Molecular cloning and functional expression analysis of a new gene encoding geranylgeranyl diphosphate synthase from hazel (Corylus avellana L. Gasaway).

abstract：:3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors has been shown to reduce the progression of renal disease independent of cholesterol-lowering effect, but the mechanism of potential protective effect remains unclear. Here, we investigate the effect of fluvastatin on activation of nuclear factor-κB ...

journal_title：Molecular biology reports

authors： Gao P,Wu X,Shui H,Jia R

更新日期：2012-04-01 00:00:00

abstract：:Endometriosis is a polygenic and multifactorial gynecology situation which might be associated with angiogenesis. In the current study we assess the role of vascular endothelial growth factor (VEGF) - 2578 A/C, and + 936 C/T polymorphisms in susceptibility to endometriosis and checking the expression of VEGF mRNA in e...

journal_title：Molecular biology reports

authors： Rashidi BH,Sarhangi N,Aminimoghaddam S,Haghollahi F,Naji T,Amoli MM,Shahrabi-Farahani M

更新日期：2019-06-01 00:00:00

abstract：:Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys ...

journal_title：Molecular biology reports

authors： Liu H,Guo Z,Zheng H,Wang S,Wang Y,Liu W,Zhang G

更新日期：2014-11-01 00:00:00

abstract：:Three members of Brassica napus TRANSPARENT TESTA 2 (BnTT2) gene family encoding potential R2R3-MYB regulatory proteins of proanthocyanidin biosynthesis were isolated. BnTT2-1, BnTT2-2, and BnTT2-3 are 1102 bp with two introns, and have a 938-bp full-length cDNA with a 260 amino acid open reading frame. They share 98....

journal_title：Molecular biology reports

authors： Wei YL,Li JN,Lu J,Tang ZL,Pu DC,Chai YR

更新日期：2007-06-01 00:00:00

abstract：:Curcumin is a polyphenolic bioactive compound found in the spice turmeric endowed with diverse pharmacological and biological activities. In this study, fluorescence spectroscopy in combination with UV-Vis absorbance spectroscopy was employed to investigate the high affinity binding of curcumin to herring sperm DNA (h...

journal_title：Molecular biology reports

authors： Li XL,Hu YJ,Mi R,Li XY,Li PQ,Ouyang Y

更新日期：2013-07-01 00:00:00

abstract：:In this work, we identified a gene from Theobroma cacao L. genome and cDNA libraries, named TcGlu2, that encodes a β-1,3-1,4-glucanase. The TcGlu2 ORF was 720 bp in length and encoded a polypeptide of 239 amino acids with a molecular mass of 25.58 kDa. TcGlu2 contains a conserved domain characteristic of β-1,3-1,4-glu...

journal_title：Molecular biology reports

authors： Britto DS,Pirovani CP,Andrade BS,Dos Santos TP,Pungartnik C,Cascardo JC,Micheli F,Gesteira AS

更新日期：2013-09-01 00:00:00

abstract：:PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322, SV40 and bacteriophage lambda have one, two and six PaeI recognitio...

journal_title：Molecular biology reports

authors： Sokolov NN,Fitsner AB,Anikeitcheva NV,Choroshoutina YuB,Samko OT,Kolosha VO,Fodor I,Votrin II

更新日期：1985-04-01 00:00:00

abstract：:The Rv3203 (LipV) of Mycobacterium tuberculosis (Mtb) H37Rv, is annotated as a member of Lip family based on the presence of characteristic consensus esterase motif ‘GXSXG’. In vitro culture studies of Mtb H37Ra indicated that expression of Rv3203 gene was up-regulated during acidic stress as compared to normal wherea...

journal_title：Molecular biology reports

authors： Singh G,Arya S,Narang D,Jadeja D,Singh G,Gupta UD,Singh K,Kaur J

更新日期：2014-01-01 00:00:00

abstract：:Chloroplast microsatellites for two Korean endemic species, Eranthis byunsanensis and E. pungdoensis (Ranunculaceae), were isolated to address the questions of their distributional patterns and evolutionary relationships, using next-generation sequencing. Twenty-four polymorphic chloroplast microsatellite markers for ...

journal_title：Molecular biology reports

authors： Oh A,Oh BU

更新日期：2017-08-01 00:00:00

abstract：:Common bean is gaining acceptance as one of the most valuable major food consumed worldwide owing to innumerable nutritional and therapeutic benefits. Comparatively less productivity in underdeveloped countries encouraged us to proceed for QTL mining of yield traits in common bean. Heretofore, multiple yield associate...

journal_title：Molecular biology reports

authors： Gupta N,Zargar SM,Singh R,Nazir M,Mahajan R,Salgotra RK

更新日期：2020-09-01 00:00:00

abstract：UNLABELLED:Hepatocellular carcinoma (HCC) is a worldwide neoplasm for which early diagnosis is difficult and the prognosis is usually poor. Overexpression of cyclooxygenase 2 (COX-2) has been suggested to be associated with hepatocarcinogenesis. Although several COX-2 inhibitors have been used in hepatoma therapy, the ...

journal_title：Molecular biology reports

authors： Mohamed FZ,Hussein YM,El-Deen IM,Sabea MS

更新日期：2014-03-01 00:00:00

abstract：:Staudtia kamerunensis (Myristicaceae) or 'Niové' is an evergreen tree widespread in Central African moist forests. The bark and seeds are used in traditional medicine, yet the tree is mainly harvested for its high quality, multi-purpose timber. To facilitate sustainable harvesting and conservation of the species, we a...

journal_title：Molecular biology reports

authors： Vanden Abeele S,Hardy OJ,Janssens SB

更新日期：2018-10-01 00:00:00

abstract：:The recombinant catalytic subunit of human protein kinase CK2 bas been mutagenised at the C-terminal region in an attempt to induce this tail to fold. We suppose in fact that this unstructured C-terminus just might be responsible for the high degradability of the human enzyme. On the basis of theoretical calculations ...

journal_title：Molecular biology reports

authors： Grasselli E,Noviello G,Rando C,Nicolini C,Vergani L

更新日期：2003-06-01 00:00:00

abstract：:An improvement in the detection by nucleic acid hybridization of size-fractionated RNA immobilized to nylon-based membranes is described. Electrophoretic transfer of RNA to nylon membranes permits a quantitative determination of different RNA transcripts on the same membrane after sequential hybridization using differ...

journal_title：Molecular biology reports

authors： Khandjian EW

更新日期：1986-01-01 00:00:00

abstract：:Repeated implantation failure (RIF) is one the most common causes which showed during IVF (In vitro fertilization) procedure. We aim to evaluate the possibility role of nucleotide changes in rs1042522 (R72P; G/C) and rs17878362 (Ins16bp; N/D) of P53 gene in patients with RIF. In a case-control survey, we have consider...

journal_title：Molecular biology reports

authors： Mohammadzadeh M,Ghorbian S,Nouri M

更新日期：2019-06-01 00:00:00

abstract：:Diabetic retinopathy (DR) is a multifactorial disease with complex pathophysiology. It is the main cause of blindness among the people in productive age. The purpose of this literature review is to highlight recent achievements in the genetics of diabetic retinopathy with particular focus on candidate gene studies. We...

journal_title：Molecular biology reports

authors： Priščáková P,Minárik G,Repiská V

更新日期：2016-12-01 00:00:00

abstract：:CyclinD1 over-expression is the key pathogenetic event underlying airway smooth muscle (ASM) proliferation. Human antigen R (HuR) is a ubiquitously expressed RNA-binding protein, and is known to regulate the expression of multiple cell cycle regulators. The aim of the study is to investigate whether HuR might also be ...

journal_title：Molecular biology reports

authors： Zhang P,Cao M,Liu Y,Lv Z,Yang Q,Lin X,Li H,Wan Q

更新日期：2012-05-01 00:00:00

abstract：:Recently, circulating miRNAs have been reported as promising biomarkers for various pathologic conditions including cancer. Certain microRNAs (miRNAs) have been shown early diagnostic potential for many types of cancer. The objective of this study was to investigate the potential of certain serum/plasma miRNAs as nove...

journal_title：Molecular biology reports

authors： Giray BG,Emekdas G,Tezcan S,Ulger M,Serin MS,Sezgin O,Altintas E,Tiftik EN

更新日期：2014-07-01 00:00:00

abstract：:In the present study, root cell suspension cultures of W. somnifera were elicited with mycelial extract (1% w/v) and culture filtrate (5% v/v) of their native endophytic fungus Aspergillus terreus 2aWF in shake flask. Culture filtrate of A. terreus 2aWF significantly elicits withanolide A at 6H (12.20 ± 0.52 µg/g FCB)...

journal_title：Molecular biology reports

authors： Kushwaha RK,Singh S,Pandey SS,Kalra A,Vivek Babu CS

更新日期：2019-04-01 00:00:00

abstract：:Groups of CBA mice were administered [35S] methionine (1 mCi/mouse). Non-histone proteins, H1 and H1(0) histones and nucleosomal core histones were isolated from different issues by selective extractions. The measurements of radioactivity of individual bands and autoradiography of dry gels were used to identify methio...

journal_title：Molecular biology reports

authors： Medvedev ZA,Medvedeva MN

更新日期：1988-01-01 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy is a useful biophysical technique to study the ligand-protein interaction. In this report, we have used bacterially produced ERβ and its domains for studying the functional analysis of ligand-protein interaction. Briefly, ERβ and its transactivation domain (TAD) and ligand...

journal_title：Molecular biology reports

authors： Paramanik V,Thakur MK

更新日期：2011-10-01 00:00:00

abstract：:Cartilage has a limited self-repair capability and the repair of large cartilage defects remains a challenge in clinic. This study aimed to investigate the effect of mesenchymal stem cells (MSCs) loaded three-dimensional bilayer collagen scaffold for cartilage repair. Cross-linked three-dimensional bilayer collagen sc...

journal_title：Molecular biology reports

authors： Qi Y,Zhao T,Xu K,Dai T,Yan W

更新日期：2012-02-01 00:00:00

abstract：:The investigation was carried out to analyse the genetic polymorphism and gene expression of ATP1A1 gene in four different Indian goat breeds by using high resolution melting (HRM) and real time-PCR. ATPase is electro-genic ion pump which is maintains the balance of sodium and potassium ions in animal cells. The trans...

journal_title：Molecular biology reports

authors： Kaushik R,Goel A,Rout PK

更新日期：2019-10-01 00:00:00

abstract：:Proteasomes (prosomes) are large multiprotein complexes. They are involved in protein degradation of ubiquitin-conjugated proteins and in the generation of MHC class I peptides. We gave further evidence that they interfere with in vitro protein synthesis. Proteasomes inhibit the translation of Tobacco mosaic virus RNA...

journal_title：Molecular biology reports

authors： Homma S,Horsch A,Pouch MN,Petit F,Briand Y,Schmid HP

更新日期：1994-01-01 00:00:00

abstract：:Interferon gamma (IFN-γ) is an important immunoregulatory cytokine that has a central role against viral and bacterial infections. In this study, the cDNA encoding 141 amino acids of mature IFN-γ from mice splenocytes was cloned in a prokaryotic expression vector pQE 30. Optimization of expression conditions resulted ...

journal_title：Molecular biology reports

authors： Kumar M,Singh M,Singh SB

更新日期：2014-10-01 00:00:00

abstract：:Radiotherapy is an effective treatment for some esophageal cancers, but the molecular mechanisms of radiosensitivity remain unknown. Ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is a novel nuclear protein which is overexpressed in various cancers but not yet examined in esophageal squamous cell carcinoma ...

journal_title：Molecular biology reports

authors： Yang C,Wang Y,Zhang F,Sun G,Li C,Jing S,Liu Q,Cheng Y

更新日期：2013-09-01 00:00:00

abstract：:The transforming growth factor-β (TGF-β) signaling pathway is conserved across animals, and knowledge of its roles during the molt cycle in crustaceans is presently very limited. This study investigates the roles of the TGF-β receptor in molting-related muscle growth in Eriocheir sinensis. Using the RT-PCR and RACE te...

journal_title：Molecular biology reports

authors： Tian Z,Peng H,Deng W,Jiao C

更新日期：2020-01-01 00:00:00

abstract：:Trichoderma asperellum, a filamentous soil fungus, is an effective biocontrol agent against many fungal plant pathogenic species. In the present study, we investigated the biological control properties of the strain T. asperellum T4. T. asperellum fermentation products significantly decreased the ability of Rhizoctoni...

journal_title：Molecular biology reports

authors： Liu Z,Yang X,Sun D,Song J,Chen G,Juba O,Yang Q

更新日期：2010-12-01 00:00:00

abstract：:Vitiligo is an acquired pigmentary disorder characterized by loss of epidermal melanocytes. A strong association at a single nucleotide polymorphism (SNP) rs11966200 within MHC region had been identified in a recent genome-wide association study of generalized vitiligo in Chinese Han population. This study aims to inv...

journal_title：Molecular biology reports

authors： Tang J,Liu JL,Zhang C,Hu DY,He SM,Zuo XB,Wang PG,Sun LD,Zhang XJ,Yang S

更新日期：2013-06-01 00:00:00

abstract：:Proto-oncogen Ahi-1 is closely related to a lot of human and mouse diseases. Ahi-1 mutation will lead to leukemia in mice and Joubert syndrome in human beings. We have cloned the full cDNA sequence of Ahi-1 homologous in zebrafish, and RT-PCR results of ZAhi-1 in different tissues reveal that ZAhi-1 expressed highest ...

journal_title：Molecular biology reports

authors： Zhou W,Song P

更新日期：2006-06-01 00:00:00