Abstract:
:PCR low ionic strength single-strand conformation polymorphism (PCR-LIS-SSCP) and DNA sequencing methods were used to analyze the polymorphisms within the coding region of bovine ATP1A1 gene. A novel C/A mutation was identified at the nucleotide position 2789 of the ATP1A1 mRNA, but it was silent with respect to the amino acid sequence of the protein. The LIS-SSCP banding pattern could be divided into three kinds of genotypes, named CC, CA, and AA. Also, the association of the novel SNP of ATP1A1 gene with heat tolerance traits was studied, we found that the individuals with genotype CC showed significantly higher heat resistance than those of genotype CA (P<0.05). Further, the mRNA levels of ATP1A1 gene in lymphocytes of peripheral blood in dairy cows among various temperature groups and genotypes were analyzed by using real-time RT-PCR. Results showed that the expression of ATP1A1 mRNA was highest in heat-stressed cows with CC genotype among the three genotypes (P<0.01), and the ATP1A1 mRNA level at temperature 32.5°C was higher than that at optimal temperature 12.5°C in dairy cows (P<0.01). Simultaneity, the plasma potassium (K+, mmol/l) and sodium (Na+, mmol/l) declined when the temperature dropped (P<0.05). Our findings implied that the novel SNP here could be a potential genetic marker for anti-heat stress trait in dairy cow breeding.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Liu Y,Li D,Li H,Zhou X,Wang Gdoi
10.1007/s11033-010-0080-8subject
Has Abstractpub_date
2011-01-01 00:00:00pages
83-8issue
1eissn
0301-4851issn
1573-4978journal_volume
38pub_type
杂志文章abstract::Chronic obstructive pulmonary disease (COPD) is a common, complex disorder associated with substantial morbidity and mortality, influenced by both environmental factor and genetic factor. ADAM33 gene was found to be associated with asthma, declined lung function and COPD. The purpose of the study was to test whether S...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0637-6
更新日期:2011-11-01 00:00:00
abstract::Azotobacter chroococcum and A. salinestris do not possess significant and distinct morphological and physiological differences and are often mistaken with each other in microbiological research. In this study, 12 isolates of Azotobacter isolated by standard protocol from soils were identified morphologically and physi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05133-7
更新日期:2020-01-01 00:00:00
abstract::The MinION is a miniaturized high-throughput next generation sequencing platform of novel conception. The use of nucleic acids derived from formalin-fixed paraffin-embedded samples is highly desirable, but their adoption for molecular assays is hurdled by the high degree of fragmentation and by the chemical-induced mu...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-017-4133-0
更新日期:2018-02-01 00:00:00
abstract::RNase MRP is a ribonucleoprotein enzyme with a structure similar to RNase P. It is required for normal processing of precursor rRNA, cleaving it in the Internal Transcribed Spacer 1. ...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/BF00988708
更新日期:1995-01-01 00:00:00
abstract::Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3633-4
更新日期:2014-11-01 00:00:00
abstract::Narrow-leafed lupin (Lupinus angustifolius L.) is used as grain legumes, fodder for livestock and green manure in the world and has a great potential to be developed as a new crop in China. In this study, we assessed the genetic diversity among a set of 109 newly introduced accessions of narrow-leafed lupin using 76 g...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05596-z
更新日期:2020-07-01 00:00:00
abstract::Infection with mycobacterium tuberculosis (MTB) can cause different outcomes in hosts with variant genetic backgrounds. Previously, we identified an intracellular pathogen resistance 1 (Ipr1) gene with the role of resistance of MTB infection in mice model. However, until now, its binding proteins have been little know...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0042-1
更新日期:2010-12-01 00:00:00
abstract::Knowledge in the area of genetic diversity could aid in providing useful information in the selection of material for breeding such as hybridization programs and quantitative trait loci mapping. To this end, 50 Nicotiana tabacum genotypes were genotyped with 21 primer combination of amplified fragment length polymorph...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3194-6
更新日期:2014-05-01 00:00:00
abstract::Glutamate represents the main excitatory neurotransmitter in the mammalian brain; however, its excessive elevation in the extracellular space is cytotoxic and can result in neuronal death. The ischemia initiated brain damage reflects changes in glutamate concentration in peripheral blood. This paper investigated the r...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05626-w
更新日期:2020-07-01 00:00:00
abstract::Substantial epidemiological studies have shown an association of obesity with the common gynecological malignancy, endometrial cancer. The relevant interactions and contribution of estradiol and the adipose cytokine, leptin, in endometrial lesions are not completely understood. Suitable animal models to understand the...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05116-8
更新日期:2020-01-01 00:00:00
abstract::Long term intake of high-glucose diet (HGD) may induce many diseases such as dyslipidemia, fatty liver and diabetes disease. Most of the research for molecular mechanisms of the association between HGD and the above diseases focus on the metabolism of glucose and lipid. However, there are few studies on molecular mech...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9622-3
更新日期:2010-04-01 00:00:00
abstract::The full length cDNA sequence of the Tpt1/TCTP (Tumor protein, Translationally-controlled1) gene was identified from Common Carp (Cyprinus carpio, Cyprinidae), and was designated as CcTpt1 gene. The CDS is 510 bp and encodes a 170-amino acid peptide with a typical Tpt1 signature 2 domain, and is a typical Tpt1 protein...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2806-x
更新日期:2013-10-13 00:00:00
abstract::The aim of this study was to determine whether the Glutathione S-transferase M1 (GSTM1) and P1 (GSTP1) polymorphisms confer susceptibility to rheumatoid arthritis (RA). Meta-analysis was performed on the associations between the GSTM1 and GSTP1 null genotypes and RA, and on the association between smoking or seroposit...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-1965-5
更新日期:2012-12-01 00:00:00
abstract::Kidney transplantation to treat end-stage renal disease has evolved rapidly from the first successful transplantations to the current widespread use of grafts from both cadaveric and living donors. But acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate pos...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1321-1
更新日期:2012-05-01 00:00:00
abstract::Mucor circinelloides is a dimorphic fungal model for studying several biological processes including cell differentiation (yeast-mold transitions) as well as biodiesel and carotene production. The recent release of the first draft sequence of the M. circinelloides genome, combined with the availability of analytical m...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3818-x
更新日期:2015-03-01 00:00:00
abstract::In view of high mortality associated with coronary artery disease (CAD), development of an early predicting tool will be beneficial in reducing the burden of the disease. The database comprising demographic, conventional, folate/xenobiotic genetic risk factors of 648 subjects (364 cases of CAD and 284 healthy controls...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4236-2
更新日期:2018-10-01 00:00:00
abstract::Allele frequencies for the high polymorphic short tandem repeats (STR) loci PentaE, PentaD, D18S51, D21S11, TH01, D3S1358, FGA, D16S539, D7820, D13S317, vWA and D81179 were analysed in an native Amerindian population from Mato Grosso do Sul state named Terena. Deviations from Hardy-Weinberg expectations were evaluated...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1234-z
更新日期:2012-04-01 00:00:00
abstract::An extensive study on spontaneous and 5-Fluorodeoxyuridine induced fragile sites identified Xq31 in cattle (Bos taurus) and (Xq24, Xq26) in sheep (Ovis aries) in addition to several autosomal fragile sites (under publication). A ZOO-FISH study using three cloned human fragile-X probes with CCG/CGG(n) trinucleotide rep...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9221-8
更新日期:2009-03-01 00:00:00
abstract::A full-length cDNA encoding wheat peroxisomal ascorbate peroxidase (pAPX) was cloned by Suppression Subtractive Hybridization (SSH) and in silico approach. The cDNA was 1027 bp in length and contained a complete ORF of 876 bp, which encodes a protein of 292 amino acid residues. Its deduced amino acids sequence had 84%...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-005-4536-1
更新日期:2006-09-01 00:00:00
abstract::BACs-on-Beads (BoBs™) assay is a rapid aneuploidy test (RAT) that detects numerical chromosomal aneuploidies and multiple microdeletion/microduplication syndromes. This study was conducted to appraise the usefulness of the BoB™ assay as a complementary diagnostic tool to conventional karyotyping for the rapid detectio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05117-7
更新日期:2020-01-01 00:00:00
abstract::Calcific mitral valve stenosis (MVS) is a common disease characterized by extensive remodeling of the extracellular matrix via matrix metalloproteinases (MMPs). The mechanism of calcification due to extensive matrix remodeling remains unclear. In this study, we investigated the relationship between MMP-3, tissue inhib...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4372-8
更新日期:2018-12-01 00:00:00
abstract::CREB-regulated transcription coactivator 3 (CRTC3) was a recently identified protein which played an important role in glucose and lipid metabolism. Previous research showed that the polymorphisms of CRTC3 were associated with obesity in Mexican-Americans. Data on that relationship in Chinese was unavailable so far. S...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2844-4
更新日期:2014-01-01 00:00:00
abstract::CENP-A locates at nucleosome as histone H3-like proteins, and is phosphorylated during mitosis. We investigated the dynamic distribution of p-CENP-A to explore the details of its function. We found that p-CENP-A was phosphorylated at late prophase, and the signal of p-CENP-A arranged at equatorial plate along with nuc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9136-9
更新日期:2008-12-01 00:00:00
abstract::The aim of this work was to study β-defensin 1 (SBD1) and β-defensin 2 (SBD2) genes in Valle del Belice dairy sheep in order to identify polymorphisms that can be utilized as markers of the analyzed genes, and search for the functional effects and roles of the identified polymorphisms (variation of the amino acid sequ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0694-5
更新日期:2011-11-01 00:00:00
abstract::WRKY transcription factor proteins play significant roles in plant stress responses. Here, we report the cloning and characterization of a novel WRKY gene, MusaWRKY71 isolated from an edible banana cultivar Musa spp. Karibale Monthan (ABB group). MusaWRKY71, initially identified using in silico approaches from an abio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0521-4
更新日期:2011-08-01 00:00:00
abstract::Various investigations have been so far performed for extraction of genomic DNA from plant tissues, in which the extracted intact DNA can be exploited for a diverse range of biological studies. Extraction of high quality DNA from leathery plant tissues (e.g., coniferous organs) appears to be a critical stage. Moreover...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9634-z
更新日期:2010-02-01 00:00:00
abstract::By measuring the permeability across the lipid bilayer in the presence and absence of membrane-bound protein or glycoprotein it should be possible to get an impression concerning their ability to penetrate into the membrane bilayer. Proteins such as phospholipase A2 and acetylcholinesterase markedly increase the perme...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00777245
更新日期:1982-04-16 00:00:00
abstract:OBJECTIVES:Glucose transporter (GLUT) 4 is a major mediator of blood glucose levels and a key regulator of whole-body glucose homeostasis. This study aimed at evaluating the function of goat GLUT4 on glucose absorption and the effect of GLUT4 on lactose synthesis in goat mammary gland epithelial (GMGE) cells. METHODS:...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2915-6
更新日期:2014-02-01 00:00:00
abstract::Common bean is gaining acceptance as one of the most valuable major food consumed worldwide owing to innumerable nutritional and therapeutic benefits. Comparatively less productivity in underdeveloped countries encouraged us to proceed for QTL mining of yield traits in common bean. Heretofore, multiple yield associate...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05735-6
更新日期:2020-09-01 00:00:00
abstract::Abnormally protein aggregation and deposition are key pathological features of ALS, which may related with dysfunctional cellular autophagy. In the current study, we found that, compared with wtSOD1 cells, serum starvation treatment resulted in significant higher percentage of apoptosis in mutSOD1 cells; Lithium treat...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05071-4
更新日期:2019-12-01 00:00:00