-κB/Smo regulatory network.

    abstract：:Despite advantageous antitumor properties of doxorubicin, the considerable cytotoxicity of this chemotherapeutic agent has made it necessary to develop combination treatment strategies. The aim of the current study was to investigate the possible synergism between dendrosomal nanocurcumin (DNC) and doxorubicin in elic...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-017-4115-2

    authors： Mahjoub MA,Bakhshinejad B,Sadeghizadeh M,Babashah S

    更新日期：2017-08-01 00:00:00

  • Isolation and characterization of a gene encoding an ethylene responsive factor protein from Ceratoides arborescens.

    abstract：:Ethylene responsive factor (ERF) proteins play important roles in plant growth and development and regulate biotic and abiotic stress responses. In this study, a full length mRNA encoding a novel ERF-type transcription factor namely Ceratoides arborescens ERF protein (CeERF) was isolated from C. arborescens. The deduc...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-0869-0

    authors： Dong J,Wang X,Wang K,Wang Z,Gao H

    更新日期：2012-02-01 00:00:00

  • Human intracerebroventricular (ICV) injection of autologous, non-engineered, adipose-derived stromal vascular fraction (ADSVF) for neurodegenerative disorders: results of a 3-year phase 1 study of 113 injections in 31 patients.

    abstract：:We have chosen to test the safety of human intracerebroventricular (ICV) brain injections of autologous non-genetically-modified adipose-derived stromal vascular fraction (ADSVF). In this IRB-approved trial, 24 patients received ICV ADSVF via an implanted reservoir between 5/22/14 and 5/22/17. Seven others were inject...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04983-5

    authors： Duma C,Kopyov O,Kopyov A,Berman M,Lander E,Elam M,Arata M,Weiland D,Cannell R,Caraway C,Berman S,Scord K,Stemler L,Chung K,Khoudari S,McRory R,Duma C,Farmer S,Bravo A,Yassa C,Sanathara A,Singh E,Rapaport B

    更新日期：2019-10-01 00:00:00

  • Development of microsatellite markers using next-generation sequencing for the columnar cactus Echinopsis chiloensis (Cactaceae).

    abstract：:The aim of this study was to develop microsatellite markers as a tool to study population structure, genetic diversity and effective population size of Echinopsis chiloensis, an endemic cactus from arid and semiarid regions of Central Chile. We developed 12 polymorphic microsatellite markers for E. chiloensis using ne...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-016-4069-9

    authors： Ossa CG,Larridon I,Peralta G,Asselman P,Pérez F

    更新日期：2016-12-01 00:00:00

  • S-adenosylmethionine stimulates fatty acid metabolism-linked gene expression in porcine muscle satellite cells.

    abstract：:Evidence indicates that both S-adenosylmethionine (SAMe) metabolism and intramuscular fat are associated with insulin resistance and type II diabetes. However, it is still unknown whether SAMe have effects on intramuscular adipogenesis. The present study investigated the roles of SAMe in the adipogenic differentiation...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-009-9893-8

    authors： Yue T,Fang Q,Yin J,Li D,Li W

    更新日期：2010-10-01 00:00:00