Abstract：
:Recombinant plasmids carrying a highly curved DNA structure are sometimes unstable in Escherichia coli. In order to know the underlying mechanism, several plasmids carrying one or two highly bent DNA segment(s) from the human adenovirus type 2 (Ad2) enhancer and/or origin region of phage lambda replication were systematically constructed and propagated in E. coli. The highly bent DNA segments disturbed the action of DNA topoisomerases: i.e. they were shown to be able to produce an anomalously wide spectrum of linking number topoisomers that tails toward lower supercoiling with a little of the DNA actually positively supercoiled. Furthermore, bent DNA caused multimeric plasmid formation. The linking number topoisomers and multimers seemed to be intermediate topological states of the bent DNA-containing plasmids that would lead to the deletion occurring in them. The nucleotide sequence of a deletion product of a bent DNA-containing plasmid showed that the putative source of the severe topological constraint was entirely eliminated from the plasmid.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Ohyama T,Miyano M,Sakuma Sdoi
10.1023/a:1007017121313keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
269-76issue
4eissn
0301-4851issn
1573-4978journal_volume
26pub_type
杂志文章abstract：:The binding of a cell nucleus stain, hematoxylin (HTL), to bovine serum albumin (BSA) was studied by spectroscopy including fluorescence spectra, UV-Visible absorption, circular dichroism (CD) spectra, synchronous and three-dimensional fluorescence spectra. The results indicated that the binding had led to static fluo...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9448-z
更新日期：2009-11-01 00:00:00
abstract：:Due to being stable in the circulatory system plasma miRNAs have been detected in various diseases including coronary artery disease (CAD) which is the major cause of mortality and morbidity worldwide. Atherosclerosis is the major cause of CAD. The first and most important event in the progression of atherosclerosis i...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4547-3
更新日期：2019-02-01 00:00:00
abstract：:Whole-exome sequencing (WES) is an ideal method for the diagnosis of autosomal recessive diseases. The aim of this study was to evaluate the diagnostic power of WES in patients with autosomal recessive inheritance and to determine the relationship between genotype and phenotype. Retrospective screenings of 24 patients...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4470-7
更新日期：2019-02-01 00:00:00
abstract：:Ribosomes were isolated from baby hamster kidney fibroblasts, either 20 min or 2 days after labelling with radioactive amino acids, and their proteins subjected to two-dimensional polyacrylamide gel electrophoresis. No significant differences were observed between the amounts of radioactivity associated with the posit...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00777524
更新日期：1980-12-31 00:00:00
abstract：:The phenylalanine and the phenylalanyl-tRNAPhe binding sites on the subunits of phenylalanyl-tRNA synthetase from E. coli MRE-600 were localized using p-azidoanilidate of [14C]phenylalanine and N-bromoacetyl-[14C]phenylalanyl-tRNAPhe. The phenylalanine recognizing site was shown to be situated on the alpha subunit of ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00778515
更新日期：1982-03-31 00:00:00
abstract：:The present study was designed to investigate the protective effect of ferulic acid (FA) against isoproterenol (ISO)-induced cardiac toxicity in rats. Isoproterenol challenged in a dose of 85 mg/kg body weight (b.w.) subcutaneously for two consecutive days in the experimental group resulted in acute cardiac toxicity a...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4297-2
更新日期：2018-10-01 00:00:00
abstract：:Lipoprotein-associated phospholipase A2 (LP-PLA2) may play an important role in the pathophysiology of coronary heart disease (CHD). The polymorphism of LP-PLA2 gene caused LP-PLA2 enzyme activity depressing or lost. But there is not a definite conclusion for the association of between the LP-PLA2 gene polymorphism an...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-010-0529-9
更新日期：2011-08-01 00:00:00
abstract：:The present study demonstrates that protein biosynthesis can be studied in single isolated human scalp hair follicles. The matrix and the sheath are the main regions where amino acids are built in. Incorporation is linear for at least five hours. The newly synthesized proteins can be separated into a water-soluble, a ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00777556
更新日期：1979-02-15 00:00:00
abstract：:The aim of this study was to explore candidate single nucleotide polymorphisms (SNPs) and candidate mechanisms of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Two SLE genome-wide association studies (GWASs) datasets were included in this study. Meta-analysis was conducted using 737,984 SNPs in 1,5...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-012-1952-x
更新日期：2012-12-01 00:00:00
abstract：:Free cytoplasmic mRNPs were isolated from human placenta. An activity of RNase was associated with these particles but was mostly inhibited by a labile protein inhibitor. Both RNase and RNase inhibitor were extractable from mRNPs by 0.5 M KCl. The nature of the association of the RNase-RNase inhibitor complex with mRN...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00775355
更新日期：1984-01-01 00:00:00
abstract：:A reduction in the number of functional β-cells is the central pathological event in diabetes. Liver and ventral pancreas differentiates simultaneously in the same general domain of cells within embryonic endoderm. In addition, the precursor cell population being bi-potential may be targeted for either pancreas or liv...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04870-z
更新日期：2019-10-01 00:00:00
abstract：:We tried to study the possible effects of lipoic acid (LA) on adhesion molecule expression and its underlying mechanism in the prevention and treatment of cardiovascular disorders. Intercellular adhesion molecule-1 (ICAM-1) expression and endothelial nitric oxide synthase (eNOS) activity were determined after endothel...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2071-4
更新日期：2013-01-01 00:00:00
abstract：:Hsa-mir-143 and hsa-let-7c have been reported to be deregulated in multiple neoplasms. The main purpose of this study was to investigate the expression of these miRNAs in bladder cancer (BCa) and to analyze the association between their expression profiles and clinical and epidemiological parameters. Ninety BCa specim...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05228-1
更新日期：2020-02-01 00:00:00
abstract：:Histone H1 is know to play a role in the formation and maintenance of higher-order chromatin structure. It has been recently suggested that the linker histone might be also involved in the regulation of the activity of individual genes. If H1 is a regulatory factor in eukaryotic gene transcription, it should possess s...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/BF00369901
更新日期：1991-02-01 00:00:00
abstract：:Adequate metabolic adaptation of key tissues playing an essential role for bioenergetic homeostasis and lactogenesis is critical in cows to adapt to changes in energy requirements and physiological processes during the lactation period. Mitochondria are recognized as central to meet energy needs and maintaining of met...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4273-x
更新日期：2018-10-01 00:00:00
abstract：:Dipeptidyl peptidase III (DPP III) is an emerging biomarker of human cancers. Expression, specificity, and function of human DPP III (hDPP III) mRNA variant I (V-I), II (V-II), and III (V-III) are poorly understood. Here, we investigated expression of these variants in multiple human tumor derived cell lines. DNA sequ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-016-3996-9
更新日期：2016-06-01 00:00:00
abstract：:The cbb (3)-type oxidases are members of the heme-copper oxidase superfamily, distant by sequence comparisons, but sharing common functional characteristics. The cbb (3) oxidases are missing an active-site tyrosine residue that is absolutely conserved in all A and B-type heme-copper oxidases. This tyrosine is known to...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0437-z
更新日期：2011-06-01 00:00:00
abstract：:The degeneration of retinal pigment epithelium (RPE) cells in the sub retinal pigment epithelial space and choroid is an initial pathological characteristic for the age-related macular degeneration which is the leading cause of severe vision loss in old people. Moreover, oxidative stress is implicated as a major induc...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-016-4041-8
更新日期：2016-10-01 00:00:00
abstract：:Ebselen, an organoselenium compound, mimics glutathione peroxidase activity. It is a multifunctional compound, which catalyzes several essential reactions for the protection of cellular components from oxidative and free radical damage. Based on a number of in vitro and in vivo studies, various mechanisms are proposed...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-014-3417-x
更新日期：2014-08-01 00:00:00
abstract：:Acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) are common syndromes characterized by diffuse, acute injury to the alveolar epithelium and pulmonary vascular endothelial cells, with high mortality rate for there are no effective pharmacological therapies. Peroxisome proliferators-activated recept...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0647-4
更新日期：2011-11-01 00:00:00
abstract：:Ribonuclease P (RNase P) is a key enzyme involved in tRNA biosynthesis. It catalyses the endonucleolytic cleavage of nearly all tRNA precursors to produce 5'-end matured tRNA. RNase P activity has been found in all organisms examined, from bacteria to mammals. Eubacterial RNase RNA is the only known RNA enzyme which f...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/BF00988718
更新日期：1995-01-01 00:00:00
abstract：:Growing data supported that epigenetic modifications, including altered DNA methylation have a potential role in the pathogenesis of autoimmune thyroid diseases (AITD). In the present study we aimed to investigate the methylation status of ICAM-1 gene promoter in patients with AITD. Forty patients with Graves' disease...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04990-6
更新日期：2019-10-01 00:00:00
abstract：:Nonspecific lipid transfer protein (nsLTP) is usually basic and secreted low-molecular-mass protein in plants. The 3-D structure of nsLTP1 resembles that of elicitin produced by the plant pathogen Phytophthora cryptogea, which can bind to the plant plasma membrane putative receptor and activate the downstream response...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-008-9238-z
更新日期：2009-04-01 00:00:00
abstract：:Glutamate represents the main excitatory neurotransmitter in the mammalian brain; however, its excessive elevation in the extracellular space is cytotoxic and can result in neuronal death. The ischemia initiated brain damage reflects changes in glutamate concentration in peripheral blood. This paper investigated the r...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05626-w
更新日期：2020-07-01 00:00:00
abstract：:Rat liver chromatin activated by partial hepatectomy is more susceptible to the action of DNAse I than control chromatin isolated from intact liver. The study on the transfer of chromatin material to the acid-soluble fraction reveals a higher rate of activated chromatin degradation. Activated chromatin shows also an i...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00782891
更新日期：1979-12-31 00:00:00
abstract：:Sheep are valuable resources for the wool industry. Wool growth of Aohan fine wool sheep has cycled during different seasons in 1 year. Therefore, identifying genes that control wool growth cycling might lead to ways for improving the quality and yield of fine wool. In this study, we employed Agilent sheep gene expres...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3405-1
更新日期：2014-08-01 00:00:00
abstract：:Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats. We have mo...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-006-9010-1
更新日期：2007-03-01 00:00:00
abstract：:Experimental evidence highlights the importance of dietetic factors on breast cancer. In this work we aimed to analyze the effects two oils, corn oil (rich in n-6 polyunsaturated fatty acids -PUFA-) and extra virgin olive oil (EVOO), on oxidative stress in an animal model of breast carcinogenesis. Female rats were fed...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05492-6
更新日期：2020-07-01 00:00:00
abstract：:Recently, circulating miRNAs have been reported as promising biomarkers for various pathologic conditions including cancer. Certain microRNAs (miRNAs) have been shown early diagnostic potential for many types of cancer. The objective of this study was to investigate the potential of certain serum/plasma miRNAs as nove...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3322-3
更新日期：2014-07-01 00:00:00
abstract：:The complete sequence of mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis) is determined by using PCR amplification, clone and primer-walking sequencing methods. The genome is 16836 bp in size, containing 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mtDNA genome of Siamensis Crocodile...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-007-9062-x
更新日期：2008-06-01 00:00:00
