Abstract:
:Cardiomyocytes possess the ability to respond to mechanical stimuli by reprogramming their gene expression. This study investigated the effects of different loading protocols on signaling and expression responses of myogenic, anabolic, inflammatory, atrophy and pro-apoptotic genes in cardiomyocyte-like H9C2 cells. Differentiated H9C2 cells underwent various stretching protocols by altering their elongation, frequency and duration, utilizing an in vitro cell tension system. The loading-induced expression changes of MyoD, Myogenin, MRF4, IGF-1 isoforms, Atrogin-1, Foxo1, Fuca and IL-6 were measured by Real Time-PCR. The stretching-induced activation of Akt and Erk 1/2 was also evaluated by Western blot analysis. Low strain (2.7% elongation), low frequency (0.25 Hz) and intermediate duration (12 h) stretching protocol was overall the most effective in inducing beneficial responses, i.e., protein synthesis along with the suppression of apoptosis, inflammation and atrophy, in the differentiated cardiomyocytes. These findings demonstrated that varying the characteristics of mechanical loading applied on H9C2 cells in vitro can regulate their anabolic/survival program.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Zevolis E,Philippou A,Moustogiannis A,Chatzigeorgiou A,Koutsilieris Mdoi
10.1007/s11033-020-06112-zsubject
Has Abstractpub_date
2021-01-04 00:00:00eissn
0301-4851issn
1573-4978pii
10.1007/s11033-020-06112-zpub_type
杂志文章abstract::The lily-of-the-valley Convallaria (Asparagaceae) consists of three herbaceous perennial species. The plants are commonly found in northern hemisphere, and are best-known for their ornamental and pharmaceutical value. In order to assess the genetic structure, diversity and demographic history of Convallaria species, 1...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05376-9
更新日期:2020-04-01 00:00:00
abstract::Common bean is gaining acceptance as one of the most valuable major food consumed worldwide owing to innumerable nutritional and therapeutic benefits. Comparatively less productivity in underdeveloped countries encouraged us to proceed for QTL mining of yield traits in common bean. Heretofore, multiple yield associate...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05735-6
更新日期:2020-09-01 00:00:00
abstract::Immense body of evidence indicates that dysfunction of immune system is implicated in the etiology of schizophrenia. The immune theory of schizophrenia is supported by alterations in cytokine profile in the brain and peripheral blood. Given the strong genetic background of schizophrenia, it might be assumed that aberr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2662-8
更新日期:2013-10-01 00:00:00
abstract::Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0736-z
更新日期:2012-01-01 00:00:00
abstract::One mutant transgenic line displaying homeotic conversion of sepals to petals with other phenotypic aberrations was selected and characterized at molecular level. The increased transcript level of gene encoding anthocyanidin synthase and petal specific class B genes, GLOBOSA and DEFECIENS in sepals of mutant line may ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2909-4
更新日期:2014-02-01 00:00:00
abstract::Master differentiation transcription factors (MDFs) play decisive roles in cell lineage commitment and cellular functions. Forkhead box P3 (Foxp3) is the MDF essential for the lineage commitment and the suppression function of regulatory T cells (Tregs), which play critical roles in suppression of autoimmunity. Here, ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1825-3
更新日期:2012-10-01 00:00:00
abstract::Recently genetics and epigenetics alterations have been found to be characteristic of malignancy and hence can be used as targets for detection of neoplasia. RAS association domain family protein 1A (RASSF1A) gene hypermethylation has been a subject of interest in recent researches on cancer breast patients. The aim o...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2837-3
更新日期:2014-01-01 00:00:00
abstract::The cytotoxic T lymphocyte-associated antigen 4 gene (CTLA4) has a crucial role in regulation of T cell proliferation and mediates T cell apoptosis by encoding the T cell receptor. Schizophrenia (SCZ) patients often have abnormalities in terms of the function and development of the immune system. The aim of the presen...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2614-3
更新日期:2013-08-01 00:00:00
abstract::Nonspecific lipid transfer protein (nsLTP) is usually basic and secreted low-molecular-mass protein in plants. The 3-D structure of nsLTP1 resembles that of elicitin produced by the plant pathogen Phytophthora cryptogea, which can bind to the plant plasma membrane putative receptor and activate the downstream response...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9238-z
更新日期:2009-04-01 00:00:00
abstract::DNA polymerase alpha from germinated wheat embryos was purified by ammonium sulphate fractionation, chromatography on DEAE-Toyopearl, followed by phosphocellulose and heparin Sepharose columns. The specific activity of the purified enzyme was more than 60,000 units/mg. It belongs to the alpha-type according to the lar...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00788748
更新日期:1992-02-01 00:00:00
abstract::Expressed sequence tags (ESTs) provide valuable tools that can be used to predict the genes involved in primary and secondary metabolite synthesis. To the best of our knowledge, ESTs have not yet been developed for Codonopsis. lanceolata, and therefore, the EST referenced in this report is the first transcript for C. ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0464-9
更新日期:2011-06-01 00:00:00
abstract::Ran is a multifunctional small GTPase involved in important cellular activities like nucleocytoplasmic transport, mitotic spindle assembly, nuclear envelope formation, etc., but is also known to be differentially expressed in response to abiotic stress, particularly low temperature. We have over-expressed Lepidium lat...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3476-z
更新日期:2014-09-01 00:00:00
abstract::The studies conducted aimed at evaluating the genetic diversity within and between varieties of conservative flocks of geese, using the polymorphism of 14 microsatellite sequences. The experimental material included conservative flocks of geese the following indigenous breeds and varieties kept in Poland: Kielecka (Ki...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1327-8
更新日期:2012-05-01 00:00:00
abstract::The interferon-inducible human MxA protein plays an important role in innate defense against an array of viruses. One might expect allelic diversity at the MxA locus to influence the timing and magnitude of its expression or even the range of viruses whose biological cycle is inhibited by the encoded product. Here we ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1708-7
更新日期:2012-08-01 00:00:00
abstract::This experiment was conducted to explore the biological functions of myogenin (MyoG) gene. MyoG gene was cloned from genome of Hu sheep by overlap extension PCR. Then, pEGFP-C1-MyoG and pcDNA3.0-MyoG fusion expression vectors was constructed and pEGFP-C1-MyoG vector had been transfected into NIH-3T3 cells by liposomes...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2945-0
更新日期:2014-02-01 00:00:00
abstract::The round-leaf sundew (Drosera rotundifolia L.) is a carnivorous plant expressing a wide range of chitinolytic enzymes playing role in many different processes. In this study the intact plants were analyzed for the presence of chitinase transcripts and chitinolytic activities in different organs. In situ hybridization...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9254-z
更新日期:2009-05-01 00:00:00
abstract::Male Breast Cancer (MBC) is a rare and aggressive disease that is associated with genetic factors. Mutations in BRCA1 and BRCA2 account for 10% of all MBC cases suggesting that other genetic factors are involved. The aim of the present study is to screen whole BRCA1 and BRCA2 exons using the Ampliseq BRCA panel in Tun...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05703-0
更新日期:2020-09-01 00:00:00
abstract::A Cys2/His2-type zinc finger protein gene, DgZFP, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach. The DgZFP encodes a protein of 211 amino acids residues with a calculated molecular mass of 22.9 kDa and theoretical isoelectric point is 8.59. DgZFP contains two Cys2/His2-type zinc f...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9886-7
更新日期:2010-02-01 00:00:00
abstract::The study was done to identify the candidate causal single nucleotide polymorphisms (SNPs) and candidate causal mechanisms that contribute to Parkinson's disease (PD) susceptibility and to generate a SNP to ene to pathway hypothesis using an analytical pathway-based approach. We used a PD genome-wide association study...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-2346-9
更新日期:2013-03-01 00:00:00
abstract::Maize is one of the world's most important crops and a model for grass genome research. Long terminal repeat (LTR) retrotransposons comprise most of the maize genome; their ability to produce new copies makes them efficient high-throughput genetic markers. Inter-retrotransposon-amplified polymorphisms (IRAPs) were use...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05246-4
更新日期:2020-03-01 00:00:00
abstract::3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors has been shown to reduce the progression of renal disease independent of cholesterol-lowering effect, but the mechanism of potential protective effect remains unclear. Here, we investigate the effect of fluvastatin on activation of nuclear factor-κB ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1264-6
更新日期:2012-04-01 00:00:00
abstract::Increasing numbers of reports show that imprinted genes play a crucial role in fetal development, and uniparental embryos, which possess two paternally or two maternally derived pronuclei, are excellent tools for investigating the biological significance of imprinted genes. In the present study, to examine the in vitr...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3774-5
更新日期:2015-02-01 00:00:00
abstract::Hypertension is a risk factor for the cardiovascular diseases. Although, several drugs are used to treat hypertension, the success of the antihypertensive therapy is limited. Resveratrol decreases blood pressure in animal models of hypertension. This study researched the mechanisms behind the effects of resveratrol on...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3737-x
更新日期:2015-01-01 00:00:00
abstract::A secretory high-level expression cloning vector designated as pSBC-20 was constructed by inserting a DNA fragment encoding the signal peptide of ompA protein into pBV 220 vector. Any foreign DNA fragment can be inserted into the polylinker cloning sites located after the secretion signal sequence. The cloned foreign ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00986500
更新日期:1995-01-01 00:00:00
abstract::Heavy metals pollution affects the nutritive value of fish. This study examined if the inclusion of dietary hempseed (HS) and hempseed oil (HO) in the diet of the fish could revert the copper-induced toxic effects on muscle fatty acid profile of rohu (Labeo rohita) and mrigal (Cirrhinus mrigala). Fingerlings of both s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4483-2
更新日期:2019-02-01 00:00:00
abstract::The small molecules already existing on the earth can be assembled to biological macromolecules in the presence of the suitable tool. A workman must sharpen his tools if he is to do his work well. The tool must be specific, delicate and automatic. Obviously, it is enzyme. Therefore, to explore the origin of life we mu...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9189-9
更新日期:2009-02-01 00:00:00
abstract::Quinone oxidoreductases (NAD(P)H): quinone oxidoreductase 1 (NQO1) and NRH: quinone oxidoreductase 2 (NQO2) are an antioxidant enzyme, important in the detoxification of environmental carcinogens. Methylene-tetra-hydrofolate reductase (MTHFR), plays a role in folate metabolism and may have oncogenic role through disru...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2023-z
更新日期:2012-12-01 00:00:00
abstract::The tertiary structure of nucleic acids results from an equilibrium between electrostatic interactions of phosphates, stacking interactions of bases, hydrogen bonds between polar atoms and water molecules. Water interactions with ribonucleic acid play a key role in its structure formation, stabilization and dynamics. ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1452-z
更新日期:2012-05-01 00:00:00
abstract::In order to extend the knowledge of rabies pathogenesis, a two-dimensional electrophoresis/mass spectrometry based postmortem comparative proteomics analysis was carried out on human brain samples. Alteration in expression profile of several proteins was detected. Proteins related to cytoskeleton, metabolism, proteaso...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2759-0
更新日期:2013-11-01 00:00:00
abstract::The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vect...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9851-5
更新日期:2010-07-01 00:00:00