当前位置： SCI文献检索 > MOLECULAR BIOLOGY REPORTS期刊下所有文献 > Functional characterization of a Δ5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve.

Functional characterization of a Δ5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve.

Abstract：

:Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys nobilis cDNA with high homology to vertebrate FADs. Functional characterization in recombinant yeast Saccharomyces cerevisiae showed that scallop FAD exhibited Δ5-desaturation activity towards both saturated and PUFA substrates. Thus, it efficiently desaturated exogenously added PUFA C20:4(n - 3) and C20:3(n - 6) to C20:5(n - 3) (EPA) and C20:4(n - 6) (ARA) respectively. It also converted the yeast's endogenous C18:0 into C18:1(n - 13), and participated in the biosynthesis of non-methylene-interrupted FA by introducing a double bond to C20:3(n - 3) and C20:2(n - 6) in the Δ5 carbon. Temporal transcript profile of scallop FAD was studied during early embryonic development. High level of mRNA was found at the beginning of embryogenesis (egg) and noticeable decreases of were observed during larvae development, suggesting maternal FAD mRNA transfer to the embryo. Further, FAD transcripts were detected in all tissues analyzed, with the gonad and hepatopancreas showing the highest expression.

journal_name

Mol Biol Rep

journal_title

Molecular biology reports

authors

Liu H,Guo Z,Zheng H,Wang S,Wang Y,Liu W,Zhang G

doi

10.1007/s11033-014-3633-4

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

7437-45

issue

11

eissn

0301-4851

issn

1573-4978

journal_volume

41

pub_type

杂志文章

相关文献

MOLECULAR BIOLOGY REPORTS文献大全
  • Human intracerebroventricular (ICV) injection of autologous, non-engineered, adipose-derived stromal vascular fraction (ADSVF) for neurodegenerative disorders: results of a 3-year phase 1 study of 113 injections in 31 patients.

    abstract：:We have chosen to test the safety of human intracerebroventricular (ICV) brain injections of autologous non-genetically-modified adipose-derived stromal vascular fraction (ADSVF). In this IRB-approved trial, 24 patients received ICV ADSVF via an implanted reservoir between 5/22/14 and 5/22/17. Seven others were inject...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04983-5

    authors： Duma C,Kopyov O,Kopyov A,Berman M,Lander E,Elam M,Arata M,Weiland D,Cannell R,Caraway C,Berman S,Scord K,Stemler L,Chung K,Khoudari S,McRory R,Duma C,Farmer S,Bravo A,Yassa C,Sanathara A,Singh E,Rapaport B

    更新日期：2019-10-01 00:00:00

  • Selective interactions of hnRNP M isoforms with the TET proteins TAF15 and TLS/FUS.

    abstract：:The molecular composition of macromolecular assemblies engaged in transcription and splicing influences biogenesis of mRNA transcripts. Preference for one over the other interactive protein partner within those complexes is expected to change the gene expression pattern and to affect subsequent cellular events. We rep...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3128-3

    authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

    更新日期：2014-01-01 00:00:00

  • Molecular phylogeny and biogeography of lac insects (Hemiptera: Kerriidae) inferred from nuclear and mitochondrial gene sequences.

    abstract：:Lac insects are commercial scale insects with high economic value. The combined molecular phylogeny of 20 lac insect populations was generated using elongation factor 1 alpha, mitochondrial cytochrome c oxidase subunit I and the small subunit ribosomal RNA gene loci. The 20 populations of lac insects clustered into fo...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-013-2701-5

    authors： Chen H,Chen X,Feng Y,Yang H,He R,Zhang W,Yang Z

    更新日期：2013-10-01 00:00:00

  • Immunity augmentation in Botia dario (Hamilton, 1822) through carotenoid: a dietary approach.

    abstract：:The immunity and health status of ornamental fish is an important aspect, as they are kept in a confined environment and various stressful conditions which lead to depletion of overall colourful appearance and mortality. The carotenoids can act as immunity boosters in captive aquarium system and may be supplemented in...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-05027-8

    authors： Tripathy PS,Khatei A,Parhi J,Choudhury TG,Sahoo L,Mandal SC

    更新日期：2019-12-01 00:00:00

  • Knocking down Wnt9a mRNA levels increases cellular proliferation.

    abstract：:Wnts are secreted lipid-modified signaling proteins. Activation of Wnt signalling in many tissues has also been associated with cancer. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this st...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-007-9055-9

    authors： Xiang Y,Lin G,Zhang Q,Tan Y,Lu G

    更新日期：2008-06-01 00:00:00

  • Label-free quantitative proteomics analysis of dormant terminal buds of poplar.

    abstract：:Induction and break of bud dormancy are important features for perennial plants surviving extreme seasonal variations in climate. However, the molecular mechanism of the dormancy regulation, still remain poorly understood. To better understand the molecular basis of poplar bud dormancy, we used a label-free quantitati...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-013-2548-9

    authors： Ning DL,Liu CC,Liu JW,Shen Z,Chen S,Liu F,Wang BC,Yang CP

    更新日期：2013-07-01 00:00:00

  • Correction to: Preparation, optimization and pharmacological evaluation of 99mTc-4-hydroxy-3-(2-hydroxy-3-methoxybenzylideneamino) benzoic acid complex: as a novel potential radiopharmaceutical agent with hepatobiliary excretion.

    abstract：:In the published original version, the complete list of authors was omitted. The complete list of contributing authors is updated with this Correction. ...

    journal_title：Molecular biology reports

    pub_type： 已发布勘误

    doi：10.1007/s11033-018-4454-7

    authors： Akbar MU,Bokhari TH,Roohi S,Zia KM,Ahmad MR,Hina S,Sohaib M

    更新日期：2018-12-01 00:00:00

  • Mitochondrial activity in different regions of the brain at the onset of streptozotocin-induced diabetes in rats.

    abstract：:Diabetes affects a variety of tissues including the central nervous system; moreover, some evidence indicates that memory and learning processes are disrupted. Also, oxidative stress triggers alterations in different tissues including the brain. Recent studies indicate mitochondria dysfunction is a pivotal factor for ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-018-4233-5

    authors： Osorio-Paz I,Ramírez-Pérez G,Hernández-Ramírez JE,Uribe-Carvajal S,Salceda R

    更新日期：2018-10-01 00:00:00

  • Single and multiple CH (calponin homology) domain containing multidomain proteins in Arabidopsis and Saccharomyces: an inventory.

    abstract：:Genes for individual domains such as CH, lim, ankyrin, PH and RhoGAP, IQ motif, Ig_FLMN, spectrin, and EF hand probably existed in early evolution before there were plants, fungi or animals so that when we examine multidomain proteins in Arabidopsis, Saccharomyces, Dictyostelium or Homo Sapiens we encounter various co...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0097-z

    authors： Friedberg F

    更新日期：2011-01-01 00:00:00

  • Isolation and dynamic expression of four genes involving in shikimic acid pathway in Camellia sinensis 'Baicha 1' during periodic albinism.

    abstract：:Flavonoids are the main flavor components and functional ingredients in tea, and the shikimic acid pathway is considered as one of the most important pathways in flavonoid biosynthesis, but little was known about the function of regulatory genes in the metabolism phenolic compounds in tea plant (Camellia sinensis), es...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-016-4045-4

    authors： Zhu XJ,Zhao Z,Xin HH,Wang ML,Wang WD,Chen X,Li XH

    更新日期：2016-10-01 00:00:00

  • The polymorphism analysis of CD169 and CD163 related with the risk of porcine reproductive and respiratory syndrome virus (PRRSV) infection.

    abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) could infect porcine alveolar macrophages (PAM), and the CD169 and CD163 are identified as critical receptors on the surface of PAM, but whether the single nucleotide polymorphisms (SNPs) of these genes could influence the infection is remain unclear. In this...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1857-8

    authors： Ren YW,Zhang YY,Affara NA,Sargent CA,Yang LG,Zhao JL,Fang LR,Wu JJ,Fang R,Tong Q,Xiao J,Li JL,Jiang YB,Chen HC,Zhang SJ

    更新日期：2012-11-01 00:00:00

  • Transcriptome sequencing reveals genetic mechanisms underlying the transition between the laying and brooding phases and gene expression changes associated with divergent reproductive phenotypes in chickens.

    abstract：:Transition from laying to incubation behavior in chicken is an interesting topic in reproductive biology. The decline of incubation behavior in chicken population has led to considerable phenotypic differences in reproductive traits between breeds. However, the exact genetic mechanism of the reproductive phase transit...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-016-4033-8

    authors： Shen X,Bai X,Xu J,Zhou M,Xu H,Nie Q,Lu X,Zhang X

    更新日期：2016-09-01 00:00:00

  • Phytopathogenic oomycetes: a review focusing on Phytophthora cinnamomi and biotechnological approaches.

    abstract：:The Phytophthora genus is composed, mainly, of plant pathogens. This genus belongs to the Oomycete class, also known as "pseudo-fungi", within the Chromista Kingdom. Phytophthora spp. is highlighted due to the significant plant diseases that they cause, which represents some of the most economically and cultural losse...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,评审

    doi：10.1007/s11033-020-05911-8

    authors： de Andrade Lourenço D,Branco I,Choupina A

    更新日期：2020-11-01 00:00:00

  • Comparative study of the interactions between ovalbumin and three alkaloids by spectrofluorimetry.

    abstract：:The interaction between ovalbumin (OVA) and three purine alkaloids (caffeine, theophylline and diprophylline) was investigated by the aid of intrinsic and synchronous fluorescence, ultraviolet-vis absorbance, resonance light-scattering spectra and three-dimensional fluorescence spectra techniques. Results showed that ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-2418-x

    authors： Wang RQ,Yin YJ,Li H,Wang Y,Pu JJ,Wang R,Dou HJ,Song CJ,Wang RY

    更新日期：2013-04-01 00:00:00

  • Cloning, expression and characterization of a novel human VMP gene.

    abstract：:We report here cloning and characterization of a novel human gene, termed VMP, which is a vesicular membrane protein. RT-PCR analysis shows that VMP is expressed exclusively in brain of the 16 tissues examined, suggesting that it is a neuron-specific membrane protein. The cDNA encodes 195 amino acid with a putative mo...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1023/a:1020402410522

    authors： Cheng C,Xu J,Ye X,Dai J,Wu Q,Zeng L,Wang L,Zhao W,Ji C,Gu S,Xie Y,Mao Y

    更新日期：2002-09-01 00:00:00

  • Prevalence of GJB2 (CX26) gene mutations in south Iranian patients with autosomal recessive nonsyndromic sensorineural hearing loss.

    abstract：:Hereditary hearing loss is a genetically heterogeneous disorder. Mutations in connexin 26 (CX26), are a major cause in many countries and are largely dependent on ethnic groups. The purpose of our study was to evaluate the prevalence of GJB2 mutations among affected individuals from south of Iran. Fifty patients prese...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1929-9

    authors： Hashemi SB,Ashraf MJ,Saboori M,Azarpira N,Darai M

    更新日期：2012-12-01 00:00:00

  • Imprinting analysis of porcine DIO3 gene in two fetal stages and association analysis with carcass and meat quality traits.

    abstract：:Imprinted genes play important roles in mammalian growth, development and behavior. In this study, we obtained 1568 bp mRNA sequence of porcine DIO3 (deiodinase, iodothyronine, type III), and also identified its imprinting status during porcine fetal development. The complete open reading frame (ORF) encoding 278 amin...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-0983-z

    authors： Qiao M,Wu HY,Guo L,Mei SQ,Zhang PP,Li FE,Zheng R,Deng CY

    更新日期：2012-03-01 00:00:00

  • RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.

    abstract：:Methyltransferases play essential roles in modulating important cellular and metabolic processes. A mouse putative N6-DNA methyltransferase gene (GenBank No AY456393) is a novel gene named mN6amt1(mN6A1). To investigate its function in cell fate and protein translation, RNA interference (RNAi)-mediated knock-down meth...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-008-9243-2

    authors： Liu Y,Nie D,Huang Y,Lu G

    更新日期：2009-04-01 00:00:00

  • Differential expression of TRPC channels in the left ventricle of spontaneously hypertensive rats.

    abstract：:This study was designed to identify the differential expression of the canonical transient receptor potential (TRPC) channels in the left ventricle of spontaneously hypertensive rats (SHR). Echocardiography studies were performed to compare the left ventricular function in SHR vs. Wistar-Kyoto rats (WKY), and the mRNA...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-009-9792-z

    authors： Liu FF,Ma ZY,Li DL,Feng JB,Zhang K,Wang R,Zhang W,Li L,Zhang Y

    更新日期：2010-07-01 00:00:00

  • Overexpressed in colorectal carcinoma gene (OCC-1) upregulation and APPL2 gene downregulation in breast cancer specimens.

    abstract：:Breast cancer is the most common cancer type and the second cause of cancer death in women. Different mechanisms are contributed to the initiation and progression of the breast cancer. OCC-1 and APPL2 neighboring genes located in 12q.23.3 human chromosome region are related to colorectal cancer. Here, we intended to i...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-018-4336-z

    authors： Ghalaei A,Kay M,Zarrinfam S,Hoseinpour P,Behmanesh M,Soltani BM

    更新日期：2018-12-01 00:00:00

  • The role of oxidative stress as a risk factor for rupture of posterior inferior cerebellar artery aneurysms.

    abstract：:Cerebral aneurysm affects 2-5% of the population and posterior inferior cerebellar artery (PICA) aneurysms account for 1-3% of all intracranial aneurysms. Oxidative stress is known to contribute to the progression of cerebrovascular disease and it may be increased by inflammation, a key contributor to cerebral aneurys...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-018-4374-6

    authors： Šćepanović V,Tasić G,Repac N,Nikolić I,Janićijević A,Todorović D,Stojanović M,Šćepanović R,Mitrović D,Šćepanović T,Borozan S,Šćepanović L

    更新日期：2018-12-01 00:00:00

  • Transcript profiling of chickpea pod wall revealed the expression of floral homeotic gene AGAMOUS-like X2 (CaAGLX2).

    abstract：:The differentially expressed genes in the chickpea pod wall have been identified for the first time using a forward suppression subtractive hybridization (SSH) library. In all, 226 clones of SSH library were sequenced and analyzed. A total of 179 high-quality expressed sequence tags (ESTs) were generated and based on ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-05005-0

    authors： Vasantrao JM,Baruah IK,Panda D,Bhattacharjee M,Acharjee S,Sarmah BK

    更新日期：2019-12-01 00:00:00

  • Blocking P2X7 receptor ameliorates oxidized LDL-mediated podocyte apoptosis.

    abstract：:The purpose of our research is to elucidate whether oxLDL activates P2X7R in cultured human podocytes and if the activation of P2X7R leads to podocyte apoptosis. Additionally, we explore the underlying mechanism involved in podocyte apoptosis. Immortalized human podocytes were incubated with oxLDL (80 µg/ml), P2X7R an...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04823-6

    authors： Zhu Y,Li Q,Xun W,Chen Y,Zhang C,Sun S

    更新日期：2019-08-01 00:00:00

  • Molecular insight into the metabolic activities of a protein-rich micro alga, Arthrospira platensis by de novo transcriptome analysis.

    abstract：:To gain genetic insights into the protein-rich microalga, the transcriptome of Arthrospira platensis was sequenced using Illumina technology and de novo assembly was carried out. A total of 6023 transcripts were present in the transcriptome among which 4616 transcripts were annotated with specific functions. Gene onto...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-018-4229-1

    authors： Kumaresan V,Sannasimuthu A,Arasu MV,Al-Dhabi NA,Arockiaraj J

    更新日期：2018-10-01 00:00:00

  • Sequence variation in promoter regions of genes for CC chemokine ligands (CCL)19 and 21 in Czech patients with myocardial infarction.

    abstract：:Recruitment of inflammatory cells to the arterial wall is an important pathogenic mechanism of atherosclerosis and coronary artery disease (CAD). Functional variability in the genes encoding for chemokines that promote infiltration of atherosclerotic plaques by macrophages and lymphocytes may therefore contribute to t...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3175-9

    authors： Stahelova A,Petrkova J,Petrek M,Mrazek F

    更新日期：2014-05-01 00:00:00

  • Studies on resources of genetic diversity in conservative flocks of geese using microsatellite DNA polymorphic markers.

    abstract：:The studies conducted aimed at evaluating the genetic diversity within and between varieties of conservative flocks of geese, using the polymorphism of 14 microsatellite sequences. The experimental material included conservative flocks of geese the following indigenous breeds and varieties kept in Poland: Kielecka (Ki...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-1327-8

    authors： Parada R,Książkiewicz J,Kawka M,Jaszczak K

    更新日期：2012-05-01 00:00:00

  • Modulatory effects of Amukkara Choornam on Candida albicans biofilm: in vitro and in vivo study.

    abstract：:In the present study, Amukkara Chooram (AC) a well known herbal medicine was investigated for their antibiofilm efficacy against biofilm of Candida albicans. The biofilm inhibitory concentration of 20 µg/mL of AC showed promising effect by inhibiting the biofilm upto 60%. Morphogenic transition state of C. albicans fr...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04757-z

    authors： Rajamohamed BS,Siddharthan S

    更新日期：2019-06-01 00:00:00

  • Haemoglobin messenger RNA in spleen of anaemic rabbits.

    abstract：:Haemoglobin meseenger RNA was isolated from spleen erythroïd cells of anaemic rabbits. The mRNA fraction looks quite homogeneous when analyzed by sucrose gradient centrifugation; it sediments in the 9S region. When added to an ascites cell free system, spleen 9S RNA stimulates the incorporation of radioactive leucine ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00357405

    authors： Nokin P,Gautier E

    更新日期：1973-06-01 00:00:00

  • Molecular cloning and characterization of a 2C-methyl-D: -erythritol 2,4-cyclodiphosphate synthase gene from Cephalotaxus harringtonia.

    abstract：:The full-length MECPS cDNA sequence (designated as Chmecps, GenBank Accession No.: DQ415658) was isolated by rapid amplification of cDNA ends (RACE) for the first time from Cephalotaxus harringtonia. The full-length cDNA of Chmecps was 1,146 bp containing a 753 bp open reading frame (ORF) encoding a polypeptide of 250...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-008-9377-2

    authors： Chen J,Xiao Y,Di P,Yu X,Chen W,Zhang L

    更新日期：2009-09-01 00:00:00

  • Identification of novel single nucleotide polymorphisms (SNPs) of the lipoprotein lipase (LPL) gene associated with fatty acid composition in Korean cattle.

    abstract：:The lipoprotein lipase (LPL) gene can be considered a functional candidate gene that regulates fatty acid composition. In this study, genetic associations between fatty acid composition and exonic single nucleotide polymorphisms (SNPs) in the LPL gene were examined using 612 Korean cattle. We investigated the relation...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-2389-y

    authors： Oh D,La B,Lee Y,Byun Y,Lee J,Yeo G,Yeo J

    更新日期：2013-04-01 00:00:00