Isolation of DNA polymerase alpha from germinated wheat embryos.

Abstract:

:DNA polymerase alpha from germinated wheat embryos was purified by ammonium sulphate fractionation, chromatography on DEAE-Toyopearl, followed by phosphocellulose and heparin Sepharose columns. The specific activity of the purified enzyme was more than 60,000 units/mg. It belongs to the alpha-type according to the large molecular mass, high sensitivity to NEM, aphidicoline, 200 mM KCl, low sensitivity to ethidium bromide and the absence of inhibition by ddTTP. DNA polymerase alpha consists of four subunits as shown by SDS-PAGE and seems to be homogeneous under non-denaturing conditions.

journal_name

Mol Biol Rep

authors

Balmukhanov TS,Erekenov AM,Ajtkhozhina NA

doi

10.1007/BF00788748

keywords:

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

11-6

issue

1

eissn

0301-4851

issn

1573-4978

journal_volume

16

pub_type

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