Abstract:
:PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322, SV40 and bacteriophage lambda have one, two and six PaeI recognition sites, respectively. Seventy-two strains of Pseudomonas, Clostridium, Escherichia coli, Shigella, Proteus and Saccharomyces were screened for the presence of site-specific endonucleases. Here we describe the PaeI restriction enzyme found in Pseudomonas aeruginosa; other data will be published elsewhere. Earlier Hinkle and Miller isolated from P. aeruginosa a PaeR7 restriction endonuclease recognizing and cleaving a sequence 5'-C reduced TCGAG-3' (1). Sequence analysis of DNAs cleaved by PaeI shows that the enzyme is the isoschizomer of SphI (2).
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Sokolov NN,Fitsner AB,Anikeitcheva NV,Choroshoutina YuB,Samko OT,Kolosha VO,Fodor I,Votrin IIdoi
10.1007/BF00778522subject
Has Abstractpub_date
1985-04-01 00:00:00pages
159-61issue
3eissn
0301-4851issn
1573-4978journal_volume
10pub_type
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