Abstract：
:Mucor circinelloides is a dimorphic fungal model for studying several biological processes including cell differentiation (yeast-mold transitions) as well as biodiesel and carotene production. The recent release of the first draft sequence of the M. circinelloides genome, combined with the availability of analytical methods to determine patterns of gene expression, such as quantitative Reverse transcription-Polymerase chain reaction (qRT-PCR), and the development of molecular genetic tools for the manipulation of the fungus, may help identify M. circinelloides gene products and analyze their relevance in different biological processes. However, no information is available on M. circinelloides genes of stable expression that could serve as internal references in qRT-PCR analyses. One approach to solve this problem consists in the use of housekeeping genes as internal references. However, validation of the usability of these reference genes is a fundamental step prior to initiating qRT-PCR assays. This work evaluates expression of several constitutive genes by qRT-PCR throughout the morphological differentiation stages of M. circinelloides; our results indicate that tfc-1 and ef-1 are the most stable genes for qRT-PCR assays during differentiation studies and they are proposed as reference genes to carry out gene expression studies in this fungus.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Valle-Maldonado MI,Jácome-Galarza IE,Gutiérrez-Corona F,Ramírez-Díaz MI,Campos-García J,Meza-Carmen Vdoi
10.1007/s11033-014-3818-xsubject
Has Abstractpub_date
2015-03-01 00:00:00pages
705-11issue
3eissn
0301-4851issn
1573-4978journal_volume
42pub_type
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