Abstract：
:DNA polymerase alpha from germinated wheat embryos was purified by ammonium sulphate fractionation, chromatography on DEAE-Toyopearl, followed by phosphocellulose and heparin Sepharose columns. The specific activity of the purified enzyme was more than 60,000 units/mg. It belongs to the alpha-type according to the large molecular mass, high sensitivity to NEM, aphidicoline, 200 mM KCl, low sensitivity to ethidium bromide and the absence of inhibition by ddTTP. DNA polymerase alpha consists of four subunits as shown by SDS-PAGE and seems to be homogeneous under non-denaturing conditions.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Balmukhanov TS,Erekenov AM,Ajtkhozhina NAdoi
10.1007/BF00788748keywords:
subject
Has Abstractpub_date
1992-02-01 00:00:00pages
11-6issue
1eissn
0301-4851issn
1573-4978journal_volume
16pub_type
杂志文章abstract：:Se is an essential trace element and is found as a selenocysteine in the active site of Se-enzymes, such as glutathione peroxidase. tRNASec is first aminoacylated with serine by Ser RS and further is converted to selenocysteyl-tRNA by selenocysteine synthase. Mammalian selenocysteine tRNA has dual identities with Ser ...
journal_title：Molecular biology reports
pub_type： 杂志文章
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更新日期：1998-11-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04682-1
更新日期：2019-04-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11033-020-05974-7
更新日期：2020-12-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-007-9204-1
更新日期：2009-03-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4370-x
更新日期：2018-12-01 00:00:00
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更新日期：2014-05-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0080-8
更新日期：2011-01-01 00:00:00
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更新日期：2020-05-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00357405
更新日期：1973-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11033-010-0258-0
更新日期：2011-03-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1007/s11033-020-05911-8
更新日期：2020-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1776-8
更新日期：2012-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11033-020-05840-6
更新日期：2020-10-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0260-6
更新日期：2011-03-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9500-z
更新日期：2010-01-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1070-1
更新日期：2012-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11033-010-0587-z
更新日期：2011-10-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1020318705894
更新日期：2002-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11033-014-3833-y
更新日期：2015-05-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11033-012-2327-z
更新日期：2013-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-06-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4563-3
更新日期：2019-02-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05246-4
更新日期：2020-03-01 00:00:00
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journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1020317610863
更新日期：2002-01-01 00:00:00
abstract：:Spata34 is a testis-specific-expressed gene which exerts diverse functions in testis development. This study intends to examine the expression profiles of Spata34 in postnatal rat testis, and explore its potential roles in cell proliferation in vitro. We found that the mRNA and protein expression levels of Spata34 wer...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-018-4439-6
更新日期：2018-12-01 00:00:00
abstract：:The recombinant catalytic subunit of human protein kinase CK2 bas been mutagenised at the C-terminal region in an attempt to induce this tail to fold. We suppose in fact that this unstructured C-terminus just might be responsible for the high degradability of the human enzyme. On the basis of theoretical calculations ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1023934805326
更新日期：2003-06-01 00:00:00
