Cloning and characterization of Na+/H+ antiporter (LfNHX1) gene from a halophyte grass Leptochloa fusca for drought and salt tolerance.

abstract：:The complement factor H (CFH) Y402H (T1277C) gene polymorphism has been reported to be associated with coronary heart disease (CHD), but results were conflicting. To evaluate the role of the variant in CHD, we performed meta-analyses of all available data. Both electronic and manual searches were performed, all releva...

journal_title：Molecular biology reports

authors： Zhang HF,Wang JF,Wang Y,Zhu LG,Lei L

更新日期：2011-06-01 00:00:00

abstract：:Spata34 is a testis-specific-expressed gene which exerts diverse functions in testis development. This study intends to examine the expression profiles of Spata34 in postnatal rat testis, and explore its potential roles in cell proliferation in vitro. We found that the mRNA and protein expression levels of Spata34 wer...

journal_title：Molecular biology reports

authors： Nie D,Li H,Liu Y,Liao Z

更新日期：2018-12-01 00:00:00

abstract：:The purpose was to analyze the growth hormone GH1/GH2-N and GH2-Z gene copies and to assess their possible association with milk traits in Sarda sheep. Two hundred multiparous lactating ewes were monitored. The two gene copies were amplified separately and each was used as template for a nested PCR, to investigate sin...

journal_title：Molecular biology reports

authors： Vacca GM,Dettori ML,Balia F,Luridiana S,Mura MC,Carcangiu V,Pazzola M

更新日期：2013-09-01 00:00:00

abstract：:Osteoporosis is a degenerative disease of the skeletal system, and its major complication is fracture that severely influences the living quality of the middle-aged and the aged. The purpose of this study was to investigate the significance of sex hormones and some biochemical indicators related to bone metabolism in ...

journal_title：Molecular biology reports

authors： Liu SZ,Tian LF,Xu P,Zhuang GH,Zheng F,Tian J,Ning QL,Zhu BF,Lu SM,Yan H

更新日期：2011-02-01 00:00:00

abstract：:The novel dipstick DNA extraction method was tested for its reliability and usability for biological barcoding in comparison to a commercial kit and to a simplified isopropanol precipitation method using crayfish gill tissue. Following DNA extraction, the mitochondrial COI-gene was amplified in a PCR-reaction using a ...

journal_title：Molecular biology reports

authors： Martello A,Lambert B,Johnston C,Cutler J,Stumpf CF

更新日期：2019-12-01 00:00:00

abstract：:Tumor angiogenesis, a major requirement for tumor growth and metastasis, is regulated by pro- and anti-angiogenic factors. The aim of this study was to quantify the expression of angiogenic (VEGF, HIF-1α, Angiopiotein-2) and anti-angiogenic (endostatin, angiostatin and Thrombospondin-1) factors and to discern their cl...

journal_title：Molecular biology reports

authors： Sharma BK,Srinivasan R,Kapil S,Singla B,Chawla YK,Chakraborti A,Saini N,Duseja A,Das A,Kalra N,Dhiman RK

更新日期：2013-10-01 00:00:00

abstract：:Growth-regulating factor (GRF) is an important protein in GA-mediated response, with key roles in plant growth and development. However, it is not known whether or how the GRF proteins in citrus to regulate organ size. In this study, nine citrus GRF genes (CsGRF1-9) were validated from the 'Anliu' sweet orange (AL, Ci...

journal_title：Molecular biology reports

authors： Liu X,Guo LX,Jin LF,Liu YZ,Liu T,Fan YH,Peng SA

更新日期：2016-10-01 00:00:00

abstract：:The cell divisions cycle 42 (Cdc42) gene has been characterized in the fungi, such as Candida albicans, Penicillium marneffei, and Wangiella (Exophiala) dermatitidis, which plays important roles during growth and development. The partial cDNA sequence of Cdc42 of Fonsecaea monophora (F. monophora), designated FmCdc42,...

journal_title：Molecular biology reports

authors： Xie Z,Feng P,Zhang J,Li X,Sun J,Lu C,Huang H,Xi L

更新日期：2012-02-01 00:00:00

abstract：:The kelch-repeat protein family is a recently found new kind of actin-binding protein. It is characterized by tandemly arranged motifs of about 50 amino acids. Previous study showed that most members of the kelch-repeat family were cytoskeletal proteins implicated in various cellular processes, such as actin cytoskele...

journal_title：Molecular biology reports

authors： Xu J,Gu S,Wang S,Dai J,Ji C,Jin Y,Qian J,Wang L,Ye X,Xie Y,Mao Y

更新日期：2003-12-01 00:00:00

abstract：:Cell free protein synthesis has become a powerful method for the high-throughput production of proteins that are difficult to express in living cells. The protein SAP2 of Fasciola hepatica (FhSAP2), which has demonstrated to be both, an excellent vaccine candidate against experimental fascioliasis and a good antigen f...

journal_title：Molecular biology reports

authors： Ramos-Benítez MJ,Lopez-Cruz LM,Aguayo V,Ruiz-Jimenez C,Espino AM

更新日期：2018-10-01 00:00:00

abstract：:Several studies have examined the effects of the tumor necrosis factor-α (TNF-α) -308 A/G and lymphotoxin-α (LT-α) +252 A/G polymorphisms on susceptibility to sarcoidosis, showing mixed results. The purpose of this study was to examine whether the TNF-α -308 A/G and LT-α +252 A/G polymorphisms confer susceptibility to...

journal_title：Molecular biology reports

authors： Song GG,Kim JH,Lee YH

更新日期：2014-01-01 00:00:00

abstract：:Methylenetetrahydrofolate reductase (MTHFR) polymorphism may play an important role in the pathophysiology of obesity and diabetes accompanied by obesity due to its influence on plasma homocysteine levels. There are significant and sometimes very strong relationship between levels of homocysteine and several multi-sys...

journal_title：Molecular biology reports

authors： Tavakkoly Bazzaz J,Shojapoor M,Nazem H,Amiri P,Fakhrzadeh H,Heshmat R,Parvizi M,Hasani Ranjbar S,Amoli MM

更新日期：2010-01-01 00:00:00

abstract：:Beta(2)-microglobulin (beta(2)m), a protein necessary for proper folding, peptide binding, and surface display of class I antigens plays an important role in immune response. The full-length cDNA containing beta(2)m was cloned from the spleen cDNA library of large yellow croaker Pseudosciaena crocea (Pscr-beta ( 2 ) m...

journal_title：Molecular biology reports

authors： Yu S,Chen X,Ao J

更新日期：2009-09-01 00:00:00

abstract：:The product of the OTUB1 gene is a member of the OTU superfamily of predicted cysteine proteases and inhibits cytokine gene transcription via its interaction with a ubiquitin protease and E3 ubiquitin ligase. To further understand the functions of the porcine OTUB1 gene, the subcellular localization of porcine OTUB1 p...

journal_title：Molecular biology reports

authors： Shan TL,Tang ZL,Guo DZ,Yang SL,Mu YL,Ma YH,Guan WJ,Li K

更新日期：2009-07-01 00:00:00

abstract：:Patients with β-thalassemia suffer from a lack or absence of the beta-globin chain of normal hemoglobin (Hb). Therefore, an increase in fetal Hb (HbF) levels could improve the clinical status of these patients. Downregulation of BCL11A, a key regulatory transcription factor, could ameliorate the clinical status of tha...

journal_title：Molecular biology reports

authors： Gholampour MA,Asadi M,Naderi M,Azarkeivan A,Soleimani M,Atashi A

更新日期：2020-05-01 00:00:00

abstract：:Cardiomyocytes possess the ability to respond to mechanical stimuli by reprogramming their gene expression. This study investigated the effects of different loading protocols on signaling and expression responses of myogenic, anabolic, inflammatory, atrophy and pro-apoptotic genes in cardiomyocyte-like H9C2 cells. Dif...

journal_title：Molecular biology reports

authors： Zevolis E,Philippou A,Moustogiannis A,Chatzigeorgiou A,Koutsilieris M

更新日期：2021-01-04 00:00:00

abstract：:The glutathione S-transferase (GST) family of enzymes is known to play a pivotal role in phase II of biotransformation of xenobiotics, environmental carcinogens and pharmacological drugs. The objective of the present study was to investigate the role of GSTM1 and GSTT1 null genotypes as risk factors for chronic obstru...

journal_title：Molecular biology reports

authors： Thakur H,Gupta L,Sobti RC,Janmeja AK,Seth A,Singh SK

更新日期：2011-03-01 00:00:00

abstract：:Interleukin-13 (IL-13) is a TH2 cytokine with direct and indirect immunoregulatory functions on cancer cells. The cytokine has been reported to have some polymorphic variations at the gene level associated with some immune related diseases including asthma and allergy. In the present study, association of three IL13 g...

journal_title：Molecular biology reports

authors： Faghih Z,Erfani N,Razmkhah M,Sameni S,Talei A,Ghaderi A

更新日期：2009-09-01 00:00:00

abstract：:Anti-EGFR monoclonal antibodies (anti-EGFR MoAbs) in metastatic colorectal cancer (mCRC) treatment are still not effective in all patients. This study aimed to evaluate the relationship between BRAF V600E mutation and the tumor response of anti-EGFR MoAbs for first-line treatment in mCRC patients. We searched the MEDL...

journal_title：Molecular biology reports

authors： Cui D,Cao D,Yang Y,Qiu M,Huang Y,Yi C

更新日期：2014-03-01 00:00:00

abstract：:The interaction between ovalbumin (OVA) and three purine alkaloids (caffeine, theophylline and diprophylline) was investigated by the aid of intrinsic and synchronous fluorescence, ultraviolet-vis absorbance, resonance light-scattering spectra and three-dimensional fluorescence spectra techniques. Results showed that ...

journal_title：Molecular biology reports

authors： Wang RQ,Yin YJ,Li H,Wang Y,Pu JJ,Wang R,Dou HJ,Song CJ,Wang RY

更新日期：2013-04-01 00:00:00

abstract：:Histone H1 is know to play a role in the formation and maintenance of higher-order chromatin structure. It has been recently suggested that the linker histone might be also involved in the regulation of the activity of individual genes. If H1 is a regulatory factor in eukaryotic gene transcription, it should possess s...

journal_title：Molecular biology reports

authors： Zlatanova J,Yaneva J

更新日期：1991-02-01 00:00:00

abstract：:Metallothioneins are rather ubiquitous metal-binding proteins induced by stressing or physiological stimuli. Two major metallothionein isoforms have been identified in mussel: MT10 and MT20. Nevertheless the high sequence homology, the two isoforms exhibit different expression and inducibility in vivo. We cloned and p...

journal_title：Molecular biology reports

authors： Grattarola M,Carloni M,Dondero F,Viarengo A,Vergani L

更新日期：2006-12-01 00:00:00

abstract：:The complete coding sequences of three porcine genes-SDHB, SNRPA and CRYBB1 were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the mouse or other mammals and highly homologous pig ESTs. These three genes were then deposited into GenBa...

journal_title：Molecular biology reports

authors： An QC,Liu GY

更新日期：2009-04-01 00:00:00

abstract：:Intrinsic bent DNA sites were identified in the 4289 bp segment encompassing the replication zone which directs DNA amplification and transcription of the C3-22 gene of Rhynchosciara americana. Restriction fragments showed reduced electrophoretic mobility in polyacrylamide gels. The 2D modeling of the 3D DNA path and ...

journal_title：Molecular biology reports

authors： Fiorini A,de Gouveia FS,de Soares MA,Stocker AJ,Ciferri RR,Fernandez MA

更新日期：2006-03-01 00:00:00

abstract：:Microsatellites, or simple sequence repeats (SSRs), are very useful molecular markers for a number of plant species. We used a new publicly available module (TROLL) to extract microsatellites from the public database of soybean expressed sequence tag (EST) sequences. A total of 12,833 sequences containing di- to penta...

journal_title：Molecular biology reports

authors： Xin D,Sun J,Wang J,Jiang H,Hu G,Liu C,Chen Q

更新日期：2012-09-01 00:00:00

abstract：:In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis-inducing protein, as a GST-fusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death. The HAP protein inhibited the bacterial growth within 30 min after inducting HAP expression. The transmission e...

journal_title：Molecular biology reports

authors： Gan M,Qi Y,Wan Q,Kuang E,Liu Q,Liu X

更新日期：2004-09-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) has been investigated extensively at the molecular level. Nevertheless, genome wide study on the temporal and spatial dynamics of the virus is non-existed. To explore this topic, we determined complete coding genome sequences for four PRRSV isolates and analy...

journal_title：Molecular biology reports

authors： Yoon SH,Kim H,Kim J,Lee HK,Park B,Kim H

更新日期：2013-10-12 00:00:00

abstract：:Safe harbor loci allow predicable integration of a transgene into the genome without perturbing endogenous gene activity and for decades have been exploited in the mouse to investigate gene function, generate humanised models and create tissue specific reporter and Cre recombinase expressing lines. Herein, we show tha...

journal_title：Molecular biology reports

authors： Browning J,Rooney M,Hams E,Takahashi S,Mizuno S,Sugiyama F,Fallon PG,Kelly VP

更新日期：2020-02-01 00:00:00

abstract：:The full-length MECPS cDNA sequence (designated as Chmecps, GenBank Accession No.: DQ415658) was isolated by rapid amplification of cDNA ends (RACE) for the first time from Cephalotaxus harringtonia. The full-length cDNA of Chmecps was 1,146 bp containing a 753 bp open reading frame (ORF) encoding a polypeptide of 250...

journal_title：Molecular biology reports

authors： Chen J,Xiao Y,Di P,Yu X,Chen W,Zhang L

更新日期：2009-09-01 00:00:00

abstract：:The mRNA differential display technique was performed to investigate the differences of gene expression in the longissimus muscle tissues from Meishan and Large White pigs. One novel mRNA that was differentially expressed was identified through semi-quantitative RT-PCR and the cDNA complete sequence was then obtained ...

journal_title：Molecular biology reports

authors： Liu GY,Xiong YZ

更新日期：2009-07-01 00:00:00